Papaya Varieties Research Articles

Comparative Study of the Agronomic and Organoleptic Performances of Some Varieties of Papaya (Carica papaya L.) Grown Outdoors and in Polyhouse in Kerala (India)

The present study was undertaken with the objective to evaluate the performance of papaya varieties under two growing conditions (open and polyhouse) in terms of yield and quality attributes. The study was conducted in Fruits Crops Research Station, Kerala Agricultural University, Thrissur during 2022-2023. The seedlings of seven papaya varieties, CO-3, CO-7, Arka Surya, Arka Prabhath, Coorg Honey Dew, Pusa Delicious and Red Lady were planted under both open field and polyhouse condition at a spacing of 2 m x 2 m in CRD with five plants per variety. Compared to the plants grown in open field, the plants grown under polyhouse showed an early flowering (75.68 days), minimum fruit cavity index (15.47 %), zero disease incidence to PRSV, maximum TSS (13. 67 ˚B) and total sugars (11.26 %) in CO-7 while maximum fruit length (18.84 cm) and fruit mass (2605.34g) were observed in CO 7. It was also observed that CO-7 grown under polyhouse showed an increased yield (55.20 kg/plant) as compared to the plants under open condition. Hence, it can be concluded that CO-7 can be successfully cultivated under polyhouse condition in Kerala with good yield and quality.

Evaluating the performance of farmer-managed fruit trees in participatory homestead trials of semi-arid Ethiopia: the case of East Shoa Zone, Oromia

ABSTRACT The growth of farmer-managed tree seedlings (FMTS) is pramount to impact agroforestry technology adoption. This paper presents the performances of FMTS of 3 avocado varieties (Hass, Ettinger and Fuerte) and one papaya variety (Solo) in three districts (Dugda, Adamitulu, and Lome). Thirty-six trials were established (12 trials per district) in 36 homsteads selected purposively. Each homestead was considered as a block in RCBD. Watering frequency was found higher in Dugda than the remaining districts. Seedling survival exhibited significant differences (α<0.05) among varieties with in and across districts. Survival of Solo papaya was significantly higher (α<0.05) at Dugda (91%) than in Adamitulu and Lome. Hass avocado reveled significantlly higher survival (91%) at Dugda than Ettinger and Fuerte across districs. Significant differences in Plot Volume Index (PVI, an integrative growth measurement) among varieties within and across districts indicated that seedling growth was influenced by variety, planting site and, watering fequency. PVI of Hass at Dugda was significantly higher (by 35% and 110%) than that of Ettinger and Fuerte, respectively. PVI of Solo Papaya at Dugda was significantly higher (α<0.05). Hass was found promising avocado variety for planting locations considered, and frequent watering was found crucial for good growth of the tested plant varieties.

Integrated Nutrient Management of Papaya (Carica papaya L.): Application of Microbial Consortium Enriched Organic Manures for Yield and Fruit Quality Enhancement

An experiment was undertaken to study the effect of integrated nutrient management on growth, yield and quality of papaya under Kerala conditions. The trial was conducted in RBD. Papaya variety used for the study was Surya with ten treatments. The results revealed that the applications of microbial consortium enriched organic manures in papaya plants (NPK (25 %) as PGPR mix-1 enriched vermicompost along with N, P and K 75% of recommended dose of fertilizers fertilizers ( 240 N: 240 P2O5: 480 K2O (g plant-1) + 10 kg FYM) resulted in the highest number of fruit production, enhanced fruit weight , total fruit yield per plant. It also resulted in the production of fruits with highest fruit length, girth, pulp percentage and flesh thickness. Fruit quality characters like high TSS, less acidity, higher carotenoid content, ascorbic acid content, highest total sugar content and reducing sugar content were enhanced in papaya. Organoleptic characters of fruit were also enhanced by adopting integrated nutrient management in papaya . Papaya being an important fruit crop of Kerala gaining importance nowadays. Integrated nutrient management (INM) involves efficient and judicious supply of all major and micro components of plant nutrients including local available resources on sustainable basis. Papaya is known to be a heavy feeder of nutrients (NPK) and information regarding the effect of INM is more important

Evaluation of Improved Hermaphrodite Papaya (Carica papaya L.) Varieties for Growth, Yield and Quality at Teppi, Southwestern Ethiopia

The fruit with the most commercial significance in the Caricaceae family is the papaya (Carica papaya L.). The fruits are very valuable in terms of nutrition, economy, and medicine. One of the things restricting its production and productivity in Ethiopia is the lack of improved cultivars. In order to determine the optimum high yielding, pest and disease tolerant, variety/ies for the target area, this study was carried out to evaluate the performance of improved papaya varieties. Three newly released papaya varieties were brought to Teppi agricultural research center from Melkassa agricultural research center for evaluation over the cropping seasons of 2019/20 to 2020/21. The experiment was laid out in randomized complete block design and replicated three times. The mean yield was ranged from 34.24 to 90.08 t/ha for Braz-HS1 and Meki-HL1 varieties respectively. The findings of the study revealed that, based on the recorded parameters Meki-HL1 variety performed better followed by Koka-HM1 variety and these two varieties will be recommended for the surrounding farmers for wider production. Further study should be carried out with improved varieties to improve papaya production and nutritional security as well, especially in southwestern Ethiopia.

EFFECT OF CRYO-STORAGE ON STORABILITY OF PAPAYA SEEDS

A fruit crop of significant economic importance, papayas are often propagated from seeds. Based on their freezing injury even at low moisture content, papaya seeds are classified as "intermediate seeds"; and rapid loss of viability in storage is assumed to be the primary cause of poor germination; thus, the development of appropriate technology is necessary to prolong the storability of papaya seeds. One such effective technology for prolonged seed storability is cryo-storage. The purpose of the current study was to investigate how cryostorage affects papaya seed storability. For this investigation, TNAU Papaya variety CO 8 seeds were utilised. Five distinct treatments were applied to the seeds, which were then kept for approximately six months at liquid nitrogen's vapour phase (-140°C). The biochemical parameters such as protein content, free amino acid, total carbohydrates, free sugars, total lipids, and dehydrogenase activity were measured on a monthly basis along with seed germination and seedling vigour in the cryopreserved papaya seeds. Results indicated that papaya seeds that had been vitrified (seeds with a 10% moisture content that had been soaked in loading solution for 20 minutes, then immersed in Plant Vitrification Solution 2 (PVS2) for another 20 minutes, packed in cryovials and kept in cryo-storage at -140°C) compared to papaya seeds that were packed in cloth bags and stored in ambient conditions had higher seed germination, dry matter production and vigour index at six months after storage. In comparison to fresh seeds, the results also showed that, after six months of cryo-storage, biochemical proximate, protein content, total carbohydrate content, total lipid content, and dehydrogenase activity were maintained in the aforementioned treatment with minimum reduction, while electrical conductivity, free amino acid content, and free sugar content gradually increased with the storage period at minimum rate. In crux, vitrified papaya seeds packaged in cryovials and kept in cryo-storage at -140°C preserved their quality for six months, meeting Indian Minimum Seed Certification Standards. This could be useful for storing papaya seeds for longer periods of time. Keywords: Papaya, vitrification, differential scanning calorimetry, liquid nitrogen, cryo-storage

Breaking the Dormancy of Some Varieties of Papaya (Carica Papaya L.) Soaking KNO3 Solution

Dormancy is a state of stopping growth experienced by plants. This research aimed to determine the concentration of KNO3 solution in breaking dormancy in several papaya varieties. The research method used in this research was a completely randomized design (RAL) Factorial with 2 treatment factors. The first factor is variety (V), consisting of V1 = Callina, V2 = California, and V3 = Local. The second factor is the concentration of the KNO3 (K) solution consisting of: K0 = Control, K1 = 10%, K2 = 20% and K3 = 30%. The variables observed were growth synchrony and germination speed index. Data analysis uses analysis of variance. The variables observed were growth synchrony and germination speed index. An analysis of variance was used to test the effect of treatment. To test mean differences between treatments, honest significant difference tests, regression, and correlation were carried out at a test level of 5%. The research results showed that seed variety had a significant effect on growth synchrony and germination speed index. The concentration of KNO3 solution has a significant effect on growth synchrony and germination speed index. The interaction between the variety and concentration of KNO3 solution had no significant effect on all observed parameters.

In silico identification of papaya genome-encoded microRNAs to target begomovirus genes in papaya leaf curl disease.

Papaya leaf curl disease (PaLCuD) is widespread and classified in the genus begomovirus (Geminiviridae), disseminated by the vector whitefly Bemisia tabaci. RNA interference (RNAi)-based antiviral innate immunity stands as a pivotal defense mechanism and biological process in limiting viral genomes to manage plant diseases. The current study aims to identify and analyze Carica Papaya locus-derived capa-microRNAs with predicted potential for targeting divergent begomovirus species-encoded mRNAs using a 'four integrative in silico algorithms' approach. This research aims to experimentally activate the RNAi catalytic pathway using in silico-predicted endogenous capa-miRNAs and create papaya varieties capable of assessing potential resistance against begomovirus species and monitoring antiviral capabilities. This study identified 48 predicted papaya locus-derived candidates from 23 miRNA families, which were further investigated for targeting begomovirus genes. Premised all the four algorithms combined, capa-miR5021 was the most anticipated miRNA followed by capa-miR482, capa-miR5658, capa-miR530b, capa-miR3441.2, and capa-miR414 'effective' papaya locus-derived candidate capa-miRNA and respected putative binding sites for targets at the consensus nucleotide position. It was predicted to bind and target mostly to AC1 gene of the complementary strand and the AV1 gene of the virion strand of different begomovirus isolates, which were associated with replication-associated protein and encapsidation, respectively, during PaLCuD. These miRNAs were also found targeting betaC1 gene of betasatellite which were associated with retardation in leaf growth and developmental abnormalities with severe symptoms during begomovirus infection. To validate target prediction accuracy, we created an integrated Circos plot for comprehensive visualization of host-virus interaction. In silico-predicted papaya genome-wide miRNA-mediated begomovirus target gene regulatory network corroborated interactions that permit in vivo analysis, which could provide biological material and valuable evidence, leading to the development of begomovirus-resistant papaya plants. The integrative nature of our research positions it at the forefront of efforts to ensure the sustainable cultivation of papaya, particularly in the face of evolving pathogenic threats. As we move forward, the knowledge gained from this study provides a solid foundation for continued exploration and innovation in the field of papaya virology, and to the best of our knowledge, this study represents a groundbreaking endeavor, undertaken for the first time in the context of PaLCuD research.

Identification and Validation of Key Genes Related to Preferred Flavour Profiles in Australian Commercial Papaya (Carica papaya L.).

Commercial papaya varieties grown in Australia vary greatly in taste and aroma. Previous profiling has identified undesirable 'off tastes' in existing varieties, discouraging a portion of the population from consuming papayas. Our focus on enhancing preferred flavours led to an exploration of the genetic mechanisms and biosynthesis pathways that underlie these desired taste profiles. To identify genes associated with consumer-preferred flavours, we conducted whole RNA sequencing and de novo genome assembly on papaya varieties RB1 (known for its sweet flavour and floral aroma) and 1B (less favoured due to its bitter taste and musty aroma) at both ripe and unripe stages. In total, 180,368 transcripts were generated, and 118 transcripts related to flavours were differentially expressed between the two varieties at the ripe stage. Five genes (cpBGH3B, cpPFP, cpSUS, cpGES and cpLIS) were validated through qPCR and significantly differentially expressed. These genes are suggested to play key roles in sucrose metabolism and aromatic compound production pathways, holding promise for future selective breeding strategies. Further exploration will involve assessing their potential across broader germplasm and various growth environments.

Characterization of gynodioecious Pune selection lines of papaya

Among major global threats to papaya cultivation, papaya ringspot virus (PRSV) is the most challenging one. In the absence of any PRSV resistant commercial papaya cultivar, PRSV management is restricted to minimizing yield losses. ICAR-Indian Agricultural Research Institute, Regional Station, Pune has developed PRSV tolerant dioecious papaya lines, Pune Selection (PS)-1, PS-2, PS-3 and PS-5. Being dioecious these lines have limited acceptability among farmers. Gynodioecious population from these lines were developed and characterized. They are numbered PS-1-1, PS-2-1, PS-3-1 and PS-5-1. These lines were characterized against prevailing commercial gynodioecious cultivar, Red Lady, for five generations. The average plant height of PS-2-1 and PS-5-1 (183 cm) was more than Red Lady (158 cm), however, stem girth of all lines was lesser than Red Lady. The fruiting height of all lines was less than Red Lady (87 cm). Length of the fruiting column of all lines was more than Red Lady (37 cm), except in PS-1-1. Fruit yield of all lines was more than Red Lady (16 kg/plant). Intensity of PRSV infection in Red Lady (48%) was considerably more than all lines. These lines can be used for developing PRSV tolerant gynodioecious papaya variety.

MELEIRA IMPACTS IN THE PROFITABILITY OF PAPAYA PRODUCTION IN THE EXTREME SOUTH OF BAHIA

This study evaluated the losses caused by meleira in the profitability of the papaya production in the extreme south of Bahia. The evaluation was based on the technical coefficients of the papaya system of integrated production for the south of Bahia, and information obtained from producers and technical consultants in the municipalities of Eunápolis, Itabela and Porto Seguro. The technical coefficients considered present the quantitative needed for one hectare of papaya, from Formosa and Hawaii groups under irrigated conditions. Based on these coefficients, the profitability analysis was performed. For calculations of the damage caused by meleira, losses of 5%, 10%, 20%, 30%, 40%, 50%, and 60% of eradicated plants were considered. The profitability indicators used were NPV, IRR and BCR. Considering the survey of average prices paid to the producer, the profitability indicators for the Hawaii papaya variety were positive for up to 50% of losses, while those in Formosa were up to 60% of losses.

COMPARING OUTSTANDING PAPAYA LINES FOR SELECTING AND PRESERVING IMPROVED CHARACTERS

Objective: To evaluate outstanding and adapted papaya lines derived from selection to conserve desirable characteristics. Design/methodology/approach: 23 lines of the 'Maradol' type were evaluated at Antunez Michoacan, Mexico. Initially, the plants' height, stem circumference, number of leaves, and first fruit height were recorded. During their development, outstanding plants were identified, and their self-pollinating was promoted. In the fruits, their polar and equatorial circumference, shape index, weight, width and pulp firmness, and soluble solids were assessed. Results: The plants' development was different, their variability between lines allowed identifying morphological characteristics of interest. Only 10 lines had this condition. The number of fruits formed over covered flower buds and collected fruits on formed fruits was reduced. The fruits´ characterization, except for their soluble solids, showed differences. Multivariate analysis indicated variability associated with each principal component. Limitations on study/implications: Currently in Mexico, there are few papaya varieties, the 'Maradol' variety being dominant, and vulnerable to phytosanitary problems over time. However, developing varieties and seed production is challenging and the pollination control of the plants necessarily intervenes. Findings/conclusions: Out of 23 assessed papaya lines, only 43.48 % reported outstanding plants. Inside these lines, between 5 and 10 % of the plants were chosen. In the developmental progress from the covered flower buds’ stage to formed and collected fruits, only 28 % of fruits were obtained. The selected lines showed fruit variability.

Genetic Diversity and Phylogenetic Relationships of Mountain Papaya (Vasconcellea pubescens) in Dieng Plateau Based on Internal Transcribed Spacer Sequence

The Dieng Plateau is an area used to cultivate Mountain Papaya (&lt;em&gt;Vasconcellea pubescens&lt;/em&gt; A.DC.) as a food commodity in Indonesia. Research on diversity and relationships is vital as a first step in Mountain Papaya conservation in the Dieng Plateau. The study aims to determine the genetic diversity and relationship between Mountain Papaya accessions using Internal Transcribed Spacer (ITS) rDNA sequences. Fourteen accessions of Mountain Papaya with different sex distributions and altitudes are amplified using ITS1 and ITS4 primers. The genetic diversity is analyzed using the DnaSP 5.10.1 program. The Maximum Likelihood (ML) approach in MEGA 11 is utilized for assessing phylogenetic tree data based on ITS-rDNA regional sequences. With high haplotype diversity (Hd) values of 1.000 ± 0.027 and high nucleotide diversity (π) values of 0.09674 ± 0.00978, Mountain Papaya exhibits a high level of genetic diversity. Three main clades were identified in the phylogram tree based on the 14 ITS-rDNA sequences of Mountain Papaya. The results of this diversity data can support breeding programs intended to boost Mountain Papaya variety production.

Effect of Postharvest Treatments on Physiological, Chemical, Microbial and Sensory Qualities of Fruits of Papaya Variety Surya

Background: Papaya is a climacteric fruit which is highly perishable due to rapid ripening and softening and susceptibility to biotic or abiotic stresses, resulting in large losses during storage. Traditionally, people resort to the application of synthetic chemical fungicides on to the crop plant and to the fruits during storage, to control the anthracnose caused by the fungus Colletotrichum gloeosporioides on fruits. But their repeated use has caused resistance in microorganisms and toxicity for humans. Hence, nowadays there has been increasing interest in using natural alternatives instead of chemical treatment. Methods: Fruits of papaya variety Surya collected at fully mature green stage were subjected to different postharvest management practices namely precooling, surface sanitization, treatment with chitosan and were packaged in corrugated fibre board boxes and stored till the end of shelf life under ambient condition. Untreated fruits (control) were also used in the study. Result: Fruits coated with chitosan (1%) registered an improved shelf life of 6.33 days as against 4.33 days in control in ambient storage and it further improved the sensory score for all the organoleptic parameters, after six days of storage. 1% chitosan coating also reported significantly lowest physiological loss in weight (3.51 %), ion leakage (60.47%), total soluble solids (12.13 °Brix), total carotenoids (2.34 mg 100g-1), total sugar (7.26%) and reducing sugar (6.34%) and least disease index (16.67%), bacterial count (22.33 cfu/ml × 106) and fungal count (7.33 cfu/ml × 103) after three days of storage when compared with control.

A randomized, open-label, controlled, parallel-group comparative study of two varieties of Carica papaya leaves in dengue-induced thrombocytopenia

Background: Dengue-induced thrombocytopenia can be life-threatening. However, supportive and symptomatic treatment is the current management protocol. The leaf extract of papaya has shown promising results in improving the platelet count. There are many varieties of Carica papaya such as Washington, Honeydew, Coorg honeydew, Solo, Surya, Red lady, C0-1, CO-3, CO-5, C0-6. Aims and Objectives: The aim is to evaluate the efficacy and safety of two varieties (“Red Lady” and “Solo”) of C. papaya fresh leaf extract in patients diagnosed with dengue-induced thrombocytopenia. Materials and Methods: The study was conducted for 2 months, and 18 dengue-confirmed inpatients with thrombocytopenia (platelet count &lt;1,50,000/μL) were enrolled. The study participants were grouped into the standard “Control” group, “Solo” group who received fresh papaya leaf extract “Solo” and “Red Lady” group who received fresh papaya leaf extract of “Red Lady.” About 30 mL of leaf extract was given thrice daily for 5 days. Results: The mean change from the baseline value of platelets was found to be 59,168/µL for the control group and 57,000/µL for Red Lady, and 93,167/µL for the solo group. The study showed a positive trend with improvement in platelet count in “Solo” Variety group when compared to “Red Lady” group and the control group. The safety results showed that both the papaya leaf extracts have a favorable safety profile. Conclusion: There was an improvement in platelet count in “Solo” variety group when compared to “Red Lady” and the control group. However, the results were not statistically significant. No adverse events were observed during the study.

Caracterização morfológica e agronômica de mamão regional (Carica papaya L.)

The aim of this study was to characterize a papaya variety grown in Alto Solimoes, Amazonas, identifying the most important descriptors for recognizing the variety and describing the variety based on its morphological and agronomic characteristics. The study consisted of 30 plants. The qualitative data was described, while the quantitative data was submitted to descriptive statistical analysis. The results showed that the variety has peculiar characteristics, such as purple-medium stem pigmentation, the absence of hermaphrodite flowers and the light orange color of the fruit pulp at the horticultural maturity point. Thus, preserving this variety is essential, considering its local commercial relevance and its potential for future genetic improvement programs.

Efficient genetic transformation of papaya using RNAi CP gene against papaya ringspot virus

Papaya Ring Spot Virus disease is wide spread across papaya growing countries of the world and is one of the major impediments in successful papaya cultivation. Genetically engineered papaya varieties viz., SunUp and Rainbow have already been developed and commercialized in USA using coat protein mediated resistance. However, transgenic papaya conferring resistance to papaya ringspot virus has not been developed in India till date due to lack of suitable genetic transformation protocol for Indian papaya varieties and unavailability of coat protein gene construct for harbouring broad-spectrum resistance. The current study reports an efficient gene delivery mechanism in papaya. Young globular embryos infected for 30 minutes with Agrobacterium tumefaciens strain LBA 4404 harbouring hairpin loop of truncated coat protein gene and subsequently co-cultivated in presence of 100 pM acetosyringone and 1mM spermidine in dark for 72 hours gave rise to independent transgenic events characterized by PCR, dot blot hybridisation and RT-PCR.

Foliar Application of Nutrient Formulation to Enhance Growth and PRSV Tolerance in Commercial Varieties of Papaya

Papaya is an important tropical fruit crop mainly cultivated for fresh fruit as well as papain production. Papaya ring spot virus (PRSV) is a major threat to papaya cultivation which results in severe yield loss. A field experiment was carried out to study the efficacy of a nutrient formulation on the growth attributes and PRSV incidence in commercial papaya varieties at HC&amp;RI, TNAU, Coimbatore during 2022 -2023. The study involved two main plots viz., M1- TNAU Papaya CO 8 (dioecious variety) and M2- Red Lady (gynodioecious variety) and two sub plots viz., S1- Foliar spray of nutrient formulation at monthly interval (3rd, 4th, 5th, 6th and 7th MAP) and S2- Control (without spray) with 13 replications in split plot design. The results revealed that improved growth attributes and lesser incidence of PRSV at flowering stage were registered in treatment with foliar application of nutrient formulation from 3rd to 7th MAP at monthly interval in both TNAU Papaya CO 8 and Red Lady when compared to their respective control and among the varieties TNAU Papaya CO 8 responded comparatively better than Red Lady.

Physiological characterization of Carica papaya L. variety UN Cotové

The application of plant physiological principles in genetic improvement allows to approach with greater ease, the solution of the limitations of the agricultural production in an integral and articulated way. For this reason, physiological indicators were used as criteria for the selection of UN Cotové papaya variety plants, which serve as parents for a species breeding program. Eighteen individuals were selected for monthly gas exchange measurements, chlorophyll fluorescence, and leaf area index. The data analysis was done with multivariate non-hierarchical clustering and factorial analysis. Two clusters were generated that did not group morphotypes regarding the gas exchange, quantum yield of Photosystem II (PSII), and leaf area index (LAI). ANOVA established significant differences between plants for most variables. The means were compared with the Tukey test (P &lt; 0.05). Six plants presented better yield, positioning them as a promising material to obtain a papaya variety with outstanding characteristics under tropical dry forest (TDF) conditions.

Study the Indicators of germination and growth of two varieties of papaya

The study was conducted in the Seyhan Agricultural Reserve in the city of Siba in Basra Governorate during the period (2019-2021), for the purpose of knowing the extent of the success of the cultivation of Carica papaya L. and identifying the indicators of germination and growth of two varieties of local and Red lady papaya. Papaya seeds were grown under standard germination conditions.&#x0D; The results of the study showed the superiority of the Red Lady cultivar in the percentage of germination, seed germination speed and stem diameter, while the local cultivar was superior in seedling height.

Study the Indicators of germination and growth of two varieties of papaya

The study was conducted in the Seyhan Agricultural Reserve in the city of Siba in Basra Governorate during the period (2019-2021), for the purpose of knowing the extent of the success of the cultivation of Carica papaya L. and identifying the indicators of germination and growth of two varieties of local and Red lady papaya. Papaya seeds were grown under standard germination conditions.The results of the study showed the superiority of the Red Lady cultivar in the percentage of germination, seed germination speed and stem diameter, while the local cultivar was superior in seedling height.