UV-Vis Spectrophotometry Method Research Articles

Optimizing carry-over in automated dissolution system dissoBOT for paracetamol and diclofenac sodium analysis

In this work, an automated dissolution system (dissoBOT) was used for dissolution testing for the first time. Carry-over (CO) of the dissoBOT was determined for paracetamol (PA) and diclofenac sodium (DS), which are active pharmaceutical ingredients (APIs). Initially, partial method validation of the UV-VIS spectrophotometry method for PA and DS determination was performed by defining the limit of detection (LOD), the limit of quantification (LOQ), accuracy, and precision. The LODs and LOQ were less than 0.01 mg/L for both APIs. The determined linear concentration ranges were from 1.00 mg/L to 30.00 mg/L for PA and from 0.50 mg/L to 3.50 mg/L for DS (the square of the correlation coefficient was greater than 0.9990, and the quality coefficient was less than 1.00% for both APIs). The accuracy of the method was evaluated by calculating the recovery (Re) of the solutions of standards with known concentrations. The method for both APIs was deemed to be accurate (the average Re for PA and DS were 99.81% and 101.43%, respectively). Precision was evaluated by calculating the relative standard deviation (RSD). The method for PA and DS was deemed to be precise, as the RSD value for PA was 0.13%, and for DS was 0.38%. The volume (V) of the washing medium in both cleaning cycles performed by the dissoBOT system, as well as the medium dispensing V, were established, where the medium dispensing V was in accordance with the United States Pharmacopeia requirements. The CO of the dissoBOT system, using tap water as the washing medium, was determined to be less than 1.00% for both APIs. The CO values for one cleaning cycle of the sampling station with a V of 2 mL was in the range of 1.24–1.54%, for V of 5 mL was in the range of 0.78–0.93%, and for V of 10 mL was in the range of 0.27–0.36%. In addition, the CO of the dissoBOT, when employing two cleaning cycles of the sampling station (each V of 10 mL) was reduced (CO <0.20%). Finally, the dissoBOT was successfully employed for the dissolution PA and DS tables.

Development and Validation of UV-visible Spectrophotometry Method for Simultaneous Estimation of Rutin and Curcumin in Bulk Pharmaceuticals

Development and Validation of UV-visible Spectrophotometry Method for Simultaneous Estimation of Rutin and Curcumin in Bulk Pharmaceuticals

Determination Of Total Flavonoids Extract of White (Magnolia Alba (Dc.) Figlar) Using Spectrophotometry UV-Vis Method

Flavonoids are a group of secondary metabolites produced by plants that have the potential to influence pharmacological activity. This study aims to determine the total flavonoid content of the ethanol crude extract of white cempaka flowers (Magnollia alba(DC.) Figlar) using the UV-Vis spectrophotometry method. This study used colorimetric and spectrophotometric methods using quercetin as a comparison. The qualitative test research results proved that cempaka putih (Magnolia alba (DC.) Figlar) flowers contain flavonoid coumpound. Then a maximum wavelength of 432 nm is obtained with an absorbance of 0.1236, the absorbance calculation for each quercetin concentration and the sample is calculated using a maximum wavelength of 432 nm. Based on the linear regression equation obtained from the standard quercetin curve, the total flavonoid content of the crude ethanol extract of cempaka putih flowers with 3 different concentration variations, at a concentration of 100 ppm, the results obtained for the total flavonoid content were 276,83 ± 17,7 mgQE/g, 500 ppm. total flavonoid levels of 105,189 ± 8,16 mgQE/g, 1000 ppm obtained total flavonoid levels of 79,115 ± 8,33 mgQE/g.

CITRUS FRUITS IN HEALTH CARE WITH A SPECIAL REFERENCE TO THE USES OF TRIBAL PEOPLE OF TRIPURA

Aim: Method validation is a step to ensure that the method used is in accordance with the desired goals. The Category I validation method determines the content of the main ingredient in the drug substance. Galactomannan is a watersoluble polysaccharide composed of D-galactose and D-mannose and is the main component in guar gum. Materials and Methods: To determine galactomannan content, concentrated phenol-sulfuric acid was used using the UV-Vis Spectrophotometry method. Hydrolysis of guar gum is required to react with phenolic reagents to form colored compounds. Results and Discussion: Optimization of the formation of colored derivative compounds was carried out by hydrolysis of guar gum with the addition of 1.0 mL of sulfuric acid, 30 min time, 100°C temperature, and color stability at 180 min. Method validation was successfully carried out by testing several parameters, including selectivity with the selected wavelength 492 nm. Linear regression equation y = 0.0255 × + 0.0420 (r: 0.9998) and Vxo = 0.76%. The precision expressed in the Coefficient of Variation (C.V) is 1.06%. Accuracy was obtained 100.35 ± 0.56 (%; w/w). Conclusion: In determining the galactomannan content in guar gum powder, it was 70.00 ± 0.24 (%; w/w).

A Comprehensive Examination of UV-VIS Spectrophotometric Methods in Pharmaceutical Analysis Between 2015-2023.

Quality control is a system of validated procedures in which many samples, including active pharmaceutical ingredients and final products, are analyzed using standard or validated analytical methods. Analytical methods used in analyzing active pharmaceutical ingredients or final products in the pharmaceutical industry can be methods registered in pharmacopeias and developed by the company itself. For this reason, published papers related to pharmaceutical analysis attract analysts and researchers' attention. In this study, pharmaceutical analysis and bioanalysis studies carried out between 2015 and 2023 were examined using Google Scholar, and the recent trends were determined for pharmaceutical analysis. Among the published papers performing conventional analytical techniques for pharmaceutical analysis, those applying UV-VIS spectrophotometry method were selected to predict a future perspective in this study. In addition to the data obtained, the current situation of the pharmaceutical industry was considered to correlate with the obtained data for pharmaceutical analysis. The results were presented with comparative tables and summarizing graphs. Interpreting the results allowed us to determine the trends that pharmaceutical analysis studies will lead in the future. This study can be helpful for researchers working on pharmaceutical analysis in both the industry and academia to predict future trends in pharmaceutical analysis. As a result of the literature research covering the dates 2015-2023, 56% of UV-VIS Spectrophotometric methods are used on pharmaceutical dosage forms, 27% are bulk, 16% are pure, 2% are biological materials, and 0.4% are herbal. Made from materials. Of these studies, 28% were conducted in the 200-240 nm range, 27% were conducted in the 240-300 nm range, and only 44% were conducted at >300 nm. Interpreting the results allowed us to determine the trends that pharmaceutical analysis studies will lead in the future. This study can be helpful for researchers working on pharmaceutical analysis in both the industry and academy side to predict future trends for pharmaceutical analysis.

Quantification of Arbutin and its degradation products, hydroquinone and p-Benzoquinone, in hyperpigmentation topical formulation: Effect of extraction procedure and interference assessment

The utilization of Hydroquinone (HQ) in over-the-counter skincare items is subject to restrictions. Consequently, Arbutin (AR) serves as a reliable alternative for addressing hyperpigmentation in non-prescription topical formulations. Nevertheless, AR undergoes decomposition into HQ and p-Benzoquinone (BZ) when exposed to temperature stress, ultraviolet light, or dilution in an acidic environment, all of which can induce skin toxicity. The intention of this paper is to investigate the effect of extraction procedure on the conversion of AR to HQ and or BZ and to evaluate kinetics of AR hydrolysis to HQ. Meanwhile this study aims to evaluate AR and BZ interference with the United States Pharmacopoeia (USP) identification and assessment method for HQ Hydrolytic stress during extraction conditions underwent optimization through systematic screening tests. Subsequent assessment of the residual drug and its degradation products were achieved by HPLC method. The resulting data were meticulously fitted to various kinetic models. To analyze the potential interference of AR in HQ measurement using USP method, the standard concentrations of AR and HQ were analyzed through UV–VIS spectrophotometry. For enhanced certainty, a validated HPLC method analysis was also conducted. Notably, the acid hydrolysis of AR exhibited independence from its initial concentration. So, the hydrolytic degradation of AR exhibited a Zero-order kinetic profile. Furthermore, the proven interference of AR in the UV–VIS spectrophotometry method was identified within the context of the USP method. This study successfully utilized an adopted HPLC method for the concurrent quantification of AR, HQ, and BZ. The potential interference of AR in the UV–VIS spectrophotometric assay for HQ may lead to false results especially for regulatory purposes.

An indicator color chart for quick detection of Pigment Red 53 in cosmetic products in Indonesia.

Pigment Red 53 is a dangerous synthetic dye that is often added to cosmetics, even though its use in cosmetic products has been prohibited because of possible impacts on health. Faster and more sensitive detection of Pigment Red 53 is needed for onsite analysis to protect the community from illegal cosmetics that contain the dye. Indicator color charts are a kind of analytical method that can be used to detect Pigment Red 53 in cosmetic products, including lipstick, rouge, and eyeshadow. Such charts are practical, fast, and can be used for onsite analysis. In this study, an indicator for Pigment Red 53 detection was obtained through a reagent reaction that caused a specific color change. An indicator color chart was then produced by setting out in paper form the series of colors which resulted from the reaction of specific chemical reagents and Pigment Red 53 solutions at concentrations of 10, 20, 40, 60, 80, and 100 ppm. The testing results showed that the indicator color chart may be used as an initial screening method for the detection of Pigment Red 53 in cosmetic products with a detectable minimum concentration of 10 ppm. Out of nine samples, only one (Eyeshadow 3) tested positive for Pigment Red 53. Further analysis was carried out on the indicator color chart and the results showed good agreement with TLC and UV-Vis spectrophotometry methods. The results reported in this paper demonstrate that the indicator color chart is a good prospective method for onsite analysis to detect Pigment Red 53 in cosmetic samples, with a lower detection limit compared to polymer-based indicators.

IDENTIFIKASI RHODAMIN B PADA LIPSTIK YANG BEREDAR DI KOTA BENGKULU DENGAN METODE SPEKTROFOTOMETRI UV-VISIBEL

The use of the dye rhodamine B as a color additive in cosmetics has been prohibited by the Regulation of the Head of the Food and Drug Monitoring Agency (BPOM) Number: HK.00.05.42.1018 concerning Additional Ingredients in Cosmetics, because rhodamine B has carcinogenic properties if used long term in the body. In this research, 10 lipstick samples were used purposively obtained from the market in Bengkulu City. Lipstick samples were selected based on the criteria that they did not meet BPOM distribution requirements. Determination of rhodamine B levels was carried out using the UV-Visible Spectrophotometry method. The maximum wavelength used in the identification of Rhodamine B is 554 nm. The test results of ten lipsticks showed that nine lipsticks contained rhodamine B, in lipsticks with codes L2, L3, L4, L5, L6, L7, L8, L9 and L10 with a percentage of 0.0058%-0.0242%, and only one sample that was identified negative, L1 with an absorbance value of 0.364 with a percentage of 0%. In this study it can be concluded that there were 9 samples of 10 lipstick samples that were identified as containing Rhodamine B quantitatively.&#x0D;

Physical Quality Evaluation and Determination of SPF (Sun Protection Factor) Value of Forest Coconut Oil Lotion (Pandanus julianettii Martelli) from Papua

Forest coconut fruit contains double-bonded fat or omega 3-6, tocopherol, or vitamin E. Vitamin E and some compounds such as flavonoids, tannins, and anthraquinone have potential as sunscreens. This study aims to evaluate the physical quality and determine the SPF value in forest coconut oil lotion analyzed in vitro. Physical quality evaluation includes organoleptic tests, homogeneity, spreadability, adhesion, pH, and the determination of SPF values by the UV-Vis spectrophotometry method. The results showed that each formula, namely FI (2%), FII (4%), and FIII (6%), was physically stable. The lotion preparation in each formula, namely FI (2%), FII (4%), and FIII (6%), has SPF values of 1.95, 1.57, and 1.19, respectively.

Identification of Formalin in Raw Tofu from Sidenreng Rappang District Traditional Markets

Formalin (Formaldehyde) is a chemical compound whose use is prohibited as a preservative in food additives. This study aims to analyze the content of formalin (Formaldehyde) in raw tofu circulating in Sidenreng Rappang district. The test method used was by qualitative analysis using Schiff reagent and KMnO4. The quantitative analysis using the UV-Vis spectrophotometry method. The test results used Schiff reagent and KMnO4, the four tofu samples contained formalin. Test results on the UV-Vis Spectrophotometry method at a wavelength of 555 nm, obtained formalin levels in sample with sample code A = 1.130%, sample code B = 1.826%, sample code C = 1.84%. It can be concluded that in the formaldehyde qualitative analysis test on raw tofu, the three samples positively contained formaldehyde. As well as the results of the measurement of the three positive samples containing formaldehyde using a UV-Vis spectrophotometer.

Determination of Catechin Content in Green and Black Tea (Camellia sinensis L.) by UV-Vis Spectrophotometric Method

Tea is one of the most commonly consumed beverages around the world. The chemical compounds contained in tea have a good effect on health because they contain polyphenolic compounds, catechins. This study examined the catechin compound content of black tea and green tea from Karanganyar (A) and Malang (B). This research aims to determine the differences in catechin levels in black and green tea types in Malang, East Java, and Karanganyar, Central Java plantations. The research method used was experimental. Identification of catechin content was done using UV-Vis Spectrophotometry method. Tea is extracted by brewing, 4 grams of green tea is brewed using distilled water for 6 minutes, while 5.6 grams of black tea is brewed using distilled water for 10 minutes with the comparison used is pure catechin solution. The results obtained for green tea Karanganyar (A) had a catechin content of 3.61±0.083%, green tea Malang (B) had a level of 2.97±0.037%, black tea Karanganyar (A) had a level of 3.14±0.065%, and black tea Malang (B) had a level of 1.60±0.009%. It is concluded that the catechin content of green tea is higher than black tea, and of the two samples, the high catechin content comes from Karanganyar (A). Keywords: catechins, green tea, black tea, malang, karanganyar

Structural and Optical Properties of Interfacial InSe Thin Film.

This study presents a comprehensive investigation of the optical and structural characteristics of the indium selenide (InSe) film prepared on a glass substrate. The structural characteristics of the InSe film were analyzed using characterization techniques including X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray spectroscopy while the UV-vis spectrophotometry method was used in the spectral range between 500 and 1000 nm to examine the optical characteristics. Thus, the UV-vis spectroscopic data were used to extract several optical parameters including extinction coefficient (k), optical band gap (Eg), refractive index (n), absorption coefficient (α), and optical conductivity (σopt). The optical transition of InSe was found as a direct transition. However, the optical analysis of this study has revealed that the InSe film has the potential to be used in various optoelectronic and photovoltaic applications.

Perbandingan Flavonoid Total pada Ekstrak Biji Pepaya (Carica papaya L.) Varietas Bangkok dan California

Barely used by the community, papaya seeds (Carica papaya L.) astonishingly contain flavonoid compounds that can be turned into antioxidants to counter free radicals. However, the amount of flavonoids in papaya depends on where it grows. Types of papaya (Carica papaya L.) that are often grown and consumed by the Gorontalo community are Bangkok and California varieties. Therefore, this study aims to analyze the levels of flavonoid compounds in papaya seeds (Carica papaya L.) of Bangkok and California varieties grown in Gorontalo province using UV-Vis spectrophotometry instruments. Qualitative analysis in this study is conducted through phytochemical screening methods. Meanwhile, the quantitavie analysis uses the UV-Vis spectrophotometry method at 419 nm wavelength with quercetin’s standard comparison solution. The result discloses that highest average flavonoid compound of papaya seed extract (Carica papaya L.) is in Bangkok variety with 10,197% w/v, followed by the California variety with 3,475% w/v. Bangkok and California papaya seed extracts have met validation standards with correlation coefficient values of 0,9991, regression function coefficient values (Vx0) 0f 2,1%, %retrieval values of Bangkok papaya seed extract is 100,05% and California is 101, 29%, RSD% values for Bangkok papaya seed ethanol extract is 1,775% and California is 1,348%.

Comparison of Vitamin C Levels in Rose Kombucha (Rosa hybrida) during Storage

Kombucha is a drink made from tea and sugar fermented by SCOBY. One of the ingredients in kombucha that is good for the body is vitamin C. Vitamin C can increase immunity and prevent the body from free radicals. Vitamin C in kombucha is formed through a fermentation process by remodeling glucose. This research aimed to determine the comparison of vitamin C levels in kombucha with 3 and 6 days shelf life. Measurement of vitamin C levels using the UV-Vis spectrophotometry method by measuring absorbance at a maximum wavelength of 248 nm which is then calculated using a linear regression equation and measuring the pH of the preparation. Sampling by pipetting as much as 10 ml kombucha then put into a 100 ml measuring flask and add aquadest up to the mark. The results of the research showed that vitamin C levels in rose kombucha after being stored for 3 days decreased 5.05% and after being stored for 6 days, vitamin C increased by 5.32%. Therefore, the pH of rose flower kombucha also decreased after 7 days of fermentation but stays constant after being stored for 3 and 6 days (pH 4).

Analisis Kandungan Asam Retinoat Pada Krim Anti Jerawat Di Toko Kosmetik X Pasar Jodoh

Growth from adolescence to adulthood is a transition that causes many hormonal changes, both physical, psychological and social. This change will cause health problems, one of which is on the face, namely Acne vulgaris (AV). Retinoic acid is one of the chemicals that is prohibited for use because it can cause dry skin, burning, teratogenicity (fetal defects)0and skin cancer. The aim of this research was to find out whether the acne cream in the cosmetic shop x Pasar Jodoh contained retinoic acid, and to determine the levels of retinoic acid in the samples. There were 10 acne cream samples studied. The qualitative test of retinoic acid uses the thin layer chromatography (TLC) method observed under UV 254 light. And the quantitative test uses the UV-Vis Spectrophotometry method. Spectrophotometric determination of retinoic acid levels at a wavelength of 340 nm. The results0obtained by the thin layer chromatography (TLC) method were that sample B was positive for retinoic acid and the results from the UV-Vis spectrophotometry0method were 0.0005%.

Continuous Flow Injection Analysis Method for the Determination of a Drug Diphenhydramine Hydrochloride by Using Phosphomolybdic Acid

The study presented a new method for determining diphenhydramine hydrochloride (DPH) in its pure form and some pharmaceutical preparations, within CFIA technique. The method is simple, fast, sensitive, easy to operate, and of a low cost. It is based on the reaction of DPH with phosphomolybdic acid (PMA), in aqueous media, forming a white, slightly yellowish precipitate. The precipitate formed was studied using an Ayah 6S×1-ST-2D solar cell CFI analyzer, by the reflection of incident light off the surfaces of the deposited particles at (0–180°), expressed as the response measured in (mV) . Some chemical and physical variables were studied to provide optimal conditions for the study. The linear range was 0.07-9 mmol/L and it had a correlation coefficient (r) with a value of (0.9998). The limit of detection (L.O.D.) of the new method was 729.55 ng/sample, calculated by gradually diluting the lower limit of the concentration in the linear range (0.07 mmol/L). The % RSD was less than 0.2% for a concentration of 0.1, 4.0 and 10.0 mmol/L of DPH for n = 8. The method was successfully applied in the determination of DPH in three samples from three different pharmaceutical production companies. Using the standard addition method, the new method was compared with the UV-Vis spectrophotometry method at λmax = 258 nm. Both T-test and F-test. The results of the two tests showed no significant difference at a confidence level (95%).

Analysis of Hydroquinone in Face Whitening Cream by UV-Vis Spectrophotometry and GC-MS Spectrometry

The analytical method to determine the compound content in both food and cosmetic products must be carried out carefully to ensure the validity of the analysis results. Hydroquinone has been widely found to be used as an active whitening agent in whitening cream. Technological advances have led to illegal whitening creams becoming more accessible online. This study aims to examine the hydroquinone content in the whitening samples cream purchased from e-commerce by various analytical methods, such as FeCl3 reagent, cerimetric titration, UV-Vis spectrophotometry, and GC-MS spectrometer compared to the positive control hydroquinone. The results of the hydroquinone content by cerimetric titration and UV-Vis spectrophotometry methods were calculated quantitatively. Furthermore, the result compared through statistical tests showed a consistent hydroquinone content for both methods. Contrariwise, the GC-MS method showed negative results due to differences in the extraction process and solvent solubility. Based on the result obtained, can be concluded that all samples of whitening cream did not meet the requirements of BPOM No. 23 of 2019.

Atomically-dispersed manganese anchored on B,N co-doped carbon for the sensitive electrochemical detection of levodopa

Metal single-atom catalysts offer the dual advantages of high electrochemical activity and near 100 % metal atom utilization, leading to their potential use in low-cost electrochemical sensor development. Herein, a novel electrocatalyst comprising atomically-dispersed Mn on B,N co-doped bamboo-derived carbon (MnSAs-BN-BC) was synthesized via a facile pyrolysis procedure. A high dispersion of Mn single atoms in MnSAs-BN-BC was confirmed by aberration-corrected transmission electron microscopy and elemental mapping. The Mn loading in the MnSAs-BN-BC determined by inductively coupled plasma mass spectrometry was 255 mg kg−1. MnSAs-BN-BC displayed outstanding electrocatalytic performance for levodopa (LD) oxidation, allowing a robust electrochemical sensing platform for LD detection to be established. The MnSAs-BN-BC/GCE sensing platform offered a wide LD detection range (concentrations from 2 to 683 µM) and a very low limit of detection (LOD) of 0.45 µM, outperforming almost all electrochemical sensors reported to date for LD sensing. The MnSAs-BN-BC/GCE platform also featured outstanding repeatability, reproducibility, selectivity, and stability. The as-developed sensing platform was successfully applied to LD quantification in commercial tablets with satisfactory recoveries (85.2–102.4 %), with the analytical precision of method validated against a traditional UV–vis spectrophotometry method. Density functional theory (DFT) calculations showed that Mn single atom sites lowered the reaction energy barrier for LD oxidation, with the favorable d-band center position of Mn single atom sites in MnSAs-BN-BC contributing to the enhanced LD sensing performance. This work encourages the use of single-atom metal catalysts in design of high-performance electrochemical sensors for the rapid detection of LD.

Preparation of high-performance monoazo disperse dyes bearing ester groups based on benzisothiazole and their dyeing performance on polyester fabrics.

To obtain high-performance disperse dyes, a series of azo disperse dyes containing different kinds of ester groups based on benzisothiazole were synthesized by the coupling reaction of diazotization of 3-amino-5-nitro [2,1] benzisothiazole with N-substituted aniline compounds bearing different ester moieties. The structures of the synthesized dyes were evaluated using Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance techniques (1H-NMR), and MS analysis. UV-Vis spectrophotometry methods were applied to study absorption maxima, molar extinction coefficients, and solvatochromic behaviors of the dyes, and time-dependent density functional theory (TD-DFT) simulations were applied to reveal the nature of the absorption spectrum properties. Polyester fabrics were colored using a high-temperature dyeing method under pressure, and the dyed fabrics exhibited deep and bright intense blue hues. In addition, excellent fastness properties, including washing fastness, sublimation fastness, rubbing fastness, and light fastness, were achieved.

Total Metabolit Sekunder dan Uji Aktivitas Antioksidan Daun Juwet (Syzygium cumini L.) dengan Spektrofotometer Uv-Vis

The aim of this research is to determine the total amount of secondary metabolites (alkaloids, flavonoids, saponins and tannins) contained in the ethanol juice of juwet leaves (Syzygium cumini L.) using the UV-Vis spectrophotometry method and to determine the antioxidant activity of the ethanol juice of juwet leaves based on IC50 value. Determination of total levels of secondary metabolites using UV-Vis spectrophotometry and antioxidant testing using the DPPH method. The results of qualitative analysis of the ethanol juice of juwet leaves contained secondary metabolite compounds, namely flavonoids, saponins, tannins and alkaloids. The average value obtained for the level of total secondary metabolites for flavonoid compounds was 0.417% w/w., saponins 0.766 w/w., tannins 0.282 w/w and alkaloids 0.384% w/w. The results of testing the antioxidant activity of juwet leaf ethanol extract and quercetin as a comparison had antioxidant activity, the average IC50 results were 14.289 ± 0.255 ppm and 102.45 ppm, therefore they were classified as moderate antioxidants.