Cellulosic multifunctional films offer extensive versatility and hold potential for diverse applications across multiple domains. However, the majority of cellulose-based multifunctional film studies have indicated issues with inadequate stability and consistency. Herein, a cellulose-based multifunctional film with exceptional stability was fabricated using multiple chemical modifications. Firstly, the solvent exchange method was used to prepare a cellulose acetoacetate (CAA) hydrogel film. Then, the fluorescent group and gentamicin sulfate (antibacterial group) were bonded to the CAA hydrogel film through Biginelli reaction and the formation of enamine bond, respectively. The resulting film exhibited photoluminescent, high transparency (above 80%), UV-shielding (blocking 90.9% UVA), and antibacterial properties, which are also very stable to external solvents. Hence, the multifaceted films made from cellulose displayed immense promise in various areas including eco-friendly packaging for perishables, ensuring authenticity, and in manufacturing unique types of films.