You have accessJournal of UrologyUrodynamics/Incontinence/Female Urology: Basic Research II1 Apr 2012106 FUNCTIONAL ROLES OF TRANSIENT RECEPTOR POTENTIAL MELASTATIN (TRPM) 8 CHANNEL BLOCKER INHIBITS COLD STRESS-INDUCED DETRUSOR OVERACTIVITY IN AWAKE CONSCIOUS RATS Zhang Lei, Tetsuya Imamura, Osamu Ishizuka, Yoshiki Kurizaki, Hitoshi Yokoyama, Wataru Noguchi, Takahiro Yamagishi, and Osamu Nishizawa Zhang LeiZhang Lei Matsumoto, Japan More articles by this author , Tetsuya ImamuraTetsuya Imamura Matsumoto, Japan More articles by this author , Osamu IshizukaOsamu Ishizuka Matsumoto, Japan More articles by this author , Yoshiki KurizakiYoshiki Kurizaki Matsumoto, Japan More articles by this author , Hitoshi YokoyamaHitoshi Yokoyama Matsumoto, Japan More articles by this author , Wataru NoguchiWataru Noguchi Matsumoto, Japan More articles by this author , Takahiro YamagishiTakahiro Yamagishi Matsumoto, Japan More articles by this author , and Osamu NishizawaOsamu Nishizawa Matsumoto, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.154AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES We showed that rats suddenly exposed with low temperature exhibited cold stress-induced detrusor overactivity. The reactions were related with a resiniferatoxin-sensitive neurological pathway through a Transient Receptor Potential Melastatin (TRPM) 8 channel (Imamura, et al. Neurourol Urodyn, 2008; Chen, et al. Neurourol Urodyn, 2009). In this study, we determined if TRPM8 blocker inhibited the cold stress-induced detrusor overactivity. METHODS Twelve female 10-weeks Sprague-Dawley rats were cannulated. At 2 days after, continuous cystometric investigations were performed with unanesthetized and unrestricted rats under two ambient temperature conditions: room temperature (RT, 28±2°C) for 20 min and low temperature (LT, 4±2°C) for 40 min. Ten minutes prior to transferring to LT condition, the 6 rats were intravenously administrated with 20μM/100μl/200g-body weight TRPM8 blocker (BCTC). Other rats were similarly injected saline, as control. During each exposure, cystometric patterns were recorded. The LT exposure period was divided into 20 min phases (Cold Phase I and II). RESULTS During the first 20 min of cold exposure, phase I, voiding interval and bladder capacity of the BCTC-free control rats (1.19±0.18 min and 0.40±0.05 ml) significantly decreased compared to RT (3.08±0.41 min and 0.56±0.07 ml, P<0.05; Fig.1). During the second 20 min of exposure, phase II, the both values were significantly increased (2.28±0.29 min and 0.46±0.06 ml, P<0.05). In contrast, the voiding interval and bladder capacity of BCTC-treated rats in the both cold phases (3.21±0.27 min and 0.57±0.06 ml, 3.76±0.48 min and 0.66±0.08 ml, respectively) were as same level as in the RT condition (3.52±0.55 min and 0.65±0.10 ml; Fig.1). There were no significant differences between the groups under RT condition. However, the voiding interval and bladder capacity of BCTC-treated rats in the both cold phases were significantly higher than these of control ones (P<0.01). CONCLUSIONS The BCTC-treated rats did not exhibited decreases of the voiding interval and bladder capacity consistent with detrusor overactivity under LT condition. Therefore, BCTC partially inhibits cold stress-induced detrusor activity. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e43 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Zhang Lei Matsumoto, Japan More articles by this author Tetsuya Imamura Matsumoto, Japan More articles by this author Osamu Ishizuka Matsumoto, Japan More articles by this author Yoshiki Kurizaki Matsumoto, Japan More articles by this author Hitoshi Yokoyama Matsumoto, Japan More articles by this author Wataru Noguchi Matsumoto, Japan More articles by this author Takahiro Yamagishi Matsumoto, Japan More articles by this author Osamu Nishizawa Matsumoto, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...
Read full abstract
Oct 11, 2024
Sadahiko Nakajima 
Open Access