Vaccinium Angustifolium Research Articles

Have native insect pests associated with a native crop in Maine declined over the past three to five decades?

Abstract Lowbush blueberry is an important berry crop in Maine (USA), Quebec and the Maritime provinces of Canada. The other economically important crops in Maine are potato, dairy and organic mixed vegetable produce. Lowbush blueberry is a complex of 1–4 Vaccinium species, primarily dominated by Vaccinium angustifolium Aiton. All but one of the insect pest species in this crop system are native to the regions where it is managed in North America. Insect decline has been shown to be a recent global phenomenon although it has rarely been studied in agricultural ecosystems. Long‐term datasets of insect species abundances are a powerful tool for insect population trends. To determine if native insect pests have declined over the past several decades in Maine lowbush blueberry, we surveyed three native species. All surveys were conducted in commercial blueberry fields; although, none of the sample sites were sprayed with insecticides. Insect pest sampling comprised two surveys (1961–2014 and 1998–2017) conducted on the larval stage of the blueberry maggot fly (Rhagoletis mendax Curran), one survey (1981–2016) on the larval stage of the blueberry spanworm (Itame argillacearia (Packard)), and one survey (1983–2019) on the larval stage of the blueberry flea beetle (Altica sylvia Malloch). Based upon the analysis of our long‐term population‐level datasets, we found no evidence that insect decline has occurred in this native blueberry insect pest complex intimately associated with the lowbush blueberry, a native crop plant in North America, despite recent documentation of rapid climate change occurring in the lowbush blueberry production areas.

Pesticide Contamination in Native North American Crops, Part II-Comparison of Flower, Honey Bee Workers, and Native Bee Residues in Lowbush Blueberry.

In lowbush blueberry fields, we conducted residue analysis comparing flowers, trapped pollen (honey bee and Osmia spp.), and collected bees (honey bee workers, bumble bee queens, and non-Bombus spp. wild native bees). The study was conducted from 2012 to 2014. The number of pesticide residues, total concentrations, and risk to honey bees (Risk Quotient) on flowers were not significantly different from those determined for trapped honey bee pollen (except in one study year when residues detected in flower samples were significantly lower than residue numbers detected in trapped pollen). The compositions of residues were similar on flowers and trapped pollen. The number of residues detected in honey bee pollen was significantly greater than the number detected in Osmia spp. pollen, while the total concentration of residue was not different between the two types of pollen. The risk to honey bees was higher in trapped honey bee pollen than in trapped Osmia spp. pollen. The analysis of honey bee workers, native bumble bee queens, and native solitary bees showed that although more pesticide residues were detected on honey bee workers, there were no differences among the bee taxa in total residue concentrations or risk (as estimated in terms of risk to honey bees).

Prospects for Enhanced Growth and Yield of Blueberry (Vaccinium angustifolium Ait.) Using Organomineral Fertilizers for Reclamation of Disturbed Forest Lands in European Part of Russia

This article presents the results of research on the use of a new granular organomineral fertilizer for growing plants of half-highbush cultivars of the lowbush blueberry (Vaccinium angustifolium Ait.). The purpose of the research is studying the effect of a new granular organomineral fertilizer on the agrobiological characteristics of V. angustifolium plants on a peatland in the conditions of the north of the European part of Russia. The developed composition of organomineral fertilizer (NPK 8:8:8, Fe 0.5%, Zn 0.2%, Cu 0.4%) differs from existing fertilizers in a more balanced ratio of micro- and macroelements and the content of vermicompost containing spore forms of bacteria Bacillus subtilis H-13, B. mucilaginosus, and Azotobacter chroococcum. The use of a new organomineral fertilizer on high-moor peat (pH 2.9) contributed to the highest yield of V. angustifolium fruits (190.2 g/bush), which is 1.2 times more than other mineral fertilizers (NPK, etc.). The best morphophysiological indicators of V. angustifolium plants (leaf area, total and working surface of the roots, root weight) when using the developed organomineral fertilizer were noted in September. An increase of 1.2–1.3 times in the collection of dry matter and sugars in fruits was revealed, compared with other fertilizer options, while the vitamin C content practically did not change. New organomineral fertilizer contributes to the optimal supply of the necessary micro- and macroelements throughout the growing season and increases the productivity of V. angustifolium plants when growing depleted peat deposits in the natural and climatic conditions of the European part of Russia.

Pesticide Contamination in Native North American Crops, Part I-Development of a Baseline and Comparison of Honey Bee Exposure to Residues in Lowbush Blueberry and Cranberry.

A pesticide exposure baseline for honey bees was compiled for two New England cropping systems, the native North American plant species consisting of lowbush blueberry (Vaccinium angustifolium Aiton) and cranberry (Vaccinium macrocarpon Aiton). More unique pesticide compounds were applied in blueberry than cranberry, but the numbers of pesticides discovered in trapped honey bee pollen were similar between the two crop systems. Not all pesticides found in pollen were the result of the applications reported by growers of either crop. When comparing residues, number of pesticides detected, total concentration, and risk quotient varied between the two crops. Also, blueberry was dominated by fungicides and miticides (varroacides) and cranberry was dominated by insecticides and herbicides. When comparing reported grower applications that were matched with detection in residues, the proportion of pesticide numbers, concentrations, and risk quotients varied by crop system and pesticide class. In most cases, pesticide residue concentrations were of low risk (low risk quotient) to honey bees in these crops. Estimation of decay rates of some of the most common pesticide residues under field conditions could aid growers in selection of less persistent compounds, together with safe application dates, prior to bringing in honey bees for pollination.

Genetic and epigenetic signatures for improved breeding of cultivated blueberry.

Blueberry belongs to the Vaccinium genus and is a highly popular fruit crop with significant economic importance. It was not until the early twentieth century that they began to be domesticated through extensive interspecific hybridization. Here, we collected 220 Vaccinium accessions from various geographical locations, including 154 from the United States, 14 from China, eight from Australia, and 29 from Europe and other countries, comprising 164Vaccinium corymbosum, 15Vaccinium ashei, 10 lowbush blueberries, seven half-high blueberries, and others. We present the whole-genome variation map of 220 accessions and reconstructed the hundred-year molecular history of interspecific hybridization of blueberry. We focused on the two major blueberry subgroups, the northern highbush blueberry (NHB) and southern highbush blueberry (SHB) and identified candidate genes that contribute to their distinct traits in climate adaptability and fruit quality. Our analysis unveiled the role of gene introgression from Vaccinium darrowii and V. ashei into SHB in driving the differentiation between SHB and NHB, potentially facilitating SHB's adaptation to subtropical environments. Assisted by genome-wide association studies, our analysis suggested VcTBL44 as a pivotal gene regulator governing fruit firmness in SHB. Additionally, we conducted whole-genome bisulfite sequencing on nine NHB and 12 SHB cultivars, and characterized regions that are differentially methylated between the two subgroups. In particular, we discovered that the β-alanine metabolic pathway genes were enriched for DNA methylation changes. Our study provides high-quality genetic and epigenetic variation maps for blueberry, which offer valuable insights and resources for future blueberry breeding.

An Economic Cost/Benefit Tool to Assess Bee Pollinator Conservation, Pollination Strategies, and Sustainable Policies: A Lowbush Blueberry Case Study

Lowbush blueberry is a mass-flowering plant species complex that grows in both unmanaged wild landscapes and managed agricultural fields in northeastern regions of both the USA and Canada. During pollination, more than 120 native bee species are associated with lowbush blueberry ecosystems in Maine, USA, in addition to three commercially managed bees. Over a 29-year period, we sampled 209 lowbush blueberry fields using quadrat and transect sampling, recording both native bee and honey bee densities, honey bee hive stocking density, and native bees as a proportion of total bees. These data were used to simulate economic uncertainty in pollination. We developed a novel algorithm, the Economic Pollinator Level (EPL), to estimate bee densities that economically warrant pollination investments such as rented hives and planting bee pastures. Statistical modeling indicated both native bee and honey bee activity density predicted proportion fruit set in fields. Honey bee activity density was well predicted by hive stocking density. Proportion fruit set adequately predicted yield. EPL was most sensitive to fruit set/m2/bee and less dependent on berry weight, rented hive stocking density, hive rental cost, lowbush blueberry price, and the annual cost of planting/maintaining pollinator pastures. EPL can be used to sustainably balance economical pollination investments/decisions with bee conservation in lowbush blueberry crops and potentially other pollinator-dependent crops.

How will climate change and forest harvesting influence the habitat quality of two culturally salient species?

Boreal landscapes face increasing disturbances which can affect cultural keystone species, i.e. culturally salient species that shape in a major way the cultural identity of a people. Given their importance, the fate of such species should be assessed to be able to act to ensure their perennity. We assessed how climate change and forest harvesting will affect the habitat quality of Rhododendron groenlandicum and Vaccinium angustifolium, two cultural keystone species for many Indigenous peoples in eastern Canada. We used the forest landscape model LANDIS-II in combination with species distribution models to simulate the habitat quality of these two species on the territories of three Indigenous communities according to different climate change and forest harvesting scenarios. Climate-sensitive parameters included wildfire regimes as well as tree growth. Moderate climate change scenarios were associated with an increased proportion of R. groenlandicum and V. angustifolium in the landscape, the latter species also responding positively to severe climate change scenarios. Harvesting had a minimal effect, but slightly decreased the probability of presence of both species where it occurred. According to the modeling results, neither species is at risk under moderate climate change scenarios. However, under severe climate change, R. groenlandicum could decline as the proportion of deciduous trees would increase in the landscape. Climate change mitigation strategies, such as prescribed fires, may be necessary to limit this increase. This would prevent the decrease of R. groenlandicum, as well as contribute to preserve biodiversity and harvestable volumes.

A deep multi-task learning approach to identifying mummy berry infection sites, the disease stage, and severity.

Mummy berry is a serious disease that may result in up to 70 percent of yield loss for lowbush blueberries. Practical mummy berry disease detection, stage classification and severity estimation remain great challenges for computer vision-based approaches because images taken in lowbush blueberry fields are usually a mixture of different plant parts (leaves, bud, flowers and fruits) with a very complex background. Specifically, typical problems hindering this effort included data scarcity due to high manual labelling cost, tiny and low contrast disease features interfered and occluded by healthy plant parts, and over-complicated deep neural networks which made deployment of a predictive system difficult. Using real and raw blueberry field images, this research proposed a deep multi-task learning (MTL) approach to simultaneously accomplish three disease detection tasks: identification of infection sites, classification of disease stage, and severity estimation. By further incorporating novel superimposed attention mechanism modules and grouped convolutions to the deep neural network, enabled disease feature extraction from both channel and spatial perspectives, achieving better detection performance in open and complex environments, while having lower computational cost and faster convergence rate. Experimental results demonstrated that our approach achieved higher detection efficiency compared with the state-of-the-art deep learning models in terms of detection accuracy, while having three main advantages: 1) field images mixed with various types of lowbush blueberry plant organs under a complex background can be used for disease detection; 2) parameter sharing among different tasks greatly reduced the size of training samples and saved 60% training time than when the three tasks (data preparation, model development and exploration) were trained separately; and 3) only one-sixth of the network parameter size (23.98M vs. 138.36M) and one-fifteenth of the computational cost (1.13G vs. 15.48G FLOPs) were used when compared with the most popular Convolutional Neural Network VGG16. These features make our solution very promising for future mobile deployment such as a drone carried task unit for real-time field surveillance. As an automatic approach to fast disease diagnosis, it can be a useful technical tool to provide growers real time disease information that can prevent further disease transmission and more severe effects on yield due to fruit mummification.

Sea Cucumber and Blueberry Extracts Suppress Inflammation and Reduce Acute Lung Injury through the Regulation of NF-κB/MAPK/JNK Signaling Pathway in Lipopolysaccharide-Treated C57BL/6 Mice.

Acute lung injury (ALI) represents a life-threatening condition with high morbidity and mortality despite modern mechanical ventilators and multiple pharmacological strategies. Therefore, there is a need to develop efficacious interventions with minimal side effects. The anti-inflammatory activities of sea cucumber (Cucumaria frondosa) and wild blueberry (Vaccinium angustifolium) extracts have been reported recently. However, their anti-inflammatory activities and the mechanism of action against ALI are not fully elucidated. Thus, the present study aims to understand the mechanism of the anti-inflammatory activity of sea cucumber and wild blueberry extracts in the context of ALI. Experimental ALI was induced via intranasal lipopolysaccharide (LPS) instillation in C57BL/6 mice and the anti-inflammatory properties were determined by cytokine analysis, histological examination, western blot, and qRT-PCR. The results showed that oral supplementation of sea cucumber extracts repressed nuclear factor kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways, thereby downregulating the expression of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) in the lung tissue and in the plasma. Wild blueberry extracts also suppressed the expression of IL-4. Furthermore, the combination of sea cucumber and wild blueberry extracts restrained MAPK signaling pathways by prominent attenuation of phosphorylation of NF-κB, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK) while the levels of pro-inflammatory cytokines were significantly suppressed. Moreover, there was a significant and synergistic reduction in varying degrees of ALI lesions such as distorted parenchyma, increased alveoli thickness, lymphocyte and neutrophil infiltrations, fibrin deposition, pulmonary emphysema, pneumonia, intra-alveolar hemorrhage, and edema. The anti-inflammatory effect of the combination of sea cucumber and wild blueberry extracts is associated with suppressing MAPK and NF-κB signaling pathways, thereby significantly reducing cytokine storm in LPS-induced experimental ALI.

Population Structure and Reproductive Biology of Monilinia vaccinii-corymbosi in Vaccinium angustifolium in Maine.

The fungus Monilinia vaccinii-corymbosi (Mvc) causes mummy berry disease in blueberries including lowbush blueberry, Vaccinium angustifolium, and is a significant pathogen of concern for Maine lowbush blueberry growers. This disease is typically managed with fungicides or by burning of plant debris containing overwintering pseudosclerotia. The population structure of Mvc in various fields in Maine was investigated using microsatellites and isolates collected from three stages in the Mvc lifecycle. The impacts of management strategies were also examined. A high level of genetic diversity was observed in Mvc from 12 lowbush blueberry fields with 199 unique multilocus haplotypes (MLHs) occurring in an original sample of 232 isolates. Twelve private alleles, including six private alleles with frequencies above 0.05, which indicated gene flow, were observed in six out of 12 fields. The population of Mvc in Maine as a whole is mostly a sexual, outcrossing population, as was seen in the diversity of MLHs and low amounts of linkage disequilibrium, although some apothecia appear to result from selfing. Three fields appear to have some clonal reproduction but were not strictly clonal, as multiple MLHs were noted in these fields. Management does not appear to affect population structure, and Mvc may be one large statewide population in Maine.

Underutilized Canadian wild berries as potential sources of lipophilic bioactive compounds with antihypertensive properties.

Traditional berries are small fruits and are widely distributed in the Canadian prairies. The current study investigates the lipophilic bioactive compounds such as fatty acids, phytosterols, and terpenes, and their bioactivities, such as lipid peroxidation, as well as the antihypertensive activities of fourteen underutilized Canadian wild berries. These berries include Saskatoon berries (Amelanchier alnifolia), gooseberries (Ribes hirtellum), wild grapes (Vitis riparia), blackcurrants (Ribes nigrum), redcurrants (Ribes rubrum), haskap berries (Lonicera caerulea), wild raspberries (Rubus idaeus), wild blueberries (Vaccinium angustifolium), chokeberries (Aronia melanocarpa), buckthorn (Rhamnus cathartica), highbush cranberries (Viburnum trilobum), chokecherries (Prunus virginiana), nannyberries (Viburnum lentago) and snowberries (Symphoricarpos albus). The fatty acids, phytosterols, and terpenes were identified using Gas Chromatography-Mass Spectrometry (GC-MS). Lipid peroxidation and the antihypertensive activity assessed by measuring the berries' angiotensin converting enzyme 1 (ACE1) inhibitory activity were determined using in vitro methods. Notably, wild grapes exhibited the highest (p < 0.05) total fat content (7659 ± 312 μg per g DW), followed by haskap berries (4650 ± 184 μg per g DW). Polyunsaturated fatty acids (PUFAs) were highest (p < 0.05) in wild grapes (74%). Predominant phytosterols and terpenes identified in Canadian wild berries included β-sitosterol, isofucosterol, phytol, and α-amyrin. Saskatoon berries and gooseberries showed a distinct phytosterol and terpene profile compared to the other wild berries. Snowberries demonstrated the highest (p < 0.05) lipid peroxidation and the lowest (p < 0.05) angiotensin converting enzyme (ACE1) activity. This research provides valuable insights into the lipophilic bioactive compounds and their potential activities in vitro of the Canadian wild berries, offering a foundation for further exploration and potential applications in the context of nutraceuticals and functional foods.

Evaluation of sequential mesotrione application rates and sequential tolpyralate and mesotrione applications for narrow-leaved goldenrod management in lowbush blueberry

Sequential mesotrione applications of 72 g a.i. ha−1 gave similar control of narrow-leaved goldenrod as sequential applications of 144 g a.i. ha−1. This use pattern is recommended for narrow-leaved goldenrod management in lowbush blueberry. Early POST tolpyralate applications exhibited good crop tolerance and could be considered as part of a sequential herbicide program for lowbush blueberry.

Extending the postharvest life of wild blueberries with proprietary plastic pallet covers

Wild blueberries (Vaccinium angustifolium Ait) face limited consumption due to their short production period of one month. The lack of specialized storage infrastructure quickly saturates the local market. A cost-effective solution using existing facilities and proprietary plastic pallet covers is proposed. This alternative aims to extend the shelf life of wild blueberries to remain marketable for extended periods. This project aimed to evaluate several parameters to optimize storage conditions specifically for this fruit from the Lac-Sainte-Jean region of Quebec. Two seasons, 2021 and 2022, were considered, and the experimental setup included two temperature regimes (0 and 4 °C), four different polymer types (polyethylene; Lifespan®, and PrimePro®, SPAC®), three carbon dioxide concentrations (0 %, 10 %, and 20 %), the presence or absence of sulfur dioxide (SO2) releasing patches, and two fruit types (sorted or unsorted). Fruit quality was then evaluated, considering weight loss on the pallet, berry color, firmness, acidity, total soluble solids, and overall quality. In both cases, the fruit was stored for approximately one month. It was observed that fruit could be kept effectively for up to one month using PrimePro® covers at 0 °C.

Characterization and modification of biochar from a combined heat and power (CHP) plant for amending sandy soils collected from wild blueberry fields

Wild (or lowbush) blueberries (Vaccinium angustifolium Ait.) undergo severe drought impacts due to climate warming because they grow in sandy soils with poor water retention. The feasibility was studied for using biochar in a forest biomass-fueled combined heat and power (CHP) plant to amend the sandy soils. The chemico-physical properties (e.g., bulk density, moisture content, porosity, pH) of the biochar were measured. An acid treatment method (1% to 3% acidic or citric acid solution) was developed to decrease the biochar pH from 11.4 to neutral or lower, aiming to aid in weed control in wild blueberry fields. The water holding capacity (WHC) of sandy soils (S) mixed with biochar (B) (Type I) and sandy soils mixed with both biochar and fertilizer (Type II) at four ratios of 100S:0B (control), 50S:50B, 30S:70B, and 10S:90B were measured. The biochar generated from the CHP plant had comparable physical properties (such as bulk density, porosity, pH, and surface area) with woody biochar made from pyrolysis. The acid treatment method significantly lowered the pH to a range of 5.0 to 6.5. The 50:50 mixing ratio for both Type I and Type II increased the water holding capacity by about 20% compared with control groups.

Effect of Mechanical Harvesting Technology Type and Harvester Ownership and Services Acquisition Methods on Profitability of Wild Blueberry Production

ABSTRACT The profitability of wild blueberry (Vaccinium angustifolium) production using two alternative mechanical harvesters was evaluated under three different harvester ownership/service arrangements commonly used by farmers. Production data for the economic analysis were obtained from on-farm trials conducted in Nova Scotia, Canada. Net returns were CAD$323 ha−1 using a semi-automatic bin handling system compared with CAD$281 ha−1 for a small box handling system with outright harvester purchase. By comparison, net returns were CAD$90 ha−1 using the semi-automatic bin handling system and CAD$63 ha−1 using the small box system using rental harvesting services. The results are more sensitive to changes in yield than price.

Systemic insecticides for control of stem gall wasp in highbush blueberry.

The gall wasp, Hemadas nubilipennis Ashmead, is a pest of highbush and lowbush blueberry and can pose a challenge to control with foliar sprays due to adult activity being during bloom and because larval development is within plant tissues. We hypothesized that systemic insecticides that move within the blueberry vascular system would reach areas where H. nubilipennis eggs are laid, causing larval mortality. Three application methods, crown injection, soil drench, and foliar spray were applied to potted 'Jersey' blueberry bushes at 50% and 100% rates to quantify systemic residue concentrations in shoots and leaves. Additionally, systemic insecticides were evaluated for control of gall wasps using single-shoot bioassays and measuring larval mortality at 0.01%, 0.1%, 1%, and 10% of field rate provided within a floral pick. Systemic insecticides tested in both studies included imidacloprid, flupyradifurone, and spirotetramat. The potted bush residue study determined that insecticides moved from three tested sites of entry: the roots, crown cavity, and foliage. Results from the shoot bioassays found that the mean percent larval survival of H. nubilipennis was negatively correlated with the concentration of AI detected in galls. Imidacloprid and spirotetramat were found to have the greatest potential for control of H. nubilipennis due to mortality in the shoot bioassays and similar residue concentrations in the potted bush studies to shoot bioassays. Future research should evaluate systemic insecticides applied in highbush blueberry plantings for control of H. nubilipennis using the bioassay mortality assessment method developed in this study.

Antiatherogenic effect of blueberry fruit polyphenols extracts (Vaccinium angustifolium Ait) reduces oxidative stress and enhances the cholesterol efflux process

Antiatherogenic effect of blueberry fruit polyphenols extracts (Vaccinium angustifolium Ait) reduces oxidative stress and enhances the cholesterol efflux process

Intraspecific trait variation as a mechanism of coexistence of congeneric blueberry (Vaccinium) species after forest harvesting

Understorey shrubs of boreal forests grow vigorously after canopy removing disturbance such as clearcutting and fire and dominate the post-disturbance community. Many of these dominant shrubs are morphologically and phylogenetically closely related. How do they coexist despite having similar traits and resource needs? One way the coexisting plants may avoid competition is by niche differentiation that may alleviate the negative effects of competition due to high niche overlap. We studied two blueberry species, Vaccinium angustifolium and V. myrtilloides often found in proximity to one another after forest harvesting to test the hypothesis that coexisting congeneric species differentiate their resource acquisition and use through variation in their functional traits (niche differentiation). Discovering the coexistence mechanisms of Vaccinium spp. that flourish after forest harvesting and fire is important from both theoretical and applied perspectives. Vaccinium spp. are dominant shrubs after forest harvesting and fire and constitute a significant food source for wildlife and health-conscious consumers and are also of cultural significance to indigenous peoples. From three post-harvest forest sites in northwestern Ontario, we measured cover of Vaccinium spp. in 81 1 × 1 m quadrats where they occurred alone and together. We then excavated 103 ramets of the two Vaccinium spp. occurring alone and together after measuring three habitat variables (organic matter depth, soil moisture and soil temperature) in the immediate surroundings of the excavated plants. We measured five functional traits: height, canopy diameter, specific leaf area, specific stem density and specific root length. Using these data, we calculated functional hypervolumes (niche space occupancy) of each species occurring alone and together. We also calculated probabilistic niche overlap between the two species as well as their reaction norms to habitat variables. We found that species hypervolumes changed in size, and the probability and degree of overlap between the two Vaccinium spp. changed when occurring alone and co-occurring. Linear regression showed no significant effect of habitat factors on traits. We detected high intraspecific variation in some traits leading to shifts in hypervolumes when they co-occurred. We conclude that at fine spatial scales (homogeneous habitats, open canopy condition) individual trait variation allows for niche complementarity promoting coexistence among these important, early succession, closely related species.

Microbiome of Ceca from Broiler Chicken Vaccinated or Not against Coccidiosis and Fed Berry Pomaces.

American cranberry (Vaccinium macrocarpon) and lowbush/wild blueberry (V. angustifolium) pomace are polyphenol-rich products having potentially beneficial effects in broiler chickens. This study investigated the cecal microbiome of broiler-vaccinated or non-vaccinated birds against coccidiosis. Birds in each of the two groups (vaccinated or non-vaccinated) were fed a basal non-supplemented diet (NC), a basal diet supplemented with bacitracin (BAC), American cranberry (CP), and lowbush blueberry (BP) pomace alone or in combination (CP + BP). At 21 days of age, cecal DNA samples were extracted and analyzed using both whole-metagenome shotgun sequencing and targeted-resistome sequencing approaches. Ceca from vaccinated birds showed a lower abundance of Lactobacillus and a higher abundance of Escherichia coli than non-vaccinated birds (p < 0.05). The highest and lowest abundance of L. crispatus and E. coli, respectively, were observed in birds fed CP, BP, and CP + BP compared to those from NC or BAC treatments (p < 0.05). Coccidiosis vaccination affected the abundance of virulence genes (VGs) related to adherence, flagella, iron utilization, and secretion system. Toxin-related genes were observed in vaccinated birds (p < 0.05) in general, with less prevalence in birds fed CP, BP, and CP + BP than NC and BAC (p < 0.05). More than 75 antimicrobial resistance genes (ARGs) detected by the shotgun metagenomics sequencing were impacted by vaccination. Ceca from birds fed CP, BP, and CP + BP showed the lowest (p < 0.05) abundances of ARGs related to multi-drug efflux pumps, modifying/hydrolyzing enzyme and target-mediated mutation, when compared to ceca from birds fed BAC. Targeted metagenomics showed that resistome from BP treatment was distant to other groups for antimicrobials, such as aminoglycosides (p < 0.05). Significant differences in the richness were observed between the vaccinated and non-vaccinated groups for aminoglycosides, β-lactams, lincosamides, and trimethoprim resistance genes (p < 0.05). Overall, this study demonstrated that dietary berry pomaces and coccidiosis vaccination significantly impacted cecal microbiota, virulome, resistome, and metabolic pathways in broiler chickens.

“We’re Farmers, Not Beekeepers”: a Cultural Model of Pollination Management Among Lowbush Blueberry Growers in the United States and Canada

In this article, we present a cultural model of lowbush blueberry growers’ pollination management. Through content analysis of semi-structured interviews with growers in Maine, United States, and Prince Edward Island, Canada, we identify a shared, tacit model used by growers to understand crop pollination and management. This cultural model explains growers’ perceptions of and attitudes toward pollination; this informs their management practices. Growers believe that pollination is a crucial component of crop management and design their management strategies with consideration for three distinct sources of pollination—honeybees, other commercially managed bees, and wild bees. On-farm pollinator conservation is a strategy growers use to manage uncertainty rather than a distinct schema in growers’ cultural model. We discuss ways that outreach professionals can consider growers’ cultural models when designing communications and trainings about pollinator conservation. We argue that cultural modeling can improve understanding among groups with shared interests yet different perceptions, such as farmers, researchers, and Cooperative Extension agents.