Urinary Shedding Of Leptospires Research Articles

Urinary shedding of leptospires in palearctic bats.

Leptospirosis is a bacterial zoonotic infection of worldwide occurrence. Bats, like other mammalian reservoirs, may be long-term carriers that maintain endemicity of infection and shed viable leptospires in urine. Direct and/or indirect contact with these Leptospira shedders is the main risk factor as regards public health concern. However, knowledge about bat leptospirosis in the Palearctic Region, and in Europe in particular, is poor. We collected urine from 176 specimens of 11 bat species in the Czech Republic, Poland, Republic of Armenia and the Altai Region of Russia between 2014 and 2019. We extracted DNA from the urine samples to detect Leptospira spp. shedders using PCR amplification of the 16S rRNA and LipL32 genes. Four bat species (Barbastella barbastellus n=1, Myotis bechsteinii n=1, Myotis myotis n=24 and Myotis nattereri n=1) tested positive for Leptospira spp., with detected amplicons showing 100% genetic identity with pathogenic Leptospira interrogans. The site- and species-specific prevalence range was 0%-24.1% and 0%-20%, respectively. All bats sampled in the Republic of Armenia and Russia were negative. Given the circulation of pathogenic leptospires in strictly protected Palearctic bat species and their populations, non-invasive and non-lethal sampling of urine for molecular Leptospira spp. detection is recommended as a suitable surveillance and monitoring strategy. Moreover, our results should raise awareness of this potential disease risk among health professionals, veterinarians, chiropterologists and wildlife rescue workers handling bats, as well as speleologists and persons cleaning premises following bat infestation.

Leptospirosis in cats: Current literature review to guide diagnosis and management.

Leptospirosis is the most widespread zoonosis worldwide. Mammals (eg, rats, horses, cows, pigs, dogs, cats and aquatic species, such as sea lions and northern elephant seals) can all be infected by leptospires. Infection in animals occurs through contact with urine or water contaminated with the bacteria. In people, the disease is acquired mainly from animal sources or through recreational activities in contaminated water. Literature on the clinical presentation of leptospirosis in cats is scarce, although it has been demonstrated that cats are susceptible to infection and are capable of developing antibodies. The prevalence of antileptospiral antibodies in cats varies from 4% to 33.3% depending on the geographical location. Urinary shedding of leptospires in naturally infected cats has been reported, with a prevalence of up to 68%. Infection in cats has been associated with the consumption of infected prey, especially rodents. Thus, outdoor cats have a higher risk of becoming infected. Clinical presentation of this disease in cats is rare and it is not known what role cats have in the transmission of leptospirosis. Ongoing work is needed to characterise feline leptospirosis. This review is aimed at all veterinarians, both general practitioners who deal with cats on a daily basis in private practice, as well as feline practitioners, since both groups face the challenge of diagnosing and treating infectious and zoonotic diseases. The current literature on leptospirosis in cats is reviewed. To date, few case reports have been published in the field, and information has mostly been extrapolated from infections in people and dogs. This review is expected to serve as a guide for the diagnosis and management of the disease in cats.

Screening for Leptospira DNA in blood and urine from 30 apparently healthy dogs

Summary Asymptomatic carriage of leptospires in dogs has not been extensively studied. A prospective study was conducted on thirty apparently healthy male dogs to screen for Leptospiral DNA in their blood and urine. All dogs were up to date with a bivalent vaccine against Leptospirosis. Microscopic agglutination test (MAT) and real-time PCR analysis in blood and urine were performed for each dog. Six of 30 (20%) dogs had positive PCR results: two from blood samples (all MAT titres ≤ 160) and four from urine samples (one with maximal MAT titres of 2560 and three with all MAT titres ≤ 160). Ten months later, PCR analyses of blood and urine were repeated for these six dogs. Four had negative PCR results from blood and urine. The other two, with positive urine results at the first sampling, had negative PCR results from urine and positive PCR results from blood. In this study, the urinary shedding of leptospires appeared to be transient. As shown previously, serological status was not correlated with PCR results. Low but detectable amounts of Leptospiral DNA in the blood of some apparently healthy dogs raises questions regarding the pathophysiology of Leptospirosis and the diagnostic consequences of high-sensitivity PCR assays.

Urinary shedding of leptospires and presence of Leptospira antibodies in healthy dogs from Upper Bavaria.

Leptospirosis is classified as a re-emerging zoonotic disease with global impor- tance. The aim of this study was to determine urinary shedding of leptospires in healthy dogs and to identify the shedded leptospire species. Furthermore, antibody presence against leptospires was evaluated. In a prospective study urine samples of 200 healthy dogs from Upper Bavaria were randomly collected and evaluated by real-time polymerase chain reaction (PCR) specific for the lipL32 gene of pathogenic Leptospira (L) spp. Positive samples were further character- ized via multilocus sequence typing (MLST) to identify the Leptospira species. Microagglutination test (MAT) was performed to determine serum antibody titers. Three of 200 urine samples were found to be PCR-positive resulting in a urinary shedding prevalence of 1.5% (95% confidence interval 0.3-4.5%). All three dogs had been vaccinated before with a bivalent vaccine, covering the serogroups Canicola and lcterohaemorrhagiae. One dog shed leptospires of the species L. borgpetersenii, and two of the species L. interrogans. Of all dogs, 17.0% had antibody titers ≥ 1:100, and 3.5% titers ≥ 1:400 to serovars of non-vaccinal sero- groups. Healthy dogs that shed leptospires represent a possible risk for humans and other animals. The study emphasizes the importance of general hygiene measures in veterinary practice while handling urine of all dogs, and the use of vaccines that protect against a broader range of serogroups and that prevent urinary shedding.

Antibiotic Efficacy in Eliminating Leptospiruria in California Sea Lions (Zalophus californianus) Stranding with Leptospirosis.

Stranded California sea lions (Zalophus californianus) along the California coast have been diagnosed with leptospirosis every year since at least the 1980s. Between September 2010 and November 2011, we followed 14 stranded California sea lions that survived to release and evaluated antibiotic efficacy in eliminating leptospiruria (urinary shedding of leptospires). Leptospiruria was assessed by real-time PCR of urine and urine culture, with persistence assessed using longitudinally collected samples. Serum chemistry was used to assess recovery of normal renal function. Microscopic agglutination testing (MAT) was performed to assess serum anti-Leptospira antibody titers, and the MAT reactivity patterns were consistent with L. interrogans serovar Pomona infection frequently observed in this population. Animals were initially treated for 6 to 16 d (median = 10.5; mean = 10.8) with antibiotics from the penicillin family, with some receiving additional antibiotics to treat other medical conditions. All urine cultures were negative; therefore, the presence of leptospiruria was assessed using PCR. Leptospiruria continued beyond the initial course of penicillin family antibiotics in 13 of the 14 sea lions, beyond the last antibiotic dose in 11 of the 14 sea lions, beyond recovery of renal function in 13 of the 14 sea lions, and persisted for at least 8 to 86 d (median = 45; mean = 46.8). Five animals were released with no negative urine PCR results detected; thus, their total shedding duration may have been longer. Cessation of leptospiruria was more likely in animals that received antibiotics for a greater duration, especially if coverage was uninterrupted. Real-time PCR results indicate that an antibiotic protocol commonly used to treat leptospirosis in rehabilitating California sea lions does not eliminate leptospiruria. It is possible that antibiotic protocols given for a longer duration and/or including other antibiotics may be effective in eliminating leptospiruria. These results may have important human and animal health implications, especially in rehabilitation facilities, as Leptospira transmission may occur through contact with animals with persistent leptospiruria.

Growth response and shedding of Leptospira spp. in urine following vaccination for leptospirosis in young farmed deer

AIM: To investigate the effect of vaccination against Leptospira serovars Hardjo-bovis and Pomona on growth rate and shedding of leptospires in urine in rising 1-year-old farmed red deer. METHODS: In early March 2007, 230 female and 205 male, 3-month-old deer on five farms were treated with streptomycin then were randomly allocated to a control group (n = 218), or were vaccinated (n = 217) with a bivalent whole-cell killed leptospiral vaccine (Leptavoid-2) followed by a booster 4 weeks later. These animals were isolated from other 3-month-old deer on each property until May, when all vaccinated and control deer were combined with deer not treated with streptomycin, for maximum exposure to natural leptospiral challenge. Seroconversion was monitored in control deer against Leptospira borgpetersenii serovar Hardjo-bovis and Leptospira interrogans serovar Pomona using the microscopic agglutination test (MAT). A cut-off of ≥ 1:48 was used to identify a seropositive response to infection, and ≥1:24 to indicate a response to vaccination. Urine was collected from females in all groups and tested for shedding of leptospires using bacterial culture and real-time PCR. Male deer from vaccinated and control groups were weighed every 2–3 months to measure growth rate. RESULTS: In vaccinated female deer, antibody was present to Hardjo-bovis in 39–73% and to Pomona in 78–100% of animals in May, with titres ranging between 1:24–1:96 and 1:24–1:1536, respectively; control female deer were all serologically negative. In November, female control deer were seropositive to Hardjo-bovis on 4/5 farms (seroprevalence range 4–78%) but seronegative to Pomona on all farms. Between March and November mean growth rate was higher in vaccinated than control deer (26 g/day) on one farm (p = 0.049) that had the highest prevalence of seroconversion (78–88%) and urinary shedding (56%). Between August and November on 4/5 farms, when almost all seroconversions occurred, growth rates were higher in vaccinated than control deer (31 g/day; p = 0.011). Urinary shedding of leptospires was detected by culture and/or PCR in 8/34 (24%) unvaccinated control and 20/38 (53%) animals not treated with streptomycin on 2/5 farms; no shedding was detected in vaccinated deer. The average risk difference for shedding between vaccinated and control deer was –11% (95%CI = –18 to –4) (p = 0.001). CONCLUSION: Vaccination against Leptospira serovars Hardjo-bovis and Pomona was associated with higher liveweight gain and reduced urinary shedding of leptospires in young deer naturally challenged by Hardjo-bovis. The association with liveweight gain was evident on some but not all farms.

Screening urinalysis in dogs with urinary shedding of leptospires

The aim of this study was to determine the utility of urinalysis and dipstick results in predicting leptospiruria in dogs. Ninety dogs, regardless of health status, were included in this study since January to August 2008. All the dogs went through a complete physical examination, and urine samples were collected by catheter or by cystocentesis. Polymerase chain reaction (PCR) testing was performed on urine samples to detect leptospiral DNA. Urinalysis was done at the same time. Of the 90 dogs included in the study, 28 (31%) were diagnosed as shedding leptospires in their urine. In terms of urinalysis test results, no significant difference was found between dogs with or without leptospiruria. Indeed, most of the dogs with positive PCR assay had normal urinalysis findings. The present study suggests that using urinalysis or dipstick is not recommended for screening the dogs that are actively shedding leptospires in their urine.