Upstart Bay Research Articles

Green Turtle (Chelonia mydas) Blood and Scute Trace Element Concentrations in the Northern Great Barrier Reef.

Marine turtles face numerous anthropogenic threats, including that of chemical contaminant exposure. The ecotoxicological impact of toxic metals is a global issue facing Chelonia mydas in coastal sites. Local investigation of C. mydas short-term blood metal profiles is an emerging field, while little research has been conducted on scute metal loads as potential indicators of long-term exposure. The aim of the present study was to investigate and describe C. mydas blood and scute metal profiles in coastal and offshore populations of the Great Barrier Reef. This was achieved by analyzing blood and scute material sampled from local C. mydas populations in five field sites, for a suite of ecologically relevant metals. By applying principal component analysis and comparing coastal sample data with those of reference intervals derived from the control site, insight was gleaned on local metal profiles of each population. Blood metal concentrations in turtles from coastal sites were typically elevated when compared with levels recorded in the offshore control population (Howick Island Group). Scute metal profiles were similar in Cockle Bay, Upstart Bay, and Edgecumbe Bay, all of which were distinct from that of Toolakea. Some elements were reported at similar concentrations in blood and scutes, but most were higher in scute samples, indicative of temporal accumulation. Coastal C. mydas populations may be at risk of toxic effects from metals such as Co, which was consistently found to be at concentrations magnitudes above region-specific reference intervals. Environ Toxicol Chem 2023;42:2375-2388. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Development and application of species-specific cell-based bioassays to assess toxicity in green sea turtles

Despite the detection of a wide range of contaminants in the blood of green turtle populations foraging in three locations of northern Queensland – Upstart Bay, Cleveland Bay and the Howick Group of Reefs, little is known about the effects of these contaminants on turtle health. Newly developed cell-based bioassays using green turtle primary cell cultures provide an ethical, reproducible, and high-throughput method for assessing the risk of chemical exposure sea turtles. In this project, the toxicity of six priority metals (Mn, Co, Mo, As, Sb, Cu) and blood extracts from foraging turtles were tested in two bioassays adapted to green turtle primary skin and liver cells. Cytotoxicity of metals and blood extracts was measured in primary skin fibroblast cells using a resazurin assay. Glutathione-S-transferase (GST) activity was measured in primary skin fibroblasts and primary liver epithelial cells following exposure to metals and blood extracts. Arsenic, molybdenum, cobalt and copper were found to be cytotoxic to green turtle skin cells. Only manganese, cobalt and copper were found to alter GST activity, predominantly in skin cells, indicating a higher sensitivity of green turtle skin cells compared to liver cells. Effect concentrations of metals in both bioassays were above concentrations found in turtle blood. Turtle blood extracts from the three foraging grounds showed differences in cytotoxicity and GST activity. In both assays, blood extracts of turtles from Upstart Bay were the most toxic, followed by those from Cleveland Bay, then the Howick Reefs, suggesting turtles from Upstart Bay and Cleveland Bay may be at risk from current concentrations of organic contaminants. This study demonstrates that species-specific cell-based bioassays can be used effectively to assess chemical risk in sea turtles and their foraging grounds, and could be applied to assess chemical risk in other marine wildlife.

Monitoring the health of green turtles in northern Queensland post catastrophic events

Between 2014 and 2017, the Rivers to Reef to Turtles (RRT) project examined the health of green turtles at two coastal sites impacted by urban and agricultural human activities (Cleveland and Upstart Bays) and one proposed pristine site (Howick Group of Reefs) in northern Queensland, Australia, through blood biochemistry and haematology, plasma protein electrophoresis, and clinical assessments including body condition and barnacle counts. Furthermore, cases of mortality were subjected to comprehensive postmortem examination. In an attempt to advance diagnostics, associations between specific contaminants and health of turtles in this region were tested. No comprehensive health assessments had been conducted at these sites prior to this study.The coastal Cleveland and Upstart Bays both demonstrated effects likely to be in response to stressors suspected to be anthropogenic in origin (elevated total white cell counts and creatinine kinase levels across the populations, respectively). This was associated with a suite of trace elements, in particular cobalt. While these indicators of stress resolved by the final year of the study, a chronic stressor was suspected to be persisting with ongoing low albumin: globulin. Necropsies did not elucidate any specific diseases.Although body condition index did not closely correlate with site health, barnacle counts in juvenile turtles may prove a reliable indicator of site health. Based on previously established indicators of poor health, barnacle counts showed that 10% of the population was in poor health at Upstart Bay and nearly 20% of the population at Cleveland Bay. This is above what would be expected for a normal population.Overall, the health component of this study suggested that the pristine turtle population was healthy and the coastal turtle populations were under active stressors, possibly caused by anthropogenic effectors such as chemical pollutants, when initially examined in 2014. These stressors resolved by the conclusion of the study in 2017; but chronic stressors remained absent in the pristine site and present within each of the studied coastal populations.

Green turtle (Chelonia mydas) population demographics at three chemically distinct foraging areas in the northern Great Barrier Reef

The catchments of the Great Barrier Reef (GBR) have experienced significant modifications in recent decades, leading to increases in sources of pollutants and declines in coastal water quality. As coastal waters of the GBR support some of the highest density green turtle (Chelonia mydas) foraging populations in the western Pacific Ocean, understanding the effects of contaminants on GBR green turtle populations is a priority. In 2012, elevated strandings of green turtles in the Upstart Bay region instigated the WWF's collaborative Rivers to Reef to Turtles (RRT) project to investigate if coastal pollutants are compromising green turtle health. Important to interpreting these investigations into toxicology and health is understanding the demographics of the green turtle populations being investigated. In three green turtle foraging grounds, Cleveland Bay (CLV), Upstart Bay (UPB) and the Howick Group of Reefs (HWK), this study explored population size, age class structure, sex ratio, growth rates, body condition and diet, as well as indices of turtle health, such as plastron barnacle loads and eye lesions. The three foraging populations had similar age class structure and adult sex ratios to other green turtle foraging populations in the GBR. Somatic growth rate was nonlinear, peaking in immature turtles, and was much slower in turtles foraging at HWK compared to the other two sites. This may have been due to differences in food source, which was supported by the observed dietary shifts between seagrass and algae in HWK turtles, compared to a consistently seagrass diet in CLV and UPB turtles. There were also small differences in body condition between sites, as well as differences in barnacle loads, eye lesions and occurrence of fibropapilloma tumors. This study provides important information on green turtle foraging ground population dynamics in the northern GBR, and context for the other papers in this special issue.

Stock structure of blue threadfin Eleutheronema tetradactylum on the Queensland east coast, as determined by parasites and conventional tagging

Blue threadfin Eleutheronema tetradactylum (Polynemidae) were examined from four areas (Princess Charlotte Bay, Trinity Inlet, Halifax Bay and Upstart Bay) in eastern Queensland covering a distance of c. 950 km of coastline. Parasites were used as biological markers to infer stock structure of E. tetradactylum. Parasites designated as 'temporary' biological markers were the copepod Thysanote eleutheronemi, the acanthocephalan Neoechinorhynchus topseyi, the nematode Philometra rajani and hemiurid trematodes. The larval nematodes Anisakis sp. Type 1 and Terranova sp. Type 2; and the larval cestodes Pterobothrium pearsoni and Callitetrarhynchus gracilis were considered 'permanent' biological markers. Both univariate and multivariate analyses demonstrated that there was little difference in temporary parasite abundance between the four areas. In contrast, the same analyses revealed that most areas had two or more significant differences in permanent parasite abundance, with the exception of Halifax Bay and Upstart Bay, which were significantly different only in the multivariate analysis. Biological markers predicted that Princess Charlotte Bay and Trinity Inlet consisted of distinct populations, whereas Halifax Bay and Upstart Bay were not clearly differentiated. Tag recapture data supported this hypothesis; the majority of recaptures were within 100 km of the initial tagging location. Geographical movement of E. tetradactylum may be limited due to their biology and ecology, as well as the distances and oceanographic boundaries that separate habitats. Contrary to current management definitions, the stock structure of E. tetradactylum on the east coast of Queensland appears to be geographically differentiated at a small spatial scale.

Sediment provenance in a tropical fluvial and marine context by magnetic ‘fingerprinting’ of transportable sand fractions

AbstractThe sources and fluxes of sediment to the Great Barrier Reef lagoon from north‐eastern Australian rivers have been the subject of much concern and study, with the large catchments of the Burdekin and Fitzroy Rivers thought to be the key sources at present. Here, the utility of newly developed magnetic ‘fingerprinting’ methods for identifying sediment provenance, both onshore and offshore, and in association with individual large flood events, is investigated. Within the Burdekin catchment, sediments are mobilized from different subcatchments by runoff generated by intense, localized rainfall events. Magnetic measurements were made on untreated and acid‐treated samples of river channel sediments within the Burdekin River subcatchments and from the estuarine and inner shelf depocentres of Burdekin River sediments. The acid treatment removes all discrete magnetic particles and coatings, and leaves magnetic inclusions (protected within host silicate grains) as the basis of the measured magnetic signature of a sample. The magnetic properties of the acid‐treated samples display statistically distinct sediment provenance groupings. Sand samples from the Upper Burdekin River appear magnetically distinct from samples from tributaries of the Burdekin (e.g. Hann Creek, Fanning River) and also from nearby coastal rivers, including the Haughton. Suspended sand samples from a Burdekin flood event in 2000 appear to have a different source compared with those from floods in 1998 and 1999. Comparisons of the terrestrial, acid‐treated sand fractions with the same, acid‐treated, sand‐size fractions from transects taken offshore suggest that the surface sediments in Upstart Bay and Bowling Green Bay have different sources. Some of these sources are as yet unidentified but may represent the unsampled, lower‐discharge south‐western Burdekin subcatchments, and/or along‐shore drift of sand from the south, perhaps even from the Fitzroy River, over millennial timescales of cyclone pumping. The magnetic inclusion method precludes any obfuscation or confounding of sediment source, which might arise from hydraulic sorting and/or post‐depositional magnetic diagenesis or authigenesis.

Preservation of genetic diversity in restocking of the sea cucumberHolothuria scabrainvestigated by allozyme electrophoresis

Population genetics analyses should be considered when releasing hatchery-produced juveniles of the sea cucumber Holothuria scabra when spawners from nonlocal populations are used. In New Caledonia, within-region genetic heterogeneity of H. scabra populations (examined through allozyme electrophoresis of 258 animals) indicated high gene flow between nine sites and FSTvalues did not deviate significantly from zero. However, exact tests indicated that populations at two sites with limited water exchange in the southern location were significantly different from populations at three other locations on the west coast. Inclusion of H. scabra sampled in Bali (n = 90) and Knocker Bay, Australia (n = 47), and comparisons with existing data from the west Pacific (Torres Strait, Solomon Islands, Upstart Bay, Hervey Bay) showed that populations were significantly different (using exact tests) and samples partitioned distinctly using unweighted pair group method with arithmetic mean clustering. Rogers' genetic distance values between populations were significantly related to geographic distances, showing a pattern of isolation by distance. The rapid increase in genetic distance over the first few hundred kilometres supports the view that the spatial extent of any translocation needs to be carefully considered on the basis of knowledge of variation in allele frequencies within the target area.

137Cs and excess 210Pb deposition patterns in estuarine and marine sediment in the central region of the Great Barrier Reef Lagoon, north-eastern Australia

This paper focuses on the distribution of 137Cs and 210Pb xs in 51 estuarine and marine sediment cores collected between the Upstart Bay and Rockingham Bay in the Great Barrier Reef Lagoon, north-eastern Australia. Historical records of 210Pb xs and 137Cs atmospheric deposition and present day terrestrial inventories in north-eastern Australia are presented. 210Pb xs and 137Cs fluxes measured on suspended sediments in the Burdekin River are considered to be a source of recent inputs of these nuclides to the nearshore region of this part of the Great Barrier Reef. Direct correlations between sediment nuclide inventories, maximum detectable depths, and sediment mass accumulation rates (MARs), calculated using both 137Cs and 210Pb xs, are explored. In relation to inventories of 210Pb xs, 60% of atmospheric fallout 137Cs appears to be missing from the sediments. The reasons for these differences in two tracers, primarily of atmospheric origin, are discussed in terms of the geochemical properties of these two nuclides. Evidence is presented to support the hypothesis that the 137Cs distribution in these cores can be a useful independent tracer which provides confirmation of MARs calculated from the decay of 210Pb xs.

Restricted gene flow between Holothuria scabra (Echinodermata: Holothuroidea) populations along the north-east coast of Australia and the Solomon Islands

To describe patterns of gene flow for the fished holothurian species Holothuria scabra 17 to 141 individuals were collected from 8 populations from north-east Australia, the Torres Strait, and the Solomon Islands. Samples were investigated by allozyme electrophoresis of 7 polymorphic loci. Cluster analyses using Rogers' genetic distance identified 3 distinct groups of populations from the north-east coast of Australia, representing samples from the 3 regions Hervey Bay, Upstart Bay and Torres Strait. Populations in the latter region were closely connected to those from the Solomon Islands. F-statistics indicated restrictions in gene flow (average genetic variation between popula- tions, FST = 0.088). Hierarchical analyses revealed that 94.7% of the variation was within sampling locations. Approximately 77% of the variance among populations was due to differences between regions, and 23% within regions (most of the latter caused by differences between the 2 Solomon Island populations). Mantel's tests indicated that a high proportion of the variation in genetic dis- tances along the north-east coast of Australia was explained by isolation by distance (Mantel's nor- malised Z = 0.88). This proportion reduced when the Solomon Islands were included (Z = 0.65). The detection of separate stocks along the north-east coast of Australia is an important finding that has significant consequences for the development of sustainable management plans for this species. Low dispersal may significantly reduce recovery of overfished areas if no local refugia are provided.

Coastal morphodynamics and the archaeological record: Further evidence from Upstart Bay, North Queensland

The principal aim of research at Upstart Bay, on the northeast Queensland coast, is to document the impact of tropical cyclones and concomitant storm surge on the coastal archaeological record. Baseline surveys at Upstart Bay in 1987 were followed by two cyclones in 1988 and 1989. The devastating impacts of these cyclones on coastal shell middens have been described in detail (Bird 1992). Annual surveys at Upstart Bay over the past seven years have highlighted the often dramatic changes which can occur in the archaeological record as a result of dynamic coastal geomorphic processes. Â Â

Seagrasses between Cape York and Hervey Bay, Queensland, Australia

The area of seagrasses in waters adjacent to the Queensland coast between Cape York and Hervey Bay is approximately 4000 km2. Seagrasses were found near estuaries, in coastal bays and associated with islands, at sites that provided shelter from the south-easterly trade winds and Pacific Ocean swells. Of the seagrass meadows mapped, 37% had a bottom vegetation cover greater than 50%. Two large continuous areas (total of approximately 2500 km2) of seagrass of predominantly Halophila species were found in deep water in Hervey Bay and between Barrow Point and Lookout Point and may be part of a much larger area of deep-water seagrass habitat not yet surveyed in the Great Barrier Reef province. Fourteen seagrass species were found in the surveyed region, and most were typical of the northern Australian and Indo-West Pacific region. The opportunistic Halophila and Halodule species were most common, with Halophila ovalis (R. Br.) Hook. f. and Halodule uninervis (Forsk.) Aschers. each being found in more than 15% of samples. High species richness occurred at depths of less than 6 m, predominantly in sheltered bays at coastal and island locations. Low species richness at estuary- associated sites may be due to stresses caused by low salinity during monsoonal runoff periods or exposure at low tides. Zostera capricorni Aschers. was restricted to these areas and may have a competitive advantage over other species with lesser tolerance to varying salinity. Species richness decreased with an increase in both latitude and depth. The latitudinal limits of recorded distributions for some of these tropical seagrasses were confirmed. Seagrass biomass decreased with increasing depth, but parameters of seagrass abundance showed no correlation with latitude, being dependent on a complex of site-related factors. High seagrass biomass occurred at sheltered sites, including estuary-associated, coastal-bay and island-associated sites. The maximum recorded above-ground biomass was 102.9 g m-2 for Zostera capricorni at Upstart Bay. Shoot densities reached 13 806 shoots m-2 for Halophila ovalis at Escape River, and the highest leaf area index was 1.81 for Zostera capricorni at Upstart Bay.

The impact of tropical cyclones on the archaeological record : an Australian example

AbstractThis paper describes the impact of two recent tropical cyclones on the coast and archaeological record at Upstart Bay, North Queensland. Resurveys of Upstart Bay following cyclone 'Charlie' in 1988 and cyclone 'Aivu' in 1989 indicate that considerable damage and even total destruction of late Holocene shell middens has occurred as a direct result of the destructive winds and storm surges associated with these cyclones. Of a total of 93 archaeological sites recorded at Upstart Bay during baseline studies in 1987, more than 50% have now been destroyed. This research raises questions about the effect of cyclones on site preservation throughout prehistory and their likely impact in the future under 'greenhouse' conditions. This study has important implications for the future management of cultural resources in the coastal zone.