Upogebia Pusilla Research Articles

Some biological aspects of Mediterranean mud shrimp, Upogebia pusilla (Petagna, 1792) in the Boka Kotorska Bay, Southeastern Adriatic Sea

Biological aspects (length frequency distribution, length-weight ratio, sex ratio, allometric growth, Fulton?s condition factor) of Upogebia pusilla were studied in Tivat Saline (Tivatska solila), Boka Kotorska Bay in 2019. A total of 1,415 individuals were collected. The total length of all studied individuals ranged from 27 to 55 mm (8 and 20 mm CL), while the total weights ranged from 0.29 to 2.56 g (the total length of males ranged from 27 to 51 mm, non-ovigerous and ovigerous females ranged from 27 to 51 mm and 30 to 55 mm, respectively; the weights ranged from 0.29 to 2.52 g, 0.34 to 2.52 g and 0.47 to 2.56 g for males, non-ovigerous and ovoid females, respectively). The overall female:male ratio was 1.2:1. Ovigerous females were recorded from April (74.3%) to June (23.8%), with a peak in May (91.4% of all females). Males dominated in July (66.4%). The relationship between CL and W was negatively allometric in both males and females and in all sampled individuals. Fulton?s condition factor was higher only in females during the breeding season, while there were no significant differences between males and females during the rest of the year. Since this species is mainly used as live bait for commercial and sports fishermen, the results of this study would be useful for the sustainable management of this species.

Biocoenotic and habitat ordination of mud shrimp Upogebia pusilla Petagna, 1792 (Crustacea: Decapoda) settlements on the northern part of the Black Sea shelf (Crimean coast)

Biocoenotic and habitat ordination of mud shrimp Upogebia pusilla Petagna, 1792 (Crustacea: Decapoda) settlements on the northern part of the Black Sea shelf (Crimean coast)

Burrowing mud shrimps (Decapoda: Axiidea amp; Gebiidea) of the northeastern Black Sea and their external parasites.

The article presents a brief review on the fauna of the axiidean and gebiidean burrowing mud shrimps and their external parasites in the northeastern coast of the Black Sea, where only two rather common species, Gilvossius candidus (Olivi, 1792) and Upogebia pusilla (Petagna, 1792) (Gebiidea: Upogebiidae), were previously recorded. Necallianassa truncata (Giard Bonnier, 1890) is confirmed and Gilvossius tyrrhenus (Petagna, 1792) (Axiidea: Callianassidae) is firstly recorded for the shallow waters of the northeastern Black Sea, where it was previously probably confused with common and widely distributed Gilvossius candidus (Olivi, 1792). Color photographs of the four species, enhancing the recognition of those species in fields, are provided.

Catching performance and catching efficiency of siliconized baits in hand line fishery

The present study investigated the catching performance and catching efficiency of natural mud shrimp (alive) (Upogebia pusilla), siliconized mud shrimp and siliconized pellet in handline fishing. The trials were performed in Kıyıkışlacık Village of Muğla province. The main body of the handline is Ø 0.50 mm, leader and snood are Ø 0.27-0.30 mm, the snood length and distance are 10 cm and 20 cm, respectively. Each handline has 3 hooks and the hook type is 4 no straight. Catches from natural mud shrimp, siliconized shrimp and siliconized pellet bait retained separately, sorted by species and weighed as. 0.01 g sensitivity and measured as the total length. Totally 590 individuals from 30 different species in total were caught including; 28 bony fish (93.33%), and 2 cephalopods (0.66%). While natural mud shrimp captured the 50.7% of fish, 44.7% and 4.6% were caught with siliconized mud shrimp and siliconized pellet, respectively. CPUE values of natural mud shrimp, siliconized shrimp and silicon pellet were calculated as 1.57 n/h, 1.38 n/h and 0.14 n/h, respectively. In addition, YPUE values were determined as 121.84 g/h, 137.73 g/h and 7.62 g/h for natural mud shrimp, siliconized shrimp and silicon pellet, respectively. Despite the fact that the number of individuals that a live mud shrimp catch in a unit of time is high, it was found out that the weight is more in a unit of time when silicone mud shrimp is used. It was concluded that using of siliconized mud shrimp has a high capacity of catching performance and catching efficiency and can be used as bait when natural mud shrimp cannot be utilized as alive or unable to be supplied, due to the weather conditions and time restrictions.

Paraketa balıkçılığında kullanılan farklı yemlerin avcılık performansı ve olta iğnesinde kalma oranı

In this study, the catch performance and remaining ratio on fishing hooks of natural alive mud shrimp (Upogebia pusilla) (CCK), siliconized mud shrimp (SCK) and siliconized pellet (SP) were investigated in a longline. The trials were carried out monthly between May 2016 and April 2017 in Zeytinlikuyu district of Kıyıkislacik Village – Milas-Mugla province. The main body diameter has 0.70 mm, leader and snoods have 0.40 mm. The snood length and distance is 1.5 m and 4.0 m, respectively. 14 no straight hooks were used. Totally 150 hooks, 50 for each bait were used and each bait was replaced in 25 hooks. The individuals caught during the samplings were separated as CCK, SCK and SP, and each individual was measured as the precision of mm the total length (TL) and weighted of 0.01 g. Totally 191 individuals from seven families with 18 different species were caught. Gilthead sea bream (Sparus aurata) was the abundant species with 48.7% in all bait type, and the followed by common pandora (Pagellus erythrinus) with 10.5%, common two-banded sea bream (Diplodus vulgaris) with 9.4% and annular sea bream (Diplodus annularis) with 8.9%. Of the 191 individuals, 55% (105 individuals) were caught with CCK, 33.5% (64 individuals) with SCK and 11.5% (22 individuals) with SP. The results of the trial with 3 different bait types were shoved that that there are significant differences between the catch rates of gilthead sea bream, annular and common two-banded sea bream (χ2, P<0.05). In paired tests between bait groups (CCK-SCK, CCK-SP and SCK-SP), there were no clear differences between the sizes of the individuals (ANOVA, P>0.05). Catch per unit effort (CPUE) values of CCK, SCK and SP were calculated as 0.05 n/h, 0.03 n/h and 0.01 n/h, respectively. In the same order, yield per unit effort (YPUE) values were determined as 7.28 g/h, 4.35 g/h and 2.99 g/h. When the remaining rate of baits on the hooks were evaluated, CCK gave the lowest rate with 6.32%2.01. It is thought that SCK should be used as an alternative particularly when natural mud shrimp cannot be utilized as alive or unable to be supplied due to the cold weather conditions and time restrictions. On the other hand, it is thought that performing such studies with bait made with natural binders such as gelatine instead of silicone will be extremely important in terms of less harm to both fish and nature.

Parasitism in ecosystem engineer species: A key factor controlling marine ecosystem functioning.

Although parasites represent a substantial part of marine communities' biomass and diversity, their influence on ecosystem functioning, especially via the modification of host behaviour, remains largely unknown. Here, we explored the effects of the bopyrid ectoparasite Gyge branchialis on the engineering activities of the thalassinid crustacean Upogebia pusilla and the cascading effects on intertidal ecosystem processes (e.g. sediment bioturbation) and functions (e.g. nutrient regeneration). Laboratory experiments revealed that the overall activity level of parasitized mud shrimp is reduced by a factor 3.3 due to a decrease in time allocated to burrowing and ventilating activities (by factors 1.9 and 2.9, respectively). Decrease in activity level led to strong reductions of bioturbation rates and biogeochemical fluxes at the sediment-water interface. Given the world-wide distribution of mud shrimp and their key role in biogeochemical processes, parasite-mediated alteration of their engineering behaviour has undoubtedly broad ecological impacts on marine coastal systems functioning. Our results illustrate further the need to consider host-parasite interactions (including trait-mediated indirect effects) when assessing the contribution of species to ecosystem properties, functions and services.

Linking behaviours, sediment reworking, bioirrigation and oxygen dynamics in a soft-bottom ecosystem engineer: The mud shrimp Upogebia pusilla (Petagna 1792)

Despite the role of bioturbation in controlling biogeochemical processes taking place at the sediment-water interface, little is still known about how animal behaviours influence sedimentary biogeochemical processes. In the present work, we used the mud shrimp Upogebia pusilla as model organism to assess the effect of behaviour types/intensities on sediment reworking, bioirrigation and sediment oxygen dynamics based on three sets of seasonal experiments. We identified four main behaviours: “Resting”, “Walking”, “Ventilating” and “Burrowing”. Mud shrimp were much more active (i.e., not “Resting”) during summer than during winter and to a lesser extent spring due to elevated temperature. There were three distinct components of sediment reworking. The first one consisted in the expulsion of sediment particles from the burrow to the sediment-water interface, the second one to the transfer of sediment downward within the burrow and the third one to the transport and compaction of sediment across burrow walls. Temporal changes in the intensities of all components correlated tightly since they all resulted from “Burrowing”. The temporal dynamics of porewater exchange and oxygen dynamics were clearly discontinuous and in direct relation with “Ventilating”. However, other behaviours, and especially “Burrowing” were also involved in the control of this dynamics through the modulation of oxygen availability within the burrow, which is cuing the onset of ventilation at a 12–25% saturation threshold. These results underline the importance of taking into account changes in animal behaviour types/intensities at the appropriate temporal scales when assessing the impact of bioturbation on biogeochemical processes taking place at the sediment-water interface.

Physical-biological interactions in the ocean: case studies from the northern Canary Current Upwelling Ecosystem

Physical-biological interactions in the ocean: case studies from the northern Canary Current Upwelling Ecosystem

Seasonal abundance, vertical distribution and life history traits of Mediterranean mud shrimp Upogebia pusilla (Decapoda: Gebiidea) on the Slovenian coast

We studied seasonal abundance, vertical distribution and life history traits of the Mediterranean mud shrimp (Upogebia pusilla) in the mediolittoral zone in Slovenia. At two localities (Strunjan and Lazaret), quantitative samples from three plots (1 m2 each) at (0 cm), below (-50 cm) and above (+50 cm) mean water level were taken once every two months, in alternation. A set of parameters was also recorded, including vegetation cover, sediment type, air and sea temperature, pH, salinity and oxygen levels. Highest abundance was recorded during the warm period of the year, with peak abundance in June 2012 (21 % of total sample). Lowest plots were always occupied with the highest densities of mud shrimps. Males dominated during the winter while ovigerous females reached the peak in June (>40 % of all animals). In June at least 2/3 of females were ovigerous. Estimated mean incubation period was 31 days. We determined four periods in relation to the species’ reproductive activity. Early reproductive period (April) was characterised by emergence of first ovigerous females, while main reproductive period (May–July) was characterised by peak of reproduction. In post-reproductive (August–October), and non-reproductive (November–March) periods, no ovigerous females or newly hatched offspring were recorded.

Parasite interactions in the bioturbator Upogebia pusilla (Decapoda: Gebiidae): a case of amensalism?

The mud shrimp Upogebia pusilla is a deep-burrowing bioturbator widespread along the Northeast Atlantic and Mediterranean coasts, where it is recognized as an ecosystem engineer. Parasitism is an important factor impacting such species’ activities, and thus ecosystem functioning. At least two parasite species occur in U. pusilla: the bopyrid isopod Gyge branchialis and an undescribed trematode. This study, carried out in Arcachon Bay, France (44°40′N, 1°10′W), over 2 years (2014–2015), had two goals: (1) to identify the trematode occurring in U. pusilla and (2) to assess the interactions between the two parasites within their host. Using molecular techniques, the trematode was identified as Maritrema sp. (Microphallidae). Monthly samples taken over 2 years at a single site, and a ten-site spatial survey in June 2014 showed that there was a negative association in the occurrence of the two parasites in their host over time and amongst sites: bopyrid-infested mud shrimp harbored lower trematode infections compared to bopyrid-free individuals. In addition, the abundance of trematodes was lower in the bopyrid-infested gill compared to the uninfested gill of bopyrid-infested individuals. It suggests that G. branchialis interferes with Maritrema sp. establishment in the mud shrimp. Conversely, the trematode appeared not to have a negative effect on bopyrid presence. The reduction of Maritrema sp. infection by G. branchialis is mainly due to the alteration of U. pusilla fitness (indirect interaction), and to a lesser extent, to overlap of parasites’ niches (direct interaction). Accordingly, interactions between these two parasites can be classified as amensalism.

Dynamics of the Upogebia pusilla–Gyge branchialis marine host–parasite system

The influence of burrowing invertebrates on the functioning of soft-bottom ecosystems is tightly dependent on their physiological state, which may itself be strongly affected by parasite infestation. However, the dynamics, prevalence and physiological consequences of parasitism remain poorly known in many species, such as sediment-dwelling crustaceans. During the present study, we investigated the effects of the ectoparasite (bopyrid) isopod Gyge branchialis on the fitness of the gebiidean mud shrimp Upogebia pusilla. A spatial survey conducted in June 2013 at 10 stations scattered across intertidal seagrass meadows of Arcachon Bay (44°40′N, 1°10′W) showed that on average 16 % of mud shrimp were infested by G. branchialis. Moreover, a temporal survey carried out at one of these stations over the year 2014 revealed that prevalence remained relatively high throughout the year, ranging from 10 to 33 %. This interaction has various physiological consequences for U. pusilla. In particular, infested mud shrimp consistently exhibited a significant deficit in total length and individual dry weight as compared with non-infested specimens. Moreover, 89 % of infested males were feminized (i.e., developed female secondary sexual characters), whereas infested females were never ovigerous. Our results demonstrate that G. branchialis strongly impair U. pusilla fitness including its ability to reproduce. Accordingly, through their (1) potential impact on mud shrimp abundances and (2) alteration of their physiological state, high bopyrid infestation could profoundly alter the bioturbation activity of mud shrimp populations and overall their influence on the functioning of soft-bottom ecosystems in Arcachon Bay.

Heteromysis (Heteromysis) microps (Crustacea, Mysidae), a commensal species for Upogebia pusilla (Crustacea, Upogebiidae) in Arcachon Bay (NE Atlantic Ocean)

The mysid Heteromysis (Heteromysis) microps is reported for the first time in the Bay of Biscay. During surveys carried out between March and September 2015 in Arcachon Bay, mysid specimens were fortuitously collected from Upogebia pusilla burrows. Details on morphology, colour pattern and behaviour of H. microps are provided. Commensalism was frequently mentioned for Heteromysis species but never reported for H. microps. In this study, commensalism seems to be proved between the mysid and its host U. pusilla.

Influence of the mud shrimp Upogebia pusilla (Decapoda: Gebiidea) on solute and porewater exchanges in an intertidal seagrass (Zostera noltei) meadow of Arcachon Bay: An experimental assessment

The aim of the present study was to investigate the influence of the mud shrimp Upogebia pusilla on porewater and solute exchanges in an intertidal Zostera noltei meadow of Arcachon Bay. Laboratory experiments carried out during three seasons showed that U. pusilla strongly enhanced sediment porewater exchange rates (averaging 26, 45 and 71Lm−2d−1 in winter, spring and summer, respectively) in comparison to uninhabited sediments for which these rates were moderate (averaging 9, 9 and 16Lm−2d−1 in winter, spring and summer, respectively). Total Oxygen Uptakes (TOUs) were increased by factors around 2.5 during the three studied seasons whilst oxygen consumption by the shrimp accounted for 64, 28 and 21% of TOU in winter, spring and summer, respectively. U. pusilla also significantly enhanced the uptake of nitrate (up to 13.8-fold in summer) and the release of ammonium (up to 3.0-fold in summer) and silicate (up to 3.8-fold in spring) by the sediment. Overall, our results highlight the key role of U. pusilla in controlling oxygen and nutrient fluxes in Arcachon Bay. Because U. pusilla populations are predominantly found in the sediments of Z. noltei meadows, the full assessment of the functional effects resulting from the decline of this meadow should include the effects associated to the disappearance of U. pusilla as well.

Solution structures of 2 × 6-meric and 4 × 6-meric hemocyanins of crustaceans Carcinus aestuarii, Squilla mantis and Upogebia pusilla

Arthropod hemocyanins (Hcs) are a family of large, high molecular mass, extracellular oxygen transport proteins. They form oligomeric quaternary structures based on different arrangements of a basic 6×75 kDa hexameric unit. Their complex quaternary structures present binding sites for allosteric effectors and regulate the oxygen binding process in a cooperative manner. In order to describe the functional regulation of arthropod Hcs, a detailed description of their quaternary structure is necessary. We have utilized small angle X-ray scattering to characterize the structure of three arthropod Hcs in unperturbed conditions. Two different levels of complexity are evaluated: for the 2×6-meric case, we analyzed the Hcs of the portunid crab Carcinus aestuarii and stomatopod Squilla mantis, while in the case of 4×6-meric structures, we studied the Hc of the thalassinid shrimp Upogebia pusilla. While C. aestuarii Hc presented a structure comparable to other 2×6-meric crustacean Hcs, S. mantis Hc shows a peculiar and quite unique arrangement of its building blocks, resembling a substructure of giant Hcs found among cheliceratans. For U. pusilla, the arrangement of its subunits is described as tetrahedral, in contrast to the more common square planar 4×6-meric structure found in other arthropod Hcs.

Unusual oxygen binding behavior of a 24-meric crustacean hemocyanin

Hemocyanins from Crustacea usually are found as 1x6 or 2x6-meric assemblies. An exception is the hemocyanin isolated from thalassinidean shrimps where the main component is a 24-meric structure. Our analysis of oxygen binding data of the thalassinidean shrimp Upogebia pusilla based on a three-state MWC-model revealed that despite the 24-meric structure the functional properties can be described very well based on the hexamer as allosteric unit. In contrast to the hemocyanins from other thalassinidean shrimps the oxygen affinity of hemocyanin from U. pusilla is increased upon addition of l-lactate. A particular feature of this hemocyanin seems to be that l-lactate already enhances oxygen affinity under resting conditions which possibly compensates the rather low intrinsic affinity observed in absence of l-lactate. The fast rate of oxygen dissociation might indicate that in this hemocyanin a higher cooperativity is less important than a fast response of saturation level to changes in oxygen concentration.

Brachionus vs Artemia duel: Optimizing first feeding of Upogebia pusilla (Decapoda: Thalassinidea) larvae

Larval rearing of many marine organisms is dependent on the availability of live food. The aim of this study was to optimize larval first feeding for the mud shrimp Upogebia pusilla, by comparing the effectiveness of the two most commonly used live feeds: Brachionus plicatilis and Artemia sp. nauplii. Survival, larval duration, molt synchronism and megalop size were compared using five feeding treatments: Artemia from zoea I to IV (B0), Brachionus during zoea I and Artemia from zoea II to IV (B1), Brachionus during zoea I and II and Artemia during zoea III and IV (B2), Brachionus from zoea I to III and Artemia during zoea IV (B3) and Brachionus from zoea I to IV (B4). The proportion of larvae that reached the megalop stage was 0.00% in treatment B0, 3.33% in treatment B1, 33.33% in treatment B2, 66.67% in treatment B3 and 76.67% in treatment B4. Larvae fed on rotifers until zoea III or zoea IV stages had a higher survival but no differences were found either in time to reach megalop or in megalop size. This study demonstrates that rotifers are essential for the survival and development of U. pusilla early larval stages but that rotifers can be successfully replaced by Artemia nauplii in the zoea IV stage.

Feeding ecology of thornback ray (Raja clavata Linnaeus, 1758) on the Turkish coast of the south-eastern Black Sea

The feeding ecology of the thornback ray, Raja clavata, was studied based on the analysis of the stomach contents of specimens caught in the south-eastern Black Sea, Turkey, between May 2003 and January 2004. A total of 198 thornback rays were analysed to describe food habits and investigate the effect of sex, size and season on diet. Thornback rays fed mainly on teleost fish (especially red mullet, Mullus barbatus ponticus) and mud shrimp (Upogebia pusilla). Other prey included harbour crab, whiting, shrimp larvae and others. No differences were observed between dietary composition of males and females, but significant differences were found between size classes and seasons.

The molecular heterogeneity of hemocyanin: Structural and functional properties of the 4 × 6-meric protein of Upogebia pusilla (Crustacea)

The structural properties of the hemocyanin isolated from the Mediterranean mud shrimp, Upogebia pusilla (Decapoda: Thalassinidea), were investigated. Our intent was to make use of the U. pusilla case to perform a structural comparison between crustacean and chelicerate 4 × 6-meric hemocyanins. The thalassinidean hemocyanin appears similar in size but different in structural organization compared to the chelicerate 4 × 6-mer. Ultracentrifuge analyses on the purified protein revealed a sedimentation coefficient of 39S, typical of 4 × 6 hemocyanins. Electron micrographs are in agreement with a model in which four 2 × 6-meric building blocks are arranged in a tetrahedron-like quaternary structure and not in the quasi-square-planar orientation characteristic of the chelicerate protein. Size-exclusion chromatography-fast protein chromatography analysis showed elevated instability of the protein in absence of divalent ions or at pH values higher than 8.0. This analysis also shows that the dissociation of the U. pusilla 4 × 6-meric hemocyanin into hexamers occurs without any intermediate 2 × 6-meric state, in contrast with the dissociation profile of the chelicerate protein exhibiting several dissociation intermediates. The oxygen-binding properties of U. pusilla hemocyanin were studied to disclose possible effects by the typical allosteric effectors that modulate the functional properties of crustacean hemocyanin. A marked Bohr and lactate effect, but no significant influence of urate, on the oxygen affinity of U. pusilla hemocyanin were found.

Orientation of crustacean burrows in the Bay of Panzano (Gulf of Trieste, Northern Adriatic Sea)

Three burrow systems produced by Jaxea nocturna and one cluster of burrows produced by Upogebia pusilla where investigated in the Bay of Panzano, Northern Adriatic Sea, to determine preferred orientations. The distributions of dip directions differ between both producers. Steep shafts into the consolidated mud followed by large, shallowly inclined tunnels oriented in a manner similar to a spiral are characteristic for Jaxea nocturna burrows. In contrast, the Y-shaped burrows of Upogebia pusilla have entrance shafts that are less steep which are connected by a near-horizontal section, where a blind ending tunnel branches. Orientations of the dominating, shallowly inclined burrow parts are not randomly distributed in either Jaxea or in Upogebia burrows. Three preferred axial orientations with almost equal proportions in each direction are characteristic for the Jaxea burrow from the centre of the bay. This system transforms to a squared structure near the coast, where one direction parallels the shoreline and the other is oriented at right angles to the coast. The latter orientation demonstrates significant constancy in all investigated burrows. Upogebia burrow clusters coincide with the three preferred orientations of the Jaxea burrow from the bay centre, but the proportions of the directions are unequal. Burrow segments connecting the steep entrance shafts in Upogebia are oriented almost parallel to the shoreline, whereas at right angles to the coast the flat blind-ending tunnels incline towards the open sea. The study shows a strong coincidence between empirical and theoretical distributions of the dominating orientations in both species.

Caging experiment: Relationship between mesh size and artifacts

In marine sediments, cages are widely used to detect effects of predation on infauna. Artifacts due to the use of cages have been, instead, poorly estimated. In this study we have analysed the changes in macrofauna and in sediment composition when cages of different mesh sizes are used to exclude predators. In a shallow micro-tidal flat, the effect of three mesh sizes (2, 6 and 18 mm) was tested on sediment and macrofauna, using cages that excluded predators (EC), partial cages (PC) and natural sediment (un-caged plots, UC). PC allowed the predators to feed on the sediment, but modified the sediment similarly to the complete cages. Changes were measured for the abundance and species composition of macrofauna and for sediment total organic matter (TOM), chlorophyll- a, proteins and water content. The use of cages did not alter TOM and water content, whereas chlorophyll- a and phaeopigments were more abundant under the cages. The deep-burrowing shrimp Upogebia pusilla also increased under cages of any mesh-size. There was, instead, an effect on the abundance of macrofauna only when the smallest mesh size was used. Surprisingly, the exclusion of predators decreased the abundances of Tanais dulongii, Oligochaetes, Brania oculata, whereas the abundance of Aphelochaeta marionii changed when both PC and EC were put in the sediment. Collectively, all these findings indicated that for surface animals the use of small mesh sizes can bias the interpretation of results.