Bubbly flows appear in many heat and mass transfer equipment. The interfacial area concentration is a critical parameter in determining the performance of the heat and mass transfer systems. The one-dimensional one-group interfacial area transport equation (IATE) was developed based on area-averaged bubbly flow data collected under uniform inlet flow boundary conditions. The applicability of the IATE to the flow systems with non-uniform inlet flow boundary conditions was not comprehensively examined. This study investigated the effect of inlet liquid and gas velocity profiles on measured interfacial area concentration changes along the flow direction. The data used in this study were collected for vertical upward air-water two-phase flow in a rectangular channel with a gap length of 10 mm and width of 200 mm. The test conditions were the superficial gas velocity ranging from 0.0870 to 0.288 m/s and the superficial liquid velocity ranging from 0.515 to 2.53 m/s. The interfacial area concentrations with uniform and non-uniform inlet flow boundary conditions measured in a large vertical rectangular channel were compared under the same area-averaged flow rate conditions. Then, the predictive capability of the IATE to the bubbly flow test data with uniform and non-uniform inlet flow boundary conditions was assessed. Some non-uniform inlet flow boundary conditions, including channel-center peaking of liquid flow (CPF), channel-center peaking of gas flow (CPG), single-side-wall peaking of liquid flow (SPF), and single-side-wall peaking of gas flow (SPG), significantly affected the interfacial area concentration changes along the axial location at relatively high superficial gas or liquid velocity conditions. The IATE's predictive capability deteriorated for several non-uniform inlet flow boundary conditions, including double-side-wall peaking of gas flow (DPG) and single-side-wall peaking of liquid flow (SPF), specifically at relatively high superficial liquid velocity conditions. The maximum percentage error of the interfacial area concentration prediction by the IATE for these non-uniform inlet flow boundary conditions reached 75%.