AbstractChanges in the Atlantic Meridional Overturning Circulation (AMOC) and associated Meridional Heat Transport (MHT) can affect climate and weather patterns, regional sea levels, and ecosystems. Direct observations of the AMOC are still limited, particularly in the South Atlantic. This study establishes a cost‐effective trans‐basin section to estimate for the first time the AMOC and MHT at 22.5°S, using only sustained ocean observations. For this, an optimal mapping method that minimizes the difference between surface in situ dynamic height and satellite altimetry was developed to retrieve monthly temperature and salinity profiles from Argo and XBT data along the 22.5°S section. The mean states, as well as the interannual and seasonal changes of the obtained AMOC and MHT were compared with other products. The mean AMOC and MHT for 22.5°S are 16.3 ± 3.2 Sv and 0.7 ± 0.2 PW, respectively, showing stronger transports during austral fall/winter and weaker in spring. The high‐density XBT data available at the western boundary were vital for capturing the highly variable Brazil Current (BC), whose mean and variability was improved compared to other products. At 22.5°S, the North Atlantic Deep Water is divided into two cores that flow along both the western and the eastern boundaries near 2,500 m depth. Our results (a) suggest a greater influence of the western boundary current system on the AMOC variability at 22.5°S, (b) highlight the importance of high‐density in situ data for AMOC estimates, and (c) contribute to a better understanding of the AMOC and MHT variability in the South Atlantic.