The family Cucurbitaceae includes pumpkin (Cucurbita moschata Duch.), one of the most significant vegetable crops cultivated both in India and overseas. Various biotic and abiotic factors affect pumpkin production, and one of the biggest threats to its cultivation is the cucumber mosaic virus disease. An experiment was conducted in the experimental field of the Department of Plant Pathology, Biswanath College of Agriculture, Assam Agricultural University, Assam during 2022–2023 with the aim of screening out various varieties of pumpkin and identifying the one that was most resistant to cucumber mosaic virus in view of the disease's significance. Out of eighteen pumpkin cultivars evaluated against cucumber mosaic virus disease, six were moderately susceptible, eleven were susceptible and one cultivar was observed to be highly resistant against the disease.