As the main component of terrestrial ecosystem, vegetation plays a very important role in regional ecosystem environmental change, global carbon cycle and climate regulation. The Lower Mekong region (LMR) is at the core of Southeast Asia, its vegetation changes will affect the regional ecosystem and climate. Five countries of LMR were selected as the study area, based on MODIS (Moderate-Resolution Imaging Spectroradiometer) NDVI(Normal Difference Vegetation Index) data from 2000 to 2022, using the Sen’s slope estimator, Mann–Kendall trend test and geographic detector to study the spatial and temporal variation trends and driving forces of vegetation coverage. The results showed that:(1) From 2000 to 2022,the vegetation coverage in the LMR showed an overall fluctuating upward trend, the annual average Fractional Vegetation Cover(FVC) value was 0.70, mainly with high vegetation coverage and relatively high vegetation coverage. Vegetation distribution had obviously spatial heterogeneity, and the vegetation of Myanmar, Laos and Vietnam was significantly larger than Thailand and Cambodia.(2) The variation trend analysis of Sen_MK showed that the proportion of improved and degraded vegetation coverage areas in the LMR were 56.33% and 37.55% respectively. The vegetation improvement area was much larger than the vegetation degradation area during 2000–2022. According to the variation trend analysis of different countries, the vegetation coverage improvement area in Vietnam, Myanmar and Thailand were larger than the degraded , the overall vegetation coverage variation trend were good. However, in Laos and Cambodia, the degraded areas were larger than the improved, the overall variation trends of coverage were not good.(3) The results of geographic detector showed that the Land Use and Land Cover(LULC) had the greatest influence on vegetation coverage in the study area.The influencing factors of vegetation coverage were different in the LMR. For Vietnam, Thailand and Laos,elevation and slope factors were second only to LULC, for Myanmar and Cambodia, the influence of precipitation factor was second only to LULC. The results provide scientific data support for understanding the ecological environment status and future changes in the research area.