Use Of Alternative Products Research Articles

Neonicotinoid insecticides in a large-scale agricultural basin system-Use, emission, transportation, and their contributions to the ecological risks in the Pearl River Basin, China

Neonicotinoid pollution has increased rapidly and globally in recent years, posing significant risks to agricultural areas. Quantifying use and emission, transport and fate of these contaminants, and risks is critical for proper management of neonicotinoids in river basin. This study elucidates use and emissions of neonicotinoid pesticides in a typical large-scale agriculture basin of China, the Pearl River Basin, as well as the resulting agricultural non-point source pollution and related ecological risks using market surveys, data analysis, and the Soil and Water Assessment Tool. Neonicotinoid use in the basin was estimated at 1361 t in 2019, of which 83.1 % was used in agriculture. After application, approximately 99.1 t neonicotinoids were transported to the Pearl River, accounting for 7.2 % of the total applied. Estimated aquatic concentrations of neonicotinoids showed three seasonal peaks. Several distinct groups of neonicotinoid chemicals can be observed in the Pearl River, as estimated by the model. An estimated 3.9 % of the neonicotinoids used were transported to the South China Sea. Based on the present risk assessment result, several neonicotinoids posed risks to aquatic organism. Therefore, the use of alternative products and/or reduced use is deemed necessary. This study provides novel insights into the fate and ecological risks of neonicotinoid insecticides in large-scale watersheds, and underscores the need for greater efficiency of use and extensive environmental monitoring.

Incidence of fungal infections in melon fruits treated with alternative products

There is an increased demand for alternative products instead of synthetic fungicides for the management of postharvest diseases in melon crops to prevent the development of quiescent infections that cause great economic losses. In the present study, we evaluated the cantaloupe and orange flesh melon cultivars after treatment with acibenzolar-S-methyl, methyl jasmonate, Agro-Mos®, calcium phosphite and potassium phosphite. Treatments with methyl jasmonate, potassium phosphite and Agro-Mos® reduced the incidence of fungal infections in the treated fruits. The use of alternative products should extend the management strategies to the pre-harvest period, with regard to enhancing the beneficial effects of these products and ensuring that they effectively target postharvest infections.

Commercial biostimulants had minimal effects on greenhouse grown blueberry vegetative growth

The drive to adopt sustainable practices in agriculture has led to the use of alternative products that either supplement and/or replace commonly used inputs. A nine-month greenhouse study was conducted to assess the effect of eight commonly used biostimulants on plant growth among four blueberry cultivars (‘Brightwell’, ‘Farthing’, ‘Legacy’ and ‘Premier’). The biostimulants did not show an effect on plant growth (i.e. height, width, volume, cane number, cane diameter, leaf area index) or plant biomass (dry weight). Similarly, no difference was detected between treated and untreated plants for the leaf pigment indicators (chlorophyll, anthocyanin, flavonols and NBI), with few exceptions. Terra Grow resulted in higher chlorophyll content than untreated plants in the cultivar Brightwell in February. Chlorophyll content was higher in the untreated plants than the biostimulant Fertiactyl GZ in ‘Legacy’ in May. Similarly, there were differences in anthocyanin and flavonols contents between the untreated plants and those treated with Terra Grow and Fertiactyl GZ in ‘Legacy’ and ‘Brightwell’ at two timepoints. However, the effects were not consistent over time or blueberry type. While biostimulants are often described as capable of providing benefits to plants in improving growth or alleviating biotic and abiotic stresses, such benefits were not evident with these products in a manner that was consistent in this study. Overall, the effects of the biostimulants considered in this study differed by blueberry cultivar and time and were not consistent.

PSI-23 Effect of Supplementation with Tannins and Saponins on Animal Performance of Holstein Calves

Abstract The optimization of the nutritional program of calves is essential for the sustainability and profitability of dairy companies. The use of additives such as Monensin allows to improve growth efficiency; however, concerns about the use of antibiotics in animal feed are growing. Therefore, the use of alternative products that can replace antimicrobial feed additives is necessary. Our objective was to evaluate the use of a combination of saponins and tannins in rearing stage of Holstein calves. A total of 36 female calves (BW, 114 ± 11 Kg; age, 86 ± 6 days) were distributed in 3 groups (n = 12/group) in a completely randomized design, using BW as criteria for assignment to each treatment (diet): 1) CON: without additives, 2) MON: Sodium Monensin (MON, 60 mg/kg DM, Elanco, Indianapolis, IN), 3) BXS: (900 mg/kg DM, a mixture of saponins and tannins, Silvafeed BXS, Silvateam, Argentina). The animal was used as the experimental unit. The calves were fed individually during an adaptation period of 10 days to the diet and a measurement period of 62 days. Body weight assessments were performed at the beginning and end of the measurement period with 12 previous hours of fasting. The feed intake was determined daily by measuring offer and rejection, and the conversion efficiency was determined using the quotient between the average daily gain and dry matter intake. Data were analyzed using PROC GLM of SAS, considering treatment as a fixed effect. Means differences were compared using Tukey's test, considering significance when P value ≤ 0.05. BXS and MON had increased final BW than CON (181, 181, and 174 kg respectively, P = 0.03). ADG was greater in BXS and MON than CON (1.075, 1.080, and 0.958 kg/d respectively, P = 0.03). BXS had similar DMI than MON but greater than CON (5.29, 5.13, and 4.62 Kg/d respectively, P = 0.03). The G:F was similar between treatments (0.208, P = 0.69). In conclusion, a combination of saponins and tannins can be used as an alternative additive to improve the performance of Holstein calves in rearing stage.

Utilización de macroalgas para la inducción de mecanismos defensivos ante agentes fitopatógenos causantes de enfermedades foliares en hortalizas

The production of vegetables worldwide has grown substantially, which has caused the demand for resources used to produce food to increase. Such is the case that nowadays, the use of pesticides for the production and management of different crops has become indispensable, causing a series of conflicts in nature, such as contamination of soil, water and air, making it a problem to be addressed. For this reason, different studies have been directed in the search for new alternatives for crop management, which involve the use of alternative products, one of them is algae extracts; these extracts have shown to have a wide variety of functions that contribute significantly to improve productivity without causing negative impacts on the environment. Among the benefits of using macroalgae extracts are the biostimulant activity, improvement of plant development, increase of water retention capacity, and activation of acquired resistance systems, among others, allowing the production of healthy food with less impact on the environment through the use of a renewable marine resource, which is currently available on the planet, becoming an essential productive alternative. Keywords: macroalgae extracts, effects, production, pathogens, elicitors, resistance systems.

Changes in the Use of Tobacco, Alternative Tobacco Products, and Tobacco Alternatives in Germany.

Due to the increasing use of alternative tobacco products (waterpipes, heat-not-burn) and tobacco alternatives (e-cigarettes), we studied recent changes in the prevalence of conventional tobacco use and alternative products. Data come from ten waves of the Epidemiological Survey of Substance Abuse (ESA) from 1995 to 2021, with representative samples collected via paperpencil questionnaires, telephone interviews, or online. We compared the prevalence of conventional tobacco use and alternative products by gender and age (18-24, 25-39, 40-59 years). In all age groups, the use of conventional tobacco products decreased. The prevalence of the exclusive use of one of the three alternative products differed statistically significantly between age groups and in 2021 was higher for ages 18-24 (females: 11.1%, males: 12.4%) compared to ages 25-39 (females: 2.9%; males: 4.9%) and ages 40-59 (females: 1.4%; males: 2.0%). The use of alternative products was mainly due to the exclusive use of waterpipes among individuals aged 18 to 24 and 25 to 39 and the exclusive use of e-cigarettes among individuals aged 40 to 59. The higher prevalence of alternative product use among young adults implies a turning point that needs to be considered in prevention. Because of the addictive potential of these products, young adults can be expected to maintain their use into middle and older adulthood. There is a need to monitor the use of alternative products, identify the risks associated with them, and develop effective prevention and cessation interventions for adults.

English

Amongst the most important agricultural products in Brazil, the soybean stands out, mainly as an export good. One of the end-of-cycle diseases, Anthracnose, caused by the fungus Colletotrichum truncatum (Schwein.) leads to severe damage to grain quality and yield. The use of natural products as a substitute for conventional pesticides for plant diseases control is an alternative found to reduce food contamination and environmental impacts. However, despite the progress made, much work still needs to be done to enable the safe and effective use of alternative products. Thus, this study aimed to test efficacy of noni essential oil (EO), combined with adjuvants, in the control of anthracnose in soybean plants. Gas chromatography was performed to identify the chemical profile of the EO. In vitro mycelial growth inhibition tests and analyzes on the stimulation of phytoalexin production were also performed. In the field trial, two soybean cultivars submitted to EO were used combined with orange and mineral oils as adjuvants. Octanoic acid (OA) was identified as the major component of EO. There was efficacy in the control of C. truncatum by EO and OA in vitro, as well as the induction of the production of the phytoalexin gliceolin in soybean seedlings with an increase of almost 80% with OA but with no impact on the growth of Trichoderma sp. Between the soybean cultivars tested, Coodetec 2728 IPRO presented lower severity to anthracnose symptoms, however, the cultivar Brasmax Foco demonstrated good tolerance to pathogen’s attack maintaining high productivity. Application of fungicides on the cultivar Coodetec 2728 IPRO resulted in a detrimental effect on yield possibly due to induction of the green stem syndrome formation in plants and a harvest delay of 15 days. Key words: Morinda citrifolia L., Glycine max L.; Colletotrichum truncatum, Trichoderma sp, alternative control.

Cyanobacteria: A Natural Source for Controlling Agricultural Plant Diseases Caused by Fungi and Oomycetes and Improving Plant Growth

Cyanobacteria, also called blue-green algae, are a group of prokaryotic microorganisms largely distributed in both terrestrial and aquatic environments. They produce a wide range of bioactive compounds that are mostly used in cosmetics, animal feed and human food, nutraceutical and pharmaceutical industries, and the production of biofuels. Nowadays, the research concerning the use of cyanobacteria in agriculture has pointed out their potential as biofertilizers and as a source of bioactive compounds, such as phycobiliproteins, for plant pathogen control and as inducers of plant systemic resistance. The use of alternative products in place of synthetic ones for plant disease control is also encouraged by European Directive 2009/128/EC. The present up-to-date review gives an overall view of the recent results on the use of cyanobacteria for both their bioprotective effect against fungal and oomycete phytopathogens and their plant biostimulant properties. We highlight the need for considering several factors for a proper and sustainable management of agricultural crops, ranging from the mechanisms by which cyanobacteria reduce plant diseases and modulate plant resistance to the enhancement of plant growth.

Efficiency of essential oils to control Colletotrichum theobromicola in vitro

Essential oils promote the inhibitory control of several fungi, including those within the genus Colletotrichum, the causal agent of Anthracnose, a disease which may occur at any stage of development in various crops, reducing up to 70% of crop production in some cases. Thus, the use of alternative products constitutes an important strategy for the integrated management, promoting less persistent molecules in the environment and lower toxicity rates, providing health benefits to producers and consumers of agricultural products. In this context, the present study evaluated the in vitro fungitoxic effect of essential oils from Java citronella (Cymbopogon winterianus), clove (Syzygium aromaticum), eucalyptus (Eucalyptus globulus) and rose pepper (Schinus terebinthifolius) on the mycelial growth of Colletotrichum theobromicola. The experiment was performed under completely randomized design, in a 4x5 factorial scheme (4 essential oils x 5 concentrations), with five replications, and the experimental unit consisting of a Petri dish. The treatments were generated by combining the concentrations (0, 5, 10, 15, 25 and 50 μL mL-1) of essential oils (citronella, clove, eucalyptus and rose pepper). The plates were inoculated with the pathogen C. theobromicola and incubated for seven days at 25 ± 2 °C. To verify the difference between treatments, the percentage of mycelial growth inhibition (PGI) was estimated. The mycelial growth of C. theobromicola was significantly reduced with increasing concentrations of essential oils. At a concentration of 50 μL mL-1, the essential oil from S. terebinthifolius showed the best result inhibiting 54.57% of mycelial growth, followed by the oil from S. aromaticum (49.26%), C. winterianus (23.70%) and E. globulus (17.90%). All the studied oils showed antifungal activity.

The Investigation of Mechanical Properties of Polypropylene Fiber-Reinforced Composites Produced With the Use of Alternative Wastes

Various studies on the disposal or storage of the wastes generated due to mining activities have been carried out until today. With the developing technology, the use of alternative products instead of aggregates in Composite and the effect of fiber addition were investigated. The most common of these studies is the bending strength tests on the beam sample. In this study, solid wastes generated by mining operations were used instead of aggregate used in Composite. Beam samples were poured with the dimensions of 40×40×160 mm by adding 4-6 kg/m3 polypropylene fiber to the mixture obtained by using these solid wastes; then, its effects on ultrasonic pulse velocity test, tensile test, and compressive strength tests were conducted. According to the results of these tests, it was found that the fibers had a positive effect on compressive strength and bending strength. Also, it was found that water absorption rates and densities did not have an effect, while the ultrasonic pulse velocity decreased with increasing fiber content.

Total phenolic content, antiradical, antimicrobial and antibiofilm properties of grape and apple vinegar

Antimicrobial resistance – the capability of microorganisms to resist antimicrobial agents – has been stated as a major concern for public health. The increase in spread of multi- and pan-resistant bacteria which are not treatable with present antimicrobials has brought the need for the use of alternative products. Vinegar is a natural product- produced from alcoholic fermentation- that has shown strong antimicrobial activity. The aim of this study was to determine the total phenolic content and antiradical activity of the commercial grape and apple vinegar as well as to evaluate their antibiofilm and antimicrobial activities against Staphylococcus aureus and Pseudomonas aeruginosa. Grape vinegar showed higher total acidity and total phenolic content, and lower antiradical activity (DPPH activity) compared to apple vinegar. The populations of S. aureus and P. aeruginosa were significantly reduced by neat grape and apple vinegar samples. The antibacterial activity of grape vinegar was superior to apple vinegar. While AV and GV samples at 50% concentration did not form a visible zone of inhibition against S. aureus, they showed an inhibitory effect against P. aeruginosa (16.24 mm for GV and 16.5 for AV). The vinegar applied at the lowest concentration (25%) did not show any antibacterial effect on either bacterium. Solutions containing 50% to 6.25% vinegar samples prevented almost 100% biofilm formation in both bacteria. However, solutions containing lower amounts of vinegar showed stronger inhibition of biofilm formation by P. aeruginosa. A positive correlation was found between the biofilm reducing ability of vinegar samples and their antibacterial activity in this study. Taken together, commercial grape and apple vinegar significantly reduced the viability of S. aureus and P. aeruginosa, thereby decreasing biofilm formation.

Botanical and chemical fungicides in the treatment of commercial seeds of Brachiaria brizantha and Panicum maximum

Brazil is the largest producer, consumer, and exporter of forage seeds in the world. However, the high incidence of pathogens threatens the seed production system, resulting in decreases in production and quality. Currently, the emphasis has been placed on the use of alternative products as a way of maintaining productivity and environmental sustainability. Thus, the objective of this study was to evaluate the mycoflora associated with the commercial seeds of Brachiaria brizantha and Panicum maximum treated with chemical and alternative fungicide. To test with chemical and alternative treatments, seeds were subjected to fluazinam + thiophanate-methyl, fludioxonil + metalaxyl-M, noni essential oil, rosemary pepper essential oil, sulfuric acid. There was also an untreated control. The most efficient products in the control of incident fungi were, in decreasing order, fluazinam + thiophanate-methyl, fludioxonil + metalaxyl-M, noni essential oil, rosemary pepper essential oil, and sulfuric acid. Fungicides mainly controlled the genera Alternaria, Aspergillus, Bipolaris, Chaetomium, Cladosporium, Colletotrichum, Curvularia, Fusarium, Penicillium, Phoma and Rhizopus. Essential oils showed positive effects in controllingBipolaris, Curvularia, and Fusarium. Sulfuric acid reduced the incidence of Bipolaris, Colletotrichum, Curvularia, Fusarium, Phoma, and Rhizopus. Seed treatment is one of the promising strategies for obtaining seeds of a healthy quality. As a result, the fungicides and botanical components listed above were promising in reducing fungi associated with forage seeds and can be used for seed treatments and in the development of new products with a natural base through essential oils.

South African Society of Anaesthesiologists Perioperative Patient Blood Management Guidelines 2020

Anaesthesiologists regularly request and administer blood components to their patients, a potentially life-saving intervention. All anaesthesiologists must be familiar with the indications and appropriate use of blood and blood components and their alternatives, but close liaison with haematologists and their local haematology blood sciences laboratory is encouraged. In the last decade, there have been considerable changes in approaches to optimal use of blood components, together with the use of alternative products, with a need to update previous guidelines and adapt them for anaesthesiologists working throughout the hospital system.

Nickel Allergy: Epidemiology, Pathomechanism, Clinical Patterns, Treatment and Prevention Programs.

Nickel is the most common cause of contact allergy in the general population and the most frequently detected allergen in patients patch tested for suspected allergic contact dermatitis (ACD). ACD from nickel is a typical type IV hypersensitivity. Nickel allergy is mostly caused by nonoccupational exposure, such as jewelry and clothing decorations, metal tools, medical devices (mainly orthopedic and orthodontic implants, cardiovascular prosthesis), eyeglasses, utensils, keys, pigment for paint, cosmetics, and food (mainly legumes, chocolate, salmon, peanuts). Occupational exposure can involve several workers (mechanics, metalworkers, platers, hairdressers, jewelers, workers in the constructions and electronic industries), classically involving hands and forearms. The classic clinical pattern of ACD caused by nickel is characterized by eczematous dermatitis involving the sites of direct contact with the metal. Non-eczematous-patterns are reported, including lichenoid dermatitis, granuloma annulare, vitiligo-like lesions, dyshidrosiform dermatitis, and vasculitis. In the case of systemic exposure to nickel, sensitized patients could develop systemic contact dermatitis. Patch testing represents the gold standard for the diagnosis of ACD from nickel. Treatment includes avoidance of contact with products containing nickel and the patient's education about the possible use of alternative products. A recent EU nickel directive, regulating the content and release of nickel from products, has caused a decrease of nickel contact allergy in some European countries. Nickel allergy is a relevant issue of public health with significant personal, social, and economic impact. This review summarizes epidemiology, pathomechanism, clinical patterns, treatment, and prevention programs.

The posthatch prophylactic use of ceftiofur affects the cecal microbiota similar to the dietary sanguinarine supplementation in broilers

The prophylactic administration of ceftiofur to newly hatched chicks is a common practice in some hatcheries worldwide to mitigate early gastrointestinal infections caused by Enterobacteriaceae. In spite of the crucial role of the gut microbiome for the broiler's health, there is still limited information on how the microbial composition is affected by such procedure. We investigated the effects of posthatch prophylactic application of ceftiofur on the cecal microbiota of 14-day-old broilers fed regular or sanguinarine-supplemented diets. DNA samples were extracted from cecal contents, amplified for the V3-V4 regions of the microbial 16S rRNA gene, and sequenced in a high-throughput sequencing platform (Illumina MiSeq). After downstream bioinformatics and statistical analyses, our results demonstrated that both ceftiofur and sanguinarine treatments similarly increased the proportions of the phylum Bacteroidetes and the genera Bacteroides and Megamonas, whereas reduced the relative abundances of Firmicutes and Lachnospiraceae in the ceca of the birds. Such changes are probably associated with increased carbohydrate fermentation processes favoring the production of short-chain fatty acids. This was also corroborated by the functional prediction findings, which suggest an increase in some metabolic pathways associated with digestibility in broilers receiving ceftiofur. Considering that antimicrobial stewardship in animal production systems is strongly needed to mitigate the threat of antimicrobial resistance, our findings show that supplementation with a phytogenic feed additive can lead to a similar microbial composition in the ceca of commercial broiler chickens, suggesting that the use of alternative products could lead to functional modifications without increasing pressure for antimicrobial resistance.

Safety of the Polish BCG-10 Vaccine During a Period of BCG Vaccine Shortage: An Australian Experience.

Bacille Calmete-Guerin vaccine is widely administered to reduce the risk of severe tuberculosis disease in children. Recent global vaccine supply issues have led to the use of alternative products, which may vary in side effect profile. We report on the safety of the Polish (Moreau strain) "Bacille Calmete-Guerin-10" vaccine in an Australian cohort. Using active surveillance, we identified an adverse event rate of 54.6 per 10,000 doses (95% confidence interval: 38.5-75.2), which was comparable to that reported with the Danish Sanofi-Pasteur and Connaught strains.

Essential Oils as Potential Alternative Biocontrol Products against Plant Pathogens and Weeds: A Review.

Naturally produced by aromatic plants, essential oils (EO) contain a wide range of volatile molecules, including mostly secondary metabolites, which possess several biological activities. Essential oils properties such as antioxidant, antimicrobial and anti-inflammatory activities are known for a long time and hence widely used in traditional medicines, cosmetics and food industries. However, despite their effects against many phytopathogenic fungi, oomycetes and bacteria as well as weeds, their use in agriculture remains surprisingly scarce. The purpose of the present review is to gather and discuss up-to-date biological activities of EO against weeds, plant pathogenic fungi, oomycetes and bacteria, reported in the scientific literature. Innovative methods, potentially valuable to improve the efficiency and reliability of EO, have been investigated. In particular, their use towards a more sustainable agriculture has been discussed, aiming at encouraging the use of alternative products to substitute synthetic pesticides to control weeds and plant diseases, without significantly affecting crop yields. An overview of the market and the recent advances on the regulation of these products as well as future challenges to promote their development and wider use in disease management programs is described. Because of several recent reviews on EO insecticidal properties, this topic is not covered in the present review.

Mechanical properties and microstructure of soils treated with a vinyl-based copolymer

In soil stabilisation, the use of alternative products to lime or cement has been proposed. However, the effects of these additives, of various origins, on soil behaviour and stabilizing mechanisms are not well understood. Thus, the aim of this paper is to characterize the modification of the mechanical behaviour and microstructure of two soils after treatment with one of those product, a vinyl based copolymer. After treatment, both materials were compacted up to their maximum dry density. The mechanical behaviour of the materials as a function of the curing conditions, suction and water content was first determined. In a second step, the alteration of the microstructure by the treatment product was assessed. The results showed that the treatment permitted to significantly improve the strength of the tested soils. Moreover, the efficiency of the product increases when the soil is dried. However, the Young modulus is not significantly modified by the treatment. Microstructural reorganization after co-polymer addition has been detected by SEM technique for kaolin samples, whereas no modification have been observed for silt samples.

Effect of the environment and use of alternative products in the post-harvest of papaya

Abstract This study aimed to establish an alternative treatment for post-harvest storage of Papaya (Carica papaya L.), var “Tainung 1” and to control major diseases at this stage. Two storage place were evaluated (room temperature and cold room); Six treatments (Control, Samurai 40ml/20l water, Serenade® 40ml/20l water, Sanatizer EUPROOFF® 10ml/20l water, EUPROOFF® + Serenade® + Samurai, Serenade® + Samurai), five storage periods (0, 3, 6, 9 and 12 days) and three seasons (December, January and February). Fruit weight loss (FWL), color index, number and size of lesions were evaluated and used to calculate the disease incidence and average of disease incidence. The FWL and index color of fruits were more affected by monthly variation than the treatments, especially the Samurai one. The growth of disease incidence were slower under cold room. Among the treatments, the Samurai was the most efficient,but if mixed to others products their efficiency was not observed. These results suggest that the using of Samurai only in cold room conditions can be more effective in control of post-harvest diseases of papaya.

UNUSUAL METHOD TO RELIEF DENTAL PAIN

Toothache is the great complaint for dental care. Generally, the patient postpones around 7 days, awaiting remission of pain, using medications and/or alternative resources. Difficulties of public access to treatment, financial issues, fear, and dental anxiety are frequently cited. There are reports of the use of alternative products found in the domestic environment to solve them, such as alcohol, toothpaste, and some foods like garlic and cloves among others. A 41-year-old woman, V.S.F., presented to attendance with toothache for 5 days, and as pain increased, in addition to analgesics, she applied 90 g (1 tube) of toothpaste over the region. It had a white plaque in the posterior region of the right oral mucosa and mucobuccal fold in the region 43 to 47. Attention was given to toothache 44 and orientation of palliative care in relation of oral mucosa lesion caused by chemical burn until remission of the process. Toothache is the great complaint for dental care. Generally, the patient postpones around 7 days, awaiting remission of pain, using medications and/or alternative resources. Difficulties of public access to treatment, financial issues, fear, and dental anxiety are frequently cited. There are reports of the use of alternative products found in the domestic environment to solve them, such as alcohol, toothpaste, and some foods like garlic and cloves among others. A 41-year-old woman, V.S.F., presented to attendance with toothache for 5 days, and as pain increased, in addition to analgesics, she applied 90 g (1 tube) of toothpaste over the region. It had a white plaque in the posterior region of the right oral mucosa and mucobuccal fold in the region 43 to 47. Attention was given to toothache 44 and orientation of palliative care in relation of oral mucosa lesion caused by chemical burn until remission of the process.