Conrail, Consolidated Rail Corporation, founded in 1976, was established due to the bankruptcy of multiple railway companies in the Northeast and Midwest regions of the United States. The federal government intervened to establish the company with the aim of restoring the services and profitability of bankrupt railways. As it developed, Conrail's railway network covered major cities and industrial centers in the Northeast and Midwest of the United States, possessing a strong market position and strategic value. Therefore, Conrail had become a target for mergers and acquisitions by large railway companies such as CSX and Norfolk Southern. Through mergers and acquisitions, the acquiring party could expand market coverage, enhance operational network connectivity and competitiveness. This is a complex and multi-layered issue, the CSX acquisition of Conrail involved financial and market implications, legal obstacles, competitive pressures, and strategic objectives. By analyzing these themes in depth, we could understand the key issues and response strategies that enterprises face in the M&A process, providing valuable experience for future M&A transactions. The study applied methods such as comparative analysis and case model analysis to analyze the main challenges, response strategies, and effects comprehensively and systematically in the M&A process, providing reference for similar cases. Overall, CSX's acquisition of Conrail demonstrates how companies can achieve acquisition goals and long-term development through flexible strategies, effective communication, and professional management in the face of complex legal, market, and operational challenges.