Uruguay River Research Articles

Density of Limnoperna fortunei larval stages in a cascade of subtropical reservoirs: spatiotemporal variation and environmental influences.

The golden mussel (Limnoperna fortunei) is an invasive bivalve that has established itself in several South American river systems, impacting ecosystem functioning. Reservoir cascades provide their larvae with the means of rapid dispersal, but the relationship between environmental variables and larval stage structure remains unclear. In this study, the density of three L. fortunei larval stages and quantitative detection using DNA are analyzed in a cascade of five reservoirs in the upper Uruguay River Basin and associated with spatiotemporal variation in environmental parameters. The analysis of L. fortunei eDNA presence and absence in freshwater systems appears to be a valuable mapping tool; however, no significant link was found between the eDNA magnitude and the overall larval density. The increase in larval density was related to the fluctuation of environmental parameters over a year, with the highest average larval densities observed in the CN and ITA reservoirs, though no significant difference was observed between the five reservoirs, where D-shaped larvae predominated. During winter, larval density decreased significantly, however, other variables also contribute to species activity and development in the upper Uruguay River Basin reservoirs and may be considered limiting factors. The relationships between environmental parameters were evaluated using a multivariate model. The interaction between reservoir area and precipitation, water temperature, electrical conductivity, and dissolved oxygen had a significant effect on larval density but showed specific influences on each larval stage. Any increase in density was regulated by dissolved oxygen and electrical conductivity content at all larval stages. Furthermore, total phosphorus affected the density of F1 and F3 larvae. The interaction between reservoir area and precipitation, nitrate content, phosphate concentrations, and water temperature had the most influence on the density of F2 and F3 larval stages; the F1 stage was mainly affected by calcium concentrations. The isolated effect of precipitation also contributed to the density of F2 and F3 larvae. Our findings shed light on the interaction between different phases of golden mussel larvae and the main nutrients found in reservoirs, which may be a determining factor in the rise in density of the non-native species in these systems.

Pesticides in fish from the Uruguay River and markets in Argentina and health risk assessment

ABSTRACT Organochlorine, organophosphate, triazole, and strobilurin pesticides were determined in fish samples. Relative standard deviations lower than 9.3% were obtained for organochlorine pesticides and 10.8% for other pesticides. Accuracy ranged from 73% to 119% for organochlorine pesticides and 80.4% to 116% for organophosphate, triazole, and strobilurin pesticides. A total of 28 pesticides were analysed and 7 of them were detected (exceeding 10 µg/kg) in some samples, with the highest concentration recorded at 68.5 µg/kg, corresponding to heptachlor epoxide A. The pesticide most frequently detected was β HCH, found in 30 of the 100 analysed samples. Hazard Quotient values were estimated for men, women, and children. These values exceeded 1 for heptachlor epoxide in women and children, as well as for endrin in children. These findings emphasise the need for stricter controls to reduce fish contamination and mitigate health risks.

Sphaerotilus uruguayifluvii sp. nov., a novel filamentous bacterium isolated from river water.

Strain C29T, a Gram-staining-negative, straight rod occurring singly, in pairs or short chains, was isolated from floating filamentous biomass of the Uruguay River. The strain was catalase and oxidase positive, chemoorganotrophic, strictly aerobic, non-motile, and grew at pH 6.0-9.0, 15-45°C, and 0-0.5% (w/v) NaCl. Polyhydroxybutyrate was accumulated in nutrient-limited conditions. Phylogenetic analysis based on the 16S rRNA gene revealed that strain C29T had the highest sequence similarity with Leptothrix discophora SS-1T (97.82%), Ideonella livida TBM-1T (97.82%), Vitreoscilla filiformis L1401-2T (97.52%), Sphaerotilus sulfidivorans D-501T (97.50%) and Sphaerotilus natans DSM6575T(97.46%). Other type strains with validly published names had similarities below 97.46%. Further phylogenomic analysis showed that strain C29T was affiliated to the family Sphaerotilaceae. Average nucleotide identity (ANI) and in silico DNA-DNA hybridization (dDDH) values with its phylogenetic relatives were lower than 91 and 41%, respectively, revealing that strain C29T represented a new species. The DNA G + C content of strain C29T was 70.9%. The annotation of the genome of the novel strain shows it possessed genes for the degradation of aromatic compounds. It also contained genes that encode sigma factors involved in response regulation of stress resistance, which is an important function for adaptation and survival in natural niches. Based on the results of the phylogenetic and phenotypic analyses, we propose that strain C29T represents a novel species, for which the name Sphaerotilus uruguayifluvii sp. nov. is proposed. The type strain is C29T (= CCM 9043T = DSM 113250T).

Five new unexpected populations of endangered tuco-tuco Ctenomys rionegrensis (Rodentia, Ctenomyidae) help understanding its distribution and historical biogeography

In this study, we conducted a survey of newly discovered populations of tuco-tuco (subterranean rodents of the genus Ctenomys) in the Entre Ríos province, in an area characterized by its unexplored nature and its climatic and biogeographic complexity within Argentina, which includes two National Parks. We characterize the nucleotide sequences of the cytochrome-b gene, revealing the presence of seven novel haplotypes within Ctenomys rionegrensis, a species known to inhabit both sides of the Uruguay River. Through Bayesian analyses, we estimated the divergence times of the oldest lineages of C. rionegrensis, as well as those of the haplotypes located east of the Uruguay River, dating back approximately 630,000 years before present (ybp) and 526,000 ybp, respectively. These estimates correspond with significant paleogeographic events in the region. Our findings may raise questions regarding the taxonomic classification of the species and suggest potential modifications to its current endangered status as designated by the International Union for Conservation of Nature (IUCN). Consequently, this research provides valuable insights that may inform future revisions of the species' conservation status and guide the development of informed management strategies/policies.

Precipitation Extremes and Trends over the Uruguay River Basin in Southern South America

This study analyzes the spatial distribution and trends in five extreme daily rainfall indices in the Uruguay River Basin (URB) from 1993 to 2022 using the Climate Hazards Group Infrared Precipitation with Stations (CHIRPS) dataset. The main findings reveal a predominantly positive trend in heavy precipitation (R95p) and extreme precipitation (R99p) events over the mid URB, while a negative trend is observed in the upper and low URB. Significant trends in the frequency of heavy and extreme rainfall were observed during autumn (MAM), with positive trends across most of the mid and upper URB and negative trends in the low URB. In the upper URB, negative trends in the frequency of extremes were also found during spring (SON) and summer (DJF). Overall, there was a reduction in the number of consecutive wet days (CWD), particularly significant in the upper URB and the northern half of the mid URB. Additionally, the upper URB experienced an overall increase in the duration of consecutive dry days (CDD).

Species boundaries of the whiptail catfish Rineloricaria (Siluriformes: Loricariidae) from the Paraíba do Sul River drainage, southeastern Brazil, with species redescriptions and description of a new species.

Species of the catfish genus Rineloricaria are common in the Paraíba do Sul River basin, in southeastern Brazil; here we present a revision of the taxonomic diversity and geographic distribution of the species of the genus inhabiting the basin, based on novel morphologic and molecular data. Five species delimitation methods based on cytochrome C oxidase subunit 1 nucleotide sequences yielded comparable molecular operational taxonomic units. The automatic barcode gap discovery, assemble species by automatic partitioning, barcode index number, and Bayesian implementation of the Poisson tree process methods supported the recognition of five evolutionary lineages. These taxonomic units were assigned to the previously described Rineloricaria nigricauda, Rineloricaria steindachneri, Rineloricaria zawadzkii, and Rineloricaria nudipectoris, and an additional undescribed species. R. zawadzkii was further divided into two intraspecific geographically structured lineages using the generalized mixed Yule coalescent delimitation method. A maximum likelihood phylogenetic analysis revealed that the five lineages from the Paraíba do Sul have closer relationships to different species from southern and southeastern Brazil (Ribeira de Iguape, Lagoa dos Patos, Uruguay, Paraguay, and Parana river basins) than to each other. Based on the analysis of lectotypes, recently collected material, and specimens from ichthyological collections, the poorly described R. nigricauda and R. steindachneri are redescribed following current descriptive standards. The undescribed species from the middle and upper Paraíba do Sul River basin is formally described. The description of a new species, along with the description of species boundaries in R. nigricauda and R. steindachneri, contributes to the knowledge of the ichthyofauna of the Paraíba do Sul River basin and adjacent coastal drainages of southeastern Brazil. An identification key for the species of Rineloricaria occurring in the Paraíba do Sul River basin is provided.

An invasive bivalve with the potential to reconstruct chronologies of geomarkers in a large South American basin.

The Sr/Ca and 87Sr/86Sr ratios are used as natural indicators (geomarkers) in fish to reveal migratory patterns, due to significant relationships between these ratios measured in the water and in the fish calcified structures (otoliths, bones). The aim of this study was to assess the potential use of the Limnoperna fortunei shell as a proxy for monitoring the spatial and temporal variability of Sr/Ca and 87Sr/86Sr in water. These ratios were compared in water samples and bivalve shells proceeding from eight sites of four hydrogeological regions of the La Plata Basin (Argentina and Uruguay), collected in two hydrological periods (winter and summer) in order to depict the pattern of geographical and temporal variability and to evaluate the relationship between both matrices. Significant positive linear relationships were found between shell edge and water for 87Sr/86Sr (R2 = 0.96-0.97, p < 0.005) and Sr/Ca (R2 = 0.83-0.86, p < 0.05) in summer and winter. Slopes did not differ significantly from 1 (p > 0.05) for 87Sr/86Sr, while they differed from 1 for Sr/Ca in both seasons (p < 0.05), indicating that the elemental ratio would be influenced by endogenous/exogenous factors. No significant seasonal differences were found for both natural tags (p > 0.05), except for the lower Uruguay River (87Sr/86Sr) and outer estuary (Sr/Ca). These results indicate that the L. fortunei shell could be a valuable tool for monitoring spatio-temporal variations in water 87Sr/86Sr. Shell Sr/Ca shows high potential utility as a geomarker in freshwater, but additional assessments are needed to understand the impact of temperature and growth on this ratio.

Longitudinal changes on ecological diversity of Neotropical fish along a 1700 km river gradient show declines induced by dams

In naturally flowing waters, communities are expected to increase their taxonomic and functional diversity as well as increase the complexity of food web architecture along the longitudinal gradient from headwaters to mouth. However, these theories do not necessarily apply to dammed rivers. We analysed fish communities at 12 locations along the 1700 km stretch of the transnational Uruguay River. We determined species richness, beta-diversity and its components (turnover and nestedness), trophic diversity, and isotopic niches at community level, with particular focus on areas upstream and downstream of the four existing dams. We estimated trophic diversity metrics based on diet analysis, and isotopic niche breadth based on Bayesian isotopic niche models. We detected consistent longitudinal changes, supporting predictions from the River Continuum Concept. However, taxonomic richness, trophic diversity, and isotopic niche decreased abruptly immediately downstream of dams, and the component contributing the most to beta diversity was turnover, with the highest values occurring mainly after the cascade of dams in the upper Uruguay River, fitting expectations from the Serial Discontinuity Concept. The dam-free stretches of the Uruguay River allowed the recovery of ecological diversity as fish richness and trophic and isotopic niche increased. The negative impacts of dams should urgently be considered in the Neotropics, where many large dams are projected.

First Assessment of a Pebble Tool Industry in the Pesqueiro River Valley, Upper Uruguay River Basin, Southern Brazil

Abstract This article presents the first findings of a topographic survey plotting the location of archaeological material and of a technological study of the lithic industry at the SC-CHA-030 open-air archaeological site along the Pesqueiro riverbank, located in the upper course of the Uruguay River Basin in southwestern Brazil. We analyzed raw material selection and the production of shaped/façonnage tools (unifacial, bifacial, and trihedral) associated with the production (debitage) of cortical and semi-cortical flakes that were then transformed into tools by simple retouch. From the geoarchaeological point of view, the spatial distribution pattern of the material is meaningful in the context of the geomorphic transition between foothills and alluvial plain. Our study of technological behavior and the formation of archaeological sites finds that raw material and shaped pebble tools are a crucial aspect of the industries on the Paraná Basaltic Plateau of southern Brazil.

New fossil woods (upper Pleistocene) from the lower-middle Uruguay river basin (South America) reveal the past distribution of Aspidosperma (Apocynaceae)

The present work describes the taxonomic and paleobiogeographic study of two fossil woods related to extant Aspidosperma. The silicified specimens come from the fossil localities of Santa Ana (30°54′S, 57°55′W) and Concordia (31°19′S, 57°59′W), Entre Ríos Province, Argentina, belonging to the El Palmar Formation (Late Pleistocene). This unit represents the sedimentary body of the upper fluvial terrace generated by the Uruguay River in its middle basin in eastern Argentina. The anatomical features that distinguish the woods are growth rings delimited by axial parenchyma and fibers, semi-ring to-diffuse-porous woods; mainly solitary vessels; simple perforation plates; alternate, bordered, and vestured intervessel pits; scarce paratracheal and diffuse apotracheal axial parenchyma; homocellular, and uniseriate to-triseriate rays; non-septate fibers. Climate reconstruction modelled at the regional scale (Ecological Niche Modeling) revealed variations in macroecological diversity patterns of the nearest living relatives (Aspidosperma australe and A. polyneuron) over the last ca. 130,000 years. Optically stimulated luminescence dating of sediments from the upper part of the El Palmar Formation in the type area reveals that the unit spans from the Last Interglacial period (warm substage, MIS marine isotope stage 5a), to the penultimate interglacial (MIS 7). This period was characterized by warmer and wetter conditions than those observed today. The eco-anatomical characteristics of the fossil record reflect this type of environment. The modern analogues of the fossils studied here are now part of the forests that integrate the Atlantic forest and Araucaria forest biogeographic provinces in South America.

The International Court of Justice: A Proper Forum for the Balanced Adjudication of Trade-Environment Disputes

The World Trade Organization’s (WTO) Dispute Settlement Body (DSB) sometimes adjudicates cases with environmental undertones while hearing trade disputes. Considering that the DSB is mainly responsible for the application of WTO international trade rules to these cases, it is arguable whether the DSB is the most appropriate adjudicatory forum on cases with environmental undertones. The article analyses four cases decided by the DSB: (1) The United States – Restrictions on Imports of Tuna (Tuna-Dolphin I), (2) the United States – Restrictions on Imports of Tuna (Tuna-Dolphin II), (3) the European Communities – Measures Affecting the Approval and Marketing of Biotech Products (Biotech Product’s case), and (4) the United States – Import Prohibition of Certain Shrimp and Shrimp Products (the US Shrimp case). It also analyses four cases with trade and environment considerations decided by the International Court of Justice (ICJ): (1) Whaling in the Antarctic (Australia v Japan), (2) Gabčíkovo-Nagymaros (Hungary v Slovakia), (3); Certain Activities carried out by Nicaragua in the Border Area (Costa Rica v Nicaragua)/Construction of a road in Costa Rica along the San Juan River (Nicaragua v Costa Rica); and (4) Pulp Mills on the River Uruguay (Argentina v Uruguay). From the analysis, this article finds that the ICJ, rather than the DSB, would be the appropriate arbiter of trade cases with environmental undertones. This article finds that, unlike the DSB, the ICJ has a history of balanced adjudication of cases with trade-environment conflict and appears a better fit to decide cases with elements of trade and environment. As such, this option would guarantee a more neutral avenue for the adjudication of trade-environment conflicts.

Geoarchaeology of open-air sites of the Foz do Chapecó area in the upper Uruguay river, southern Brazil

This paper presents the results of the investigations that defined the formation of the sedimentary deposits and the chronological sequence of the Foz do Chapecó archaeological area in the Volta Grande of the Upper Uruguay River, Southern Brazil. This research was undertaken to contribute to the definition of the diachronic, sedimentary and stratigraphic context of the archaeological occupations. To achieve these goals a geoarchaeological prospection by land and water was conducted, focusing on the valley bottoms, in the whole Volta Grande area. It comprehended the characterization of surface sedimentary deposits, the exposure and analysis of stratigraphic profiles, and sedimentological analysis. Four superficially exposed deposits were identified: i.e., fluvial conglomerates, fine sediments with sandy texture, clay colluviums and current and recent alluvial deposits. Six interesting points for the opening of stratigraphic profiles were identified, and subsequently resulted in the identification of sedimentary facies associated with river-bed, colluvial and colluvial-alluvial deposits. Three of these points presented a clear sequence of archaeological levels in the stratigraphic context. The results indicated that the local sediments are predominantly composed of a variable mixture of silt and clay, with sands occurring predominantly in an interdigitated form. The stratigraphic structure of the identified layers led to the establishment of 7 sedimentary facies, associated with colluvium (Cm), colluvium-alluvium (Sm) and alluvium and fluvial channels (Sm, Fl, Gms, Gt, Gp, Gh). The radiocarbon dating gives an age between 12.0 and 0.3 cal kBP, indicating that the filling of the local valley occurred during the end of the Pleistocene and throughout the Holocene by means of both fluvial and colluvial systems. Nine distinct archaeological levels, with a clear stratigraphic, chronological, and technological characterization were identified. The pre-ceramic archaeological sequence shows the following chronology: the oldest occupation occurred between 12.0 and 11.0 cal kBP (Cm facies); this was followed by an occupation between 10.5 and 9.5 cal kBP (Cm, Gt, Gp and Gh facies in three archaeological sites with eleven 14C data), then an occupation between 8.7 and 8.5 cal kBP (Cm facies), another between 5.7 and 5.5 cal kBP, one more between 4.8 and 4.6 cal kBP (Cm facies), and the last between 3.4 and 3.3 cal kBP (Cm and Sm facies). The ceramic phase seems to form a single dense component with the anthropogenic soil formation, and was dated between 0.5 and 0.3 cal kBP (Sm facies). These data present a previously unpublished chronostratigraphic sequence that is unique for the context of open-air sites in inner southern Brazil.

Altitudinal distribution and body condition of migratory fish juveniles Megaleporinus obtusidens (Valenciennes) and Salminus brasiliensis (Cuvier) in the main channel of the Uruguay River, Southern Brazil.

In this study, we analyzed the altitudinal distribution and body condition of the juvenile migratory fish Megaleporinus obtusidens and Salminus brasiliensis in the Uruguay River, a South American subtropical river. We used the presence/absence data and condition factor (K) of juveniles as indicators to try to characterize some recruitment patterns of migratory fish in the main channel of the Uruguay River. Gillnet sampling was conducted during two reproductive years, 2015-2016 and 2016-2017 (November and March of each reproductive year), in three Uruguay River stretches at different levels of altitude. The nets remained in the water for 10-12 hours per night or day for 10 days during each field trip (NOV-15, MAR-16, NOV-16, and MAR-17). The abundance of M. obtusidens juveniles were similar between the two reproductive years, whereas S. brasiliensis capture was higher during the 2015-2016 reproductive year. The probability of finding M. obtusidens juveniles in the Uruguay River was negatively correlated (p < 0.05) with the altitudinal gradient of the sampling points, as shown by the GLM model. For S. brasiliensis, the logit model indicated a non-significant probabilistic relationship (p > 0.05) between the presence of juveniles of this fish species and the altitude gradient. Regarding the body condition of juveniles, the water quality of the sampling points did not affect the juvenile condition factor for either species; however, a positive relationship (p < 0.05) was found with the water level for both species, even with an adjusted R2 of 0.13 for M. obtusidens and 0.48 to S. brasiliensis. Altitude gradient and water level were factors that may affect the juvenile distribution and body condition of these species, respectively, in the Uruguay River. Therefore, this information can be used in hydropower generation policies to conserve migratory fish populations in the Uruguay River Basin.

Characterization of the recent (2019–2022) La Plata Basin hydrological drought from a centennial-scale perspective

Several major rivers within the La Plata Basin (LPB), the third largest basin in the world, have experienced record-low water levels between 2019 and 2022, with significant impacts for the economy of the region. This hydrological drought originated from a precipitation deficit over the headwaters of the Paraná, Paraguay, and Uruguay rivers, in response to an unusual multi-year La Niña episode. The objective of this study is to characterize the hydrological drought and quantify its unusualness by analyzing a set of indices based on daily, monthly, and annual streamflow and water levels of the main rivers of LPB, using centennial records. The results indicate that the recent hydrological drought was unprecedented in the context of the past 50 years in terms of severity and duration, featuring extreme drought conditions and duration over 25 months. The atmospheric drivers of the drought are analyzed, and future perspectives for water management are discussed.

Environmental macroscopic analysis of shallow wells containing water for human consumption in rural properties in Brazil

Water is an essential natural resource for maintaining life and its quality is influenced by the land use and occupation within a watershed. Furthermore, water quality has a direct impact on the health and well-being of a population. The present study aimed to carry out a macroscopic environmental analysis in shallow wells found in rural properties distributed in the states of Rio Grande do Sul and Santa Catarina, including the Uruguay River Basin. The evaluation period of the macroscopic parameters took place in April 2021. The physical characteristics of the water and the state of preservation of the shallow wells and their surroundings were visually evaluated in rural properties with predominant land use and occupation through dairy production, swine farming activity and seasonal crops (agricultural crops). As results obtained for the environmental macroscopic analysis, through the weighting of the characteristics observed in each parameter about the state of preservation of the shallow wells, it was verified that they presented different classifications, ranging from the Very Good class to the Poor, being possible to identify the main sources that cause negative impacts on the evaluated locations (state of vegetation, use by animals, protection of the area and proximity to residences). Therefore, rural producers were advised to carry out protection and care measures around these wells that presented lower classifications, Fair (38,10%) and Poor (19,09%) to improve the conditions and aspects that directly influence the quality of water for human consumption.

Phylogenomics of the narrowly endemic Eurycheilichthys (Siluriformes: Loricariidae): Sympatric species with non-sister relationships suggest mainly allopatric speciation

Armored catfishes of the genus Eurycheilichthys are endemic to Southern Brazil and Misiones (Argentina) comprising nine species of small size, with a high degree of sympatry and species diversity distributed in two river basins. Here we use new genome-wide data to infer a species phylogeny and test species boundaries for this poorly known group. We estimate 1) the phylogenetic relationships of the species of Eurycheilichthys based on 29,350 loci in 65 individuals of nine species plus outgroups, and 2) the population structure and differentiation based on 43,712 loci and 62 individuals to estimate how geography may have acted on speciation and formation of the sympatric species groups. Analyses support the monophyly of the genus and suggest two species-inclusive clades (East and West) with high support and very recently diverged species. Western clade contains E. limulus (from upper Jacuí River basin) that is sister to Western species of the Taquari-Antas basin plus E. paucidens. The Eastern clade contains E. pantherinus (from Uruguay River basin) sister to the Eastern species of the Taquari-Antas basin E. coryphaenus, plus the central-distributed species E. planus and E. vacariensis, and the more widely-distributed species E. luisae. Eurycheilichthys luisae is not monophyletic and may contain one or more cryptic species or hybrid individuals. A stronger diversity on structure of lineages on the Taquari-Antas, when compared to upper Uruguay and Jacuí River basins, and the fact that most of the sympatrically distributed taxa have non-sister relationships suggest a scenario of mainly allopatric speciation and may indicate a more dynamic landscape with headwater capture events among these tributaries.

Molecular Characterization of Gastroenteric Viruses in Wastewater from Cities in Uruguay.

Group A Rotavirus, Human Astrovirus, and Norovirus (RVA, HAstV, and NoV) are recognized as the major causative agents of acute gastroenteritis in children and adults worldwide. The aim of this study was to determine the prevalence and molecular epidemiology of RVA, HAstV, and NoV in wastewater from three cities in Uruguay. Thirty-six samples from Bella Unión, Salto, and Fray Bentos cities were analyzed using quantitative and qualitative PCR. RVA was the most frequently detected virus (50%), followed by HAstV (39%), NoV GII (36%), and NoV GI (25%). RVA strains were characterized as P[8] and G3 based on the VP4 and VP7 genes, respectively. Among NoV-positive samples, genotypes GI.2, GI.3, GI.5, GI.6, GI.7, GII.2, GII.6, and GII.4 were detected, and only one HAstV genotype (MLB1) was found. Our wastewater-based epidemiological approach provides a snapshot of the overall genetic diversity of these viruses in three cities of the Uruguay River basin during 2017-2018. These findings reinforce the importance of this environmental surveillance tool for monitoring epidemiological trends of enteric viruses circulating in the population, which can be used to guide public health intervention.

Leaf morphology and anatomy of Schlechtendalia luzulifolia, a basal member of subfamily Barnadesioideae (Asteraceae)

Abstract Based on molecular phylogenetic studies, Barnadesioideae have been proposed to be the basal subfamily of Asteraceae. This is a complex of 10 genera and 87 species distributed primarily along the Andean mountains, Patagonia, and into southern Brazil and Uruguay. Phylogenetic analyses have recovered all genera as monophyletic groups and have provided insights to their inter-relationships. Four generic clades have been substantiated: (1) Chuquiraga, Doniophyton, and Duseniella; (2) Dasyphyllum; (3) Barnadesia and Huarpea; and (4) Archidasyphyllum, Arnaldoa, and Fulcaldea. The remaining genus, the monospecific Schlechtendalia, has been an outlier in the subfamily, with some previous analyses recovering it as basal for the entire subfamily, and others showing it as sister to Barnadesia and Huarpea (with weak support) as well as to other genera. Recent massive sampling of loci has confirmed Schlechtendalia as the sister genus for the subfamily. Schlechtendalia luzulifolia has morphology atypical for Asteraceae. The capitula are loose aggregations of florets, and the leaves are long and strap shaped, more reminiscent of monocots. Morphological and anatomical investigations of the leaves reveal long, laminar blades with parallelodromous vascularization. The vesture is often with ‘barnadesioid trichomes’, especially towards the base of the plant, plus additional uniseriate trichomes consisting of 3 to many cells, newly reported for the subfamily. Some glandular trichomes with 2-4 short cells also occur. The transverse anatomy of the leaves reveals a single epidermal layer on both surfaces, which also contain the stomata (the leaf being amphistomatic). The mesophyll is undifferentiated; the vascular traces are surrounded by sclerenchyma that not only encircles the traces but also extends towards the epidermis and connects with it. The morphology and anatomy of the leaves of Schlechtendalia are divergent in comparison with other genera of the subfamily. Chuquiraga, Doniophyton, and Huarpea have leaf adaptations for survival in xeric habitats, such as dense pubescence, grey surfaces, and revolute margins. Schlechtendalia, in contrast, is adapted to a more mesic environment, especially near the Atlantic Ocean and along the Uruguay and La Plata rivers. The leaves are oriented upright, which correlates with undifferentiated mesophyll and stomata on both epidermal layers. The stem is an underground rhizome, an adaptation that permits survival during seasonal drought in the austral summer in Uruguay and adjacent regions. It is hypothesized that Schlechtendalia may have become adapted to more mesic environments in the Miocene prior to the rise of the Andes and development of the modern arid environments, into which many of the other genera of the subfamily subsequently radiated.

Representation of Hydrological Components under a Changing Climate—A Case Study of the Uruguay River Basin Using the New Version of the Soil and Water Assessment Tool Model (SWAT+)

SWAT+ is a revised version of the SWAT model that has the capability to route flow across landscape units in the catchment, which is expected to improve the spatial representation of processes in watersheds. We applied the SWAT+ model in the Uruguay River Basin, an international river basin in South America with a total surface area of 370,000 km2, in order to (1) assess the water balance components, (2) represent their spatial distribution, and (3) examine their changes over time. The catchment was divided into uplands and floodplains and a decision table rule was developed based on streamflow data. The SPOTPY Python library was linked to SWAT+ and used as a tool to perform sensitivity analyses and calibration. The model represented the fluctuations of discharge well, although there was a general tendency to underestimate peak flows. Blue (precipitation and runoff) and green (evapotranspiration and soil water content) hydrological components were spatially plotted. Overall, SWAT+ simulated a realistic spatial distribution of the water cycle components. A seasonal Mann–Kendall test suggests a positive increasing trend in the average temperature (p-value = 0.007; Sen’s slope = 0.09), the soil water content (p-value = 0.02; Sen’s slope = 1.29), and evapotranspiration (p-value: 0.03; Sen’s slope = 1.97), indicating that the ecosystem experienced a changing climate during the simulation period. The findings presented in this study are of significant value for the impacts of sustainable management and the evaluation of climate change on water resources in the Uruguay River Basin.

Ursidae (Mammalia, Carnivora) from the Quaternary of western Rio Grande do Sul, Brazil: Taxonomical, stratigraphic, and chronological aspects

All known Pleistocene South American fossil ursids belong to Arctotherium, an extinct genus of tremarctine bears. Its occurrence encompasses many countries in Central and South America, with findings mainly from Argentina. In Brazil, the genus is recorded in eight states, highlighting Rio Grande do Sul due to its provenance from fluvial deposits. In contrast, all other Brazilian records come from caves or fossil tanks. The Arctotherium material from Rio Grande do Sul comes from the Passo do Juquiry site, located on the banks of the Quaraí River, which marks the frontier between Brazil and Uruguay. The fossils are MCN-PV 1940/A-K, a set of postcranial bones: first to sixth lumbar vertebrae, sacrum, left scapula, left humerus, right portion of the pelvic girdle, and left femur. The anatomic description of these bones confirmed their belonging to Arctotherium sp. due to their size, the form of the vertebral processes, the presence of the entepicondylar foramen of the humerus, and the morphology of the trochlea and capitulum. Body mass estimates resulted in values mostly above 400 kg. The first absolute age from Passo do Juquiry indicated 40,000 years BP. The stratigraphic framework of the outcrop conformed to a floodplain fines facies association, a succession of mudstones with carbonate nodules on top. This facies association has lithological correlations to the Brazilian Touro Passo Formation, the Uruguayan Sopas Formation, and the Argentinean Toropí/Yupoí Formation. While having similar features, the ages of the lithologically correlated facies associations do not always line up, indicating that the evolution of the Uruguay River basin throughout the Late Pleistocene followed a pattern, but not synchronously. The presence of carbonate levels suggests that the climate during the time of deposition was drier than the present one, with the carbonate-less middle layer representing a more humid period.