Use Of Spectral Filters Research Articles

Light filtration technology for sustainable microalgal biomass production

The use of native microalgal strains, which are well adapted to local environmental conditions, for sustainable biofuels production has largely been marred by photonics-related challenges. To date, most photobioreactor systems make use of artificial sources of illumination thus increasing the overall costs of biomass production. Solar energy, although sustainable and cost-effective, is difficult to manage and control. It also contains other wavelengths which are detrimental to microalgae. Thus, this study sought to make use of spectral filters for optimal outdoor algaculture. Hence, solar energy was used in wastewater-mediated algaculture of native and imported Chlorella sp. under blue, green, red and yellow coloured spectral filters. The native Chlorella sp. had the highest growth rate of 0.892 d−1 and 0.754 d−1 under green and blue coloured filters, respectively. In comparison, the imported Chlorella strain had a growth rate of 0.379 d−1 and 0.267 d−1 under green and blue filters, respectively. Both strains produced high lipid yields under the blue coloured filter, with the native and imported Chlorella strains managing lipid yields of 41.87% dry cell weight (dcw) and 32.29% dcw, respectively. The native Chlorella strain also significantly lowered (p < 0.05) the levels of total nitrogen and ammonium from wastewater with removal efficiencies of 92.17% and 44.60%, respectively, whereas the imported Chlorella strain managed a removal efficiency of 80.81% total nitrogen and 26.10% ammonium under the blue coloured filter. The results indicate that light filtration technology can be used, sustainably, in the simultaneous algaculture of native strains and remediation of wastewater.

Sleep and circadian phenotype in people without cone-mediated vision: a case series of five CNGB3 and two CNGA3 patients.

Light exposure entrains the circadian clock through the intrinsically photosensitive retinal ganglion cells, which sense light in addition to the cone and rod photoreceptors. In congenital achromatopsia (prevalence 1:30–50 000), the cone system is non-functional, resulting in severe light avoidance and photophobia at daytime light levels. How this condition affects circadian and neuroendocrine responses to light is not known. In this case series of genetically confirmed congenital achromatopsia patients (n = 7; age 30–72 years; 6 women, 1 male), we examined survey-assessed sleep/circadian phenotype, self-reported visual function, sensitivity to light and use of spectral filters that modify chronic light exposure. In all but one patient, we measured rest-activity cycles using actigraphy over 3 weeks and measured the melatonin phase angle of entrainment using the dim-light melatonin onset. Owing to their light sensitivity, congenital achromatopsia patients used filters to reduce retinal illumination. Thus, congenital achromatopsia patients experienced severely attenuated light exposure. In aggregate, we found a tendency to a late chronotype. We found regular rest-activity patterns in all patients and normal phase angles of entrainment in participants with a measurable dim-light melatonin onset. Our results reveal that a functional cone system and exposure to daytime light intensities are not necessary for regular behavioural and hormonal entrainment, even when survey-assessed sleep and circadian phenotype indicated a tendency for a late chronotype and sleep problems in our congenital achromatopsia cohort.

In situ frequency gating and beam splitting of vacuum- and extreme-ultraviolet pulses.

Monochromatization of high-harmonic sources has opened fascinating perspectives regarding time-resolved photoemission from all phases of matter. Such studies have invariably involved the use of spectral filters or spectrally dispersive optical components that are inherently lossy and technically complex. Here we present a new technique for the spectral selection of near-threshold harmonics and their spatial separation from the driving beams without any optical elements. We discover the existence of a narrow phase-matching gate resulting from the combination of the non-collinear generation geometry in an extended medium, atomic resonances and absorption. Our technique offers a filter contrast of up to 104 for the selected harmonics against the adjacent ones and offers multiple temporally synchronized beamlets in a single unified scheme. We demonstrate the selective generation of 133, 80 or 56 nm femtosecond pulses from a 400-nm driver, which is specific to the target gas. These results open new pathways towards phase-sensitive multi-pulse spectroscopy in the vacuum- and extreme-ultraviolet, and frequency-selective output coupling from enhancement cavities.

MID-IR LED-based, Photoacoustic CO2 Sensor

The technology used to implement CO2 sensors depends on the requirements in terms of sensitivity, price and robustness. The most common technology for highly sensitive tasks are based on tunable diode laser spectroscopy, while so-called non-dispersive infrared (NDIR) photometers [2] are used in less demanding scenarios such as control air conditioning systems. Most NDIR systems use thermal emitters as light source which are readily available at low cost but require compensation for cross-sensitivities toward other gas species. The detector technology employed in these systems ranges from photodiodes to thermopiles and pyroelectric detectors, all of which require the use of spectral filters to avoid cross sensitivities. Here we present a low-cost photoacoustic-based detector comprised of a microphone in a hermetically sealed chamber filled with CO2. To excite sound waves a MID-IR LED emitting radiation in the strong CO2 absorption region around 4.2μm is used for the first time.

The price for reduced light toxicity: Do endoilluminator spectral filters decrease color contrast during Brilliant Blue G–assisted chromovitrectomy?

BackgroundVitreoretinal surgeons have been slow to adopt the use of spectral filters for endoillumination to reduce retinal light toxicity. This study shows that spectral filters can be used without a loss in color contrast during brilliant blue G chromovitrectomy.MethodsTo evaluate the influence of intra operative spectral light filters on perceivable contrast during Brilliant Blue G chromovitrectomy, a prospective, observational clinical study was carried out on 59 consecutive Brilliant Blue G chromovitrectomy interventions in 59 patients admitted for macular holes, macular pucker or vitreomacular traction syndromes. Subsequent to peeling of the internal limiting membrane, six different illumination modes were enabled consecutively: mercury vapor, mercury vapor/xenon, and xenon followed by xenon combined with an amber, green or yellow spectral filter. Main outcome measure was the chromaticity spread between stained internal limiting membrane and unstained retina as a measure for the color contrast perceived by the human eye.ResultsMean chromaticity scores were similar for all light sources: mercury vapor 7.97, mercury vapor/xenon 7.96 (p = 0.96), and xenon 7.41 (p = 0.55). Compared to xenon, the additional use of endoillumination spectral filters did not change contrast recognizability: Chromaticity scores were 9.38 for the amber filter (p = 0.13), 6.63 for the green and 7.02 for the yellow filter (p = 0.37 and 0.64, respectively). When comparing the different filters head-to-head, the amber filter was superior to the green filter (p = 0.03), while the yellow was intermediate and not significantly different from either the amber (p = 0.08) or the green filter (p = 0.51).ConclusionsColor contrast perceptibility during Brilliant Blue G assisted chromovitrectomy is similar with mercury vapor, mercury vapor/xenon or xenon light sources. Spectral filters do not decrease color contrast recognizability. Head-to-head comparison shows a significant advantage for the amber over the green filter with respect to contrast generation, the yellow filter is intermediate. As spectral filters are known to greatly reduce retinal light toxicity, we suggest donor eye studies to validate whether the amber filter should be generally recommended for Brilliant Blue G chromovitrectomy.

Microwave phase shifter with controllable power response based on slow- and fast-light effects in semiconductor optical amplifiers

We suggest and experimentally demonstrate a method for increasing the tunable rf phase shift of semiconductor waveguides while at the same time enabling control of the rf power. This method is based on the use of slow- and fast-light effects in a cascade of semiconductor optical amplifiers combined with the use of spectral filtering to enhance the role of refractive index dynamics. A continuously tunable phase shift of approximately 240 degrees at a microwave frequency of 19 GHz is demonstrated in a cascade of two semiconductor optical amplifiers, while maintaining an rf power change of less than 1.6 dB. The technique is scalable to more amplifiers and should allow realization of an rf phase shift of 360 degrees.

Considerations Regarding the Use of Hyperspectral Imaging Data in Classifications of Food Products, Exemplified by Analysis of Maize Kernels

Development of robust analytical procedures is critical when using hyperspectral imaging technology in food technology and agriculture. This study used near-isogenic inbred corn lines to address two basic questions: (1) To what extent is classification accuracy increased by grinding maize kernels? (2) Can the classification accuracy of two near-isogenic inbred lines be increased by using a spectral filter to classify only certain hyperspectral profiles from each image cube? Whole kernels and ground kernels in two particle intervals, 0.250-0.354 mm (size 1) and 0.354-0.841 mm (size 2), were examined. Spectral profiles acquired from ground kernels had higher spectral repeatability than data collected from whole kernels. The classification error of discriminant functions from whole kernels was >3 times lower than that of size 1 ground particles. Applying a spectral filter to input data had negligible effect on classifications of hyperspectral profiles from whole kernels and size 2 ground particles, but for size 1 ground particles a considerable increase in accuracy was observed. Independent validation confirmed that distinction between wild type and mutant inbred maize lines could be conducted with >80% accuracy after the proposed spectral filter had been applied to hyperspectral profiles of size 1 ground particles. A combination of discriminant analysis and regression analysis could be used to accurately predict mixture ratios of the two inbred lines. The use of spectral filtering to increase the level of spectral repeatability and the use of hyperspectral imaging technology in large-scale commercial operations are discussed.

Photonic crystal fibre based all-optical modulation format conversions between NRZ and RZ with hybrid clock recovery from a PRZ signal

Several all-optical modulation format-converting schemes are described for non-return-to-zero (NRZ) and return-to-zero (RZ) modulation formats that make use of spectral filtering of either self-phase modulation (SPM) or cross-phase modulation (XPM) broadened signal spectrum in a highly-nonlinear dispersion-flattened photonic-crystal fibre. Format conversions have been performed between the most widely used modulation formats - NRZ and RZ. In addition, a hybrid clock recovery scheme is proposed to obtain the data rate of the NRZ signal for NRZ-to-RZ format conversion. All format-converting schemes are based on the extraction of the spectral components in a nonlinear phase modulation broadened signal spectrum. In NRZ-to-RZ format conversion, a periodic pulse train, at a repetition rate similar to the NRZ data rate, is used as a control that induces a nonlinear phase shift to the NRZ probe signal and broadens its spectrum. The spectral components, contributed by different time instances of the control pulse, can be extracted as the converted RZ signal output. In RZ-to-NRZ format conversion, the RZ signal serves as a control that induces a nonlinear phase shift to a continuous wave probe light, where a logic-inverted NRZ signal can be extracted by filtering out the chirped components. Format conversions between NRZ and RZ signals at 9.95328 GB/s (OC-192) are demonstrated. As the proposed optical signal-processing schemes make use of the fibre nonlinearity (SPM/XPM), it is possible to extend it to a high-speed operation <160 Gb/s. Therefore the proposed format-converting schemes can serve as a format converter between the optical time-division multiplexed networks and the wavelength division multiplexed networks

Optimal generation of pulsed entangled photon pairs

We experimentally investigate a double-pass parametric down-conversion scheme for producing pulsed, polarization-entangled photon pairs with high visibility. The amplitudes for creating photon pairs on each pass interfere to compensate for distinguishing characteristics that normally degrade two-photon visibility. The result is a high-flux source of polarization-entangled photon pulses that does not require spectral filtering. We observe quantum interference visibility of over 95% without the use of spectral filters for 200 femtosecond pulses, and up to 98.1% with 5 nm bandwidth filters.

Low-picomolar limits of detection using high-power light-emitting diodes for fluorescence

Fluorescence detectors are ever more frequently being used with light-emitting diodes (LEDs) as the light source. Technological advances in the solid-state lighting industry have produced LEDs which are also suitable tools in analytical measurements. LEDs are now available which deliver 700 mW of radiometric power. While this greater light power can increase the fluorescence signal, it is not trivial to make proper use of this light. This new generation of LEDs has a large emitting area and a highly divergent beam. This presents a classic problem in optics where one must choose between either a small focused light spot, or high light collection efficiency. We have selected for light collection efficiency, which yields a light spot somewhat larger than the emitting area of the LED. This light is focused onto a flow cell. Increasing the detector cell internal diameter (i.d.) produces gains in (sensitivity)3. However, since the detector cell i.d. is smaller than the LED spot size, scattering of excitation light towards the detector remains a significant source of background signal. This can be minimized through the use of spectral filters and spatial filters in the form of pinholes. The detector produced a limit of detection (LOD) of 3 pM, which is roughly three orders of magnitude lower than other reports of LED-based fluorescence detectors. Furthermore, this LOD comes within a factor of six of much more expensive laser-based fluorescence systems. This detector has been used to monitor a separation from a gel filtration column of fluorescently labeled BSA from residual labeling reagent. The LOD of fluorescently labeled BSA is 25 pM.

Combining classifier decisions for robust speaker identification

In this work, we combine the decisions of two classifiers as an alternative means of improving the performance of a speaker recognition system in adverse environments. The difference between these classifiers is in their feature-sets. One system is based on the popular mel-frequency cepstral coefficients (MFCC) and the other on the new parametric feature-sets (PFS) algorithm. The feature-vectors both have mel-scale spectral warping and are computed in the cepstral domain but the feature-sets differs in the use of spectral filters and compressions. The performance of the classifier is not much different in recognition rates terms but they are complementary. This shows that there is information that is not captured in the popular mel-frequency cepstral coefficients (MFCC), and the parametric feature-sets (PFS) is able to add further information for improved performance. Several ways of combining these classifiers gives significant improvements in a speaker identification task using a very large telephone degraded NTIMIT database.

The effect of spectral filters and nitrogen dose on the growth of chrysanthemum (Chrysanthemum morifolium Ramat., cv. Snowdon)

SummaryThis study has searched for interactions between the effects of nitrogen dose, applied at one of four levels, 0.316, 1.0, 3.16 and 10 mM, and four different spectral filters on the growth and development of chrysanthemums. The filters used included two controls with different overall transmissions to photosynthetically active radiation (PAR) and two further materials, which selectively filtered either red or far-red light, but with PAR transmission similar to one of the controls. Increasing the nitrogen dose to a maximum of 10 mM from the lowest level led to a four-fold increase in plant dry mass, and a near two-fold increase in plant height. The FR absorbing spectral filters produced significantly shorter plants than both controls (by ca. 10% at final flowering) and for this variable there were no interactions with nitrogen dose. At 10 mM N, the FR-absorbing material also led to an increase in leaf area compared with the R-absorbing material (by 55% after 40.d). However, a significant interaction occurred with N dose, such that the FR-absorbing filter increased leaf area only at the highest levels of N. The results are discussed in terms of the potential use of spectral filters for the control of the growth of ornamental crops.

Believable Scales and Parameterizations in a Spectral Transform Model

Abstract Through analysis of the spectra of a Dirac delta function the notion of believable and unbelievable scales in a spectral transform model is quantified. The smallest resolved local features are shown to have an average wavenumber a factor of 2 less than that of the truncation, and a half-width 1.6 times greater than that associated with the smallest-scale waves and 2.4 times the maximum grid size of a quadratic transform grid. A reassessment of the philosophy of including parameterizations is introduced, the central point of which is a separation of the parameterization and dynamical truncation scales. The use of spectral filters applied to the input and output from gridpoint physical parameterization schemes and the use of a coarse “physics” grid are proposed. Diagnostics summarizing the noise in a spectral model are introduced, and a moist baroclinic wave life-cycle simulation and a one-dimensional analog to photochemistry at the terminator are discussed to demonstrate their use.

A comparison between temporal coherence and spectral filtering techniques for signal enhancement

Temporal coherence differences between a signal of interest and background illumination can be used to enhance the contrast of the signal. A sensor system that uses this information is demonstrated for both laser and non-laser detection. The performance of this system is then compared to the use of spectral filtering alone. By employing coherence modulation, it is shown that the system gain can be improved by several orders of magnitude.