Utilization Of Creatine Phosphate Research Articles

Intracellular alterations of the creatine kinase isoforms in brains of schizophrenic patients

Postmortem brain tissues of schizophrenic patients were found to contain 5-10 times less water-soluble creatine kinase (BB CK) and 1.5-3 times less mitochondrial creatine kinase as compared to control. The major part of BB CK in schizophrenic brain tissues, contrary to control, was found to be insoluble in water (particulate form of BB CK) and could be extracted from brain tissue with strong denaturating agents. The particulate form of BB CK did not have any enzymatic activity but activity was found after the solubilization of this isoenzyme. The observed BB CK translocation into the particulate inactive form and the decrease of mitochondrial CK content to schizophrenic brains may reflect changes in the synthesis and the utilization of creatine phosphate.

Effects of P13, a polyflavane compound, on pig myocardium ATP-synthesis, ATPases and creatine kinase

The effects of P13, were examined on energy stores of the pig myocardial cell. The synthesis of ATP by oxidative phosphorylation was not modified in intact mitochondria. ATP hydrolysis by the various cell ATPases was inhibited in varying degrees: sarcolemma > sarcoplasmic reticulum > mitochondria > myosin. The inhibition depended on the accessibility of P13 to the enzyme since P13 strongly inhibited submitochondrial particle ATPase activity without modifying the activity in intact mitochondria. P13 acted competitively with respect to ATP, but a mixed type inhibition was observed when the enzyme was bound to a membrane. Myosin ATPase activity was almost unaffected by P13. Creatine phosphate utilization by creatine kinase was strongly inhibited by P13. Therefore. P13 helps to maintain high levels of energy stores: ATP and creatine phosphate.

The Mechanochemistry of Cardiac Muscle

The utilization of creatine phosphate (CP) and adenosine triphosphate (ATP) was studied in the iodoacetate (IAA) and nitrogen (N(2))-treated cat papillary muscle. Under these conditions the net production of ATP does not occur, and the net utilization of ATP is reflected in a fall in CP concentration. The rate of energy utilization of the IAA-N(2)-treated cat papillary muscle resting without tension was 0.68 micromole CP/g/min. This rate was increased to 1.07 micromole/g/min when muscles were passively stretched with 2 g of tension. In a series of isometrically contracting muscles CP utilization was found to be proportional to the number of activations and the summated contractile element work. These rates of CP utilization were 0.083 micromole/g/activation and 0.0059 micromole/g-cm of work. The calculated mechanochemical coupling efficiency was 33%.

On the proposed origin of creatinine from creatine phosphate

1. 1. Solutions containing extracts of normal rabbit muscle, glucose 1-phosphate, creatine phosphate, and cysteine were incubated and analyzed for creatine and creatinine formation and creatine phosphate utilization. 2. 2. The muscle extract catalyzed the conversion of creatine phosphate to creatine without any detectable formation of creatinine.

Creatine-phosphate utilization by muscle extracts of rabbits on vit. E-deficient diets.

Creatine-phosphate utilization by muscle extracts of rabbits on vit. E-deficient diets.

Utilization of creatine-phosphate by muscle extracts from rabbits with nutritional muscular dystrophy

Utilization of creatine-phosphate by muscle extracts from rabbits with nutritional muscular dystrophy