Coriander Varieties Research Articles

Determination of mutagenic effectiveness and efficiency of gamma rays and EMS in Coriander

Coriander is a dual purpose vegetable used as leaf as well as a spice, along with medicinal properties. Owing to its compound umbel and difficulty in crossing programme, mutation breeding is the available source to create variability. Mutagenesis is a well-recognized tool to expand genetic variability. The present investigation involves mutagenesis in coriander cv. Sindhu with physical (gamma rays) and chemical mutagens (EMS) and their combinations. The mutation frequency, mutagenic effectiveness and mutagenic efficiency were calculated for the gamma rays, EMS and their combination doses. In gamma rays, a drop in mutagenic effectiveness was observed at 10Kr followed by increase with higher concentration. Mutagenic effectiveness due to gamma rays, ranged from 6.5 to 8.51 due to EMS ranged from 42.09 to 49.55 and due to combination treatments ranged from 12.17 to 18.80 (5kr+0.2% EMS) in coriander variety, Sindhu. The mutagenic efficiency based on survival ranged from 1.78 to 4.0, injury ranged from 3.83 to 8.2, sterility ranged from 2.37 to 5.18 indicating differential response to different mutagens. The combination of gamma rays and EMS at lower and moderate doses expressed its worth to create higher mutation frequency by manifesting higher efficiency than the individual mutagens single-handed.

Mathematical approach used for the assessment of foliar application of biostimulant and fertilizers to coriander varieties (Coriandrum sativum L.)

The biostimulants and fertilizers applied as foliar spray or directly to soil play a crucial role in enhancing the development and quality of coriander by supplying essential macro- and micro-nutrients. The ecological approach to reducing soil fertilizers prioritizes the utilization of foliar nutrients. The objective of this study was to investigate the impact of foliar applying products on the seed yield, essential oil content and composition of coriander cultivars in Central Bulgaria. The field trial was carried out on alluvial-meadow soil type in the region of Plovdiv, Central Bulgaria, in the period 2020–2022. The experiment was conducted using the approach of fractional parcels with four repetitions. Three foliar products were examined in the relevant rates: Energy 20–8–60 – 25 L ha−1; Fulvin 40–22 – 80 L ha−1; Isabion – 30 L ha−1. The treated plants were compared to an untreated control. The tested products were applied in the end of the budding stage to five coriander varieties: Yantar, Moroccan, Mesten Drebnoploden, Thüringen and Marino. Results showed that the application of foliar products, compared to the control, led to an increase in seed yield by 4.2–9.6 % and essential oil content up to 11.8 %. Statistical analysis has demonstrated that the utilization of the Energy 20–8–60 has the most significant impact on the concentration of essential oils, while Isabion has the biggest influence on the quantity of seeds produced. The Isabion biostimulant increased the content of the main compound – β-linalool in the essential oil of Moroccan variety up to 8.9 % and Marino and Mesten Drebnoploden by 3.6 and 5.4 % respectively, compared to the control. The foliar application of Energy 20–8–60 fertilizer led to a decrease of β-linalool percentage in Marino, Moroccan, Mesten Drebnoploden and Thüringen. All studied varieties exhibited the presence of aldehyde 2E-tridecen-1-al under the impact of foliar fertilizers, whereas the untreated plants did not.

Response of coriander varieties to various levels of fertility under different cutting

Response of coriander varieties to various levels of fertility under different cutting

Fusarium Wilt of Coriander: Root Cause Analysis and Varietal Tolerance Development.

Since 2012, growers of coriander, Coriandrum sativum L., in Israel have been suffering from summer wilting that can result in entire fields collapsing. The current study aimed to determine the cause of the phenomenon and find a genetic solution to the problem. The disease was reproduced in a growth chamber using naturally-infested soil from a commercial field. Wilt became apparent within two weeks, and after ten weeks, all plants died compared to plants in sterilized soil from the same source. Fusarium oxysporum was isolated from infected plants, and Koch's postulates were completed. Sequence analysis of the Elongation Factor (EF1α) encoding gene of the pathogen had a 99.54% match to F. oxysporum f. sp. coriandrii. Several coriander varieties were screened for resistance or tolerance to the disease. In four independent experiments, only the cultivar 'Smadi' showed high tolerance, while other genotypes were susceptible. In a trial in a naturally infested field, the cultivar 'Smadi' outperformed the commercial cultivar 'Blair'. 'Smadi' provides a cropping solution to many Israeli farmers, yet this winter cultivar bolts early in the summer. There is a further need to characterize the tolerance mechanism and inheritance for informed breeding of late-bolting Fusarium-resistant coriander.

Seed yield limitations of coriander (Coriandrum sativum L.) based on plant structure analysis

The present study describes the dependencies of key productivity and growth indicators of coriander plants on sowing rate and fertilization parameters. The aim of the research was to establish the patterns of coriander yield formation through the prism of plant structure considering modifications in nutrient conditions and sowing rates. Graphical models and correlation matrices were developed for three coriander varieties with similar morphological features (Oksanit, Nectar, Caribe). Data from field studies conducted in 2013–2015 in the Forest-Steppe zone of Ukraine serve as the basis for model construction. Two variable technological elements were studied (sowing rate of seeds; fertilization system) to determine the dependencies of biometric indicators for each variety. 3D graphical models of dependency for structural elements showed advantages over linear models. Seed yield of coriander in the study ranged from 1.24 t/ha with the lowest sowing rate to 1.91 t/ha with a sowing rate of 2.5 million seeds/ha. Maximum essential oil yield for cv. Oksanit was 46.4 kg/ha with a seed yield of 2.16 tons/ha. Cv. Nectar forms 45.6 kg/ha of essential oil with a seed yield of 2.01 tons/ha, and cv. Caribe forms 40.6 kg/ha with a seed yield of 2.06 tons/ha. Absence of statistically significant correlation between seed yield and essential oil output points to the peculiarities of the experiment. Research factors have opposing effects on the formation of essential oil, and consequently, its yield from the seed yield. The value of the results lies in the potential application of this approach in practice and the implementation of findings in the humid zone. The development of productivity models (like 3D plots) across a range of multiple factors simultaneously, based on crop structure indicators, allows for the prediction of target crop parameters, enabling the efficient utilization of available resources.

The influence of sowing time on the yield of dill fruits and coriander in the dry steppe zone of Transbaikalia

This article describes the history of the industrial development of the essential oil industry and the prospects for cultivating such essential oil crops as dill and coriander, including in the territory of Transbaikalia. Data are provided on the volume of essential oil production in Russia in different years. The noted morphobiological features of dill and coriander suggest the possibility of obtaining a high yield of fruits when they are cultivated by agricultural producers in the dry steppe zone of Transbaikalia. For the first time, in the conditions of the dry steppe zone of Transbaikalia, field research was carried out to study the effectiveness of some agricultural practices for cultivating zoned varieties of dill and coriander. The results of three years of research (2021-2023) on the formation of fruit yield of plants of the Umbrella family with a one-year development cycle - dill (Gribovsky, Lesnogorodsky varieties) and coriander (Karibe variety) in the driest soil-climatic zone of Transbaikalia are summarized. It was revealed that the heat supply of the growing season affects the formation of the fruit yield of the studied crops. The highest fruit yield was obtained from the Gribovsky dill variety at an average sowing time, and from the Lesnogorodsky variety when sowing early. At the same time, the Gribovsky variety is superior in yield to the Lesnogorodsky variety. The coriander variety Caribe provided the maximum fruit yield at a late sowing time in all years of research. In general, the soil and climatic conditions of the dry steppe zone of Transbaikalia make it possible to obtain a harvest of aromatic dill and coriander at the level of other regions of Russia, which determines the future development of essential oil crop production in our region.

Pratap Raj Dhania-1 (RKD 18): Promising coriander (Coriandrum sativum L.) variety for farmers of South Eastern Rajasthan

Pratap Raj Dhania-1 (RKD 18): Promising coriander (Coriandrum sativum L.) variety for farmers of South Eastern Rajasthan

Estimation of efficiency of metribuzin to weeds control in coriander crops

There are no chemicals in Russia to protect vegetative coriander from a complex of dicotyledonous and monocotyledonous weeds. The expediency of using metribuzin for this purpose was assessed. Field small-plot trials with herbicide Zenkor Ultra SC (metribuzin, 600 g/l) were carried out in three agricultural territories with differing climatic conditions: Krasnodar, Omsk and Astrakhan Regions. Weed counts were using the quantitative and weight method. The efficiency of herbicide was calculated to the control without treatment. As a result of trials, three groups of weeds were identified: 1) species highly sensitive to herbicide: Chenopodium album L., Amaranthus retroflexus L., Salsola tragus L. (efficiency 90-95%); 2) species medium sensitivity: Solanum nigrum L., Polygonum aviculare L. (efficiency 50-65%); 3) poorly controlled weeds: Ambrosia artemisiifolia L., Galium aparine L. (efficiency less 30-40%). Increases yield of seeds of coriander variety Yantar after Zenkor Ultra SC use amounted to 26.1-37.3%. Phytotoxicity was noted on varieties Ranniy and Santo in one of the years and this needs future estimation sensitivity of varieties to metribuzin.

Lytic and Molecular Evidence of the Widespread Coriander Leaf Spot Disease Caused by Alternaria dauci.

Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic properties. While coriander leaf blight disease was previously confined to India and the USA, this study presents new evidence of its outbreak in Africa and the Middle East caused by Alternaria dauci. Infected leaves display irregular chlorotic to dark brown necrotic lesions along their edges, resulting in leaf discoloration, collapse, and eventual death. The disease also impacts inflorescences and seeds, significantly reducing seed quality. Koch's postulates confirmed the pathogenicity of the fungus through the re-isolation of A. dauci from artificially infected leaves, and its morphology aligns with typical A. dauci features. Notably, this study identified strong lytic activity (cellulase: 23.76 U, xylanase: 12.83 U, pectinase: 51.84 U, amylase: 9.12 U, and proteinase: 5.73 U), suggesting a correlation with pathogenicity. Molecular characterization using ITS (ON171224) and the specific Alt-a-1 gene (OR236142) supports the fungal morphology. This research provides the first comprehensive documentation of the pathological, lytic, and molecular evidence of A. dauci leaf blight disease on coriander. Future investigations should prioritize the development of resistant coriander varieties and sustainable disease management strategies, including the use of advanced molecular techniques for swift and accurate disease diagnosis to protect coriander from the devastating impact of A. dauci.

Еvaluation of productivity and adaptability of coriander varieties under forest-steppe conditions of the middle Volga region

Coriander is a common essential oil crop and a source of a number of valuable products. Coriander is grown mainly for oil seeds (fruits) and spicy greens (cilantro). The article presents a comparative assessment of the productivity and adaptability of 14 varieties of coriander in the conditions of the Penza region (forest-steppe of the Middle Volga region). Over the years of research (2020-2022), the duration of the growing season of coriander was, on average for varieties, 89-109 days. Early ripening was noted in varieties Karibe, Borodinsky and Silach (89-92 days). The yield of coriander seeds ranged from 1.41 to 1.83 t/ha, with the maximum values in the Nectar and Santo varieties (1.75 and 1.83 t/ha), 0.15-0.42 t/ha higher other varieties (LSD05 = 0.11). The highest content of fatty oil was noted in the varieties Commander (20.09 %), Venera (19.58 %) and Santo (19.04 %). All varieties had a fairly high adaptive potential: the coefficient of adaptability ranged from 0.85 to 1.19, with the highest values in varieties Nectar (1.19), Santo (1.13) and Karibe (1.12). These varieties had the highest breeding value (1.32-1.42). According to the values of the indicator of the level of stability of the variety (19.09) and the stability index (13.66), the variety Karibe was characterized by the highest stability of the trait "yield". The analysis of the crop structure showed that the varieties Nectar (4.89 g) and Santo (5.49 g) were selected according to the productivity of one plant. The largest fruits were formed in varieties Taiga (10.26 g), Santo (10.94 g) and Karibe (10.85 g). According to the number of fruits in one umbel, 7 varieties are of the greatest interest, in which this indicator reached more than 40 pieces. The maximum number of umbel 21.6 and 22.6 was formed by varieties Nectar and Santo. Evaluation of coriander varieties showed their high adaptive capabilities in contrasting weather conditions of the Penza region.

Influence of seed priming and foliar application of micronutrients on Phenophasic development and quality attributes of coriander (Coriandrum sativum L.) variety – Jawahar Dhaniya 1

Influence of seed priming and foliar application of micronutrients on Phenophasic development and quality attributes of coriander (Coriandrum sativum L.) variety – Jawahar Dhaniya 1

Investigation on Effect of Sowing Dates and Cutting Management on Physiological Characteristics of Coriander (Coriandrum sativum L.) variety JD – 1

Investigation on Effect of Sowing Dates and Cutting Management on Physiological Characteristics of Coriander (Coriandrum sativum L.) variety JD – 1

Growth and yield of AGCr-1 coriander affected by plant geometry and nutrition

A study was conducted during Rabi season of 2015-16 and 2016-17 at the Research Farm of ICAR-NRC on Seed Spices, Ajmer, Rajasthan to assess the influence of plant geometry and different levels of nutrition on vegetative growth and yield of coriander variety Ajmer Green Coriander-1 (AGCr-1). The treatments comprised of three crop geometry (25 cm x 5 cm, 25 cm x 7.5 cm and 25 cm x 10 cm) and three levels of NPK (40:25:20 kg ha-1, 50:35:25 kg ha-1 and 60:45:30 kg ha- 1) laid out in Randomized Factorial Block Design. The vegetative growth attributes viz., plant height at different growth stages, length of first leaf, length of second leaf, number of basal leaves plant-1, primary branches plant-1 and secondary branches plant-1 recorded the highest level with the application of 60:45:30 kg ha-1 NPK combined with (25 cm x 7.5 cm) crop geometry. Similarly, yield parameters like number of umbel plant-1, number of umbellates umbel-1 and seed yield (1253.0 kg ha-1) were also maximum with the same treatment.

Efficiency of joint glycine and auxin analogs foliar treatment on coriander seeds yield and essential oil quality

Coriander is the leading essential oil crop and occupies vast areas, both in our country and abroad, and is be used to produce linalool, with subsequent processing into other aromatic substances. Increasing its yield and quality of raw materials with the help of modern preparations is an urgent problem of essential oil production.Purpose of the work: increasing the productivity of coriander with combined use of foliar treatments with glycine and auxin-containing preparations for directed control production process in coriander seed.Material and methods. As objects for studying the effect of the drug, varieties of coriander sowing Yantar and Avangard were chosen. Plants were sown in the first or third decade of April, depending on the conditions of the year, using a SZT-3.6 seeder with row spacing of 15 cm. The seeding rate was 25 kg/ha, the seeding depth was 2 cm. concentration 10 mg/l. Drug concentrations: IAA-glycol phosphate – 25, 50 and 100 mg/l, DvaU - 2 ml/l. Treatment with a solution of IUKGF and DvaU was carried out in the phase of budding-beginning of flowering. The cutting was carried out during the period of browning of seeds on the central umbrella. The content of essential oil was determined according to SP XIV method 1.Results. As a result of the research, a positive effect of foliar treatments with glycine was revealed, both on the yield and on the content of essential oil in the raw material of coriander seed varieties Yantar and Avangard. Based on the results obtained, the effective concentration of the drug is determined not only by the characteristics of the variety, but also by weather conditions, when, depending on the conditions during the processing period and the previous harvest, different aspects of the drug action appear. The maximum increase in the collection of essential oil of coriander variety Yantar (11.1 kg/ha) was noted during the combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and IAA-GF at a rate of 50 mg/l in the budding phase; varieties Avangard (8.6 kg/ha) - with combined treatment of plants with glycine at a rate of 10 mg/l in the leaf rosette phase and DvaU at a rate of 2 ml/l in the budding phase.

TECHNOLOGICAL METHODS FOR CORIANDER CULTIVATION ON VERTICAL RACKS

The research is aimed at specifying the possibility of cultivation and gain of marketable coriander products with minimum energy consumption in photoculture conditions. Growth of vegetative part, root system, the quality and photosynthetic activity of coriander (Coriandrum sativum L.) were evaluated with application of LED lighting in different spectral ratios. Coriander varieties were grown hydroponically on vertical racks using flooding technology. Illumination lamps based on blue, green and red LEDs were used as lighting, with a total PAR intensity of 150 mmol/m²s. Far red 6-12% was added to the main spectrum of illumination. The results showed that application of LED light (mainly red and green, and to a lesser extent blue light) significantly influenced a wide range of parameters of plant growth and development, including photosynthetic activity of pigments, the biomass of shoots and roots. The photosynthetic ability of the leaves was significantly higher under the radiation in the second variant with the addition of far red spectrum of 6% compared to other illumination variants. The root system parameters in different variants of the experiment differed slightly. The most appropriate ratio of LED emission spectra are B(23) :G(53):R(74) mmol/m²s with a far red spectrum share of 12% (option 1) for 'Santo' variety and B(21):G(59) :R(70) mmol/m²s with 6% far red (option 2) for 'Caribe' variety. The conditions of light culture ensure formation of economically useful biomass of coriander plants in a short period of 22-25 days, on average, in the amount of 1.81 kg/m². Studies showed that in case of growing coriander varieties on vertical hydroponics racks, it is possible to obtain high-quality green marketable products in the early stages at threshold values of photosynthetic irradiance.

Evaluation of Yield Contributing Traits in Coriander (Coriandrum sativum L.) Varieties under Drought Conditions

Background: Coriander (Coriandrum sativum L.) also called ‘Dhania’ (Hindi), belongs to Apiaceae family is an important seed spice, tall herbaceous annual diploid (2n=22) crop grown around the world for its fruits as well as for tender green leaves. Methods: The present investigation was done to assess the genetic variations among coriander genotypes for drought stress. Eight Coriander varieties viz. Rcr-20, Rcr-41, Rcr-435, Rcr-436, Rcr-446, Rcr-475, Rcr-684 and Rcr-728 were evaluated in randomized block design with three replications at the research farm of S.K.N. College of Agriculture, Jobner (Rajasthan) during Rabi, 2016. The plot was irrigated at regular interval in control condition while in drought condition plots were maintained under rainfed condition. The various morpho-physiological or yield and yield contributing parameters were recorded at maturity stages and after harvesting. The mean performance of the genotypes was decreased under drought stress in comparison to control condition for all parameters. Result: The coriander varieties Rcr-446 and Rcr-20 were found to be drought tolerant varieties with less effect of water stress on yield contributing traits. While, coriander varieties Rcr-435 and Rcr-728 maintained higher seed yield along with number of umbels per plant, seeds per umbel, test weight, seed yield, biological yield, harvest index under control conditions. Thus, these varieties may be used as drought tolerant varieties for further investigation.

Impact of Different Fertilizer Sources on Nutrient Uptake in Coriander for Kymore Platueau and Satpura Hills of Madhya Pradesh

Coriander is an important spice crop grown through out the world. As organic manures tend to reduce the cost of cultivation and supplement the crop with essential nutrients for growth and development of the plant hence the present study the effect of different fertilizers and their doses on nutrient uptake in coriander variety JD-1 was conducted at Horticulture complex, Department of Horticulture, JNKVV, Jabalpur (M.P.) in the year 2011-2012. The experiment consisted of 12 treatments. Viz fertility levels 100% RDF (Recommended dose of fertilizers) (N50P30K60 kg ha-ˡ) and 50% RDF (N25P15K30 kg ha-ˡ) in combinations with organic manures Viz FYM (Farm yard manure) 20 tonnes/ha-ˡ and 10 tonnes/ha-ˡ, Poultry manure 5 tonnes/ha-ˡ and 2.5 tonnes/ha-ˡ, Vermicompost 5 tonnes/ha-ˡ and 2.5 tonnes/ha-ˡ. Among the organic manures, the maximum nitrogen uptake (47.47 kg ha-1) was observed in poultry manure 5 t ha -1,while the minimum nitrogen uptake (34.08 kg ha-1) in FYM 10 t ha -1.The maximum phosphorus uptake (31.63 kg ha-1) was found in poultry manure 5 t ha -1, while it was minimum (26.20 kg ha-1) in vermicompost 2.5 t ha -1. Potassium uptake was maximum (16.11kg ha-1) in vermicompost 5 t ha -1, while it was minimum (15. 19 kg ha-1) in FYM 10 t ha -1. With regard to fertilizer levels maximum nitrogen (43.91 kg ha-1), phosphorus (30.92 kg ha-1), and potash (15.92 kg ha-1 ) uptake was found with 100% RDF. While it was minimum (37.16 kg ha-1, 26.10 kg ha-1 and 15.13 kg ha-1 respectively) with 50% RDF. In case of interaction effect, the maximum nitrogen (51.96 kg ha-1 ) and phosphorous (34.90 kg ha-1) uptake was in treatment T5 (Poultry manure 5 t ha -1 + 100% RDF) while maximum potassium uptake (16.48 kg ha-1) was found in T9 (Vermicompost 5 t ha -1+ 100% RDF). However, the lowest (14.76 kg ha-1) potassium uptake was found with treatment T4 (FYM 10 t ha -1 + 50% RDF). Hence it is concluded that maxium nutrient uptake is noticed in treatment consisting of Poultry manure 5 t ha -1 along with 100% RDF as compared to other treatments in coriander JD-1.

АНАЛИЗ КОЛЛЕКЦИИ КОРИАНДРА ПОСЕВНОГО ПО СЕЛЕКЦИОННО ЦЕННЫМ ПОКАЗАТЕЛЯМ

Coriander is a valuable, highly profitable essential oil crop. Therefore, the development of new highly productive varieties is the main direction of breeding. The aim of the research was to study the collection of Coriandrum sativum L. (owner – Research Institute of Agriculture of Crimea) by a complex of valuable traits to identify promising samples for breeding purposes. In 2017–2019, under the conditions of the Crimean Foothills (village of Krymskaya Roza, Belogorsky district), we analyzed 164 samples from 30 regions of the world. The territory of the experimental plots belongs to the upper foothill, warm, insufficiently humid agro-climatic region. To compare the parameters, we included five coriander varieties in our study. They were created in the Research Institute of Agriculture of Crimea (‘Yantar’, ‘Ranniy’, ‘Nektar’, ‘Medun’ and ‘Silach’) and registered in the “State Register of Breeding Achievements Permitted for Use” of the Russian Federation. The work was guided by the methodological recommendations on essential oil crops breeding. Plot area – 0.6 m2, double replication. The analysis revealed high variability of the collection samples in terms of the main indicators of productivity: fruit yield (Cv = 37.2 %), content of essential oil (Cv = 51.3 %), yield of essential oil per unit area (Cv = 60.0 %). Optimal weather conditions for the accumulation of essential oil with a high content of linalool (the main component) are relatively low air humidity and increased temperature regime during flowering and fruit ripening. The following samples were identified as sources of valuable traits: vr. 341, vr. 757, vr. 756, vr. 387, VIR 415 with a mass fraction of essential oil in fruits at the level of 2.81–3.62 %; VIR 258, vr. 233, VIR 144, VIR 165, VIR 246, vr. 705 with a fruit yield that reached 52.0–73.9 g per plot. Five samples – VIR 348, VIR 421, VIR 180, VIR 431 and vr. 521 – provided a high amount of essential oil (0.90–1.23 g per plot) because of the combination of the increased yield and essential oil mass fraction.

Discrimination of a selected set of turmeric, ginger, fenugreek and coriander varieties using ISSR markers

DNA fingerprints are unique to individuals and can be used to identify individuals as in the case of conventional fingerprints. Plant DNA fingerprinting make use of various molecular markers for identifying newly released crop varieties and are all the more important in plant variety registration under the PPV&FR Act of 2001. The trade-related intellectual property rights (TRIPS) and the convention on biological diversity (CBD) insist on the establishment of identity and ownership of genotypes for enforcement of their provisions for securing protection to plant varieties as well as for regulating access to germplasm resources. DNA fingerprints, along with morphological markers, can be efficiently utilized for plant varietal identification, detection of duplicates and adulterants. Here in this particular study, the spice samples received at the DNA fingerprinting facility (DNAFF) of ICAR-Indian Institute of Spices Research (ICAR-IISR) from various centres of All India Coordinated Project on Spices (AICRPS) were DNA fingerprinted using inter simple sequence repeat (ISSR) markers. The DNA profile of a candidate variety vis-a-vis check variety is an essential prerequisite during submission of proposal for release of crop variety to central sub-committee on crop standards notification and release of varieties. The new varieties of turmeric, ginger, coriander and fenugreek were compared with the closely resembling check varieties for establishing distinctness for varietal registration. A total of 118 ISSR primers were screened in the above-given crops, to identify the distinct markers identifying the candidate from the check varieties. Using this technique, the DNAFF at ICAR-IISR could facilitate registration of turmeric varieties, Roma, Rasmi and Suroma; ginger varieties Suruchi, Suravi and Suprabha; coriander varieties, Suguna, Susthira and Suruchi, while varieties of turmeric, Uttara Rupanjana and Uttara Ranjini; fenugreek variety Ajmer fenugreek (AFg-5); coriander varieties Ajmer coriander (ACr-2) and Chhattisgarh Shri Chandra Hasini dhaniya-2 (ICS-4) are in the process of getting registration. ISSR markers were found to be appropriate for establishing distinctness of the new varieties of spices for securing varietal registration.

Assessment of Mutagenicity Induced by Different Mutagens in Coriander (Coriandrum sativum L.)

Coriander is one of the most widely used herb. Coriander generally grown for leaf and grain purpose throughout the world. Induction of mutation is considered to be effective in improving various characteristics in plants like seed germination, plant vigour, yield etc. In present study we used three different chemical mutagens Colchicine, Sodium azide (SA) and Methyl methane sulphonate (MMS) on four different varieties of coriander (Green wonder, Cross-91, Mrudul and Surabhi). The study was performed by exposing the seeds of four varieties of coriander (Coriandrum sativum L.) to Colchicine (0.01%,0.02%,0.03% and 0.04%), Sodium azide (SA) at (0.1%,0.2%,0.3% and 0.4%) and Methyl methane sulphonate (MMS) at (0.01%,0.02%,0.03% and 0.04%) respectively. The observations were made on seed germination in field and laboratory conditions and the observations on seedling height, number of primary branches and leaves were made in field conditions. All the mutagens significantly affect the germination and seedling growth. The study revealed that germination percentage, plant height, no. of primary branches and leaves decreased with increase in dose/concentration of mutagen. Among three different chemical mutagen we observed that colchicine is better mutagen for induction of mutagen after that sodium azide (SA) had good results for seed treatment. Some of the concentrations of methyl methane sulphonate (MMS) (0.01% & 0.02%) were better. Among all four varieties of coriander Cross-91 gave better result after that Mrudul and Surabhi respond well to some concentrations. We categories the effectiveness of mutagen to induce mutation in coriander as MMS>SA>Colchicine.