A precise estimation of plant water use is crucial for efficient irrigation management and understanding of plant–water interactions. Metalized-polyethylene mulch as a ground cover, combined with irrigation management, can improve water and fertilizer use, efficiency growth, and yield of young citrus trees, compared to bare ground. Thus, a study was undertaken to (i) compare daily water use, using the stem heat balance (SHB) method on ≤4-year-old citrus trees using three irrigation methods, and (ii) to determine soil moisture content, as well as total available soil water (TASW) in the irrigated zone. Three irrigation methods, regulated deficit irrigation (RDI), conventional drip irrigation (CD), and microsprinkler irrigation (MS), were applied on two sandy soils in central and southwest Florida with treatments further split between trees grown on 2.4 m mulched beds (M) and bare ground (NM) to form a factorial design. Measurements included sap flow, canopy size, leaf area index (LAI) and soil moisture. The result of this study shows greater values for the trunk cross-sectional area, canopy volume and LAI for both mulch treatments, in comparison to bare ground treatments. The sap flow data suggest that RDI resulted in higher water use than both CD and MS. The hourly sap flow was 120, 99 and 163% greater in M-RDI than M-CD, NM-CD and NM-MS, respectively. Mulched ground cover showed a higher soil moisture average at all soil layers (8-, 15- and 45-cm depth). For instance, the mulched ground cover showed higher soil moisture (37%) at a 15 cm depth at the Entisol site, followed by 30% at a 45 cm depth and 25.3% at depth 45 cm for the Spodosol site. The TASW values varied between 100 to 136% for both mulch and bare ground treatments. The irrigation systems used showed water contents close to field capacity, indicating that water was not limiting despite the different irrigation schedules. The high-water uptake is ascribed to frequent irrigation applications and pulses, and improved water distribution in the irrigated zone as a result of the mulch. These findings demonstrate the potential of metalized-polyethylene mulch and regulated drip irrigation to enhance water use efficiency.