UV-C Light Irradiation Research Articles

Surface decoration of Nb2O5 hollow fibers by graphene oxide for enhanced photocatalytic degradation of methylene blue

AbstractThis paper describes the fabrication of ceramic Nb2O5 hollow fibers covered by a thin graphene oxide (GO) layer using the vacuum‐assisted dip‐coating process for the efficient photocatalysis degradation of methylene blue (MB) under UVC light irradiation. Pristine and GO‐coated Nb2O5 hollow fibers were evaluated for photocatalytic degradation at different initial dye concentrations and pH values. A photodegradation of 66.4% of the dye molecules was achieved using the pristine Nb2O5 hollow fibers at 57.4 ± 0.1 mg mL1, initial MB concentration of 10 mg L−1, and pH of 11. Under the same experimental conditions, the GO‐coated Nb2O5 hollow fibers degraded 100% of the MB molecules. Additionally, the GO‐coated Nb2O5 hollow fibers were successfully reused for four reaction cycles and exhibited suitable long‐term stability. Thus, the proposed ceramic Nb2O5 hollow fibers with a surface decorated by a GO layer presented suitable characteristics for use as a photocatalyst in MB degradation.

Efficient Photocatalytic Degradation of Triclosan and Methylene Blue by Synthesized Ag-Loaded ZnO under UV Light

Industrial discharge of hazardous organic and synthetic chemicals, such as antibacterials and dyes, poses severe risks to human health and the environment. This study was conducted to address the urgent need for efficient and stable zinc-oxide-based photocatalysts to degrade such pollutants. A novel approach to synthesizing silver-loaded zinc oxide (Ag@Z) catalysts was introduced by using a simple and efficient combination of hydrothermal and precipitation methods. Comprehensive characterization of Ag@Z photocatalysts was performed using XRD, XPS, Raman, UV–vis adsorption, FTIR, and SEM, revealing an enhancement of structural, optical, and morphological properties in comparison to pure zinc oxide. Notably, the 5%Ag@Z catalyst exhibited the highest degradation efficiency among the other synthesized catalysts under UV-C light irradiation, and enhanced the degradation rate of pure zinc oxide (Z) by 1.14 and 1.64 times, for Triclosan (TCS) and Methylene Blue (MB), respectively. the effect of catalyst dose and initial concentration was studied. A mechanism of degradation was proposed after investigating the effect of major reactive species. The 5%Ag@Z catalyst increased the photostability, which is a major problem of zinc oxide due to photocorrosion after reusability. We found that 50% and 74% of energy consumption for the photocatalytic degradation of TCS and MB by 5%Ag@Z, respectively, was saved in compassion with zinc oxide. The remarkable photocatalytic performance and the good recovery rate of Ag@Z photocatalysts demonstrate their high potential for photocatalytic degradation of organic contaminants in water.

Preparation, characterization, and application of gallic acid-mediated photodynamic chitosan-nanocellulose-based films

In this study, an active film composed of gallic acid (GA), chitosan (CS), and cellulose nanocrystals (CNC) was prepared using a solution casting method and synergistic photodynamic inactivation (PDI) technology. Characterization of the film showed that the CS-CNC-GA composite film had high transparency and UV-blocking ability. The addition of GA (0.2 %–1.0 %) significantly enhanced the mechanical properties, water resistance, and thermal stability of the film. The tensile strength increased up to 46.30 MPa, and the lowest water vapor permeability was 1.16 × e−12 g/(cm·s·Pa). The PDI-treated CS-CNC-GA1.0 composite film exhibited significantly enhanced antibacterial activity, with inhibition zone diameters of 31.83 mm against Staphylococcus aureus and 21.82 mm against Escherichia coli. The CS-CNC-GA composite film also showed good antioxidant activity. Additionally, the CS-CNC-GA1.0 composite film generated a large amount of singlet oxygen under UV-C light irradiation. It was found that using the CS-CNC-GA1.0 composite film for packaging and storage of oysters at 4 °C effectively delayed the increase in pH, total colony count, and lipid oxidation in oysters. In conclusion, the CS-CNC-GA composite film based on PDI technology has great potential for applications in the preservation of aquatic products.

Flexible SERS sensor based on the photodecoration of Au-NPs on Co3O4 NWs/carbon fiber cloth for the ultrasensitive detection of methylene blue in the curved fish surfaces

BackgroundDevelopment of flexible platform via the surface-enhanced Raman spectroscopy (SERS) technique has gained enormous attention as a low-cost and portable substrate for a wide range application. In particular, the fabrication of semiconductors and tuning their surface morphologies with plasmonic nanoparticles are considered to be a fascinating strategy to create numerous hotspots to yield superior SERS enhancement. ResultsThis work involved fabricating a flexible SERS active substrate using the carbon fiber cloth (CFC), which is hydrothermally grown with cobalt oxide nanowires (Co3O4 NWs) and photodecorated with plasmonic gold nanoparticles (Au-NPs) for the ultrasensitive detection of organic dye, methylene blue (MB). The proposed substrate exhibits high enhancement factor (4.5 × 1010), low limit of detection (1.42 × 10−10 M), good uniformity (6.27 %), superior reproducibility (6.30 %) and demonstrate an excellent mechanical strength up to 40 cycles towards the MB detection. The residues of the MB are directly detected on the fish surfaces by adopting a facile swab-sampling technique. Additionally, the proposed flexible SERS sensor exhibit a successful photodegradation of MB at 90 min under UVC light irradiation. Significance and noveltyThe proposed flexible SERS methodology for detecting MB in the curved surfaces exhibited a superior SERS enhancement owing to the synergistic effect raised from the Co3O4 NWs (chemical enhancement) and Au NPs (electromagnetic enhancement). These findings indicate that the CFC-based flexible SERS sensor is a promising candidate for detecting various organic pollutants in real-time and on non-planar surfaces.

Superior UVC light-mediated catalytic activity of a novel NiFe2O4@ TiO2 magnetic nanocomposite synthesized with green route using Pulicaria Gnaphalodes plant extract for enhanced photocatalytic degradation of an antibiotic in water solution

In this study, the NiFe2O4@TiO2 magnetic nanocomposite was synthesized by the green synthesis method, which is an efficient and economical method. Pulicaria Gnaphalodes plant extract was used for nanocomposite synthesis because this method is suitable for the biosynthesis of nanocomposites on a large scale, and the nanocomposite produced by plants is more stable. The efficiency of the synthesized nanocomposite was investigated for the photocatalytic degradation of Penicillin G (PNG) under UVC light irradiation in aqueous solutions. The structural characteristics of this nanocomposite were determined by field emission scanning electron microscopy, transmission electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, vibrating sample magnetometer, and dynamic light scattering. The effect of different parameters including pH, nanocomposite dose, penicillin G concentration and time were studied to reach optimum conditions. About 71% of PNG in optimal conditions (pH = 9, nanocomposite dose = 0.6 g/L, and penicillin G concentration = 10 mg/L) was decomposed. Generally, the NiFe2O4@TiO2 nanocomposite can be used as an efficient catalyst for the degradation of PNG in aqueous solutions.

Photodegradation of Wastewater Containing Organic Dyes Using Modified G-C3N4-Doped ZrO2 Nanostructures: Towards Safe Water for Human Beings

Historically, the photocatalytic efficacy of graphitic carbon nitride (g-C3N4) has been constrained by a rapid charge recombination rate and restricted sensitivity to visible light. To overcome these limitations and enhance the performance of g-C3N4, the strategic formation of heterojunctions with semiconductor materials is deemed the optimal approach. The present study employed a facile sonication-assisted pyrolysis method to synthesize a g-C3N4@ZrO2 nanocomposite photocatalyst. This hybrid material was characterized extensively using a comprehensive suite of analytical techniques, including XRD, SEM, EDX, FTIR, and UV-Vis DRS. A comparative analysis of photocatalytic applications under identical conditions was conducted for all synthesized materials, wherein they were subjected to UVc light irradiation. The photocatalytic degradation of various dye models, such as MB, EY, and a combination of dyes, was assessed using the prepared nanocomposites. The g-C3N4@ZrO2 photocatalysts showcased superior photocatalytic performance, with a particular variant, g-CNZ6, exhibiting remarkable activity. With a bandgap energy of 2.57 eV, g-CNZ6 achieved impressive degradation efficiencies of 96.5% for MB and 95.6% for EY within 40 min. Following previous studies, the superoxide radical anions (O2−. and h+) were largely accountable for the degradation of MB. Therefore, the observed efficacy of the g-C3N4@ZrO2 nanocomposite photocatalyst can be attributed to the increased generation of these reactive species.

Design, construction and robust validation of a germicidal device based on UV irradiation: a necessity for hospital disinfection in the COVID-19 era

Pandemic by SARS-CoV-2 has revealed the importance of disinfection methods due to pathogens of medical importance being detectable and infective after several hours on contaminated surfaces, including medical devices. The aim of this work was to design, construction, and validation of a UVC light irradiation system in the short wavelength region (200 to 320 nm). We studied the effective of the system through in vitro disinfection to eliminate pathogens such as SARS-CoV-2, ESKAPE bacteria and fungi in biofilm and planktonic forms. Doses of 0.25 J/cm2 (10 s of exposure to UVC light), 100% death of ESKAPE bacteria and fungi in planktonic form was observed. Through biofilm formation induction assays of these microorganisms showed resistance to treatment with UV light; however, their viability was not detected after 20 s of exposure (via confocal microscopy). For SARS-CoV-2, 100% reduction was reached after 120 s of exposure. This evidence shows the need to employ emerging methods of disinfection of surfaces and medical devices since these are potential vehicles for transmitting pathogens. The advantages of using UV light as an emergent disinfection method in the era of COVID-19 are discussed.

Chitosan-based synthesis of silver-doped tungsten oxide nanoparticles and assessment of its cytotoxicity and photocatalytic performance

In this project, pure tungsten oxide nanoparticles (WO3-NPs) and silver-doped tungsten oxide nanoparticles (Ag-doped WO3-NPs, 3, 5, and 7 % of Ag) were synthesized via the use of chitosan as the reducing and stabilizing agent in a sol–gel method. The optical and structural properties, size, and morphology of pure WO3-NPs and Ag-doped WO3-NPs were examined by UV–Vis, FT-IR, DRS, XRD, EDX, FESEM, ICP, and Raman studies. Accordingly, the XRD and FT-IR data approved the synthesis of pure and crystalline WO3-NPs and Ag-doped WO3-NPs. The evaluated cytotoxicity of nanomaterials on cancer B16F0 cell line through the MTT method exhibited their inhibitory impact on cancer cells with the IC50 of 287 and 209.6 µg/mL for WO3-NPs and Ag-doped WO3-NPs (7 %), respectively. The conducted photocatalytic tests under UV-C light irradiation indicated the effect of increasing the doping (Ag) concentration on intensifying the degradation percentage of methylene blue (MB) pigment after 260 min (83.7 % for WO3-NPs and 95.3 % for Ag-doped WO3-NPs (7 %)).

Monitoring Congo red discoloration using thermal properties in photocatalytic processes: A new approach

Waste water contaminated with azo-derived dyes is the main cause of contamination of aquatic systems. The photocatalytic process is an alternative remediation technology to counter the negative effects of dyes in aqueous solutions. In this work, the photocatalytic potential of β-PbO nanoparticles to degrade Congo red was investigated. For the first time, monitoring photocatalysis by obtaining thermal diffusivity using thermal lens spectroscopy and thermal wave resonator cavity techniques is reported. The reaction was carried out under alkaline conditions with UVC light irradiation and in the presence of lead oxide nanoparticles. It was possible to recorded a decrease of the characteristic peak of Congo red around 497 nm in the UV-Vis spectra. Likewise, a decrease in thermal diffusivity from 15.53±0.026 × 10−4 cm2•s−1 to 13.96±0.045 × 10−4 cm2•s−1 was observed as the irradiation time increased. The azo-derived dye degradation reaction follows pseudo-first-order kinetics. The model used allowed calculating an apparent rate constant of 0.0188 min−1 and half-life of the Congo red of 36.8 min−1 in the presence of orthorhombic lead oxide. The experimental conditions allowed the degradation of the dye in a percentage up to 90%.

Effects of postharvest UV-C irradiation on essential oils from leaves of Piper aduncum L. for industrial and medicinal use

Piper aduncum L. is a species known for its medicinal and pharmacological properties, cultivated due to commercial and industrial interest, especially in the production of essential oils (EOs). To explore its potential, the present study aimed to evaluate the post-harvest impact of UV-C irradiation on the metabolic profile of EOs from fresh leaves of P. aduncum cultivated in Southeastern Brazil, during different storage periods. Fresh leaves were exposed to controlled UV-C light irradiation in a chamber for 60, 120, 240, and 360 min. Subsequently, the EOs were extracted by hydrodistillation in a modified Clevenger apparatus and analyzed using GC. A non-irradiated sample was included as a control for comparison. The results revealed alterations in the relative percentage of specific chemical constituents, including arylpropanoids and sesquiterpenes, after UV-C irradiation. Remarkably, the relative percentage of the arylpropanoid dillapiole, a compound of high commercial value, increased significantly by threefold after exposure to UV-C radiation, ranging from 26.81 % (not irradiated) to 95.60 % (360 min of irradiation). This study indicates that exposure to UV-C radiation in freshly harvested P. aduncum leaves may be a promising technique for enriching the dillapiole content in the EO, with potential commercial applications and large-scale industry use.

Impact of UV-C pretreatment on β-lactoglobulin hydrolysis by trypsin: Production and bioavailability of bioactive peptides

The effects of UV-C light irradiation and low-temperature long-time (LTLT) pasteurization on protein structural changes, degree of hydrolysis (DH) by trypsin, peptide profile of tryptic hydrolysates by MALDI-TOF/TOF-MS, and bioavailability of β-lactoglobulin were compared. Compared with native or LTLT pasteurised samples, the hydrolysis rate constant of β-lactoglobulin treated with UV-C increased significantly, implying that the protein backbone cleavage sites became more accessible, whereas thermal treatment produced aggregates that impede trypsin activity. Tryptic hydrolyses of UV-C light treated β-lactoglobulin yielded more peptides and a more diverse peptide mass profile. Six bioactive peptides were revealed in β-LG tryptic hydrolysates of UV-C-treated protein; transepithelial transport in Caco-2 cell monolayers showed intermediate in vivo transport and absorption for three (β-LG f87–91, β-LG f91–99, and β-LG f158–164). The moderate allergen peptide LSFNPTQLEEQCHI β-LG was absent after tryptic hydrolysis of UV-C-treated protein, indicating that UV-C photolysis may be a useful tool for allergenicity reduction.

Graphitic carbon nitride loaded on powdered mesoporous silica nanoparticles for photocatalytic tetracycline antibiotic degradation under UV-C light irradiation

Graphitic carbon nitride loaded on powdered mesoporous silica nanoparticles for photocatalytic tetracycline antibiotic degradation under UV-C light irradiation

Density-Controlled Growth of ZnO Nanowalls for High-Performance Photocatalysts.

ZnO nanowires and nanowalls can be fabricated on the glass substrate with a ZnO seed film and low-cost aluminum (Al) foil by the aqueous solution method (ASM), respectively. The different concentrations of ZnO precursors can use to control the densities of ZnO nanowalls. In addition, FESEM, FETEM, EDS, XRD, XPS, and CL were used to evaluate the characteristics of ZnO nanowalls. The ZnO nanowalls exhibited higher photocatalytic efficiency (99.4%) than that of ZnO nanowires (53.3%) for methylene blue (MB) degradation under UVC light irradiation at the ZnO precursors of 50 mM. This result is attributed to ZnO nanowalls with Al-doped, which can improve the separation of photogenerated electron-hole pairs for enhanced photocatalytic activity. In addition, ZnO nanowalls can also reveal higher photocatalytic activity for the degradation of tetracycline capsules (TC) rather than commercial ZnO nanopowder under UVC light irradiation. The superoxide and hydroxyl radicals play essential roles in the degradation of MB and TC solutions by the radical-trapping experiment. Furthermore, the ZnO nanowalls exhibit excellent recycling and reuse capacity for up to four cycles for the degradation of MB and TC. This study highlights the potential use of ZnO nanowalls directly grown on commercial and low-cost Al foil as noble metal-free photocatalysis.

Photo-Fenton like process as polishing step of biologically co-treated olive mill wastewater for phenols removal

In this study, UVC or sunlight assisted photo-Fenton like process (PFI) using iron(III) iminodisuccinate (Fe-IDS) as catalyst was investigated for the first time as post-treatment method of a Moving Bed Biofilm Reactor (MBBR) for the treatment of olive mill wastewater (OMW). Hydraulic retention time for the MBBR process was 24 h, while the treatment time for the PFI process was 3 h under UVC or solar light irradiation. Before the MBBR treatment, OMW was centrifuged and mixed with synthetic urban wastewater to simulate conditions in a real wastewater treatment plant (WWTP). The MBBR was highly effective in reducing Biological Oxygen Demand (BOD5) and Chemical Oxygen Demand (COD) (97 and 91%, respectively), but less effective (57%) in the removal of total phenols (TPHs). PFI was optimized for TPHs removal through response surface methodology. UVC driven PFI allowed to reach higher TPHs removal than sunlight driven PFI. Fe (III) dosage was set at 2 mg/L while optimum H2O2 dosage resulted to be 144 mg/L. In these conditions, the UVC driven process allowed to obtain a TPHs removal of 99%, reaching a final TPHs concentration of 0.07 ± 0.01 mg/L.

Studies on photocatalytic removal of antibiotics, ciprofloxacin and sulfamethoxazole, by Fe3O4-ZnO-Chitosan/Alginate nanocomposite in aqueous systems

An ultraviolet-light-induced antibiotic degradation system based on nano-ZnO and Fe3O4 and chitosan/alginate nanocomposite bead assembly has been developed using a facile green-synthesis approach. In this study Camellia sinensis extract was used as reducing agent to facilitate the formation of the nanoparticles. The Fe3O4-ZnO-CS/SA nanocomposite was employed to degrade ciprofloxacin (CIP) and sulfamethoxazole (SMX) under UV-C light irradiation, and 94.77% and 93.31% degradation was achieved respectively within 180 min with the optimized conditions [pH of 4.0, antibiotic concentration of 10 mg/L, and Fe3O4-ZnO-CS/SA nanocomposite weight of 10 g and 15 g for CIP and SMX respectively]. For both the antibiotics, the pseudo-first-order model fitted well with the reaction kinetics data, while the Langmuir isotherm model showed a better fit compared to the Freundlich and Temkin models. Analyzing various characterization data reaction mechanism for CIP and SMX degradation was proposed. In addition, possible degradation pathways of CIP and SMX were proposed based on the intermediates detected by HR-LCMS analysis. Furthermore, the trapping experiments showed that superoxide (1O2) and hydroxyl (OH) radicals play major role in the photodegradation of CIP and SMX. The effects of ionic strength, natural organics and various natural water systems on degradation efficacy was investigated. The degraded products were found to show less toxicity to a model organism, a fresh water alga, compared to the parent compounds. This work provides a simple strategy to integrate CS/SA with nano-ZnO and Fe3O4 to construct effective nanocomposite photocatalyst for the degradation of different classes of antibiotics.

Enhanced photo-reactivity of polyanthracene in the VIS region.

The wavelength-dependent photo-reactivity of polyanthracene was explored upon UV-C and VIS light irradiation. The material was prepared via one-pot chemical oxidation route using FeCl3 as oxidizing agent. A decrease in surface hydrophobicity of a polyanthracene-coated poly(methylmethacrylate) substrate from 109.11° to 60.82° was observed upon UV-C exposure for 48 hrs which was attributed to increase in oxygen content at the surface, as validated by energy dispersive X-ray spectroscopy. Upon exposure to ultraviolet-visible LEDs, photo-dimerization of polyanthracene in solution occurred and was monitored using UV-VIS spectroscopy. The photo-dimer product formation decreased from 381 nm to 468 nm and was found to be higher for the polyanthracene material compared to the monomer anthracene. At 381 nm, photo-dimerization of the material was found to be approx. 4x more efficient than the non-substituted monomer counterpart. Results obtained show that photo-dimerization of polyanthracene will proceed upon exposure with visible light LEDs with reduction in efficiency at longer wavelengths. To compensate, irradiation power of the light source and irradiation time were increased.

Electro-Fenton-Based Technologies for Selectively Degrading Antibiotics in Aqueous Media

The viability of the Electro-Fenton (EF) process in the selective degradation of penicillin G (PenG) in complex solutions has been studied. The role of the anode material (boron-doped diamond (BDD) or mixed metal oxide (MMO)) and the cathode 3D support (foam or mesh), as well as the synergistic effect of UVC light irradiation (photoelectron-Fenton, PEF), have been evaluated. The results show that Pen G can be efficiently and selectively removed by EF, obtaining higher PenG removal rates when using the BDD anode (100%) than when using the MMO anode (75.5%). Additionally, mineralization is not favored under the experimental conditions tested (pH 3, 5 mA cm−2), since both aromatic and carboxylic acids accumulate in the reaction system as final products. In this regard, the EF-treated solution presents a high biological oxygen demand and a low percentage of Vibrio fischeri inhibition, which leads to high biodegradability and low toxicity of this final effluent. Furthermore, the combination with UVC radiation in the PEF process shows a clear synergistic effect on the degradation of penicillin G: 166.67% and 83.18% using MMO and BBD anodes, respectively. The specific energy required to attain the complete removal of PenG and high inhibition of the antibiotic effect is less than 0.05 Ah dm−3. This confirms that PEF can be efficiently used as a pretreatment of conventional wastewater treatment plants to decrease the chemical risk of complex solutions polluted with antibiotics.

O3/H2O2 and UV-C light irradiation treatment of oil sands process water

The oil sands industry generates large volumes of oil sands process water (OSPW). There is an urgent need for OSPW treatment to reduce process water inventories and to support current reclamation approaches. This study discusses how efficient ozone (O3)-based combined advanced oxidation processes (AOPs), including hydrogen peroxide (H2O2) and UV-C, are at achieving mineralization while reducing the toxicity arising from such organic components as naphthenic acids (NAs) in OSPW. The results showed that the dissolved organic carbon (DOC) removals of 45%, 84%, 84% and 98%, obtained after 90-min treatments with O3, O3/H2O2, UVC/O3 and UVC/O3/H2O2, respectively, at a production rate of 6 g/L·h O3 were considerably higher than at lower O3 production rates. The acute toxicity on Vibrio fischeri was significantly reduced by all the treatments, which explains the high percentages of NA removal (up to 99% as confirmed by UPLC-QTOF-HRMS.) Mineralization (expressed as DOC removal) was highest with UVC/O3/H2O2 at ca. 2 mg C/L in the treated effluent, which means that it could be used as cooling/boiling process water in bitumen upgrading units. However, considering the energy demand of the treatments tested, the treatment using O3/H2O2 was found to be the most realistic for large-scale applications.

Textural/structural evolution of cube/cauliflower-like MgSn(OH)6 nanophotocatalyst with excellent photocatalytic degradation of toxic dye pollutants

In this research, Mg–Sn hydroxide (MgSn(OH)6) nano semiconductor was synthesized using three different routes to investigate the texture/structure of MgSn(OH)6 on the photocatalytic properties of MgSn(OH)6 in RhB degradation. The nano semiconductors were characterized using XRD, FE-SEM, TEM, EDX, BET-BJH, DRS, PL, and the pHpzc. Morphological analyses for sono-hydrothermal sample showed 3D cubic microstructure. However, 3D cauliflower-like morphology was observed in the other samples. XRD analysis demonstrated the higher crystallinity of the sono-hydrothermal sample. BET-BJH analysis showed that the specific surface area and pore volume of this sample were 138.7 m2/g and 0.111 cm3/g, respectively. DRS analysis showed band-gap energy of about 4.1eV for all samples. Photocatalytic degradation experiments were conducted under UV-C light irradiation. The results demonstrated the advantage of the sono-hydrothermal sample owing to the cubic morphology, higher crystallinity and SBET, and lower recombination rate of the charge carriers. The photocatalytic degradation performance of the sample was evaluated in degradation of RhB, AO7, MO, and MB, which were 96, 93, 60, and 40%, respectively. The reusability study of sono-hydrothermal and hydrothermal samples was conducted in which the sono-hydrothermal sample showed higher efficiency and stability in four successive runs.

Effect of a UV-C Automatic Last-Generation Mobile Robotic System on Multi-Drug Resistant Pathogens.

Background: Healthcare-associated infections caused by multi-drug resistant (MDR) pathogens are associated with increased mortality and morbidity among hospitalized patients. Inanimate surfaces, and in particular high-touch surfaces, have often been described as the source for outbreaks of nosocomial infections. The present work aimed to evaluate the efficacy of a last-generation mobile (robotic) irradiation UV-C light device R2S on MDR microorganisms in inanimate surfaces and its translation to hospital disinfection. Methods: The efficacy of R2S system was evaluated in environmental high-touch surfaces of two separate outpatient rooms of Perugia Hospital in Italy. The static UV-C irradiation effect was investigated on both the bacterial growth of S. aureus, MRSA, P. aeruginosa, and K. pneumoniae KPC and photoreactivation. The antimicrobial activity was also tested on different surfaces, including glass, steel, and plastic. Results: In the environmental tests, the R2S system decreased the number of bacteria, molds, and yeasts of each high-touch spot surface (HTSs) compared with manual sanitization. UV-C light irradiation significantly inhibits in vitro bacterial growth, also preventing photoreactivation. UV-C light bactericidal activity on MDR microorganisms is affected by the type of materials of inanimate surfaces. Conclusions: The last-generation mobile R2S system is a more reliable sanitizing procedure compared with its manual counterpart.