Valley Population Research Articles

The Characterization of the Morphological and Molecular Traits of Phaseolus coccineus in the Aniene Valley: Insights into Genetic Diversity and Adaptation

This study aimed to characterize the genetic diversity and morphological traits of 32 populations of Phaseolus coccineus collected from the Aniene Valley. Quantitative seed trait analyses revealed that Mandolone accessions exhibited significantly higher seed weights and dimensions compared to Fagiolone accessions. Specifically, Mandolone accessions showed an average weight of 189.48 g per 100 seeds, with seed heights and lengths averaging 14.38 mm and 23.84 mm, respectively. In contrast, Fagiolone accessions had an average seed weight of 174.13 g, with seed heights and lengths of 13.95 mm and 22.58 mm, respectively. Molecular analysis, conducted using 9 polymorphic SSR loci, identified 53 alleles across 320 genotypes, with a mean expected heterozygosity (He) of 0.695. This indicated that there was high genetic variability within the Aniene Valley populations. The genetic diversity analysis revealed two genetic clusters corresponding to the Mandolone and Fagiolone landraces. This was supported by UPGMA, PCoA, and STRUCTURE analyses. This study highlights the need to conserve the genetic diversity within these landraces and provides a basis for the development of conservation strategies for P. coccineus germplasms in the singular ecological context of the Aniene Valley.

Population growth poses a significant threat to forest ecosystems: A case study from the Hindukush-Himalayas of Pakistan.

Human population growth and the accompanying increase in anthropogenic activities pose a significant threat to forest ecosystems by reducing the natural services these ecosystems provide. Malam Jabba, located in the District Swat of Pakistan's Hindukush-Himalayan temperate zone, is known for its ecotourism, skiing, timber-producing tree species, medicinal plants, and unique biodiversity. However, a large portion of Swat Valley's population depends on the Malam Jabba forests for timber and fuelwood. This study investigates how deforestation rates have increased in response to the growing human population in Malam Jabba, District Swat. To monitor forest cover changes, we used remote sensing (RS) and geographic information systems (GIS) tools. Vegetation analysis was conducted using the Normalized Difference Vegetation Index (NDVI) based on multi-temporal satellite imagery from 1980, 2000, and 2020. Using a decay model, we calculated the deforestation rate from 1980 to 2020 and projected future rates using MATLAB, based on anticipated population growth. Our results show that over the last two decades, the average annual deforestation rate rose from 0.7% to 1.93%, coinciding with a population increase from 1.2 million to 2.3 million at a growth rate of 9% per year. Projections indicate that the deforestation rate will increase to 2.5% annually over the next 20 years, given the predicted 11.6% yearly population growth. Population growth in District Swat has severely endangered nearby forest ecosystems, and further increases in human activity, such as unsustainable tourism, fuel and timber collection, and urbanization, will likely exacerbate this trend. Based on our findings, we recommend: (i) the implementation of reforestation programs and sustainable forest resource use; (ii) the development of a long-term forest management plan that maintains equilibrium between forest density and population pressure; and (iii) prioritizing areas with extreme human impact for in-situ conservation efforts.

Isolation of Shewanella algae from blue crabs (Callinectes sapidus) in Northwestern Adriatic Sea

Abstract Blue crab (Callinectes sapidus) is one of the last reported invasive species that have colonized the Italian coasts, in particular the lagoons of the northern Adriatic Sea. Their massive presence is compromising shellfish farming, causing a serious economic crisis among the valley populations. To limit the economic damage, blue crabs have been introduced as human food in the Italian markets. This study aimed to evaluate the microbiological contamination of blue crab as potential risk to human health. From July 2023 to April 2024, 72 blue crabs were collected in coastal lagoons and 5-6 nm from the coast of the Emilia Romagna Region. Microbiological analysis revealed Shewanella algae as one of the most commonly identified microorganisms (18/72, 25%). Shewanella was isolated on Chromagar Vibrio™ and TCBS after an enrichment step in Alkaline Saline Peptone Water. Colony morphology, Gram stain, and biochemical tests (oxidase, catalase) sustained Shewanella identification, later confirmed by MALDI-TOF MS (MALDI Biotyper® Sirius, Bruker Daltonics). S. algae is a Gram-negative, rod-shaped, aerobic microorganism widely distributed in seawater, freshwater, and soil. In humans, it is considered an opportunistic pathogen, especially in people with impaired immune systems. Shewanellosis has been strongly associated with seawater exposure and typically arises in hot months. Clinical manifestations include skin and soft tissue infections, otitis media, keratitis, biliary tract infections, vertebral discitis, and bloodstream infections. Notably, in the last years, S. algae infections have increased globally, even in healthy young people. The present study suggests blue crabs as a potential source of S. algae throughout the year. The increasing identification of S. algae in marine waters worldwide, as well as in marine-derived foods like blue crabs, underscores the growing importance of S. algae as an emerging pathogen and its impact on public health. Key messages • Shewanella algae is an opportunistic pathogen, increasingly isolated in marine water and seafood. • Blue crab is an invasive species that has recently conolonised the Italian coasts.

Exploring galaxy properties of eCALIFA with contrastive learning

Contrastive learning (CL) has emerged as a potent tool for building meaningful latent representations of galaxy properties across a broad spectrum of wavelengths, ranging from optical and infrared to radio frequencies. These latent representations facilitate a variety of downstream tasks, including galaxy classification, similarity searches in extensive datasets, and parameter estimation, which is why they are often referred to as foundation models for galaxies. In this study, we employ CL on the latest extended data release from the Calar Alto Legacy Integral Field Area (CALIFA) survey, which encompasses a total of 895 galaxies with enhanced spatial resolution that reaches the limits imposed by natural seeing (FWHMPSF ∼ 1.5). We demonstrate that CL can be effectively applied to Integral Field Unit (IFU) surveys, even with relatively small training sets, to construct meaningful embedding where galaxies are well separated based on their physical properties. We discover that the strongest correlations in the embedding space are observed with the equivalent width of Hα, galaxy morphology, stellar metallicity, luminosity-weighted age, stellar surface mass density, the [NII]/Hα ratio, and stellar mass, in descending order of correlation strength. Additionally, we illustrate the feasibility of unsupervised separation of galaxy populations along the star formation main sequence, successfully identifying the blue cloud and the red sequence in a two-cluster scenario, and the green valley population in a three-cluster scenario. Our findings indicate that galaxy luminosity profiles have minimal impact on the construction of the embedding space, suggesting that morphology and spectral features play a more significant role in distinguishing between galaxy populations. Moreover, we explore the use of CL for detecting variations in galaxy population distributions across different large-scale structures, including voids, clusters, and filaments and walls. Nonetheless, we acknowledge the limitations of the CL framework and our specific training set in detecting subtle differences in galaxy properties, such as the presence of an AGN or other minor scale variations that exceed the scope of primary parameters such as the stellar mass or morphology. Conclusively, we propose that CL can serve as an embedding function for the development of larger models capable of integrating data from multiple datasets, thereby advancing the construction of more comprehensive foundation models for galaxies.

Effects of strain-tunable valleys on charge transport in bismuth

The manipulation of the valley degree of freedom can boost the technological development of novel functional devices based on valleytronics. The current mainstream platform for valleytronics is to produce a monolayer with inversion asymmetry, in which the strain-band engineering through the substrates can serve to improve the performance of valley-based devices. However, pinpointing the effective role of strain is inevitable for the precise design of the desired valley structure. Here, we demonstrate the charge transport under continuously controllable external strain for bulk bismuth crystals with three equivalent electron valleys and one hole valley. The strain response of resistance, namely elastoresistance, exhibits evolutions in both antisymmetric and symmetric channels with decreasing temperature. The elastoresistance behaviors mainly reflect the significant changes in valley density depending on the symmetry of induced strain, evidenced by our strain-dependent quantum oscillation measurements and first-principles band calculations under strain. These facts suggest the successful tuning and evaluation of the valley populations through strain-dependent charge valley transport. Published by the American Physical Society 2024

Genetic assessment and monitoring of wild, captive, and reintroduced northern leopard frog populations

The northern leopard frog (Lithobates pipiens) has undergone dramatic declines in population size and range over recent decades in western Canada and the United States. In British Columbia, only a single population remains at the Creston Valley Wildlife Management Area. Yet, the continuing viability of this population is uncertain. In this paper, the current genetic structure of northern leopard frog populations in western Canada was assessed using microsatellite markers. Historical samples from the extinct population of Fort Steele in British Columbia were compared with the Creston Valley population to understand changes in population genetic parameters over time. Genotypic data from four populations (Creston Valley, Drain K, Prince Spring, and Cypress Hill) sampled in 2004 and 2019 were compared. To evaluate changes in the genetic diversity of the Creston Valley population over time, allelic richness and expected heterozygosity of the population were compared at three time points using genotypes from 2000, 2004, and 2019. Northern leopard frog populations in western Canada showed high genetic differentiation, with genetic diversity decreasing from east to west. Although there weren’t notable changes in genetic parameters between 2004 and 2019, there was evidence of a decline in diversity between 2000 and 2019. The extinct population of Fort Steele had private alleles, while the current Creston Valley population did not, suggesting a genetic bottleneck in the Creston Valley population. Therefore, genetic rescue, specifically for the endangered Creston Valley population, can be considered as an action to support recovery. Additionally, continued genetic monitoring will help in the effective management of the species by providing information on the success of conservation actions.

Nonlinear Current Injection in Hexagonal Boron Nitride using Linearly Polarized Light in a Deeply Off‐Resonant Regime

AbstractLight‐induced electron dynamics in monolayer hexagonal boron nitride is theoretically investigated under the influence of two‐color linearly‐polarized laser fields at frequencies ω and 2ω, by solving the time‐dependent Schrödinger equation with a tight‐binding model. In the weak field regime, it is confirm that the injection of ballistic current arises from the breakdown of time‐reversal symmetry. This phenomenon is attributed to quantum interference between two distinct excitation paths: a one‐photon (2ℏω) absorption path and a two‐photon (ℏω) absorption path. In a strong field regime, the analysis reveals that the two‐color laser fields may generate a substantial population imbalance within momentum space, consequently facilitating the injection of ballistic current even in a deeply off‐resonant regime. The findings demonstrate that a pronounced population imbalance exceeding 30% of excited electrons can be realized without relying on the ellipticity of the fields. This highlights the potential of linearly polarized light for efficient photovoltaic effects and valley population control in 2D systems and heterostructures.

From hunter-gatherers to food producers: New dental insights into the Nile Valley population history (Late Paleolithic-Neolithic).

This study presents biological affinities between the last hunter-fisher-gatherers and first food-producing societies from the Nile Valley. We investigate odontometric and dental tissue proportion changes between these populations from the Middle Nile Valley and acknowledge the biological processes behind them. Dental remains of 329 individuals from Nubia and Central Sudan that date from the Late Pleistocene to the mid-Holocene are studied. Using 3D imaging techniques, we investigated outer and inner metric aspects of upper central incisors, and first and second upper molars. Late Paleolithic and Mesolithic foragers display homogeneous crown dimensions, dental tissue proportions, and enamel thickness distribution. This contrasts with Neolithic trends for significant differences from earlier samples on inner and outer aspects. Finally, within the Neolithic sample differences are found between Nubian and Central Sudanese sites. Substantial dental variation appears to have occurred around 6000 bce in the Nile Valley, coinciding with the emergence of food-producing societies in the region. Archeological and biological records suggest little differences in dietary habits and dental health during this transition. Furthermore, the substantial variations identified here would have happened in an extremely short time, a few centuries at most. This does not support in situ diet-related adaptation. Rather, we suggest these data are consistent with some level of population discontinuity between the Mesolithic and Neolithic samples considered here. Complex settlement processes could also explain the differences between Nubia and Central Sudan, and with previous results based on nonmetric traits.

Species divergence in valleys: the phylogeny of Phrynocephalus forsythii complex and description of a new species.

Geographic isolation caused by high-altitude valleys promotes the formation of geographic segregation of species, leading to species differentiation. The subgenus Oreosaura contains viviparous species from the Tibetan Plateau and the vicinity of the Tarim Basin, which can be divided into three species complexes according to their geographical distribution: Phrynocephalus vlangalii, Phrynocephalus theobaldi, and Phrynocephalus forsythii. However, molecular data for the P. forsythii complex are limited and the diversity of this species complex has been greatly underestimated. Therefore, this study aimed to explore the species diversity of Oreosaura and species differentiation within the P. forsythii complex. We analysed the species diversity of Oreosaura by combining previous data, constructed a phylogenetic tree of the subgenus based on cytochrome c oxidase subunit I and 16S sequences, and estimated the divergence time. The results suggest significant genetic differences between the Tarim Basin populations and adjacent mountain valley populations of the P. forsythii complex and that the combination of deep valley landscapes in the high mountains and ice-age events have contributed to the differentiation of the viviparous toad-headed agama lizard, which is a key factor in the phylogenetics of the P. forsythii complex. Furthermore, we identified a population collected from Wuqia County, Xinjiang, as a new species, Phrynocephalus kangsuensis sp. nov. The results will provide data for phylogenetic studies following the P. forsythii complex and help demonstrate that valleys promote the formation of Phrynocephalus species.

Patrimonio histórico del valle bajo del río Chillón. Investigación y educación

This article examines approaches the cultural heritage characteristics of the Lower Chillón River Valley populations, from a multidisciplinary perspective: archaeology, history, architecture and education. In order to accomplish such goal, it is discussed the history and current state of the Pre-Hispanic, Colonial and Republican cultural legacy of the studied area, along with its conservation and implementation of educational programs for the local community.

Light-Shaping of Valley States.

The established paradigm to create valley states, excitations at local band extrema ("valleys"), is through selective occupation of specific valleys via circularly polarized laser pulses. Here we show a second way exists to create valley states, not by valley population imbalance but by "light-shaping" in momentum space, i.e. controlling the shape of the distribution of excited charge at each valley. While noncontrasting in valley charge, such valley states are instead characterized by a valley current, identically zero at one valley and finite and large at the other. We demonstrate that these (i) are robust to quantum decoherence, (ii) allow lossless toggling of the valley state with successive femtosecond laser pulses, and (iii) permit valley contrasting excitation both with and without a gap. Our findings open a route to robust ultrafast and switchable valleytronics in a wide scope of 2d materials, bringing closer the promise of valley-based electronics.

Seedling growth and spinescence of three Vachellia species' populations in valleys formed by tectonic uplift

AbstractThe Southeastern Coastal Hinterland geomorphic province of South Africa is dissected by deep valleys created by tectonic uplift in the early Miocene and late Miocene/early Pliocene. We hypothesised that seedlings of Vachellia nilotica, V. tortilis and V. karroo grown from seeds from different valleys would differ in growth and spinescence because of geographical separation. Ninety seedlings (30 per species) were grown for 3 months in uniform conditions in a greenhouse. Mean height of V. nilotica and V. tortilis seedlings was greatest for the Phongolo populations and lowest for the Thukela populations. Maximum stem diameter of both species was attained by individuals from the Phongolo population. Mean spine dry mass of V. nilotica seedlings was greatest for the Phongolo population, suggesting a growth‐defence trade‐off, but did not differ for V. tortilis. Results suggested that Phongolo populations of V. nilotica and V. tortilis may be characterised by morphotypes clearly distinct from those in the other valleys. Unclear and inconsistent results for V. karroo in this study suggested subtly different morphotypes occur in each valley. We propose that distinct Phongolo morphotypes of V. nilotica and V. tortilis are explained as evolutionary adaptations to moderate fire and high browsing pressure compared to populations South of Phongolo.

Maternal ancestry of first Parsi settlers of India using ancient mitogenome

The rich cultural and genetic diversity of South Asia emerged from multiple migrations and cultural assimilation of multiple waves of migrants. The Parsi community of North-western India were one of those who migrated from West Eurasia in the aftermath of 7th century CE and assimilated into the local cultural framework. Earlier genetic studies further strengthened this notion with the finding that they harbour both Middle Eastern and South Asian genetic components. Although these studies covered both autosomal and uniparental markers, still maternal ancestry was not covered in depth and with good resolution of mitochondrial markers. Hence in our current study, we have first time generated a complete mitogenome of 19 ancient samples of the first Parsi settlers excavated from the archaeological site of Sanjan and performed detailed phylogenetic analysis to infer their maternal genetic affinity. In our analysis, we found that the Parsi mitogenome with mtDNA haplogroup M3a1 + 204 shares clade with both Middle Eastern and South Asian modern individuals in both the Maximum Likelihood tree and Bayesian phylogenetic tree. This haplogroup was also prevalent among the medieval Swat valley population of present-day Northern Pakistan and was also observed in two Roopkund A individuals. In the phylogenetic network this sample share haplotype with both South Asian and Middle Eastern samples. So conclusively, the first Parsi settlers’ maternal ancestry encompasses both South Asian and Middle Eastern genetic composition.

Nonlinear All-Optical Coherent Generation and Read-Out of Valleys in Atomically Thin Semiconductors.

With conventional electronics reaching performance and size boundaries, all-optical processes have emerged as ideal building blocks for high speed and low power consumption devices. A promising approach in this direction is provided by valleytronics in atomically thin semiconductors, where light-matter interaction allows to write, store, and read binary information into the two energetically degenerate but non-equivalent valleys. Here, nonlinear valleytronics in monolayer WSe2 is investigated and show that an individual ultrashort pulse with a photon energy tuned to half of the optical band-gap can be used to simultaneously excite (by coherent optical Stark shift) and detect (by a rotation in the polarization of the emitted second harmonic) the valley population.

Risk Assessment of Rising Temperatures Using Landsat 4–9 LST Time Series and Meta® Population Dataset: An Application in Aosta Valley, NW Italy

Earth observation data have assumed a key role in environmental monitoring, as well as in risk assessment. Rising temperatures and consequently heat waves due to ongoing climate change represent an important risk considering the population, as well as animals, exposed. This study was focused on the Aosta Valley Region in NW Italy. To assess population exposure to these patterns, the following datasets have been considered: (1) HDX Meta population dataset refined and updated in order to map population distribution and its features; (2) Landsat collection (missions 4 to 9) from 1984 to 2022 obtained and calibrated in Google Earth Engine to model LST trends. A pixel-based analysis was performed considering Aosta Valley settlements and relative population distribution according to the Meta population dataset. From Landsat data, LST trends were modelled. The LST gains computed were used to produce risk exposure maps considering the population distribution and structure (such as ages, gender, etc.). To check the consistency and quality of the HDX population dataset, MAE was computed considering the ISTAT population dataset at the municipality level. Exposure-risk maps were finally realized adopting two different approaches. The first one considers only LST gain maximum by performing an ISODATA unsupervised classification clustering in which the separability of each class obtained and was checked by computing the Jeffries–Matusita (J-M) distances. The second one was to map the rising temperature exposure by developing and performing a risk geo-analysis. In this last case the input parameters considered were defined after performing a multivariate regression in which LST maximum was correlated and tested considering (a) Fractional Vegetation Cover (FVC), (b) Quote, (c) Slope, (d) Aspect, (e) Potential Incoming Solar Radiation (mean sunlight duration in the meteorological summer season), and (f) LST gain mean. Results show a steeper increase in LST maximum trend, especially in the bottom valley municipalities, and especially in new built-up areas, where more than 60% of the Aosta Valley population and domestic animals live and where a high exposure has been detected and mapped with both approaches performed. Maps produced may help the local planners and the civil protection services to face global warming from a One Health perspective.

Ultrafast Dynamics of Valley-Polarized Excitons in WSe2 Monolayer Studied by Few-Cycle Laser Pulses.

We report on the experimental investigation of the ultrafast dynamics of valley-polarized excitons in monolayer WSe2 using transient reflection spectroscopy with few-cycle laser pulses with 7 fs duration. We observe that at room temperature, the anisotropic valley population of excitons decays on two different timescales. The shorter decay time of approximately 120 fs is related to the initial hot exciton relaxation related to the fast direct recombination of excitons from the radiative zone, while the slower picosecond dynamics corresponds to valley depolarization induced by Coloumb exchange-driven transitions of excitons between two inequivalent valleys.

Swainson’s Hawk Nesting Population in the Antelope Valley of the Western Mojave Desert, California

The Swainson’s Hawk (Buteo swainsoni) has a long history of breeding in California, but a severe decline in the statewide breeding population was identified in 1979, when in all of southern California only two pairs were found, one in the Antelope Valley of the western Mojave Desert. That area was little studied until we began banding Swainson’s Hawks there in 1997. Over 20 breeding seasons between 1979 and 2022, we documented in the Antelope Valley 124 attempts to nest, in which the mean clutch and brood sizes were 2.49 and 2.37, respectively. From 2004 through 2006, we observed two to four breeding pairs annually; from 2009 through 2022, three to 14 breeding pairs. The rate of success of the 91 nests revisited to determine if any young fledged was 64%. Nest trees consisted of 81.5% non-native species, 13.7% native species, including Joshua trees (Yucca brevifolia), and 4.8% unidentified deciduous trees. Between 1997 and 2022, in 50 nests, we recorded 170 vertebrate prey items, of which 90 were gophers (Thomomys bottae). Though the Antelope Valley population has grown since 1980, its nesting and foraging habitat now face multiple threats. To conserve occupied nesting territories, we recommend creation of nesting and foraging habitat reserves that include both native desert and cultivated alfalfa close to existing conserved land.

Selenium in drinking water and cereal grains, and biomarkers of Se status in urine and fingernails of the Main Ethiopian Rift Valley population

BackgroundSelenium (Se) plays an important role in human health, yet Se overexposure or deficiency can lead to deleterious health effects. This study aims to determine the concentration of Se in drinking water and staple cereal grain (maize, wheat, and teff) samples from the Main Ethiopian Rift (MER) Valley, and correspondingly, assesses Se biomarkers and their status as measured in the urine and fingernails of 230 individuals living in 25 MER communities. MethodThe concentration of Se in drinking water and cereal grain (maize, wheat, and teff) samples, and urine and fingernail samples were measured using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Demographic, anthropometric, and elemental concentrations were described by their quartiles and mean ± standard deviations. The 5th and 95th percentiles were used to describe the concentrations Se biomarkers ranges. The Se biomarker distributions in different study communities were further characterized according to Se levels found in drinking water, sex, and age using ANOVA, and multivariate regression. We conducted a correlation analysis (with Pearson correlation coefficient) and fitted a regression to evaluate the associations between these variables. ResultsThe mean concentration of Se in the drinking water samples was 0.66 (range: 0.015–2.64 µg/L; n = 25), and all samples were below the threshold value of 10 μg/L for Se in drinking water set by the World Health Organiation (WHO). In Ethiopia, most rural communities rely on locally produced cereal grains. We found mean Se concentrations (µg/kg) of 357 ± 190 (n = 14), 289 ± 123 (n = 14), and 145 ± 100 (n = 14) in wheat, teff, and maize, respectively. Furthermore, Se concentrations in drinking water showed no significant correlation with biomarker measures, indicating that the primary source of dietary Se is likely from local foods including staple grains. The mean±SD (5th–95th percentiles) of Se concentrations in fingernails and urine among study subjects were 1022 ± 320 (624–1551 µg/kg), and 38 ± 30 (1.9–100 µg/L), respectively. ConclusionA sizeable share of study participants (31%) fell below the lower limits of what is considered the currently accepted Se range of 20–90 µg/L in urine, though relatively few (only 4%) had similarly low fingernail levels. On the other hand, none of the samples reached Se toxicity levels, and the biomarker levels in this study are comparable to results from other studies that find adequate Se. Our results show that Se toxicity or deficiency is unlikely in the study population.

Spatio-Temporal Evolution of Glacial Lakes in the Tibetan Plateau over the Past 30 Years

As the Third Pole of the Earth and the Water Tower of Asia, the Tibetan Plateau (TP) nurtures large numbers of glacial lakes, which are sensitive to global climate change. These lakes modulate the freshwater ecosystem in the region but concurrently pose severe threats to the valley population by means of sudden glacial lake outbursts and consequent floods (GLOFs). The lack of high-resolution multi-temporal inventory of glacial lakes in TP hampers a better understanding and prediction of the future trend and risk of glacial lakes. Here, we created a multi-temporal inventory of glacial lakes in TP using a 30-year record of 42,833 satellite images (1990–2019), and we discussed their characteristics and spatio-temporal evolution over the years. Results showed that their number and area had increased by 3285 and 258.82 km2 in the last 3 decades, respectively. We noticed that different regions of the TP exhibited varying change rates in glacial lake size; most regions show a trend of expansion and increase in glacial lakes, while some regions show a trend of decreasing such as the western Pamir and the eastern Hindu Kush. The mapping uncertainty is about 17.5%, which is lower than other available datasets, thus making our inventory reliable for the spatio-temporal evolution analysis of glacial lakes in the TP. Our lake inventory data are publicly published, it can help to study climate change–glacier–glacial lake–GLOF interactions in the Third Pole and serve as input to various hydro-climatic studies.

Phylogenetic and morphological analysis of Gloydius himalayanus (Serpentes, Viperidae, Crotalinae), with the description of a new species

Gloydius is a widespread pitviper group occurring from Eastern Europe to Korea and Siberia, with only one known species, G. himalayanus (Günther, 1864), found south of the Himalayas. We provide combined genetic and morphological data for G. himalayanus from specimens collected from Himachal Pradesh, India. Bayesian Inference and Maximum Likelihood phylogenetic analysis were performed on four concatenated mitochondrial genes, along with a multi-locus coalescent analysis of these and five additional nuclear genes. Our results indicate that G. himalayanus from the Chamba Valley, in western Himachal Pradesh, are highly distinct from the remaining studied populations. Haplotype networks of each nuclear locus showed that G. himalayanus contains high haplotype diversity with low haplotype sharing between the Chamba Valley population and populations from further west. Principal component analysis and canonical variate analysis conducted on morphological data of live and museum specimens also highlight the morphological distinctiveness of the Chamba population and we herein describe this population as a new species, Gloydius chambensis sp. nov. Recent descriptions of other new species of snakes from this valley underscores its isolation and suggests that further herpetological investigation of the highly dissected landscapes of the western Himalayas is needed to assess the true diversity of the region.