Utilization Of Natural Enemies Research Articles

N-octyl acrylate is a candidate sex pheromone component involved in courtship in parasitoid wasp Microplitis mediator.

Sex pheromones are considered to play critical roles in partner communication of most parasitic Hymenoptera. However, the identification of sex pheromone components remains limited to a few families of parasitoid wasps. In this study, we functionally characterized a candidate sex pheromone component in Microplitis mediator (Hymenoptera: Braconidae), a solitary parasitoid of Noctuidae insects. We found that the body surface extract from female wasps could significantly stimulate courtship behavior of males. Gas chromatography-electroantennographic detection (GC-EAD) analysis revealed that a candidate semiochemical from extract triggered significant electrophysiological response of antennae of males. By performing gas chromatography-mass spectrometer (GC-MS) measurement, GC-EAD active compound was identified as n-octyl acrylate, a candidate sex pheromone component in female M. mediator. In electroantennogram (EAG) tests, antennae of male wasps showed significantly higher electrophysiological responses to n-octyl acrylate than those of females. Y-tube olfactometer assays indicated that male wasps significantly chose n-octyl acrylate compared with the control. Furthermore, male wasps showed a remarkable preference for n-octyl acrylate in a simulated field condition behavioral trial; simultaneously, n-octyl acrylate standard could also trigger significant courtship behavior in males. We propose that n-octyl acrylate, as a candidate vital sex pheromone component, could be utilized to design behavioral regulators of M. mediator to implement the protection and utilization of natural enemies.

The role of combination insectary plants and botanical pesticides in increasing arthropod populations of natural enemies and suppressing populations of s. frugiperda in corn

Spodoptera frugiperda is the main pest of corn with a high level of attack intensity. Utilization of natural enemies both predators and parasitoids can be lured using zinnia flowers could be an effective control technique in suppressing armyworm pest populations. Pests hate the smells and taste of botanical insecticides a mixed of maja fruit and giant milkweed leaves which contain secondary metabolites such as volatile, pectin, saponins and tannins. This study aims to determine the effect of zinnia flowers and the application of botanical insecticides in suppressing armyworm populations. This research was carried out in Padakkalawa, Mattirobulu District, Pinrang Regency and Universitas Hasanuddin’s Laboratory of Insect and Plant Disease Relations. The research observed pest intensity, larval population and natural enemies. The results showed that the highest attack intensity was observed in the control treatment with an average of 12.59%/plant. The highest observed larval population was in the control with an average of 2.13 individuals/plant. In observing natural enemy arthropods in all observation techniques, the highest population of natural enemy arthropods was in the combination treatment of zinnia flowers and botanical insecticides with the most species being Lasius niger or ants which was significantly different from the control treatment. The results showed that the combination of insect plants and the application of botanical pesticides had an effect on the population and attack intensity of S. frugiperda larvae and populations of natural enemy arthropods.

POTENSI PENGENDALIAN HAYATI HAMA SPODOPTERA FRUGIPERDA UNTUK KEBERLANJUTAN PRODUKSI JAGUNG

The armyworm Spodoptera frugiperda, which damages corn plants, is one of the invasive pests. The armyworm S. frugiperda damages corn plants by eating leaf tissue from one side so that the remaining epidermal tissue then makes holes in the leaves and eats from the edge of the leaf to the inside, which causes perforation in the leaves. Control efforts using insecticides have not been practical due to the feeding behaviour of the second and third instar larvae that settle on the inside of the leaves under protected conditions. Therefore, it is necessary to carry out other control techniques that can suppress the development of environmentally friendly S. frugiperda by considering economic and ecological aspects, namely biological control.
 Biological control optimizes the role of natural enemies as biological control agents in efforts to manage pest populations which are part of the chain in agroecosystems. Utilization of natural enemies, parasitoids, predators, and pathogen, can suppress the development of the S. frugiperda pest in corn, reducing the use of synthetic pesticides. Natural enemies of S. frugiperda include the egg parasitoid Telenomus sp. and Trichogramma sp.; larva parasitoid Glyptapanteles creatonoti (Viereck), solitary larval parasitoid Campoletis chlorideae Uchida, and parasitoid larvae of the order Hymenoptera: Ichneumonidae: Ichneumoninae and G. creatonoti. as well as an entomopathogenic species, Nomuraea rileyi (Farl.) Samson. Natural enemies can control S. frugiperda pests to save sustainable maize yields based on the basic principles of sustainable agriculture models. Agroecological steps are taken to reduce the population of S. frugiperda in corn plants by:/ (1) sustainable management of soil fertility, primarily actions that maintain or store soil organic carbon; (2) intercropping with properly selected companion plants; and (3) diversification of the agricultural environment through the management of (semi) natural habitats at various spatial scales.

Group Participation in Integrated Pest Control Through the Ultilization of Trichogramma sp in Rice Business in Samarang District, Garut Regency

One way to reduce the negative impact of pesticide use on rice plants is to carry out integrated pest control. As for pest control efforts that can be done is to utilize physical plant-disturbing organisms, namely natural enemies. This study was conducted to determine the level of farmer group participation in integrated pest control through the use of Trichogramma sp in rice farming, analyze the characteristics, external factors and IPM principles on the level of group participation in integrated pest control through the use of Trichogramma sp in rice plants and develop an extension strategy to increase participation. farmers. Based on the results of research from 68 respondents that the participation rate of farmer groups at the planning stage was 51.5% (medium), the implementation stage was 64.7% (medium) at the evaluation stage was 63.2% (medium). Factors that partially influence participation are length of farming (0.021), institutional role (0.032), social and cultural environment (0.000), utilization of natural enemies (0.001), routine monitoring (0.000), and farmers as IPM experts ( 0.037). Based on the high value beta coefficient taken from indicators that have a significant effect on the participation variable, a strategy is formulated to increase farmer group participation through strengthening the social and cultural environment, strengthening the frequency of monitoring land and plants so that farmers know the conditions and needs of these plants.

Evaluation of the close degree of the spatial relationship between natural enemies of tea gardens and Frankliniella intonsa based on variance analysis of cluster samples

AbstractThe aim of this paper is to clarify the difference of spatial closeness between natural enemies and Frankliniella intonsa, and to provide a scientific basis for rational protection and utilization of natural enemies. This paper applied the block‐sample square difference analysis method, Gray correlation degree method, the aggregation intensity index method and index method to study the difference in the closeness of the spatial relationship between F. intonsa and natural enemies when the number of F. intonsa and their natural enemies was the highest and the minimum area occupied by F. intonsa individuals and colonies in five tea plantations in Hefei, Anhui province in 2021 and four tea plantations in 2020. The results were as follows: 1) Tetragnatha squamata Karsch, Xysticus ephippiafus, Erigonidium graminicolum and Theridion Octomaculatum were the top four natural enemies closely related to F. intonsa in 2021. The top three natural enemies closely related to F. intonsa in 2020 were Tetragnatha squamata Karsch, Xysticus ephippiafus and Theridion Octomaculatum. Two years ago, Tetragnatha squamata Karsch and Xysticus ephippiafus were the same. 2) Cluster size did not change the distribution patterns of natural enemies and F. intonsa. 3) The aggregation of F. intonsa was caused by their own causes or some environmental factors, and the aggregation of natural enemies was caused by environmental factors. 4) In 2021, the minimum area occupied by individuals and groups of F. intonsa in tea gardens was 2m2, and in 2020, it was 8 m2.

Utilization of Endophytes Fungi from Jatropha Leaves (Jatropha curcas L.) Against Fusarium oxysporum Causing Tuber Rot Disease Onion (Allium cepa var. Ascalonicum (L.) Backer)

Tuber rot is one of the important diseases in onion caused by Fusarium oxysporum. Endophytes are biological agents that are currently widely used in controlling plant diseases. Excessive use of pesticides and continuously has shown negative impacts such as resurgence, resistance to pests and pathogens, and the death of natural enemies. Currently, pests and pathogens control effort are directed at the utilization of natural enemies or better known as biological control. Endophyte is a microorganism that grows in plant tissues without causing symptoms. Endophytes allegedly capable of producing a variety of phytochemical compounds generated by their host. The leaves of Jatropha (Jatropha curcas L.) is one part of the plant that contains secondary metabolites.Phytochemical tests on the Jatropha show that Jatropha contains alkaloids, flavonoids, and saponins that are an antimicrobial potential of plant-pathogen control agents. This study aimed to determine the effect of some Endophytes isolates and their impact on the growth of F. oxysporum in vitro in onion.Exploration (endophyte isolation from Jatropha healthy leaves) and observation (antagonist test of Endophytes of the Jatropha leaves) method used in this study was a Completely Randomized Design (CRD) with a single factor (10 treatment) repeated three times. There were 14 endophytes isolated from Jatropha leaves with a growth ratio of 4.5 cm/2 days. The best treatment to inhibit the growth of F. oxysporum in vitro is Jc5, Jc8, Jc10.

TINGKAT ADOPSI TEKNOLOGI PENGENDALIAN HAMA TERPADU PETANI PADI (Studi Kasus Di Kecamatan Sungai Kakap Kabupaten Kubu Raya)

Plant Pest Organisms disruption on rice cultivation is increasing, the control carried out by the farmers have not succeeded to the maximum, the effects of climate change are difficult to predict. Both can be very threatening productivity of rice plants in the district Kakap River. Control methods that have been recommended by the PP. No.6 1995, protection of plants which is the basic foundation for addressing attacks Plant Pest Organisms Disorder with Integrated Pest Management system. The application of technology by farmers properly and sustainably need to be known, the study aims: (1) to determine the level of adoption of these technologies participating farmers. (2) to determine the factors associated with the level of farmer adoption of Integrated Pest Management technology participants. The elements of Integrated Pest Management (IPM) technology are; utilization of natural enemies, agroecosystem management, mechanical physical control, pesticide control and control the group. Variables related to the level of adoption of Integrated Pest Management (IPM) technology participating farmers are; farmers age, formal education, non-formal education, farming experience, vast arable land, the ownership status of claim, cosmopolitan and income of farmer. The descriptive method of research that studies the problems of the farming community, relations activities, attitudes, outlook and ongoing process. Population census method used by the four groups of farmers who have been following the activities of the SL-IPM as many as 100 people, to analyze the data using Chi Square test. The results of the study generally indicates the level of participant farmer adoption of IPM technologies in the medium categories means participant farmers of IPM technology is not according with the recommendation. So the IPM technology training through the SL-IPM still needed to farmer groups. Keywords: Plant Pest Organisms disruption, level of adoption, IPM technology

EFIKASI PESTISIDA NABATI DARI TANAMAN SELA JAMBU METE DAN JAMUR ENTOMOPATOGEN SYNNEMATIUM SP. UNTUK MENGENDALIKAN WERENG PUCUK METE (SANURUS INDECORA)

Cashew plant is the one of export commodity that has high economic value compared to the other commodities. Sanurus indecora is one of major pest in cashew, causing decreased quality and quantity of products. The aims of this experiments were to the develop integrated pest management of S. indecora, by: (a) utilization and enviroment manipulation of cashew plantation using intercropping plants to control S. indicora, (b) utilization of natural enemies, specially Synnematium sp., to control S. indecora. The experiment consisted of : (1) the effect of intercropping plant extracts as botanical pesticide on S. indecora mortality at laboratory, (2) the effect of medium substances on Synnematium sp. growth, as well as the effect of concentration of Synnematium sp. suspension on S. indecora mortality at laboratory. Botanical pesticides that were used in this experiment was made from extraction of intercropping plants: extraction of sesame seed, of cassava leaves and pineapple fruit. All experiments were arranged in complete randomized design. The results showed that 0.5 mg/10 mL water of sesame seed extract, 0.1 mg/10 mL water of cassava leaves extract and 2.0 mg/10 mL pineapple fruit extract concentrations resulted average 76.67%, 66.67%, and 63.33% S. indecora mortalities at 24 hours after application, respectively. The second experiment showed that the growth medium containing rice straw resulted the best growth of Synnematium sp. colonies than the other treatments and 0.1 g/mL water of Synnematium sp. mycelium resulted the high mortality (57,5%) at 5 days after application.