The aim of this paper is to evaluate the position of the Holocene transgression on the coast of the Valencia Gulf. To achieve this goal, a sedimentological and micropaleontological analysis of samples of fossil benthic foraminifera in cores from six boreholes drilled in three different wetlands (Almenara marsh, Moro marsh and Valencia lagoon) was carried out. In order to assess the extent of sea level change, at least three factors must be taken into account: 1) eustatic rise (global scale) 2) neotectonics (regional scale) and 3) the sedimentary factor (local and regional scale). Regarding the first factor, some authors have noted that the Holocene transgression reached +1 m above the current sea level in this area. As for the second factor, while the Valencia lagoon is located on a subsiding coast the northernmost marshes are located on a tectonically stable coast. Finally, sedimentation rates are highly variable depending on the area; whereas in the Valencia lagoon the shoreline generally shifts seawards (progradation) in the northernmost areas the coast is currently undergoing retrogradation, although the anthropic factor clearly interferes with this pattern. One of the main conclusions is that in the Valencian coast the Holocene maximum relative sea level did not exceed the present-day one, except in the case of the subsiding area of the Valencia lagoon. The calculation of sedimentation rates, the relative sea-level curve and the evolution of the water column in the different wetlands seem to corroborate it. Radiocarbon and AAR analyses allowed us to date the maximum extent of the Holocene transgression on the Valencian coast at around 5500 cal yr BP in all cases. Despite the different subsidence the three studied wetlands reflected the predominant marine influence until 5500–5200 BP, which was later followed by a growing fluvial or continental incidence, when glacioeustatic adjustments defined the patterns of coastal progradation.