Vegetable Protein Diet Research Articles

The Effect of Nutritional Status and Diet on Hipertension Disesase in Tabaringan Health Care

Hypertension is a disease that is defined as a persistent increase in blood pressure. According to WHO, the increase in hypertension is mainly driven by several factors the main risks are unhealthy eating and living patterns, lack of physical activity, etc. For example, the diet that is thought to be the main cause contains high energy, high carbohydrates, high fat, high cholesterol, high sugar, high salt, instant food and smoking habits. This can also cause fat accumulation which over time will result in weight gain and manifest in hypertension. To determine the effect of nutritional status and diet on the incidence of hypertension at the Tabaringan Community Health Center In this research, the case control method was used with the type of analytical observational research. Data collection techniques use primary data. The collected data was analyzed using the Chi-Square Test. Respondents were taken using techniques consecutive sampling. Respondents were 64 people consisting of 32 people each with hypertension and non-hypertension groups. The results of the study showed that there was a significant relationship between nutritional status and hypertension p=0.000, animal protein diet to hypertension p=0.000, vegetable protein diet to hypertension p=0.000, vegetables diet to hypertension p=0.000, fruits diet on hypertension p=0.000. However, there was no significant relationship between carbohydrate diet and hypertension, p=0.217. There is a significant relationship between overweight/obesity nutritional status and a diet of animal protein, vegetable protein, fruits and vegetables on the incidence of hypertension at the Tabaringan Community Health Center, Makassar City.

Effect of vegetarian diets on renal function in patients with chronic kidney disease under non-dialysis treatment: A scoping review.

Vegetable protein diets (VPDs) in chronic kidney disease (CKD) patients may be related to beneficial biological actions and possibly clinical impact. This is a scoping review that merge studies that evaluated the effect of a vegetarian diet on kidney function in adults with CKD under non-dialysis treatment. The evaluated outcome was the impact in renal function assessed by eGFR or creatinine clearance. MEDLINE (accessed by PubMed) was searched up to September 8, 2020. Data were extracted by two independent reviewers, who also assessed the quality of the studies. Of 341 retrieved articles, 4 studies assessing 324 patients were included in the analysis. One study showed that a very low-protein ketoanalogue-supplemented vegetarian diet had benefits in relation to a conventional low-protein diet, while the other three studies demonstrated no difference in kidney function between the evaluated diets. Additional studies are needed to assess the benefits of vegetarian diets for further recommendations in CKD management.

Effect of Resistance Training and Diet Intake on Spleen Structure of Ovariectomized Wistar Rats

Abstract Introduction Exercise is a key factor in immunity which may be affected by a different diet intake. Thus, we aimed to analyze the effects of diet intake and resistance training on spleen structure of ovariectomized female animals. Materials and Methods Female Wistar rats were divided into eight experimental groups: sedentary and nonovariectomized animals plus vegetable protein diet (CVS) or animal protein diet (CAS); trained and nonovariectomized rats plus vegetable protein diet (CVT) or animal protein diet (CT); sedentary and ovariectomized groups plus vegetable protein diet (VOS) or animal protein diet (AOS); and trained and ovariectomized animals plus vegetable protein diet (VOT) or animal protein diet (AOT). Results Groups submitted to both animal protein diet and resistance training, mainly ovariectomized groups, presented a great variability of collagen fibers type III, white pulp, and follicle structure in relation to the other spleen constituents. Conclusion Resistance training with a vegetable protein diet may play a key factor to maintain spleen’s immune responses across age. However, animal protein diet is suggested to decrease spleen’s immune activity.

Crude Protein Requirements Of Egg-type Baby Chicks Under Nigerian (Tropical) Environment

TWO feeding trials were conducted with day old mixed sexes of Apollo (White Leghorn) strain. The first experiment was designed to test the effectiveness of all — vegetable protein diets to meet the requirements of baby chicks. While the second one was designed to find out whether synthetic methionine and/or fishmeal supplementation are desirable in low quality protein of groundnut cake. Results showed that a diet based on plant proteins only, namely, those of groundnut cake and maize, is capable of stimulating rapid growth in chicks. Diets containing 24% all-vegetable protein, 22% plant protein plus 0.35% feed grade methionine or 20% plant protein containing 3% of fishmeal plus 0.37% feed grade methionine, satisfied the requirements for rapid growth and efficient feed utilization in young chicks. However, based on the prevailing prices of the ingredients used in formulating the rations, the results indicate that ration based on 24%of all — plant protein diet was least expensive.

Effects of different diet intake and resistance training on left ventricle remodeling in ovariectomized rats

It is known that physical activity promotes positive physiological adaptations, especially in cardiovascular responses in individuals of different age groups; however, it is necessary to know if different diet influences the effects of exercise on the left ventricular remodeling. Its aim is to evaluate the effects of resistance training associated with vegetable and animal protein diets on the left ventricular remodeling of ovariectomized aged animals. Female Wistar rats were divided into eight experimental groups: sedentary and non-ovariectomized animals plus vegetable protein diet (CVS) or animal protein diet (CAS); trained and non-ovariectomized rats plus vegetable protein diet (CVT) or animal protein diet (CAT); sedentary and ovariectomized groups plus vegetable protein diet (VOS) or animal protein diet (AOS); trained and ovariectomized animals plus vegetable protein diet (VOT) or animal protein diet (AOT). Ovariectomy promoted an increase in the density of collagen type I and III in all animals, being more marked in the groups of vegetal protein diet (p < 0.05). Additionally, our ultrastructural analysis demonstrated a greater amount of type I collagen fibers and a decrease of type III collagen fibers in the sedentary ovariectomized animals regardless of the diet consumed. We can observe that VOT and AOT had a significant decrease (p < 0.05) of MMP-2 when compared to the other groups. In addition, both training and/or ovariectomized groups demonstrated an increase in MMP-9. We observed the benefits of resistance training associated mainly with the animal diet on cardiac tissue, highlighting the importance of these interventions in the prevention and reversal of changes in the various organs and systems resulting from menopause.

Replacing fish meal with vegetable protein sources in feed for juvenile red swamp crayfish, Procambarus clarkii : Effects of amino acids supplementation on growth and feed utilization

An 8-week growth trial was conducted to investigate the effects of dietary fish meal replacement with a vegetable mixture of soybean meal and rapeseed meal (1:1) on growth of juvenile red swamp crayfish. Nine isonitrogenous diets were designed: V0, V34, V50, V65, V73 and V81 with six levels of vegetable proteins, and VA48, VA63 and VA78 by further adding crystalline lysine and methionine into V50, V65 and V81. Compared with V0, V34 significantly improved the specific growth rate (SGR), while V65, V73, V81 and VA78 depressed the SGR (p < .05). Feeding rate showed a decreasing trend as dietary vegetable protein level increased (p < .05), except that in VA48 group. Significantly lower FCR and higher PER were observed in V34 group, whereas all vegetable protein diets depressed the feed utilization of crayfish (p < .05). Crayfish fed with diets containing vegetable proteins showed significantly lower hepatosomatic indices and higher condition factors than the control (p < .05). Muscle lipid content was significantly (p < .05) lowered in V81 group, but not in VA78 group. The results suggested that 338 g/kg vegetable protein improved growth performance of crayfish. Excessive vegetable protein depressed the growth of crayfish, which could be prevented by lysine and methionine supplementation except for the all vegetable protein diets.

Value added by Spirulina platensis in two different diets on growth performance, gut microbiota, and meat quality of Japanese quails.

Aim:The growth promoting effect of the blue-green filamentous alga Spirulina platensis (SP) was observed on meat type Japanese quail with antibiotic growth promoter alternative and immune enhancing power.Materials and Methods:This study was conducted on 180 Japanese quail chicks for 4 weeks to find out the effect of diet type (vegetarian protein diet [VPD] and fish meal protein diet [FMPD])- Spirulina dose interaction (1 or 2 g/kg diet) on growth performance, gut microbiota, and sensory meat quality of growing Japanese quails (1-5 weeks old).Results:Data revealed improvement (p<0.05) of weight gain, feed conversion ratio and European efficiency index due to 1, 2 g (SP)/kg VPD, and 2 g (SP)/kg FMPD, respectively. There was a significant decrease of ileum mean pH value by 1 g (SP)/kg VPD. Concerning gut microbiota, there was a trend toward an increase in Lactobacilli count in both 1; 2 g (SP)/kg VPD and 2 g (SP)/kg FMPD. It was concluded that 1 or 2 g (SP)/kg vegetarian diet may enhance parameters of performance without obvious effect on both meat quality and gut microbiota. Moreover, 1 and/or 2 g (SP) may not be invited to share fish meal based diet for growing Japanese quails.Conclusion:Using of SP will support the profitable production of Japanese quails fed vegetable protein diet.

Effects of marine protein‐, marine oil‐ and marine‐free diets on the growth performance and innate immune responses of Atlantic salmon ( Salmo salar , L.) post‐smolts

Atlantic salmon post-smolts of an average of 940 g were fed six diets including two marine-based commercial diets one with partial inclusion of vegetable proteins (VPs) and oils (VOs) (2011/12 EU standards) (MB) and a second with partial inclusion of VPs, land animal-by-product (ABP) proteins and VOs (non-EU standards) (MBABP), a fully vegetable protein (VP) diet; a fully algal and VOs (VO) diet; a fishery-free vegetable-based (VP/VO) diet; and a fishery-free diet with a mix of VPs and ABP proteins and a mix of algal and vegetable oils (MFABP). Growth was assessed at Days 104 and 175, whereas fillet proximate composition, haematology and innate immune responses were assessed upon termination. Overall, MB salmon was the best performing group for the full period in terms of feed intake and overall weight gain. MB and VP salmon exhibited the highest FCRs compared to the other groups, while VP salmon exhibited the highest condition factor (K) and VO salmon the lowest K compared to the other groups. Fillet proximate composition did not present differences among the six groups. MB salmon demonstrated the highest plasma lysozyme activity compared to the other groups while MFABP, VP and VP/VO salmon demonstrated higher plasma anti-protease activity in contrast to MB salmon. The dietary groups did not present differences in plasma protein, total IgM or natural haemolytic activity while unaltered head kidney macrophage respiratory burst activity was also observed. Overall, diets free from marine proteins or oils and/or both were satisfactorily utilized by salmon without compromising their immune capacity, although longer adaptation periods are required.

High Vegetable Protein Diet is Associated with Low Lumbar Spine Bone Mineral Density in Amenorrheic Athletes

PURPOSE: We have reported that amenorrheic athletes (AA) have a higher intake of dietary fiber compared to eumenorrheic athletes (EA) and non-athletes (NA), which is negatively associated with lumbar BMD. High fiber diets are rich in vegetable proteins, and food rich in vegetable protein has high concentrations of phytic acid and isoflavones, and low concentrations of saturated fatty acids (SFA). Fiber and isoflavones are reported to have negative associations with serum estrogen. We hypothesized that a diet high in vegetable protein may contribute to low BMD in AA. METHODS: 4-day food records and serum samples were collected from 68 AA, 24 EA, and 26 NA 14–23 yo. Energy availability was calculated as (energy intake- energy expenditure)/ fat free mass. DXA was used to assess lumbar spine BMD. RESULTS: EA were younger than AA and NA (p=.04), and AA had lower BMI (p=.04). Exercise activity did not differ in AA and EA. There was no difference in energy availability among groups. Compared with EA and NA, AA had higher intake of fiber (AA=34.8±15.3; EA=22.5±11.7; NA=17.2±6.6g; p<.0001), phytic acid (AA=1276±621; EA=621±384; NA=700±370mg; p<.0001), and vegetable protein (AA=45.6±19.2; EA=31.9±12.9; NA=26.8±8.8g; p<.0001). Intake of isoflavones was higher in AA than NA (genistein: AA=7.4±10.1; EA=2.7±6.7; NA=1.4±4.2g; p=.003; daidzein: AA=5.9±8.0; EA=2.0±4.8; NA=1.0±2.9g; p=.0002). AA had a lower intake of % calories from SFA than NA (AA=8.6±2.6; EA=9.8±2.2; NA=10.6±2.6; p=.002). After controlling for menstrual status, calcium intake and serum vitamin D, spine Z-scores were associated inversely with vegetable protein (SE=−.34, p=.006), phytic acid (SE=−.32, p=.005), genistein (SE=−.29, p=.003), and daidzein (SE=−.28, p=.005) ; and positively with %SFA after also controlling for fiber (SE=.26,p=.02). CONCLUSIONS: We found a higher vegetable protein diet in AA than EA and C, associated inversely with spine Z-scores. Because phytic acid and dietary fiber decrease energy and mineral absorption, some AA may have adequate energy intake but be nutrient deficient. Furthermore, the ability of dietary fiber and isoflavones to decrease estrogen may affect BMD. Studies examining nutrients affecting bone metabolism and estrogen levels in female athletes are needed to develop optimal dietary recommendations.

Effect of production phase on growth, enzyme activities and feed selection of broilers raised on vegetable protein diet.

This study consisted of two experiments, conducted to assess the impact of phase at which vegetable protein (VP) diets are introduced to broiler chicks, and preference of birds for diets based on soybean or canola meal (CM). Two hundred and ten day-old Cobb 500 chicks were randomly distributed into five dietary groups in the main experiment. One group was fed on animal protein (AP) diet all through to 21 days of age; two other groups were started on AP diet for 7 days and then switched to diets containing soybean meal (AP-SBM) or AP-CM, while two other diets (SBM-AP and CM-AP) were started on one of the VP diets for 7 days and then switched to AP diet. A sub-experiment on thirty birds raised on a commercial diet to 7 days was used in a feed selection test to quantify the preference of birds for the diets containing mainly CM or SBM. Chicks were reared under similar care and management conditions and the diets were iso-caloric and iso-nitrogenous. Results of the main experiment showed that chicks on CM-AP diet ate more (p<0.05) than those on the other diets up to day 7. Body weight gain was highest (p<0.001) on the AP-SBM diet while birds on the CM-AP diet weighed the least at 7 d. Feed intake, body weight gain, feed conversion ratio, mortality, bone growth, visceral organ development, and activities of digestive enzymes were similar between the groups from hatch to 21 days of age. Results of the second sub-experiment showed that chicks preferred the CM-based diets to the SBM-based diets at 8 to 14 d (p<0.001) and 15 to 21 d (p<0.01) when given a choice. Overall, the birds were not affected by the nature of the starter diet although they tended to prefer the canola to soybean diets.

Impact of Microbial Enzymes on Growth Performance, Micronutrient Digestibility, Tissue Protein Contents and Endogenous Enzyme Activities of Broiler Chickens Fed on Vegetable Protein Diets

A study was conducted to investigate the effects of dietary protein sources and microbial enzyme supplementation on amino acid and mineral digestibility, tissue protein contents and endogenous enzyme activities of broiler chickens. Ross 308 day-old male broiler chicks (n = 160) were assigned randomly to four dietary treatments, each replicated five times, eight chicks per replicate, in a 2 x 2 factorial design. Two basal diets were formulated with soybean (SBM) and canola (CM) meals at a ratio of 75:25, respectively, along with basal grains and fed to the birds as such or supplemented with enzymes from 1 to 35 days. Enzymes had a positive effect on the gross responses (feed intake, body weight and feed conversion ratio) of the broiler chickens at 35 days. However, feed intake was significantly different (p 0.05) body weight and feed conversion ratio (FCR) between 1 and 35 days. Tissue protein contents and endogenous enzyme activities at 21 days (except for maltase) were unaffected by dietary sources and microbial enzyme supplementation. The activity of maltase was higher (p 0.05) on mineral digestibility of broilers over the test period. The digestibility of Cu, Zn and Mg was increased (p<0.05) on CM diet, whereas Ca digestibility was higher on SBM diet at 35 days. It can be concluded that the improved growth of the birds might be a result of increased digestibility of amino acids and increase in feed consumption.

Influence of Dietary Amino Acid Profile on Serum Lipids in Hypercholesterolemic Chinese Adults

To investigate the significance of Lys:Arg and Met:Gly ratio on serum lipids concentrations and adiponectin in hypercholesterolemic adults, 24 g of soy isolate protein supplement (SIP) or milk protein supplement (CON) were randomly given to 90 subjects (n=45/group) daily for 8 weeks. The correlation between dietary proteins, Lys :Arg and Met:Gly ratio and the percentage changes in lipids and adiponectin in 90 subjects were examined. The percentage changes in total and LDL cholesterol concentrations were significantly positively correlated with the ratio of dietary vegetable protein/animal protein (VP/AP) (rs=-0.595, P<0.001 and rs=-0.606, P<0.001 respectively). The changes of total and LDL cholesterol concentrations were significantly correlated with dietary Lys:Arg ratio (rs=0.574, P<0.001 and rs=0.582, P<0.001 respectively). The change in adiponectin was negatively correlated with dietary Lys:Arg and Met:Gly ratio (rs=-0.668, P<0.001 and rs=-0.309, P=0.003 respectively) and was positively correlated with the ratio of VP/AP (rs=0.566, P<0.001). Vegetable protein diet with low Lys :Arg ratio may have a favorable effect on serum total cholesterol, LDL cholesterol and adiponectin.

Growth responses, excreta quality, nutrient digestibility, bone development and meat yield traits of broiler chickens fed vegetable or animal protein diets

This study was carried out to compare the performance of broilers fed diets containing only vegetable protein (VP) with birds that received an animal protein (AP) in their diets. Cobb 500 day-old male broiler chicks (n = 256) were randomly divided into four experimental groups. The two AP diets contained fish meal and soybean (SBF) and fish meal with canola (CMF); while the two VP diets contained predominantly soybean (SBM) or canola (CAN) meals. All diets were isoenergetic and isonitrogenous, and were pelleted, but amino acid levels were formulated on a total and not digestible basis. Feed intake up to 21 days was highest on the AP diets, and the lowest in the SBM treatment. Birds in the AP diet groups were significantly heavier at 21 days and 35 days than those on the VP diets. Up to 35 days, birds on AP diets had superior feed conversion ratio, while the CAN treatment was the poorest. Excreta moisture level was significantly higher in birds fed the VP diets than those on AP diets. Excreta pH and ammonia concentration were similar between treatments. Protein digestibility was higher in the AP than in the VP diets. Birds raised on VP diets had a significantly lower abdominal fat content than birds on the AP diets. Other meat characteristics measured in this experiment did not differ significantly. Bone development, in terms of breaking strength and latency-to-sit time, was significantly better on the AP diets than that of birds on the VP diets. The birds on the CMF diet had the longest tibia bone, while birds on SBM diet, the shortest. Total tibia ash content on the CMF diet was significantly increased, along with its iron and copper concentration, which were also significantly higher in birds on the same diet than the others. The responses of birds generally indicated that the AP diets were superior to the VP diets.Keywords: Growth, digestibility, fish meal, bone growth, meat quality, soybean meal, canola meal

Effects of different protein and glycemic index diets on metabolic profiles and substrate partitioning in lean healthy males

Dietary glycemic index (GI) and protein affects postprandial insulin responses and consequently 24 h glucose metabolism and therefore substrate partitioning. This study investigated the mechanistic effects of different protein and GI diets on 24 h profiles of metabolic markers and substrate partitioning. After 3 days of diet and physical activity standardization, 10 healthy male subjects (BMI: 22.5 ± 0.6 kg/m(2)) stayed in a respiration chamber 4 times for 36 h each time to measure substrate partitioning. All subjects randomly received four isoenergetic diets: a normal (15En%) dairy protein and low GI (<40 units) (NDP-LGI) diet; a high (25En%) dairy protein and low GI (HDP-LGI) diet; a normal vegetable protein and low GI (NVP-LGI) diet; or a normal dairy protein and high GI (>60 units) (NDP-HGI) diet. During the day, blood was sampled at fixed time points for the measurement of metabolic markers and satiety hormones. The HDP-LGI diet increased 24 h protein oxidation and sleeping metabolic rate (SMR) compared with the NDP-LGI diet (p < 0.002). No significant differences in 24 h carbohydrate and fat oxidation (day and night) were found between all intervention diets. Net incremental area under the curve (net iAUC) of 24 h plasma glucose decreased in the HDP-LGI diet compared with the NDP-LGI diet (p < 0.01), but no effect was observed on insulin levels. No difference in appetite profiles were observed between all intervention diets. The lower 24 h glycemic profile as a result of a high dairy protein diet did not lead to changes in 24 h substrate partitioning in lean healthy subjects with a normal insulin sensitivity.

Can the incidence and mortality of chronic diseases be explained by dietary patterns?

Can the incidence and mortality of chronic diseases be explained by dietary patterns?

Upgrading the nutritive value of full-fat soyabeans meal for broiler production with either fishmeal or black soldier fly larvae meal (&lt;i&gt;Hermetia illucens&lt;/i&gt;)

An experiment was conducted using Anak Red broiler chicks to evaluate the effect of grading the nutritive value of full fat soyabean meal diet based with either fishmeal or Blaick soldier fly larvae meal for broiler production. The average live weight gains of broilers fed an all vegetable protein, fishmeal and larvae meal diets at 10 weeks of age were 1465g, 2045g and 2015g respectively. The corresponding feed consumption values for same period were 4140g, 4878g and 5075g while the figures for the feed/gain ratio were 2.8 2.4 and 2.6 respectively. Significant (P&lt;0.05) treatment effects in favour of diet upgraded with the larvae meal were obtained in the carcass yield, internal organ measurements (kidney, gizzard and liver) and the abdominal fat. The amino acid profile of the larvae meal compared favourably with that of fishmeal and also found to be superior to that of soybean meal. The study suggests that inclusion of protein of animal or insect origin to an all-vegetable diet was very desirable for better nutrient utilization. The results also revealed that under the conditions of the experiment, larvae meal could replace fish meal to upgrade the nutritive value of soyabean meal in the broiler starter and finisher rations without any adverse effect on the rate of gain, feed consumption and feed: gain ratio. Key words: Black soldier fly, larvae meal, soybean meal, fish meal, broiler.

Intestinal metabolism of weaned piglets fed a typical United States or European diet with or without supplementation of tributyrin and lactitol

The aim of the study was to investigate the effects of supplementation of a microencapsulated blend of tributyrin and lactitol (TL) to a standard European (EU) diet without antibiotic growth promoters on intestinal metabolism and mucosa development of weaned piglets and to compare it with a standard US diet containing animal proteins, zinc oxide, copper sulfate, and carbadox. Ninety piglets weaned at 21 d were divided into 3 dietary groups consisting of 5 replicates each: 1) US diet supplemented with 55 mg/kg of carbadox, and 2.5% each of plasma proteins and spray-dried blood cells in the first phase, 3,055 mg/kg of Zn in the first and second phases, and 180 mg/kg of Cu in the third phase; 2) EU diet based on vegetable proteins and no antibiotics; and 3) the same EU diet supplemented with 3,000 mg/kg of microencapsulated TL. The study was divided into 3 phases: 0 to 7, 8 to 21, and 22 to 35 d. On d 7, 21, and 35, animals were weighed, and feed consumption and efficiency were determined. On d 14 and 35, one pig per pen was killed, and the intestinal contents and mucosa from the proximal, middle, distal jejunum and the ileum were sampled. Intestinal wall sections were fixed for histological analysis, and intestinal content was used for VFA, ammonia, and polyamine analysis. Throughout the study (d 0 to 35), the US diet had greater ADG and ADFI than the EU diet (P < 0.05). The EU diet supplemented with TL tended to have 11% greater ADG (P = 0.17). Feeding the EU diet caused a reduction in proximal and middle jejunum villi length by 10% (P < 0.05) and an increase in crypt size in proximal jejunum (P < 0.05) compared with the US diet, probably due to an increased rate of cell loss and crypt cell production. The TL supplementation resulted in longer villi along the jejunum and less deep crypts in the proximal jejunum (+15.9 and -8.9%, respectively; P < 0.05) than the unsupplemented EU diet. The TL diet increased the concentrations of cadaverine and putrescine in the small intestine (P < 0.05) and seemed to increase cadaverine, histamine, putrescine, and spermine in the large intestine by 1.5- to 10-fold compared with the US or EU diet. In conclusion, although the US diet had a greater effect on growth performance and mucosal trophic status than the EU diets, the supplementation with slowly released TL seemed to be an effective tool to partially overcome the adverse effects of vegetable protein diets.

Welfare and quality of farmed trout fed high plant protein diets. 1 Growth performance and quality traits

This study was performed to evaluate the effects of high levels of inclusion of plant protein sources to replace fish meal in rainbow trout (O. mykiss) diets on growth performance and quality characteristics. Two isoproteic (44.8%) and isolipidic (19.6%) extruded diets were fed to eight groups of trout (IBW 106.6g) for 103 days. Diet FM, containing only fish meal as protein source, was used as control treatment and compared to diet PV80, where a plant protein mix (pea protein concentrate and wheat gluten) was included to replace 80% of fish meal protein. Growth performance (FBW: 318.5g; SGR: 1.06%) and feed to gain ratio (0.79) of the fish were not affected by dietary treatment (p>0.05). Dry matter and protein ADCs, measured in vivo after stripping, resulted higher in fish fed the high vegetable protein diet relative to the fish meal one (p<0.05). Trout fed diet PV80 were characterised by higher agility index (2.02 vs 1.72, p<0.05), carcass yield (91.95 vs 91.18%, p<0.05), lower hepatosomatic index (0.74 vs 1.07%, p<0.05) and viscerosomatic index (8.04 vs 8.82%, p<0.05) but higher mesenteric fat index (3.08 vs 2.88%, p<0.05) relative to FM fed trout. No significant effect was observed in terms of fillet yield (59.28 vs 6.41%, p<0.05). Feeding diet PV80 resulted in a significant reduction of fillet pH 48h post mortem (5.56 vs 6.44, p<0.05) while no reduction was observed in fillet of trout fed the FM diet (6.56, p>0.05). Higher dry matter (28.2 vs 26.8g/100g, p<0.01) and hydroxyproline (0.79 vs 1.31mg/g, p<0.01) content as a measure of total collagen, was observed in fillets of trout fed PV80 diet relative to the control ones. According to these data, also post mortem firmness measured by the Shear force of raw muscle resulted higher in fish fed the plant protein based diet (4.06 vs 3.30N, p<0.05). The firmness reduction observed 48h post mortem (4.24 vs 2.95N, p<0.05) resulted not affected by the dietary treatment.In summary, data show that even if the growth performance of rainbow trout fed a diet in which fish meal was largely replaced by high quality plant proteins resulted similar, some major quality traits such as dressing out, fillet composition and texture can be significantly affected by dietary treatment.

Effect of Practical Diets with Animal or Vegetable Protein Sources and Poultry Oil or Menhaden Fish Oil on Adult Fathead Minnow in Tanks

AbstractThe fathead minnow Pimephales promelas is one of the main species cultured in ponds in the southern United States for bait and is widely used as a toxicological and biomedical model in the laboratory. Diet can have a major impact on performance criteria, both in laboratory and production settings. However, the nutrient requirements of fathead minnow are unknown and cannot be used to formulate optimal diets. To facilitate applied nutrition research for this species, we measured the growth, survival, feed conversion, and response to low dissolved oxygen exhibited by adults that were held in tanks and fed practical diets with animal (menhaden Brevoortia spp. fish meal and poultry by‐product meal) or plant (mostly soybean) protein sources and either poultry oil or menhaden fish oil for 12 weeks. Good weight gain (0.6–0.8 g) was obtained either with vegetable or animal protein sources. Poultry oil enhanced growth relative to menhaden fish oil in diets with either vegetable or animal protein sources. Mean survival rates of fish fed the animal and vegetable protein diets were 88% and 96%, respectively, and survival was not affected by lipid source. Survival during the low‐oxygen stress test was high (≥90%) across diets, and there were no treatment effects. Vegetable proteins and poultry oil are promising alternatives to more costly feed ingredients in practical diets for fathead minnow.

Effect of dietary vegetable and animal proteins on atherothrombosis in mice

Objective It is believed that vegetable and fish, but not animal, proteins prevent thrombosis. The present study compared the effect of long-term intake of purified vegetable and animal proteins (casein, pork, egg white, chicken, white and red fish, soybean, and potato) and powders from whole vegetable and animal meats (soybean, pork, chicken, and horse mackerel) on thrombotic tendency. Methods Western-style high-fat diets with various proteins were given to mice deficient in apolipoprotein-E and low-density lipoprotein receptor for 8 or 12 wk. Thrombotic tendency was assessed by a laser-induced thrombosis test, severity of atherosclerosis was assessed by morphometric analysis of the aortic lesions, and reactivity of platelets was measured by a shear-induced platelet function test. Results There was no difference in thrombotic tendency between the vegetable and animal protein diets with the exception of pork protein, which showed a prothrombotic effect. The latter was not observed in animals kept on a high-protein diet. Thrombotic tendency was not affected by preparation of dietary powders from whole pork, chicken, or horse mackerel meats and casein (control), all of which contained the same percentages of protein and lipid. In contrast, soybean powder showed an antithrombotic effect, which was mainly due to an inhibition of atherogenesis rather than to platelet inhibition. Conclusion Neither the purified animal nor the vegetable protein diet affected thrombotic tendency. Soybean powder inhibited atherothrombosis mainly by inhibiting the atherosclerotic process.