Phenolic pollutants represent a significant environmental concern due to their widespread presence in industrial effluents, agricultural runoff, and urban waste streams. Enzymatic bioremediation emerges as a promising approach for the efficient removal of phenolic pollutants from contaminated environments. Laccases exhibit remarkable catalytic capabilities in degrading phenolic compounds into less harmful or even inert products. In this study, magnetic cross-linked laccase aggregate was prepared using superparamagnetic metal-organic frameworks and then was applied for phenol removal. The obtained results showed that the magnetic cross-linked laccase aggregate retains its activity across a broad pH range. Interestingly, (NH4)2SO4 exhibited a positive influence on enzyme activity, resulting in a relative activity of 137.7 %. The findings suggest that an optimal concentration range of 0.4–0.8 % can achieve maximum enzyme activity while avoiding potential negative impacts associated with excessive cross-linking. Optimal pH and temperature conditions were identified for enhanced phenol removal efficiency as pH 4.5 and 45℃, respectively. The investigation revealed the remarkable reusability of the cross-linked enzyme over multiple cycles. Harnessing the power of enzymes for phenolic pollutant remediation holds immense promise for mitigating environmental pollution and safeguarding public health.
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