Use Of Blue Laser Research Articles

Evolution of methods of assistance in stapes surgery in patients with otosclerosis

Introduction. It is not always possible to create a perforation in the footplate of the stirrup with the help of classical microinstruments during stapedoplasty, which will exactly correspond to the required parameters, moreover, these tools can be dangerous in some cases, since it is possible to provoke the footplate to enter the ear labyrinth, mobilizing it.Aim. To determine the efficacy and safety of various methods of stapes surgery in otosclerosis with an assessment of the frequency of intra- and postoperative complications.Materials and methods. In the Clinic of Ear, Throat and Nose Diseases of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia (Sechenov University) a number of experimental and clinical studies were conducted, partial results of which we decided to retrospectively compare and analyze. The data of the first group of patients were taken from the results of a study with a CO2 laser. The data of the second group of patients were obtained from the results of a study with a diode (blue) laser with a wavelength of 445 nanometers. The data of the third group of patients were collected from archival data of medical histories from 2020 to 2022, who used a set of micro-tools for stapedoplasty (manual micropeforator, microneedle).Results. The data obtained showed similar results in the CO2 and blue laser groups. The main difference in the group of patients who underwent stapedoplasty with classical microinstruments was a longer operation time compared to laser stapedotomies, as well as a greater number of intraoperative difficulties associated with the mobilization of the foot plate of the stapes during manipulations with a manual perforator.Conclusions. The use of blue laser and CO2 laser in stapedotomy procedures shows promising results in terms of surgical accuracy and speed of the operation. Further research should compare the long-term effects of these three methods to determine the most effective and safest.

Impact of Coherent Laser Irradiation on Germination and Mycoflora of Soybean Seeds—Innovative and Prospective Seed Quality Management

Laser irradiation is considered a new technology in agriculture; however, the success of irradiation depends on the selection of precise parameters for the light source and exposure. In this study, the impact of laser stimulation on germination and the occurrence of mycoflora in soybean seeds was assessed. The following factors were considered: (1) irradiation using blue and red coherent lights, (2) irradiation of seeds only (a), use of Bradyrhzobium japonicum vaccine only (b), and irradiation of the seeds plus the Bradyrhzobium japonicum vaccine (c). The germination index, seedling weight and seeds infected by fungus were determined. It was found that the laser treatment of seeds increased germination and seedling weight. Laser irradiation affected the abundance of species Phoma glomerata, Botrytis cinerea, Rhizopus nigricans and Gliocladium roseum. The use of blue laser (LB—514 nm) reduced the number of the non-pathogenic species, R. nigricans and G. roseum.

Characterization of dough baked via blue laser

Depth of heat penetration and temperature must be precisely controlled to optimize nutritional value, appearance, and taste of food products. These objectives can be achieved with the use of a high-resolution blue diode laser—which operates at 445 nm—by adjusting the water content of the dough and the exposure pattern of the laser. Using our laser, we successfully cooked a 1 mm thick dough sample with a 5 mm diameter ring-shaped cooking pattern, 120 repetitions, 4000 mm min−1 speed, and 2 W laser power. Heat penetration in dough products with a blue laser is significantly higher compared to with an infrared laser. The use of a blue laser coupled with an infrared laser yields most optimal cooking conditions for food layered manufacture.

Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.

The purpose of this study was to analyze the influence of blue laser on bacterial growth of the main species that usually colonize cutaneous ulcers, as well as its effect over time following irradiation. The use of blue laser has been described as an adjuvant therapeutic method to inhibit bacterial growth, but there is no consensus about the best parameters to be used. Strains of Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, and Escherichia coli ATCC 25922 were suspended in saline solution at a concentration of 1.5×10(3) colony forming units (CFU)/mL. Next, 300 μL of this suspension was transferred to a microtitulation plate and exposed to a single blue laser irradiation (450 nm) at fluences of 0 (control), 3, 6, 12, 18, and 24 J/cm(2). Each suspension was spread over the surface of a Petri plate before being incubated at 37°C, and counts of CFU were determined after 24 and 48 h. Blue laser inhibited the growth of S. aureus and P. aeruginosa at fluences >6 J/cm(2). On the other hand, E. coli was inhibited at all fluences tested, except at 24 J/cm(2). Blue laser light was capable of inhibiting bacterial growth at low fluences over time, thus presenting no time-dependent effect.