Hydrogels composed of polymer networks are widely used in industry and scientific research for high water retention and unique mechanical properties. Nevertheless, in ordinary hydrogel formation, the trade-off relationship between stiffness and mesh size remains a crucial consideration for practical applications. This study describes a facile approach to controlling hydrogel stiffness and mesh size by hybridizing poly(ethylene glycol) diacrylate (PEGDA) and methacrylated TEMPO-oxidized cellulose nanofibers (T-CNFMA). After disintegrating T-CNF by ultrasonication, T-CNFMA was synthesized resulting in a degree of substitution of 2.04 mmol/g. Incorporation of T-CNFMA in the PEGDA network allowed for independent control of hydrogel stiffness and mesh size by reinforcing the whole hydrogel network as a framework. Consequently, the swelling ratio and shear modulus could be manipulated by controlling the PEGDA/T-CNFMA ratio. Structural analyses revealed that an increase in the T-CNFMA content in the presence of a low amount of PEGDA resulted in a large mesh size with a constant stiffness. The diffusivity test was also consistent with the properties of the hydrogels. This result indicates that the incorporation of T-CNF in hydrogel network is useful to control the physical property of the hydrogel, especially for varying mesh size, regardless of stiffness alteration.