Vacuum Containers Research Articles

Clinical efficacy of titanium prepared platelet rich fibrin in periodontal regeneration: A systematic review and meta-analysis.

Periodontal regeneration therapies frequently involve autologous platelet concentrates (APCs). They can be used in sinus lift surgeries and socket preservation, among other clinical settings. Platelet rich fibrin (PRF) membrane has been used to treat gingival recession in individuals or groups of individuals using a coronally progressed or lateral pedicle flap. In the treatment of mixed periodontic endodontic lesion/furcation defect, PRF functions as a healing and interpositional biomaterial, filling a cystic cavity. PRF is known to help the bone regeneration process. In the last few years, efforts have been made to enhance the PRFs characteristics and quality. One of them is titanium platelet rich fibrin (T-PRF). Third-generation platelet concentrate no longer contains silica, and its preparation in glass vacuum containers, that no longer creates any known concerns. The effectiveness PRF's has been evaluated in connective tissue and bone repair. The aim of this study is to compare T-PRF to other platelet concentrates and different treatment modalities for periodontal regenerative procedures. A protocol of this systematic review have been registered in prospero (CRD42022293545). The online database searched were PUBMED, COCHRANE for published articles up to November 2022 without language restrictions. Studies in trial registers, handsearching, bibliographic references of relevant articles were also checked. Data collection and analysis was done by individual authors. Independent eligibility assessments were conducted by four review authors. Then, using the standard Cochrane methodology, four review authors extracted the data and evaluated the risk of bias for individual studies. We developed "Summary of findings" tables and used GRADE to evaluate the evidence. Three studies were included for meta-analysis. Results of meta-analysis supported that T-PRF is effective for correction of both hard and soft tissue defects. The overall qualitative and quantitative analysis suggest that T-PRF has superior structural properties and thicker fibrin network for ensuring predictable success periodontal regenerative procedures.

Comparison of the results from light and automated microscopic examination of urine sediment obtained using vacuum containers with conical and round bottoms

Comparison of the results from light and automated microscopic examination of urine sediment obtained using vacuum containers with conical and round bottoms

Prospective comparison between a peristaltic pump and vacuum containers for paracentesis: Time, resources and safety

Rationale and objectivesTo meet the increasing demand for radiology departments to perform paracenteses, this study was done to compare the operational, financial and clinical impact of draining ascites with a peristaltic pump versus conventional vacuum containers. Materials & methodsProspective cohort study of 157 paracenteses (56 subjects) drained with ACCEL® evacuated drainage bottles (B. Braun Interventional Systems, Bethlehem, PA) and 159 paracenteses (53 subjects) drained with the RenovaRP® pump (Laborie Medical Technologies Corp., Portsmouth, NH). A short elective questionnaire was then distributed to the procedure staff and the subjects drained by both methods. ResultsMean volume drained with the pump (5 L) was comparable to that drained by vacuum containers (4.9 L, p = 0.77). Mean time to drain subjects with the pump (18.6 min) was 9.1 min shorter and 3.8 min less variable than subjects drained with vacuum containers (27.7 min). This difference was statistically significant (p < 0.01) and clinically important (effect size = 0.73). Flow rate with the pump (4 min/L) was significantly faster (p < 0.05) than vacuum containers flow rate (6.6 min/L). No adverse events occurred in either group. Use of the pump increased the average cost by 21% and reduced earnings by 3%. All assistants (n = 6) and patients (n = 10) that responded to the questionnaire recommended the use of the pump over vacuum containers. ConclusionThe peristaltic pump safely drains ascites significantly faster and with less variability in time than vacuum containers. While use of the pump slightly increases cost per paracentesis, it was recommended by all paired subjects undergoing a paracentesis and all personnel assisting in the procedure.

Clinical efficacy of titanium prepared platelet rich fibrin in periodontal regeneration: A systematic review and meta-analysis

Background: Periodontal regeneration therapies frequently involve autologous platelet concentrates (APCs). They can be used in sinus lift surgeries and socket preservation, among other clinical settings. Platelet rich fibrin (PRF) membrane has been used to treat gingival recession in individuals or groups of individuals using a coronally progressed or lateral pedicle flap. In the treatment of mixed periodontic endodontic lesion/furcation defect, PRF functions as a healing and interpositional biomaterial, filling a cystic cavity. PRF is known to help the bone regeneration process. In the last few years, efforts have been made to enhance the PRFs characteristics and quality. One of them is titanium platelet rich fibrin (T-PRF). Third-generation platelet concentrate no longer contains silica, and its preparation in glass vacuum containers, no longer creates any known concerns. The effectiveness PRF's has been evaluated in connective tissue and bone repair. The aim of this study is to compare T-PRF to other platelet concentrates and different treatment modalities for periodontal regenerative procedures. Methods: A protocol of this systematic review have been registered in prospero (CRD42022293545). The online database searched were PUBMED, COCHRANE for published articles up to November 2022 without language restrictions. Studies in trial registers, handsearching, bibliographic references of relevant articles were also checked. Data collection and analysis was done by individual authors. Independent eligibility assessments were conducted by four review authors. Then, using the standard Cochrane methodology, four review authors extracted the data and evaluated the risk of bias for individual studies. We developed "Summary of findings" tables and used GRADE to evaluate the evidence. Results: Three studies were included for meta-analysis. Results of meta-analysis supported that T-PRF is effective for correction of both hard and soft tissue defects. Conclusions: The overall qualitative and quantitative analysis suggest that T-PRF has superior structural properties and thicker fibrin network for periodontal regenerative procedures.

Abstract No. 58 Comparative Study between a Peristaltic Pump and Vacuum Containers for Paracentesis Management: Time, Flow and Safety

Abstract No. 58 Comparative Study between a Peristaltic Pump and Vacuum Containers for Paracentesis Management: Time, Flow and Safety

A simple preparation protocol for shipping and storage of tissue sections for laser ablation-inductively coupled plasma-mass spectrometry imaging.

A rapid and cost-efficient tissue preparation protocol for laser ablation-inductively coupled plasma-mass spectrometry imaging (LA–ICP–MSI) has been developed within this study as an alternative to the current gold standard using fresh-frozen samples or other preparation techniques such as formalin fixation (FFix) and formalin-fixed paraffin-embedding (FFPE). Samples were vacuum dried at room temperature (RT) and stored in sealed vacuum containers for storage and shipping between collaborating parties. We compared our new protocol to established methods using prostate tissue sections investigating typical endogenous elements such as zinc, iron, and phosphorous with LA–ICP–MSI. The new protocol yielded comparable imaging results as fresh-frozen sections. FFPE sections were also tested due to the wide use and availability of FFPE tissue. However, the FFPE protocol and the FFix alone led to massive washout of the target elements on the sections tested in this work. Therefore, our new protocol presents an easy and rapid alternative for tissue preservation with comparable results to fresh-frozen sections for LA–ICP–MSI. It overcomes washout risks of commonly used tissue fixation techniques and does not require expensive and potentially unstable and time-critical shipping of frozen material on dry ice. Additionally, this protocol is likely applicable for several bioimaging approaches, as the dry condition may act comparable to other dehydrating fixatives, such as acetone or methanol, preventing degradation while avoiding washout effects.

The impact of technological methods of heat treatment of membranes on the properties of the aneroid sensors

The study of the effect of hardering temperature on the properties of beryllium bronze in subsequent aging were made. It is shown that the elastic properties of the aged material, with the hardening is carried out from 750 °C, significantly lower than hardering from 760 °C. With the increase of quenching temperature, this difference decreases after quenching from temperatures 770–800 °С with the level of hardening of the alloy during subsequent aging is practically the same. Comparative studies of the structure and properties of elements heat – treated under different conditions (furnaces with controlled air atmosphere, vacuum containers, molten salts). It is established that the main reason for the shrinkage value is the difference in their structural state caused by the unequal heating time of the material to the aging temperature.

Modified technique for cell-block preparation in effusions

Preoperative and pre-therapeutic morphological diagnosis in the treatment of malignant conditions is the gold standard in the 21st century. There are two standardized methodologies for its implementation - cytological and histological, both of which have their advantages and disadvantages. An intermediate methodology for performing morphological diagnosis in patients with effusions in body cavities and unclear primary locus is the implementation of a cellular block - filtration and centrifugation of large volumes of effusions to optimize the amount of cells, their aggregation, as a result of which the material, taken atraumatically from the patient can be processed similarly to a histological biopsy. The aim of this report is to present a simplified methodology for the realization of a cellular block by means of equipment widely used in the laboratories in Bulgaria, allowing a rapid and efficient implementation suitable for immunohistochemical diagnostics. A standardized laboratory centrifuge, heparinized vacuum containers and 10% buffered formalin were used. Samples from patients with three different tumor localizations were used, the volume of the test effluent being between 5 and 50 mL, centrifuged twice at 5300 rotations per minute and fixed in 10% buffered formalin. The described methodology provides a quick and easy way to morphologically diagnose patients with malignant effusions. The volume of the precipitated material, even with a small amount of biological fluid, is adequate to produce enough slides for diagnosis. The methodology combines the advantages of standard cytology and histology. Demonstrative for the efficiency of the methodology is the identical picture of immunocytochemistry and immunohistochemistry in the case described, where despite the high centrifugation rates both the characteristics of the cells have been retained and the diagnostic proteins have not undergone denaturation.

Non-symmetric bifurcation and collapse of elastic-plastic thick-walled cylinders under combined radial and axial loading

Thick-walled cylinders, which are loaded by accidentally excessive internal or external pressure, axial force or both, may fail due to non-symmetric bifurcation modes with multiple axial and peripheral waves. These failure modes are most relevant to submarines, onshore- and offshore pipelines, vacuum containers, subsea structures, and pressure vessels. Continuum theory is adopted to analyze the bifurcation behavior of such cylinders, considering large elastic-plastic deformation with non-linear hardening. The presented general comprehensive solution is capable of calculating the loads and deformation at the onset of bifurcation for modes of any order in both axial and peripheral directions. As a case study, several bifurcation limit curves of different orders have been calculated using the proposed theoretical solution for a closed thick-walled long cylinder loaded radially and axially. The results are validated numerically by FEA simulations using commercial software. Limit curves of column buckling (first order) and section-collapse (second or higher order) modes of bifurcation are calculated using the proposed solution. The results demonstrate that adding tensile or compressive axial force to external pressure loading delays the occurrence of the section-collapse bifurcation mode.On the other hand, adding external or internal pressure to an axial compression force, the axial strain at column buckling is also increased. The results also explain and quantify the transition between section-collapse and column-buckling bifurcation modes. While the presented results focus on different modes of non-symmetric buckling, the theory is also capable of calculating the axisymmetric and non-symmetric bulging as well as axial necking bifurcation modes. The presented solution offers deeper insight and substantial help to the designers of thick-walled cylindrical components of structures, particularly those subject to excessive external pressure, possibly combined with an axial force.

Concepts for a low-vibration and cryogen-free tabletop dilution refrigerator

The purpose of this article is to describe several concepts of how to cool a modern tabletop dilution refrigerator (DR) with a cryogen-free pulse tube cryocooler (PTC). Tabletop DRs have come more and more into the focus of scientists, recently, because they offer easy access to the mixing chamber mounting plate from all directions and because of their very short cooldown times. However, these milli-Kelvin coolers are precooled with LHe which makes their handling inconvenient and often expensive. In the paper it is explained how a cryocooler can be directly coupled to a DR unit making the use of LHe superfluous. Furthermore, concepts are discussed where a tabletop DR is cooled by a remote PTC; PTC and DR are mounted in separate vacuum containers which are connected by a stainless steel bellows tube. This kind of apparatus would offer an extremely low level of vibration at the mixing chamber mounting plate.

Thermodynamic Analysis of the Reduction of WO&lt;sub&gt;3&lt;/sub&gt; by Al Powder

Hydrogen is widely used to prepare tungsten from tungsten oxides. Such a gas is combustible and strict measures must be taken. However, assisted by Mg or Li3N, tungsten can be prepared through the ball milling at room temperature. The reduction of WO3by Al powder is investigated thermodynamically. Simulations on the loss of Gibbs energy of these hypothetic reactions reveal that the reduction of WO3and WO2(OH)2(or H2WO4) is feasible when Al powder is used; and it is more difficult to generate the intermediate tungsten oxides such as WO2, WO2.72and WO2.9. In addition, it is better to prepare tungsten powder in vacuum containers in view of economy.

Changes in the physical properties of bread during storage

The aim of this work was to compare the physical properties of breadcrumb during five days of storage in vacuum containers and polyethylene bags. On the basis of result it was stated, that storage of baguettes in vacuum condition and in polyethylene foil did not prevent the staling of breadcrumb. Hardness of breadcrumb stored in plastic bags on the fifth day was higher than hardness of bread stored in vacuum containers. The others texture values did not differ significantly on the fifth day of storage between packaging methods. The changes in water activity values both in vacuum containers and polyethylene bags were negligible during storage. Increase in lightness and decrease in yellowness were observed over the storage period, regardless of packaging method, while the values of a* remained essentially unchanged.

Acanthamoebaspp. in Urine of Critically Ill Patients

<i>Acanthamoeba</i>spp. in Urine of Critically Ill Patients

GC-MS Analysis of Contaminants in Breathing Oxygen

Oxygen used for flight crews and for extra-hospital therapies is kept in vacuum containers and maintained as liquid phase (LOX) until use; by using this procedure, the weight of the breathing apparatus is reduced, thus extending breathing oxygen availability and improving safety by using atmosphere pressure sampler. On the other hand, because of its low boiling point (−183°C), during storage and distribution, LOX shows continuous evaporation with a constant increase in pollutant concentration obtained in the LOX production. Therefore, LOX is subjected to a strict quality control to evaluate the contamination levels from production until its use. In this study, an analytical procedure has been optimised to measure the main LOX contaminants (freon, chlorinated solvents, light hydrocarbons, CO2, CO, and N2O) based on a single technique (GC-MS) and two chromatographic columns (Poraplot Q/HT and HP-Plot molecular sieve 5 A). The proposed method is an improvement in terms of time as well cost of analysis and selectivity with respect to the present methods based on three chromatographic techniques (GC-ECD, GC-FID, GC-HID) and four different columns.

Low Temperature Synthesis of Nanocrystalline Silicon and Silicon Oxide Films by Plasma Chemical Vapor Deposition

ABSTRACTNanocrystalline silicon (nc-Si) and SiO2 films have been synthesized at a low substrate temperature using inductively coupled plasma chemical vapor deposition (ICP-CVD) methods with internal low-inductance antenna (LIA) units. The synthesis of these materials was performed in the separate vacuum containers where LIA units were installed so that the induced electric field from an antenna could be used effectively. Radio frequency (13.56 MHz) power was supplied through the matching circuit units. H2 and SiH4 gases were used for nc-Si synthesis, and O2 and SiH4 gases were used for SiO2 deposition. The gas flow ratios were 15 for H2/SiH4 and 4.0 for O2/SiH4. A substrate temperature, gas pressure, RF power and process time were varied in order to investigate optimum conditions for nc-Si synthesis. Silicon oxide films were deposited under conditions of 300°C, 0.2 Pa and 24 mW/cm3. A sample was prepared by SiO2 deposition and subsequent nc-Si synthesis after removing the natural oxide on silicon substrate by buffered 1%-HF (BHF) solution. In some cases, plasma treatments were inserted before or after nc-Si synthesis. The diameter and number density of nc-Si were determined by a high-resolution transmission electron microscopy (HR-TEM). Plan-view TEM images of nc-Si showed that spatially isolated nc-Si was synthesized and that the diameter and the standard deviation of nc-Si could be controlled not only with a substrate temperature, gas pressure, RF power and process time but also with pre/post plasma treatments. The resultant trend suggests that radical precursors and reactive nucleation sites on the SiO2 surface have an important role in the synthesis of nc-Si. The diameter of almost all nc-Si under the present conditions was less than 10 nm. In particular, under conditions of the substrate temperature of 200°C and 4.0 Pa with oxygen plasma pretreatment and hydrogen plasma posttreatment, the mean diameter and number density of nc-Si were 2.7 ± 0.5 nm and 6.5 × 1011 cm−2, respectively. This result is suitable for quantum effect device applications. In addition, electronic properties of a single SiO2 film were examined with the fabricated metal oxide semiconductor (MOS) capacitor. Breakdown voltage was 7.5 MV/cm at 1.0 × 10−6 A/cm2 and leakage current was 1.0 × 10−9 A/cm2 at 2.0 MV/cm for a SiO2 film with a thickness of 12 nm. This result clearly supports the present SiO2 film is capable of the thin dielectric layer of nc-Si devices.

526 大気圧下で回転する超音速フライホイール

The Drag Coefficient of a rotating article in the fluid is reduced drastically by effect of the newly developed an aerodynamic system that has Floating Shrouds, up to Supersonic Velocity. Details of the system are disclosed as a theoretical study on the fluid dynamics and experimental observations are described in this paper. We produced some rotors for experiment as flywheels they rotate extremely great angular velocity and high tangential velocity under the atmospheric pressure, and vacuum containers to reduce the friction loss of rotors rotating on high speed are in no use. And we make analogical reasoning on electric circuits.

Evaluation of vacuum containers for consumer storage of fruits and vegetables

Plastic containers, capable of being evacuated to 50 kPa, were investigated for maintaining quality of fruits and vegetables. Celery, lettuce, broccoli, grapes, green beans, melon, and strawberry were kept at 4°C, broccoli, okra and tomato were kept at 8°C in vacuum containers and in conventional, sealed, plastic food containers. For each produce item, similar concentrations of carbon dioxide accumulated in each container type. After sealing containers for 3 days oxygen fell below 5 kPa for strawberry, okra and broccoli in conventional containers and for all types of produce in vacuum containers. The color of produce in vacuum containers was usually darker (lower L value) and less saturated (lower chroma) than in conventional containers. A higher proportion of strawberry fruits were infected by fungi in vacuum containers than in conventional. Water soaked lesions appeared on green beans kept in vacuum containers. Okra in vacuum showed less darkening of seeds than in conventional containers. Overall the vacuum containers showed little advantage over conventional containers for the types of produce tested. © 2000 Elsevier Science B.V. All rights reserved.

Stability of amphotericin B in an extemporaneously prepared i.v. fat emulsion.

The stability of amphotericin B in an extemporaneously prepared i.v. fat emulsion was studied. Admixtures of amphotericin B 0.5, 1, and 2 mg/mL were prepared by adding 10, 20, and 40 mL of amphotericin B 5 mg/mL to 90, 80, and 60 mL, respectively, of 20% fat emulsion. The admixtures were stored in glass vacuum containers at 20-25 degrees C and exposed to fluorescent light, 20-25 degrees C and protected from light, or 4-8 degrees C and protected from light. A sample was withdrawn from each container at 0, 4, 12, and 24 hours and at 2, 4, 7, and 15 days for analysis of amphotericin B concentration by high-performance liquid chromatography and for visual evaluations; these samples were immediately frozen until analyzed. A sample was withdrawn from one container of amphotericin B 1 and 2 mg/mL for each storage condition at 0, 7, and 15 days for immediate determination of particle-size distribution with a fluorescinated-antibody cell sorter. Amphotericin B 0.5 mg/mL in 20% fat emulsion was stable for one week under all the storage conditions. Amphotericin B in the 1- and 2-mg/mL admixtures was stable for up to four days at 20-25 degrees C exposed to fluorescent light, and for up to one week at 20-25 degrees C protected from light and at 4-8 degrees C protected from light. There was no visible evidence of incompatibility. There were no substantial changes in particle-size distribution for the 1-mg/mL admixtures; appreciable changes were detected for the 2-mg/mL admixtures. Amphotericin B 1 and 2 mg/mL was stable in 20% fat emulsion for four days at 20-25 degrees C exposed to fluorescent light and for seven days at 20-25 degrees C protected from light or at 4-8 degrees C; amphotericin B 0.5 mg/mL was stable in 20% fat emulsion for seven days under the three storage conditions.

Pyroelectric PVF_2 infrared detector arrays

Linear arrays of thin membrane (0.5-0.8-microm) pyroelectric PVF(2) detectors have been constructed. The size of each element was 0.1 x 3 mm. The arrays were built with twenty-four channels of hybrid self-scanning electronics and packaged in vacuum containers, D* of 1.5 x 10(9) cm/Hz/W at 10 Hz and thermal cross talk of <15% at 50 Hz were obtained.

Deep freezed sperm (author's transl)

Since the invention of vacuum containers for liquid nitrogen to keep up a constant temperature of ––196.5 °C, and the use of glycerin as cell-protecting substance, it was possible to store human semen at low temperatures for inseminations. Our intention was to keep the damage rate of spermatozoa during the freezing and thawing process as low as possible. Therefore, we tested five different methods. We used different liquids as sperm protectors and tried different procedures of freezing and thawing. The best way for our equipment was the combination of paillettes as sperm containers, a continuous freezing process, thawing at 20 °C, and Matheson’s liquid as sperm-protective substance. Biological and ultrastructural tests of human semen after freezing are improving.