Root foundation is a new type of bio-inspired foundation, which has a broad application prospect in the development and construction of China's South China Sea area due to its good bearing characteristics such as pullout bearing capacity. The effect of root buried depth on the uplift bearing characteristics of root piles is analyzed through the model test of uplift bearing of root piles with different root buried depths in coral sand foundation. The results show that increasing the root buried depth can improve the ability of the root pile to control the uplift displacement, and there exists an optimal buried depth or range of buried depths that can effectively improve the pile foundation's uplift bearing capacity and control the ultimate displacement. The attenuation of axial force at the root is in the form of a step; with the increase of root buried depth, the maximum value of lateral friction resistance develops from the lower part of the pile body without root to the upper part of the pile body without root. The range of the bearing ratio of the root section of the pile with root buried depth of 260mm, 340mm and 420mm is 12.33%-15.68%, 9.98%-17.82% and 7.61%-21.65%, respectively; the smaller the root buried depth is, the higher is the ratio of the bearing ratio of the root section at the beginning of loading, and the bigger the root buried depth is, the bigger is the ratio of the root's final bearing ratio. The change of soil pressure around the pile increases and then decreases, and the densest point of soil compacting moves upward with the increase of root buried depth. The research results provide scientific basis for the design of root pile buried depth and root arrangement in actual projects.