An indoor simulation experiment was conducted to explore the effects of cuticular wax content and specific leaf area (SLA) on accumulation and distribution of PAHs in different tissues of wheat leaf. Three levels (0, 1.25, 6.0mgL-1) of mixed solution of five PAHs (Σ5PAHs) including phenanthrene (PHE), anthracene (ANT), pyrene (PYR), benz[a]anthracene (BaA), and benzo[a]pyrene (BaP) were sprayed on leaves of seven varieties of winter wheat for every other day during 20 consecutive days. Shoot and root biomass of wheat under 6.0mgL-1 Σ5PAHs exposure were 5.87 and 0.33g, which were significantly (p< 0.05) lower than those (7.14 and 0.65g) without spraying Σ5PAHs solution, respectively. Elevated Σ5PAHs concentration in spraying solution significantly (p< 0.0001) decreased cuticular wax content (59.1 and 65.1 vs. 67.8mgg-1) in leaves of wheat but exerted slight effects on SLA. Regardless of spraying Σ5PAHs or not, SLA in leaves of Jiaomai (269-276cm2g-1) and Zhengmai (265-285cm2g-1) and cuticular wax content (104-118mgg-1) in leaves of Zhengmai were significantly higher than other varieties of wheat, respectively. Σ5PAHs concentration in cuticular waxes ranged from 24,616 to 106,353μgkg-1, which was 2~3 orders and 1~2 orders of magnitude higher than that in mesophylls (46.0-535μgkg-1) and leaves (785-5366μgkg-1). There was a significant (r = 0.46, p < 0.05, n = 28) positive correlation between SLA and Σ5PAHs concentration in wheat leaves when spraying 1.25mgL-1 of Σ5PAHs. The present study indicated that cuticular wax content was significantly (p < 0.01) positive correlated with Σ5PAHs concentration in the leaves and the translocation factor (TFw-m) of PHE, ANT, PYR, and Σ5PAHs from cuticular wax to mesophyll. Based on principal component analysis (PCA), cuticular wax content was the main limiting factor for folia uptake of PAHs in winter wheat. The present study suggested that cuticular wax could play significant roles in foliar uptake of PAHs of wheat via affecting their accumulation in cuticular wax and translocation to mesophyll.