Abstract The Eurasian eagle-owl (Bubo bubo) has adapted to hunt local fauna that are subject to the influence of human agricultural activities. In the sparse forests, marshes and lakes of the mountainous regions of central Norway, the eagle-owl’s diet is dominated by small mammals and gallinaceous birds, but the common frog Rana temporaria features more frequently in regions near the fjords, with various species of seabirds predominating on some islands. The eagle-owls, breeding in several protected regions of the Czech Republic, hunting in agricultural areas small mammals and game animals in addition to waterbirds nesting by fishponds. In Slovakia, the majority of the eagle-owl population nests on the edges of mountain valleys. In the past, they hunted small mammals and amphibians on pastureland located deep in the mountains, but they have adapted to hunting larger prey in more intensively farmed valleys. Predominantly in the eastern part of Turkey, the diet of Eurasian eagle-owl hunting on natural mountain steppes has been studied, where mammals of the family Cricetidae dominate. In the arid conditions of the southern countries of Syria, Jordan and Israel, mammals of the Gerbillinae sub-family predominated in addition to a higher proportion of birds. In the more variable areas of Iran, eagle-owl diets feature a wide range of indigenous mammals and birds, with different species represented in several territories. Studies from the edges of the Fergana Valley in southern Kyrgyzstan found differences in the diets of eagle-owls living at lower elevations and those living higher in the mountains, while birds were the predominant prey in the Kalek site. Smaller samples of eagle-owl diets were also collected in the Altai Mountains of Mongolia, the Barguzinsky Mountains to the east of Lake Baikal and the Vaida Mountain on Sakhalin Island. The results presented in this study can also contribute to our knowledge about the fauna of the above-mentioned regions.