Use Of Splicing Research Articles

The role of internal constraints and stylistic congruence on a variant's social impact

AbstractIn natural conversation, multiple factors likely impact the social force of a sociolinguistic variant, yet researchers have tended to examine individual factors in isolation. This paper considers two underexamined factors together—the role of a variable's internal constraints and the role of stylistically congruent surrounding speech—to understand their combined influence on how a single variable's realization is socially interpreted. Focusing on English variable (ING), two accent rating experiments used stimuli varying the grammatical category of (ING) words and varying the stylistic congruence (natural sentences versus spliced stimuli) between (ING) realization and sentence frames. Results indicate that listeners showed sensitivity to (ING)'s internal constraints but only when the congruence between (ING)'s realization and other cues was not disrupted by using spliced stimuli. These findings suggest that internal constraints and stylistic congruence play a role in social signaling, and have methodological implications for the use of splicing.

STaQTool: Spot tracking and quantification tool for monitoring splicing of single pre-mRNA molecules in living cells

The vast majority of human protein-coding genes contain up to 90% of non-coding sequence in the form of introns that must be removed from the primary transcripts or pre-mRNAs. Diverse forms of mRNAs encoded from a single gene are created by the differential use of splice sites and alternative splicing is rapidly evolving. Although the kinetic properties of splicing are thought to be critical for proofreading and regulatory mechanisms, tools for making direct experimental measurements of splicing rates are still limited. We recently developed a strategy that permits real-time imaging of fluorescent-labelled introns in single pre-mRNA molecules. Here we describe the software tool that we created for automatic tracking and quantification of intronic fluorescence at the site of transcription in live human cells.

Design and development of H.264-based embedded network video server

This paper developed a network video server based on Freescale video processing chip IMX27 running on the Linux operating environment,and short connection was used to complete the interaction between the server and clients,which effectively saved system resources and reduced the CPU utilization of the sever.Moreover,in order to obtain high quality video images,the server adopted an adaptive de-interlacing algorithm based on the use of splicing and interpolation of static and moving picture respectively to realize de-interlacing of the video image,therefore the integrity of the video data was guaranteed and the video clarity was improved.In the end,an application case of the server was given,which achieved the desired designed goal.

Genetic content and evolution of adenoviruses.

This review provides an update of the genetic content, phylogeny and evolution of the family Adenoviridae. An appraisal of the condition of adenovirus genomics highlights the need to ensure that public sequence information is interpreted accurately. To this end, all complete genome sequences available have been reannotated. Adenoviruses fall into four recognized genera, plus possibly a fifth, which have apparently evolved with their vertebrate hosts, but have also engaged in a number of interspecies transmission events. Genes inherited by all modern adenoviruses from their common ancestor are located centrally in the genome and are involved in replication and packaging of viral DNA and formation and structure of the virion. Additional niche-specific genes have accumulated in each lineage, mostly near the genome termini. Capture and duplication of genes in the setting of a 'leader-exon structure', which results from widespread use of splicing, appear to have been central to adenovirus evolution. The antiquity of the pre-vertebrate lineages that ultimately gave rise to the Adenoviridae is illustrated by morphological similarities between adenoviruses and bacteriophages, and by use of a protein-primed DNA replication strategy by adenoviruses, certain bacteria and bacteriophages, and linear plasmids of fungi and plants.

Prostanoid receptor heterogeneity through alternative mRNA splicing

Prostaglandin (PG) and thromboxane (TX) receptors are G-protein coupled receptors that mediate the physiological actions of the five principal prostanoid metabolites: PGD 2, PGE 2, PGF 2α, PGI 2 (prostacyclin) and TXA 2. Five major subdivisions of the prostanoid receptor family have been defined pharmacologically which correspond to each of the metabolites as follows: DP, EP, FP IP and TP. The EP receptors have been further classified pharmacologically into the EP 1, EP 2, EP 3 and EP 4 subtypes. Molecular biological studies have resulted in the cloning of cDNA's encoding all of these prostanoid receptors. In addition, the cloning of these receptors has revealed further heterogeneity through the use of alternative mRNA splicing. Specifically, mRNA splice variants have been identified for the EP 1, EP 3, FP and TP receptors. Interestingly, except for the EP 1 receptors, the mechanisms giving rise to these receptor isoforms involves the use of splice sites located in the cytoplasmic carboxyl termini of these receptors. Thus, the eight human EP 3 isoforms that have been identified are otherwise identical except for their carboxyl termini. Similarly, the optional use of a potential splice site encoding the carboxyl terminus gives rise to each of the two FP and TP receptor isoforms. Because the carboxyl termini of G-protein coupled receptors are generally implicated in interactions with G-proteins, it is not surprising that these receptor isoforms differ mainly with respect to their activation of second messenger pathways and not in their pharmacological characteristics. Differences also exist with respect to their levels of constitutive activity (e.g., in the absence of agonist) and in their desensitization.

Regulation of the expression of distinct human secreted IgE proteins produced by alternative RNA splicing

The use of splice sites for human epsilon mRNAs is tightly regulated, as the potential number of splice products far exceeds that actually produced. Our studies show that use of the epsilon alternative splices is regulated by a limited number of stimuli, and the relative production of the epsilon mRNA variants follows a developmental profile. In addition, we have found disease-related changes in the pattern of epsilon mRNA variants encoding distinctive IgE isoforms. Analysis of the biological activity of expressed recombinant IgE proteins and measurement of their levels in disease conditions will directly answer questions as to the biological relevance of the changes observed in epsilon mRNA splicing. This information will then be able to be used for rational therapeutic design interventions in IgE-mediated disorders.

Successful testing of 345-kV XLPE cables and premolded joints at IREQ

Extruded polymeric cables and accessories are an alternative to self contained fluid filled (SCFF) cables for extra-high-voltage (EHV) systems. Crosslinked polyethylene (XLPE) insulated cables have many advantages over the traditional fluid-filled pressurized cable system with the elimination of the hydraulic system and the associated equipment and complications during the installation and operation/maintenance of such systems. Other advantages of the extruded cable system arise from the new accessory technologies and the use of splices and terminations that are prefabricated and pretested in the factory and require less time to install on site. Concerns over the long term reliability of high voltage cable systems, in particular the accessories and the lack of service experience above 300 kV led to the decision of Hydro-Quebec to carry out a prequalification (long-term) test program to assess the reliability of the cable materials and to verify the cable and accessory installation methods to be employed. The cables were installed in duct banks and manholes simulating actual installation conditions used in Hydro-Quebec underground cable network. This paper describes the program and results of the prequalification tests of 345-kV XLPE cables and accessories performed at Hydro-Quebec's Research Institute (IREQ) in partnership with three international cable manufacturers, Alcatel, Fujikura and Pirelli.

Cloning of decay-accelerating factor suggests novel use of splicing to generate two proteins

Decay-accelerating factor (DAF), a glycoprotein that is anchored to the cell membrane by phosphatidylinositol, binds activated complement fragments C3b and C4b, thereby inhibiting amplification of the complement cascade on host cell membranes. Here, we report the molecular cloning of human DAF from HeLa cells. Analysis of DAF complementary DNAs revealed two classes of DAF messenger RNA, one apparently derived from the other by a splicing event that causes a coding frameshift near the C terminus. The apparent 'intron' sequence contains an Alu family member and encodes contiguous protein sequence. Two DAF proteins are therefore possible, having divergent C-terminal domains which differ in their hydrophobicity. Both mRNAs are found on polysomes, suggesting that both are translated. We propose that the major (90%) spliced DAF mRNA encodes membrane-bound DAF whereas the minor (10%) unspliced DAF mRNA may encode secreted DAF and we present expression data supporting this. The deduced DAF sequence contains four repeating units homologous to a consensus repeat found in a recently described family of complement proteins.