Vancouver Harbour Research Articles

Polycyclic aromatic compound and trace metal element residues in Mytilus mussels at marine wildlife hotspots on the Pacific coast of Canada

The Pacific coast of Canada has a rich marine fauna and a growing human population with increasing potential for pollution releases, but there is currently little overlap between marine wildlife hotspots and ongoing biomonitoring efforts for less bioaccumulative contaminants such as polycyclic aromatic compounds (PAC) and trace metals (metals). We surveyed PACs and metals at marine bird breeding colonies in coastal British Columbia in 2018 by analyzing chemical residues in the soft tissue of bivalve Mytilus sp. mussels collected from stations (n = 3) at seven sites. The concentration of sum PACs (∑43PAC) and high molecular weight (HMW) PACs were highest at the Second Narrows colony in Vancouver Harbour, a highly urbanized and industrialized port within the Salish Sea. For conservation areas, two Salish Sea and three Pacific Ocean coast colonies, PACs were generally lower. However, ∑43PAC, ∑HMWPAC, and several HMW congeners at the remote site of Triangle Island, a Marine National Wildlife Area, were not significantly different from Second Narrows. The dominant PAC sources at all sites are likely pyrogenic rather than petrogenic, as suggested by PAC profiles, proportion of parent PACs, and source-indicator congeners. For metals, site differences were found for seven out of eight priority metals, but principal component analysis indicated that site differences, such as high mercury and cadmium at offshore sites, are likely related to environmental and biological variables including salinity, condition index, water temperature, and shell length. Our survey across a broad coastal region shows that PAC and metal biomonitoring programs with mussels should include wildlife hotspots where the exposure of protected vertebrate species to pollutants with low bioaccumulation potential would be less obvious, and shows that collection of data on key covariates (e.g. lipid content, salinity) will be critical to tracking long-term trends and detecting pollution release events.

Geographic Response Strategies on Canada's West Coast

Abstract In 2012, Western Canada Marine Response Corporation (WCMRC) began developing site specific shoreline protection strategies, known at Geographic Response Strategies (GRS) for the entire coast of British Columbia (B.C.). The project started in Vancouver Harbour and has since spread along the Salish Sea and Strait of Juan de Fuca, as well as into Prince Rupert and Kitimat on the northern B.C. coast. Recognizing that B.C. has approximately 27,000 km of coastline (~16,777 miles) and with 450 strategies already developed only within a few hundred kilometres, WCMRC saw a need to automate the GRS development process from data collection all the way to the final GRS output. In conjunction with a local environmental consulting company, WCMRC developed a new sensitivity model. This new model can help the Response Readiness Team quickly assess intertidal sensitivity to oiling based on shoreline type, oil residency index, biological, archaeological, and/or socio-economic features of the shoreline, as well as operational protection feasibility. Now, using ESRI GIS web tools, a GRS can be developed automatically as a geo-referenced PDF, easily exportable to mobile devices for operational use. Overall, the automated enhancements have provided WCMRC with the tools necessary to manage the GRS program for B.C.'s entire coast. This means that more coastline can be assessed far more quickly and GRS's can be developed using fewer human resources. Additionally, if a spill occurs in a more remote area that has not yet had GRS's developed, they can be created within minutes based on the information from the Environment Unit in the Incident Command Post, or initial assessments by responders.

A modeling study on tides in the Port of Vancouver

In the present paper, tides in the port of Vancouver Harbour have been investigated with a high-resolution three-dimensional hydrodynamic model based on FVCOM (Finite Volume Community Ocean Model). The model was evaluated against field observations including tidal elevations and tidal currents, and the evaluation showed that the model was in good agreement with the observational data. Using the model, we first investigated the horizontal distributions of tides, tidal currents, and tidally induced residual circulation, and then investigated the tidal asymmetry and dynamic mechanisms of tidal flows in the harbour. The tidal residual circulation shows a strong spatial pattern, which is associated with the local coastlines and variation of topography. The tidal asymmetry in the harbour is caused by different mechanisms, not only including the traditional factors, such as residual flows, the interaction between M2 and its overtide M4, but also the interaction of principal astronomical tides of O1, K1, and M2. The momentum balance is dominated by terms of the advection and the pressure gradient in First Narrows and Second Narrows, whereas terms of the local acceleration and the Coriolis are also important in the central harbour. The spatial variations of the momentum terms are strongly associated with the local changes in coastline and topography.

A Modeling Study on the Oil Spill of M/V Marathassa in Vancouver Harbour

The M/V Marathassa oil spill occurred on 8 April 2015 in the English Bay. In the present study, the trajectory and the transport mechanism of the spilled oil have been studied by using the three-dimensional and particle-based Oil Spill Contingency and Response (OSCAR) model forced by the Finite-Volume Community Ocean Model (FVCOM). FVCOM provided the hydrodynamic variables used by the oil spill model of OSCAR. The results showed that the fraction of the oil on the water surface and on the shoreline, as well as the amount of oil recovered were affected by the time of the initial release, the overall duration of the discharge, wind and recovery actions. The hindcast study of the M/V Marathassa oil spill showed that the likely starting time for the discharge was between 14:00 and 15:00, on 8 April 2015. The release may have lasted for a relatively long time (assumed to be 22 h in this study). The results of modeling in this study were found reasonably acceptable allowing for further application in risk assessment studies in the English Bay and Vancouver Harbour. The trajectory of the spill was mainly controlled by the tidal currents, which were strongly sensitive to the local coastline and topography of First Narrows and that in the central harbour. The model results also suggested that a high-resolution model, which was able to resolve abrupt changes in the coastlines and topography, was necessary to simulate the oil spill in the harbour.

Observation of A Super Pod of Pacific Harbor Porpoises (Phocoena phocoena vomerina) In the Salish Sea

Abstract The Harbor Porpoise normally occurs in groups of 2 to 5 individuals. We report a sighting of 74 Harbor Porpoises near the entrance to Vancouver Harbor, Canada. When corrected for detection underestimate, this group was possibly as numerous as 1894 individuals, which we call a super pod

Identification of Spilled Oil from the MV Marathassa (Vancouver, Canada 2015) Using Alkyl PAH Isomer Ratios.

On the morning of April 9, 2015, citizens in Vancouver (British Columbia, Canada) awoke to the sight and smell of oil on the shores of popular downtown beaches. Because the oil also had spread over the shallow seawater intakes for the Vancouver Aquarium, a preliminary screening of samples was performed as a prompt, first response to assess the risks to the Aquarium collection and guide the emergency operational response. A subsequent, more detailed examination for the presence of spilled oil in sediment, biota and water samples from the Vancouver Harbour region was then conducted based on the analysis of a large suite of alkanes, petroleum biomarkers, parent polycyclic aromatic hydrocarbons (PAHs) and alkyl PAH isomers. Most of the commonly applied biomarker ratios exhibit similar values for the spilled oil, Alberta oil (the main petroleum source for British Columbia), and pre-spill and un-oiled sediment samples. In contrast, alkyl PAH isomer ratios showed a clear distinction between the spilled oil and pre-spill samples, with the largest differences shown by isomers of the methyl fluoranthene/pyrene alkyl PAH series. This novel use of alkyl PAH isomers for fingerprinting petroleum helped to confirm the grain carrier MV Marathassa as the source of the oil that affected beach and mussel samples to document definitively the spread of the oil and to establish which samples contained a mix of the oil and hydrocarbons linked to historical activities. Finally, an initial evaluation of the biological risks of the MV Marathassa oil spill in Vancouver Harbour showed that oiled beach sediments had priority parent PAH concentrations that are likely to harm marine life.

Public Event: Commons Governance Workshop: Michel Bauwens of the P2P Foundation

The Global Communication M.A. Double Degree Program is holding Program's Colloquium Presentation - Part III: Commons Governance Workshop with Michel Bauwens of the P2P Foundation on Thursday, March 17, from 10:00am to noon at Vancouver Harbour Center Campus Room 2250. For details, please see the attached PDF.

Alkane and PAH provenance and potential bioavailability in coastal marine sediments subject to a gradient of anthropogenic sources in British Columbia, Canada

Marine coastal areas of British Columbia, Canada are subject to a gradient of increasing anthropogenic sources from pristine areas in the north to the more urbanised areas of the south. Along the gradient, the relative contributions of polycyclic aromatic hydrocarbons (PAHs) from multiple natural and anthropogenic sources are not well understood, but concerns linger about bioavailability and consequent risks to marine biota. Composition patterns and principal components analysis (PCA) models for surface sediment and core samples collected along this transect indicate that samples from the urbanised Vancouver area have the highest proportions of tricyclic terpane, hopane and sterane biomarkers, UCM (unresolved complex mixture), the alkyl fluoranthene/pyrene and benz[a]anthracene/chrysene series and most parent PAHs. Those from Hecate and Queen Charlotte Straits and other locations in the Strait of Georgia have, on the other hand, higher proportions of the resolved alkanes, and petrogenic alkyl naphthalene, fluorene, dibenzothiophene and phenanthrene/anthracene series. PAH ratios clearly indicate a predominance of combustion sources for the four ring and higher parent PAHs for all the sediments. Most samples have values close to the biomass/solid fuel and liquid fuel combustion borderline for ratios of the major PAHs, but liquid fuel combustion input dominates closer to urbanised locations. Ratios also suggest similar pyrogenic and petrogenic inputs between central Hecate Strait and reference sediments from Hecate Strait through to Queen Charlotte Strait, which indicates that biomass combustion and immature coal formations are the major putative respective sources for the reference areas. Alkyl PAH ratios for the C3 and C4 naphthalenes, C2 and C3 phenanthrenes and 3- and 2-methylchrysene, reflecting terrigenous/vascular plant sources and petroleum maturity, exhibit marked differences between Comox bituminous coal and Alberta Sweet Mixed Blend (ASMB) oil and reveal that the petrogenic input in all of the sediments is non-biodegraded. Ratios of these alkyl PAHs with multiple substituents indicate that the sediment samples in the northern Strait of Georgia closest to the Comox coal field receive relatively constant input of eroded coal, while those in the southern Strait of Georgia and Vancouver Harbour show a composition closest to ASMB oil. PAHs associated with liquid fossil fuel combustion and petroleum discharges adjacent to urbanised areas are expected to be fully bioavailable to marine food webs, while the char from biomass combustion and coal from immature deposits in more remote areas would have limited bioavailability. This implies that biota in remote areas of Hecate Strait/Queen Charlotte Sound are likely not adapted to the presence of oil and thus would be more sensitive to exposure to spilled oil than those in the more impacted environments in southern British Columbia.

Guidelines to Prepare for Oil Sands Product Spills in Varied Aquatic Environments

ABSTRACT On July 24, 2007, the Westridge Transfer Line in Burnaby, British Columbia, ruptured spilling 1,400 barrels of oil sands product into the area's storm water systems and eventually into the Burrard Inlet at Vancouver Harbor. The response to this spill was considered successful and there is no record of oil sinking. Several years later, in July of 2010, the Line 6B pipeline operated by Enbridge Energy Partners LLP ruptured spilling 20,082 barrels of oil sands product into the Kalamazoo River. In contrast to the Burnaby spill, this response was extremely difficult due to the sinking of large quantities of oil. The variance in fate and behavior of the oil sands products in these two spills demonstrates how environmental factors can result in different response challenges. Many environmental factors affect the fate of spilled oil sands products in aquatic environments because bitumen, a large component of oil sands products, has a density greater than freshwater. By analyzing specific factors in areas at risk, responders can better prepare for, and expect, submergence in oil sands product spills. Areas identified to have low salinity, rough sedimentation, high turbidity, strong sunlight exposure, high temperatures, and strong currents have a high risk of submergence. Response teams in these areas of high risk should have submerged oil recovery equipment readily available for rapid deployment.

Home site fidelity in Black Rockfish, <em>Sebastes melanops</em>, reintroduced into a fjord environment

Between December 2004 and January 2007, we studied the movements of six Black Rockfish (Sebastes melanops) that had been fitted with acoustic transmitters and reintroduced into a fjord environment at the edge of Vancouver Harbour, British Columbia. The Black Rockfish were released in December 2004 and April 2005 at a reef characterized by complex rocky structures and steep slopes; bottom depths at the site dropped from 10 to 55 m within a horizontal distance of only 30 m. The reef, however, is small (approximately 4500 m2) and is surrounded by soft bottom habitats used infrequently by Black Rockfish. VEMCO VR2 receivers were deployed at the release site and at outlying reefs located 1 and 4 km away. Acoustic data suggest that one individual emigrated from the reef after 11 months of residency and a second individual disappeared abruptly after 6 weeks on the reef, possibly due to fishing mortality or emigration. The four remaining individuals appear to have confined their movements to the release site, using an area 6 to 10 times smaller than the home ranges reported for populations in low-gradient coastlines in California and Oregon.

Properties of Blood, Porphyrins, and Exposure to Legacy and Emerging Persistent Organic Pollutants in Surf Scoters (Melanitta perspicillata) Overwintering on the South Coast of British Columbia, Canada

The surf scoter (Melanitta perspicillata) is a little-studied species of North American sea duck. Estimates suggest it has experienced a precipitous decline in breeding numbers over the latter half of the past century. To investigate the potential role of contaminant uptake and toxicity in the population decline, this study undertook to measure blood chemistry, porphyrin concentrations, EROD, and organic contaminants in mature surf scoters wintering in the Strait of Georgia, BC, Canada. Hepatic organochlorine pesticide, polychlorinated dibenzo-p-dioxin, polychlorinated dibenzofuran, polychlorinated biphenyl (PCB), polybrominated diphenyl ether, and nonylphenol concentrations were relatively low; for example, ΣTEQs (toxic equivalents) for PCBs, dioxins, and furans combined ranged from 4.7 ng/kg wet weight in reference-site (Baynes Sound) birds to 11.4 ng/kg wet weight in birds from Vancouver Harbour. Nonetheless, elevated EROD activity indicated that birds in Howe Sound were responding to an Ah-receptor-mediated stressor, which was also affecting hematocrit values and possibly vitamin A status. In addition, a low proportion of lymphocytes in individuals across locations in early spring samples was associated with poor body condition. The apparent loss of fitness just prior to the onset of northerly migrations to breeding grounds is of particular concern. Compromised health of mature birds at this point in the season might impact negatively on the productivity and survival of some individuals, particularly those overwintering in Howe Sound.

Joined by geochemistry, divided by history: PCBs and PBDEs in Strait of Georgia sediments

Polychlorinated biphenyls (PCBs) are relict contaminants, while polybrominated diphenyl ethers (PBDEs) are in increasing use. Using sediment cores collected in the Strait of Georgia, we demonstrate that the surface sediment concentration of PCBs is largely determined by environmental processes, such as sediment accumulation and mixing rates, while that of PBDEs is strongly influenced by proximity to source. The Iona Island wastewater outfall appears to be a primary pathway for PBDEs. As well, Vancouver Harbour is highly contaminated with both classes of chemical. BDE-209, the main component of deca-BDE, is the dominant PBDE congener. Environmental debromination is not evident. Currently, the ranges of the surface concentration of PCBs and PBDEs are similar to one another, but that will change in the future, as the concentration of PBDEs continues to rise. The experience with PCBs suggests that if PBDEs were banned today, it would take decades for inorganic sediment to bury them.

Dinoflagellate cysts as indicators of water quality and productivity in British Columbia estuarine environments

Palynological analyses of 60 surface sediment samples from estuarine environments near Vancouver Island, including the Georgia Strait (GS), the Effingham (EFF) and the Seymour–Belize (SB) Inlets were performed in order to document the distribution of dinoflagellate cyst assemblages and their relationship to hydrographic conditions, productivity and nutrient concentrations. We tested transfer functions using the analogue method, and suggest that dinoflagellate cyst assemblages can be used to reconstruct primary productivity, temperature and salinity. The EFF and SB Inlets are characterized by a dominance of autotrophic taxa, particularly Operculodinium centrocarpum, whereas the Protoperidinioid and gymnodinial heterotrophic taxa such as Quinquecuspis concreta and Brigantedinium spp. dominate the assemblages of the GS. Multivariate analysis shows that this distribution is closely linked to primary productivity, sea-surface temperature (SST) and spring silica concentration. The abundance of autotrophic taxa in the EFF and SB Inlets is associated with high primary productivity and low summer SST, indicating summer upwelling of coastal British Columbia, whereas the heterotrophic taxa that characterizes the GS assemblages are related to low productivity, high summer SST and high silica concentration during spring. Multivariate analysis shows that the most important environmental parameters related to dinocyst distribution in the restricted embayment of the GS, are distance to the shore, distance to Vancouver Harbor, spring sea surface salinity (SSS), spring phosphate concentration and spring productivity. The autotrophic taxa are generally more common in coastal and shallow waters, but Spiniferites ramosus and Pentapharsodinium dalei show an opposite correlation to spring productivity and salinity. P. dalei is particularly abundant around Vancouver Harbor, near highly urbanized shores and within the Fraser River plume, where salinity is low and spring productivity and continental runoff are high. S. ramosus shows its highest abundance on the western coast of GS. Protoperidinioid and gymnodinial cysts characterize distal zones within the central and southern GS that are associated with a mixture of brackish waters coming from the Fraser River and deep upwelling waters entering the GS via Juan de Fuca Strait. The relationship between dinoflagellate cyst assemblages and primary productivity in these estuarine systems differs from that in oceanic and outer neritic zones, where the abundance of heterotrophic taxa is commonly associated with upwelling and high productivity.

Historical Trends in Mercury Sedimentation and Mixing in the Strait of Georgia, Canada

Seventeen sediment cores collected in the Strait of Georgia reveal a history of mercury contamination that began in the 1860s and include episodic contamination during World War II and in the late 1960s. Surface sediment mercury concentrations ranged from 60 to 420 ng/g dry weight and the current fluxes to sediments are estimated at 5-181 ng cm(-2) a(-1). In one location in Port Moody Arm, a Hg spill seems to have sufficiently poisoned the sediments to eliminate biomixing for about 20 years. Although the surface concentration of Hg is likely to decrease at all stations in coming years, sites in the industrialized Vancouver Harbor and Port Moody Arm will continue to be sufficiently contaminated to endanger benthic organisms. Variations in sedimentation and mixing rates among sites result in surface sediment Hg concentration patterns that do not reflect accurately the distribution of Hg flux. In particular, the concentration of Hg in sediments near the mouth of the Fraser River is low, despite the high Hg load of that river, because of dilution by other particles. A preliminary Hg budget indicates that most of the Hg enters the Strait of Georgia via the Fraser River (2090 kg a(-1)), and that, while burial in Strait of Georgia sediments is a major sink (1800 kg a(-1)), there may be a significant outflow of Hg through Juan de Fuca Strait (approximately 3400 kg a(-1)).

Improving the organisation of breast cancer care increases quality of care while reducing costs

Polychaete taxocene characteristics from seven stations in Vancouver Harbour obtained during the PICES Practical Workshop are presented. Thirty families and 79 polychaete species were identified. Polychaete density ranged from 194.0 individuals m−2 in Port Moody Arm to 846.0 individuals m−2 in the Central Harbour, with a mean abundance over the seven stations of 428.9 individuals m−2. Biomass varied from 3.0 g wet wt. m−2 to 28.5 g wet wt. m−2, with a mean biomass for the seven stations of 12.9 g wet wt. m−2. High species diversity and richness of polychaete taxocene were observed at two stations, the West Vancouver Laboratory in the Outer Harbour and at a far field reference station in Thornborough Cannel. Sediment quality assessment indicated severe adverse effects at two sites in Port Moody Arm, where high concentrations of chlorinated hydrocarbons and some trace metals were found. A limited number of species, dominance of pollution-tolerant Tharyx multifilis, and the lowest values of polychaete biomass and indices of richness and diversity were detected in Port Moody Arm. Rich species composition and domination of pollution-sensitive species provided evidence of unstressed communities at the far field reference station. Sites in the Outer Harbour, Central Harbour and Indian Arm were characterised by intermediate pollutant exposure.

The origin and identity of invertebrate organisms being transported to Canada's Pacific coast by ballast water

We assessed the colonization risk of invertebrate nonindigenous species (NIS) in ballast water being brought into Canada's Pacific coast by indirect and direct methods. Initially we mapped the locations in the northern Pacific Ocean where ships coming into Vancouver Harbour had performed mid-ocean exchange (MOE). Exchange transects (the distance between the start and end positions for the onset and end of MOE) were on average about 400 km long. Samples were also taken from ballast tanks after filling at a northwestern Pacific port and then again after MOE in the mid-Pacific. Invertebrate communities were different pre- and post-MOE, but some coastal organisms were still present after flushing. In addition, samples were obtained from ballast tanks aboard ships in Vancouver Harbour. In "low" salinity samples (<25‰), 13 different taxa were found, and 52 taxa were found in "high" salinity samples (>25‰). Risks could be reduced if MOE was performed in the southerly subtropical domain, south of the subarctic domain and transition zone in the mid-Pacific, or well offshore for north–south shipping routes. For voyages on the west coast of North America, colonization risk of invertebrate NIS is likely higher for ballast water from harbours already colonized by NIS.

Toxicopathic liver lesions in English sole and chemical contaminant exposure in Vancouver Harbour, Canada.

The prevalence of toxicopathic liver lesions in English sole (Pleuronectes vetulus) was determined along a presumed gradient of chemical contamination in Vancouver Harbour, Canada. Fish were captured from five sites in or near Vancouver Harbour, British Columbia, Canada. No toxicopathic lesions were observed in fish examined at the reference site (Howe Sound outside Vancouver Harbour), or at the outer harbour site. In contrast, 20-23% of the fish from three sites located in the central harbour, Indian Arm and Port Moody Arm had one or more types of toxicopathic lesions. Likewise, aromatic hydrocarbon (AH) metabolites measured in bile exhibited a gradient in levels from lower concentrations at the reference site to significantly higher levels in fish from Indian Arm and Port Moody Arm harbour sites. The occurrence of toxicopathic liver lesions was statistically associated with concentrations of AHs measured in sediment and AH metabolite levels measured in bile.

Organic and trace metal contaminants in sediments and English sole tissues from Vancouver Harbour, Canada.

As part of a multinational workshop on marine environmental quality, sediments were collected from seven sites in Vancouver Harbour and analyzed for polynuclear aromatic hydrocarbons (PAHs) and organochlorines (OCs), and for trace metals. English sole were collected from five sites, and muscle was analyzed for trace metals and liver for OCs. As expected, sediment PAH and OC concentrations and tissue OC concentrations were higher at sites east of the First Narrows, compared to the outer harbor and reference sites. Sediment PAH concentrations east of the First Narrows were similar to concentrations at moderately contaminated sites in Puget Sound, south of Vancouver Harbour. In contrast, concentrations of OCs in sediments and tissue were low to moderate, even at relatively contaminated sites within Vancouver Harbour. Although several trace metals in sediments were higher than in contaminated sediments from Puget Sound, trace metals measured in fish muscle were lower.

Hepatic CYP1A levels and EROD activity in English sole: biomonitoring of marine contaminants in Vancouver Harbour.

To assess chemical contaminant stress in the marine environment, ethoxyresorufin-O-deethylase (EROD) activity and cytochrome P450 1A (CYP1A) expression were measured in 88 English Sole (Pleuronectes vetulus) collected during May and June 1999 from four sites in Vancouver Harbour and at an expected reference site outside the harbour. Hepatic microsomes were prepared from the fish and analyzed for total CYP content, EROD activity, and CYP1A protein levels. Hepatic EROD activity and CYP1A protein levels were elevated in fish from two sites in the inner harbour. A comparison with sediment chemistry data showed that fish with increased EROD activity and CYP1A levels came from sites containing relatively high levels of polycyclic aromatic hydrocarbons and polychlorinated biphenyls. Unexpectedly high levels of EROD activity and CYP1A protein were also found in fish from a reference site near Gibsons, in Howe Sound. The elevated EROD activity and CYP1A expression in fish from this site cannot be explained by the chemical analysis data collected.

Introduction to the PICES practical workshop: objectives, overview of the study area, and projects conducted by the participants.

We coordinated a collaborative research project to investigate environmental conditions in Vancouver Harbour, British Columbia, Canada, between 23 May and 7 June 1999. This special volume of Marine Environmental Research presents a collection of papers giving results of these studies. The project was part of a practical workshop sponsored by the Marine Environmental Quality (MEQ) committee of the North Pacific Marine Science Organization (PICES). Twenty-four scientists from PICES countries (USA, Canada, Russia, Japan, Korea, and China) participated. A wide variety of data was collected, including community structure of benthic invertebrates and fish, evaluation of fish health using biological markers and exposure data, evaluation of contaminant exposure in inter-tidal invertebrates, imposex in gastropods, and information about natural toxins produced by algae. The workshop provided an opportunity for PICES participants to gain an improved appreciation of the approaches and techniques used by other member countries to assess the effects of marine pollution. The purpose of this introductory paper is to briefly describe the project, provide background information on how it was organized, and give an overview of our knowledge about the harbour's environment.