Vegetable Protein Sources Research Articles

Metagenomic Insights into Enhancing Protein Content and Digestibility in Jack Bean (Canavalia ensiformis) Tempeh: Unraveling Microbial Dynamics During Fermentation

Jack beans (Canavalia ensiformis) are a locally abundant Indonesian bean variety boasting a significant protein content that remains underutilized. The production of tempeh from Jack beans presents a promising avenue for tapping into this valuable vegetable protein source. The tempeh-making process involves several key stages, including soaking the ingredients, inoculating with a mold starter, and fermenting the mixture. Throughout each stage, protein content preservation and effective utilization are paramount. This study uses high-throughput sequencing technologies to characterize protein hydrolysis in Jack beans during fermentation. The research aims to investigate how microorganisms participating in the fermentation of Jack bean tempeh influence protein content and protein digestibility. The findings reveal that prolonging the fermentation time of Jack beans, utilizing a 1.5% tempeh mold starter, and fermenting for 36 hours resulted in a notable increase in protein content by 66.63% and enhanced protein digestibility in vitro by 48.82%. This improvement may be attributed to the predominant activities of Bacillus and Rhizopus during the fermentation process by the metagenomics approach.

Revealing the effect of hydrothermal processing of legumes on the accumulation of dry matter in aquafaba

Legumes are an affordable source of vegetable protein, fiber, and vitamins, and due to their high nutritional and biological value, they are widely used in the nutrition of various categories of consumers. The main methods of processing legumes include hydromechanical and hydrothermal treatment, which is aimed at achieving culinary readiness of boiled legumes. Studying the parameters of the specified technological process for the accumulation of dry substances in aquafaba could make it possible to influence the composition and properties of this liquid, which would provide opportunities for its use in the food industry. The influence of hydrothermal processing of leguminous grains on the accumulation of dry matter in aquafaba, as well as the kinetics of moisture content of various types of leguminous grains, have been experimentally investigated, which made it possible to determine the rational parameters of hydromechanical and hydrothermal processing of chickpeas, beans, peas, lentils, with the preparation of boiled leguminous grains and aquafaba. It was determined that during hydromechanical processing intensive moisture absorption occurs in the first hours and reaches up to 80 % of the initial mass, after which the intensity of water absorption decreases somewhat. Rational technological parameters of hydrothermal processing of leguminous grains by two methods, basic (cooking at a temperature of 99±1 °С) and under pressure (cooking in a pressure cooker at a temperature of 120±1 °С) were established in the relationship "achieving the readiness of legumes – the content of dry substances in aquafaba". The use of the above-mentioned techniques for hydrothermal processing of leguminous grains make it possible to obtain the content of dry substances in aquafaba in the range of 2.8–4.8 % for cooking by the main technique and 3.9–7.0 % under pressure cooking conditions. To understand the processes regarding the influence of hydrothermal processing of legume grains on the accumulation of dry substances, the Peleg model was used. The experimental data could be used to substantiate parameters for the technological process to produce foods based on legumes

Aplikasi Pupuk Branglai (Brangkasan Kedelai) dalam Upaya Peningkatan Pertumbuhan dan Produksi Beberapa Varietas Tanaman Kedelai (Glycine max L.)

Soybean (Glycine max L.), which is widely grown in Indonesia, is a popular food crop because of its high protein content. This plant plays an important role in increasing protein intake for the community, because it is a safe and economically affordable source of vegetable protein. To increase the productivity of soybean plants, it is not only important to choose quality seed varieties, but also to implement appropriate cultivation practices. One of the main techniques is to use superior seed varieties. Apart from that, increasing the quality and yield of soybean production can be achieved by providing fertilizer that meets the nutritional needs of plants. This research used a Randomized Block Design (RAK) with 2 factors and 2 replications to obtain 24 research plots. Factor I of Branglai Fertilizer (B) which consists of 4 levels,namely: B0=0ml/liter.air/plant, B1=20ml/liter.air /polybag, B2=40 ml/liter.air/polybag, B3 = 60 ml/ liter. water/polybag. Factor II Soybean Variety (V) which consists of 3 levels, namely: V1 = Dega 1 Variety, V2 = Ring 1 Variety, V3 = Devon 1 Variety. The research procedure consists of making fertilizer, land preparation, filling polybags, planting, fertilizer application branglai (soybean stover), determination of sample plants, maintenance and harvest. Keywords: soybean, branglai fertilizer, soybean variety

Have We Selected for Higher Mesophyll Conductance in Domesticating Soybean?

Soybean (Glycine max) is the single most important global source of vegetable protein. Yield improvements per unit land area are needed to avoid further expansion onto natural systems. Mesophyll conductance (gm) quantifies the ease with which CO2 can diffuse from the sub-stomatal cavity to Rubisco. Increasing gm is attractive since it increases photosynthesis without increasing water use. Most measurements of gm have been made during steady-state light saturated photosynthesis. In field crop canopies, light fluctuations are frequent and the speed with which gm can increase following shade to sun transitions affects crop carbon gain. Is there variability in gm within soybean germplasm? If so, indirect selection may have indirectly increased gm during domestication and subsequent breeding for sustainability and yield. A modern elite cultivar (LD11) was compared with four ancestor accessions of Glycine soja from the assumed area of domestication by concurrent measurements of gas exchange and carbon isotope discrimination (∆13C). gm was a significant limitation to soybean photosynthesis both at steady state and through light induction but was twice the value of the ancestors in LD11. This corresponded to a substantial increase in leaf photosynthetic CO2 uptake and water use efficiency.

Genetic Variation and QTL Analysis of Crude Fibre and Quality Traits in the Doubled Haploid Winter Oilseed Rape (Brassica napus L.) Population R19 x Lord

ABSTRACTThe oil‐extracted meal of oilseed rape is used for feeding livestock and is a potential source of vegetable protein for human consumption. Its protein quality is limited by glucosinolates, phenolic compounds, phytates and crude fibres. The reduction of cellulose, hemicellulose and indigestible lignin in black‐seeded oilseed rape may enhance the protein content, colour, sensory quality and digestibility. The objective of this study was to analyse the inheritance of seed fibre components in relation to oil, protein and other quality traits in a DH population derived from the cross of low lignin lines R19 and cv. Lord. The population of 143 lines was tested in field experiments in six environments and was genotyped with Illumina 15K Brassica SNP chip. QTL and candidate genes were identified using the high‐quality long‐read reference genome of Express 617. Major QTL for fibre components were identified on chromosomes C01 and C03 with opposite additive and epistatic effects. Fibre QTL colocated with each other and QTL for seed colour, oil and protein. Epistatic effects were identified, which resulted in a further decrease of fibre content.

Morphological Assessment of Drought Stress Tolerant Soybean Genotypes in Bangladesh

Soybean (Glycine max (L.) Merr) is the most important commercial grain legume and oil seed crop which represents the most important plant source of vegetable oil and protein in the world. The water stress tolerance of the fifty soybean genotypes was evaluated in a glasshouse at the Bangladesh Institute of Nuclear Agriculture (BINA). The study was carried out to identify the drought tolerant soybean genotype(s) for improving yield under rainfed conditions in Bangladesh. Drought stress was induced by withholding water completely from 21 days after emergence. The water stress was continued until wilting symptom persisted on plant overnight. Water stress caused an overall reduction in some morphological and yield contributing characters such as plant hight, number of branches plant-1, internode length, pod plant-1, seeds pod-1, hundred (100) seeds weight and finally the seed yield of soybean. The studied genotypes were categorized into four groups based on their yield reduction under drought stress conditions. Five genotypes YESOY-4, PK-416, Shohag, SBM-09 and Binasoybean-6 were categorized as tolerant (<35% yield reduction), fourteen genotypes were categorized as moderately tolerant (35%-60% yield reduction), twenty five genotypes were categorized as moderately susceptible (61%-80% yield reduction) and rest six genotypes were categorized as susceptible (>80% yield reduction). These five genotypes YESOY-4, PK-416, Shohag, SBM-09 and Binasoybean-6 study further for developing drought tolerant soybean verities which may be helpful for quality oil seed production.

Биохимический, минеральный и витаминный состав семян льна масличного белорусской селекции

Linseeds are a promising source of biologically valuable substances, essential amino acids, dietary fiber, vitamins, antioxidants, and essential polyunsaturated fatty acids. The article describes the biochemical, mineral, and vitamin composition of Belarusian flax varieties. The research objective was to select cultivars for functional food production. The study featured ten linseed cultivars, i.e., Ilim, Opus, Brestskiy, Salut, Fokus, Allyans, Dar, Vizir, Slavyanin, and Bonus. In 2020–2021, the authors planted plots of 1 m2 in triplicate for each cultivar. The experimental field belonged to the Institute of Flax, Ustye, Republic of Belarus. The study followed standar d methods to determine the content of micronutrients. All flax varieties proved to be a reliable source of vegetable fat (≤44.8%), protein (≤25.1%), and fiber (≤26.0%). The maximal content of α-linolenic acid belonged to Salut (63.0%), Vizir (61.4%), and Bonus (61.8%). The average mineral content was as follows: 7,500.0–10,625.0 mg/kg potassium, 5,700.0–7,522.5 mg/kg phosphorus, 2,300.0–2,605.0 mg/kg magnesium, 1,200.0–1,922.5 mg/kg calcium, 45.0–56.0 mg/kg iron, and 124.5–190.0 mg/kg sodium. The maximal content of vitamin B1 was registered in the samples of Salut (0.91 mg/100 g) and Brestskiy (1.08 mg/100 g); the maximal content of vitamin B2 belonged to the samples of Dar (0.058 mg/100 g), Ilim (0.057 mg/100 g), and Brestskiy (0.056 mg/100 g); the maximal content of vitamin E (tocopherol) was found in the samples of Salut (12.6 mg/100 g), Dar (12.07 mg/100 g), and Opus (12.35 mg/100 g). The linseeds were not suitable for high-quality food flour production after room temperature storage because the acid number increased by 64–73%. The excellent biological value of Belarusian flax cultivars demonstrated good prospects as highly active functional ingredients in new functional foods.

Enhancing Functional Properties and Protein Structure of Almond Protein Isolate Using High-Power Ultrasound Treatment.

The suitability of a given protein for use in food products depends heavily on characteristics such as foaming capacity, emulsifiability, and solubility, all of which are affected by the protein structure. Notably, protein structure, and thus characteristics related to food applications, can be altered by treatment with high-power ultrasound (HUS). Almonds are a promising source of high-quality vegetable protein for food products, but their physicochemical and functional properties remain largely unexplored, limiting their current applications in foods. Here, we tested the use of HUS on almond protein isolate (API) to determine the effects of this treatment on API functional properties. Aqueous almond protein suspensions were sonicated at varying power levels (200, 400, or 600 W) for two durations (15 or 30 min). The molecular structure, protein microstructure, solubility, and emulsifying and foaming properties of the resulting samples were then measured. The results showed that HUS treatment did not break API covalent bonds, but there were notable changes in the secondary protein structure composition, with the treated proteins showing a decrease in α-helices and β-turns, and an increase in random coil structures as the result of protein unfolding. HUS treatment also increased the number of surface free sulfhydryl groups and decreased the intrinsic fluorescence intensity, indicating that the treatment also led to alterations in the tertiary protein structures. The particle size in aqueous suspensions was decreased in treated samples, indicating that HUS caused the dissociation of API aggregates. Finally, treated samples showed increased water solubility, emulsifying activity, emulsifying stability, foaming capacity, and foaming stability. This study demonstrated that HUS altered key physicochemical characteristics of API, improving critical functional properties including solubility and foaming and emulsifying capacities. This study also validated HUS as a safe and environmentally responsible tool for enhancing desirable functional characteristics of almond proteins, promoting their use in the food industry as a high-quality plant-based protein.

Caracterización de lenteja y jacinto de agua, efecto en la digestibilidad aparente en la tilapia

Objective. To evaluate the nutritional composition of duckweed (DW) (Lemna minor) and water hyacinth (WH) (Eichhornia crassipes) and its effect on the apparent digestibility of dry matter (%, ADDM), apparent digestibility of crude protein (%, ADCP) and the apparent digestibility of the crude lipid (%, ADCL) in the nutrition of tilapia (Oreochromis niloticus). Material and methods. The DW and WH were used to formulate nine isoproteic (32.47%), isolipidic (5.46%) and isoenergetic (16.45 MJ kg-1) diets, with three replicates and 15 male tilapia (36.36± 1.16 g) per repetition: 0% (T0); 10% (T10); 15% (T15); 20% (T20) and 25% (T25), respectively random. A principal component analysis (PCA) was applied. Results. The crude protein (%) of the WH and the DW, were 21.72% and 27.37% (p˂0.05), respectively. Fish fed with WH and DW, up to 10 and 15% inclusion in the diet, respectively, did not show significant differences (p>0.05) for ADDM. However, for ADCP and ADCL, up to 15 and 20%, respectively, significant differences (p>0.05) were not observed. Conclusions. Water hyacinth and duckweed meals were an alternative vegetable protein source, which can partially replace soybean meal and cornmeal in tilapia diets. The PCA suggests that the control diet, 10% WH, 10-15% DW, showed the best results in terms of the apparent digestibility of the nutrients.

Densification of Genetic Map and Stable Quantitative Trait Locus Analysis for Amino Acid Content of Seed in Soybean (Glycine max L.).

Soybean, a primary vegetable protein source, boasts favorable amino acid profiles; however, its composition still falls short of meeting human nutritional demands. The soybean amino acid content is a quantitative trait controlled by multiple genes. In this study, an F2 population of 186 individual plants derived from the cross between ChangJiangChun2 and JiYu166 served as the mapping population. Based on the previously published genetic map of our lab, we increased the density of the genetic map and constructed a new genetic map containing 518 SSR (simple sequence repeats) markers and 64 InDel (insertion-deletion) markers, with an average distance of 5.27 cm and a total length of 2881.2 cm. The content of eight essential amino acids was evaluated in the F2:5, F2:6, and BLUP (best linear unbiased prediction). A total of 52 QTLs (quantitative trait loci) were identified, and 13 QTL clusters were identified, among which loci02.1 and loci11.1 emerged as stable QTL clusters, exploring candidate genes within these regions. Through GO enrichment and gene annotation, 16 candidate genes associated with soybean essential amino acid content were predicted. This study would lay the foundation for elucidating the regulatory mechanisms of essential amino acid content and contribute to germplasm innovation in soybeans.

Potassium Application Impacts Chickpea Yield and Nutritional Status

ABSTRACT As one of the three primary plant nutrients, plant growth and development depend on soil potassium (K) availability. However, plants often face K deficiency in the soil. Chickpea (Cicer arietinum L.) is an excellent source of vegetable protein. However, optimum chickpea growth is affected by K limitation. This study evaluated whether K application in an Oxisol influences chickpea growth, grain yield, and nutritional status. We conducted a greenhouse experiment in an Oxisol with four K application rates (0, 40, 80, and 150 kg ha−1) and four replicates. Our results showed that K application significantly increased chickpea grain yield (R2 = 0.968), shoot weight, root dry weight, and the number of pods. In addition, the K application rate in the soil increased with increasing K application rate. In addition, the concentrations of K and P in the grain are affected by K application. In addition, K improved the K concentration in the shoot (R2 = 0.756) and grain (R2 = 0.833). However, the application of high levels of K did not increase K accumulation in the seeds, indicating that the use of low doses of K (≤80 kg ha−1) is sufficient to support plant growth and nutrition and avoid K loss. Finally, the benefits of K in plants are more than an increase in the grain yield of crops. However, K plays a dominant role in the root system. For instance, this benefit enhances K acquisition from the soil for plants.

Soybean Bioactive Peptide Supplementation Affects the Intestinal Immune Antioxidant Function, Microbial Diversity, and Reproductive Organ Development in Roosters.

Soybean is an important source of high-quality vegetable protein with various health-improving properties, and its main bioactive substances are small peptides produced by in vitro enzymatic hydrolytic processes. In traditional layer breeding, the nutritional health of roosters is frequently neglected, ultimately affecting the quality and quantity of offspring. This study investigated the effects of various quantities (0%, 0.15%, 0.30%, 0.45%, and 0.60%) of soybean bioactive peptide (SBP) feed additives on immunological and antioxidant functions, gut health, and reproductive performance of roosters. SBP supplementation significantly improved male growth and reproductive performance, including growth rate, feed conversion ratio, reproductive organ development, and semen quality. SBP also increased immune and antioxidant levels, boosted the integrity of the small intestinal physiological structure and barrier function, and diversity of cecal microbes, and decreased the apoptotic ratio of small intestinal epithelial cells. The effects of SBP on various functions of males showed a quadratic trend, with the optimal concentration determined to be 0.45%.

Research advances on the hard seededness trait of soybean and the underlying regulatory mechanisms.

Soybean is one of the world's most economically significant crops and is widely utilized as an essential source of vegetable protein and edible oil. Cultivated soybean is domesticated from its annual counterpart, wild soybean, which is considered valuable germplasm for soybean breeding. However, wild soybean accessions generally produce seeds with impermeable coats, a trait known as hard seededness (HS), which is beneficial for long-term seed survival but is undesirable for the uniform water absorption and germination of seeds, thus limiting the utilization of wild soybeans in breeding. In addition, moderate HS can isolate the embryo from the surrounding environment and is thus beneficial for long-term seed storage and germplasm preservation. The HS trait is primarily associated with the structure and chemical composition of the seed coat. Moreover, its development is also influenced by various environmental conditions, such as water and temperature. Genetic analysis has revealed that HS of soybean is a complex quantitative trait controlled by multiple genes or minor quantitative trait loci (QTL), with many QTLs and several causal genes currently identified. Investigating the physiological and molecular mechanisms underlying this trait is crucial for soybean breeding, production, and food processing. For this article, the literature was reviewed and condensed to create a well-rounded picture of the current understanding of internal and external factors, QTLs, causal genes, and the regulatory mechanisms related to the HS of soybean, with the aim of providing reference for future research and utilization of this trait.

Potential of Streptomyces spp. From Peanut Plant Soil as an Entomopathogen of Pests Spodoptera Litura

Peanuts are agricultural commodities that have high economic value and play an important role in meeting human needs, namely as a source of vegetable protein, oil and other nutrients, but domestic peanut production has not been able to meet the needs of the Indonesian people. The national demand for peanuts reaches 856.1 thousand tons per year, and the average consumption of peeled peanuts is 0.32 kg per capita every year. [1] In 2013 peanut production was 701,680 tons, then there was a decrease in production in 2014 to 638,896 tons, and continued until 2015 to 605,449 tons, thus showing a 13.7% decrease that occurred from 2013 to 2015. Low productivity is caused by various factors, one of which is the attack of pests and plant diseases. This study aims to determine the type of Streptomyces spp. Microbes with high chitinolytic content that can control armyworm pests (S.litura) on peanut plants. and influence of biological control agency Streptomyces spp as an entomopathogen of insect pests S. litura. This study was able to improve understanding of the influence of the biological control agency Streptomyces spp as an entomopathogen of S. litura insect pests to protect plants in the future. The results of the exploration of Streptomyces sp. acquired Colonies are irregular and branched coccus-shaped. The colony is tightly attached to the GNA medium, wavy white with a notched surface and a starchy texture. In addition, it has the largest chitinase enzyme content with a chitin zone diameter of 3 cm. PCR test Based on BLAST® result tabulation data that isolates belong to the genus Streptomyces sp. with an identity percentage of 99.86% with Streptomyces sp. VEL gene 17 to 16 rRNA, partial sequence with access code AB914463.2 The highest mortality was in the treatment (S0 RP K3) and (S1 RP K3) there was 60% this showed that with the administration of Streptomyces sp. With a concentration of 75% can cause very high mortality with abdominal contact poison in S. Litura pests

Influence of Soil Additives (Gypsum and Dolomite) on Peanuts Productivity and Soil Properties: A Review

Although India is steadily progressing in oilseeds production, but the average yields of most of the oilseeds like groundnut are still extremely low when compared to those prevailing in other countries of the world. This is because oilseeds are cultivated mostly in marginal and sub-marginal land of semi-arid areas with very less management, hence remain vulnerable to vagaries of nature. Among oilseed crops groundnut is an important source of vegetable oil and protein. Lack of appropriate management practices leads to decreased yields of the crop. Applications of amendments like gypsum and dolomite based on soil pH is necessary to provide a better environment for roots and plant growth.

The Role of Legumes in the Sustainable Mediterranean Diet: Analysis of the Consumption of Legumes in the Mediterranean Population over the Last Ten Years a PRISMA Statement Methodology

The Mediterranean model, introduced by Ancel Keys, has gained significant importance from the perspective of sustainability. This is why the Mediterranean diet is now called a sustainable diet. This study focuses on legumes, protein-rich foods of vegetable origin, cultivated in the area, as their production and use in cooking are highly sustainable. The analysis covers their consumption over the last 10 years in the entire Mediterranean area, looking specifically at Spain. The objectives of this work are to analyze the production of legumes in the Mediterranean area, taking into account the fact that they are an important environmental as well as food resource, and to analyze how the consumption of legumes has changed in certain countries bordering the Mediterranean in relation to social and cultural changes, with particular attention to the pandemic. The study shows how, in Spain, since 2020, the consumption of legumes has increased, demonstrating that in emergency situations, different dietary choices are made. In addition, it shows how legumes are an excellent source of vegetable protein and an important environmental resource from the perspective of sustainability.

Black Soybean Tempeh Ice Cream (Glycine Max Var. Mallika) Rich in Antioxidants to Increase Endurance

Low fat intake can contribute to preventing and reducing the risk of degenerative diseases and antioxidants can prevent oxidative stress and increase the body's endurance. Soybeans are a source of low-fat vegetable protein and contain isoflavones which have the potential to act as anti-inflammatory agents. Butterfly pea flowers are one of the plants that are used as a source of antioxidants. This research aims to develop ice cream that is low in fat and contains antioxidants by using black soybean tempeh milk and butterfly pea flower coloring. This research stage includes formula creation, sensory testing, analysis of physical characteristics, and analysis of antioxidant capacity. This research used three treatments with different dilution ratios of black soybean tempeh and water, namely P1 (15% dilution), P2 (20% dilution), P3 (35% dilution). Based on the results of the sensory test, P3 was the selected formula. The antioxidant capacity of black soybean tempeh milk ice cream with the addition of butterfly pea flower coloring is stated in IC50 at 6816.66 ppm or the equivalent of 13.56 mg Vitamin C/100 g. One serving of soy milk ice cream in this study can fulfill the low-fat claim and antioxidant.

Feed production for sustainable aquaculture: A Bibliometric Network Analysis

In recent years, aquaculture has played a fundamental role in human nutrition and livestock for the ability to produce different animal and vegetable protein and lipid sources essential for human and animal health. However, the growing increase in aquaculture has led to a greater demand for ingredients composing feed for aquatic organisms, many of which are derived from wild fish making the aquatic feed production an unsustainable process. For this reason, the aquaculture sector has expanded experimentation to search for alternative ingredients to reduce environmental impact. This study explored the global scientific literature on sustainable aquaculture with particular reference to feeding using VOSviewer software, which allows for generating, visualizing, and exploring maps based on bibliometric network data. The results allowed a comprehensive overview of the scientific literature on sustainable aquaculture through network maps displaying the relationships among keywords, authors, countries, and journals. In detail, this paper highlighted that in the last years, particularly in recent decades, the research widely focused attention on different aspects of the sustainable aquaculture field. The first journal that researched sustainable aquaculture was the Aquaculture Journal, and the leading countries that pursued this type of research were the USA, UK, and China. Concerning the co-occurrence, the top keywords were aquaculture, sustainability, animals, nonhuman, and sustainable development, highlighting a growing interest in research on microalgae, diet, fishmeal, and climate change. The description of the current state of the art in sustainable aquaculture reported in this article highlighted that the combined use of social network analysis and bibliometrics allows exploring the development of research in specific fields of science and lays the foundations for delving into questions that have not yet been sufficiently investigated.

Technological and textural properties of gluten-free quinoa-based pasta (Chenopodium quinoa Wild)

Quinoa (Chenopodium quinoa Willd.) is an Andean grain with a perfect nutritional composition that, by diversifying its transformation, becomes an attractive alternative for consumers looking for a high-quality, healthy diet with a source of vegetable proteins. The objective of this work was to elaborate and evaluate the technological and textural properties of quinoa paste in its entirety through the Star-Shaped Composite Central Design (CCD) of 5 process variables: Water Temperature (°C), Water Quality (ml), Mixing Time (min), Drying Temperature (°C), and Drying Time (min), with 5 levels each. At the same time, the yield and good cooking quality were studied to optimize the process. In the model of the equation for the cooking time response, a negative and significant influence of drying temperature was shown. On the other hand, for cooking loss, dough gain, a* and b* values, and texture had high values if the drying time was increased. On the contrary, the L-value decreases, which is positively significant. Meanwhile, the swelling index was only significantly positive within the technological properties. In addition, it was found that the optimal conditions for producing quality pasta were 25 °C: 1150 ml: 30 min: 70 °C and 80 min, respectively, with a desirability of 0.883. When the pasta was prepared with quinoa, the cooking time was 7 min, the cooking loss was 2.46 g/g, the mass gain was 23.6 g/g, the cooking yield was 7.99%, the swelling index was 2.9%, water absorption was 135%, and protein was 12.71 g and 0. 21 Pa in texture, these results being consistent with cited research. Likewise, the whiteness was 51.97 for the values a* 2.41 and b* 12.45; all this analysis is reflected in the final yield of the process at 78%. In conclusion, the results indicated that, by optimizing the conditions in the production of gluten-free quinoa pasta, it is possible to obtain a gluten-free product with high added value, excellent cooking quality, adequate technological properties, texture, and color acceptable to the consumer.

Integrated Transcriptomic and Proteomic Characterization of a Chromosome Segment Substitution Line Reveals the Regulatory Mechanism Controlling the Seed Weight in Soybean

Soybean is the major global source of edible oils and vegetable proteins. Seed size and weight are crucial traits determining the soybean yield. Understanding the molecular regulatory mechanism underlying the seed weight and size is helpful for improving soybean genetic breeding. The molecular regulatory pathways controlling the seed weight and size were investigated in this study. The 100-seed weight, seed length, seed width, and seed weight per plant of a chromosome segment substitution line (CSSL) R217 increased compared with those of its recurrent parent ‘Suinong14’ (SN14). Transcriptomic and proteomic analyses of R217 and SN14 were performed at the seed developmental stages S15 and S20. In total, 2643 differentially expressed genes (DEGs) and 208 differentially accumulated proteins (DAPs) were detected at S15, and 1943 DEGs and 1248 DAPs were detected at S20. Furthermore, integrated transcriptomic and proteomic analyses revealed that mitogen-activated protein kinase signaling and cell wall biosynthesis and modification were potential pathways associated with seed weight and size control. Finally, 59 candidate genes that might control seed weight and size were identified. Among them, 25 genes were located on the substituted segments of R217. Two critical pathways controlling seed weight were uncovered in our work. These findings provided new insights into the seed weight-related regulatory network in soybean.