Unknown Members Research Articles

Design, Immunogenicity and Preclinical Efficacy of the ChAdOx1.COVconsv12 Pan-Sarbecovirus T-Cell Vaccine.

During the COVID-19 pandemic, antibody-based vaccines targeting the SARS-CoV-2 spike glycoprotein were the focus for development because neutralizing antibodies were associated with protection against the SARS-CoV-2 infection pre-clinically and in humans. While deploying these spike-based vaccines saved millions of lives worldwide, it has become clear that the immunological mechanisms of protection against severe disease are multifaceted and involve non-neutralizing antibody components. Here, we describe a novel pan-sarbecovirus T-cell vaccine, ChAdOx1.COVconsv12, designed to complement and broaden the protection of spike vaccines. The vaccine immunogen COVconsv12 employs the two regions in the viral proteome most conserved among sarbecoviruses, which are delivered by replication-deficient vector ChAdOx1. It directs T cells towards epitopes shared among sarbecoviruses including evolving SARS-CoV-2 variants. Here, we show that ChAdOx1.COVconsv12 induced broad T-cell responses in the BALB/c and C57BL/6 mice. In the Syrian hamster challenge model, ChAdOx1.COVconsv12 alone did not protect against the SARS-CoV-2 infection, but when co-administered with 1/50th of the ChAdOx1 nCoV-19 spike vaccine protective dose, faster recovery and lower oral swab viral load were observed. Induction of CD8+ T cells may decrease COVID-19 severity and extend the T-cell response coverage of variants to match the known (and as yet unknown) members of the β-coronavirus family.

PSVI-6 Metabolic potential of three novel rumen bacterial species from sheep characterized by utilizing a metagenomic-based approach

Abstract The sheep rumen microbiome contains a vast wealth of known and unknown members, whose metabolic processes impact nutrient acquisition as well as gut and overall health of the animal. Based on previous research from our group on investigating the impact of a change in diet on the composition of the rumen microbiome in lambs, further insights into the metabolic functions of novel bacterial species represented by three Operational Taxonomic Units (OTUs) were pursued. The rumen fluid samples with the greatest abundance of OTUs RA1-01223, RA1-00032, and RA1-04216 were, respectively, used as representatives for metagenomic investigations to assemble the genomes of their corresponding bacterial species. Purified microbial genomic DNA was used as a template for ‘shotgun’ sequencing with an Illumina Miseq (2 x 250) platform. Raw datasets were filtered for quality then used for contig assembly with an in-house developed set of Perl scripts, generating 64 contigs for RA1-01223 [combined total length: 2,046,349 base pairs (bp)], 118 contigs for RA1-00032 (combined total length: 3,704,255 bp), and 119 contigs for RA1-04216 (combined total length: 8,884,743 bp). After building, coding sequences were identified and annotated using RAST, with metabolic pathways of predicted enzymes constructed for each OTU of interest using KEGG pathways as a model reference. Annotated coding sequences were also screened by blastp to assign contig sets to each OTU based on their respective taxonomic affiliation; both OTU RA1-01223 and OTU RA1-00032 were found to be most closely related to Ruminococcus bili (94.8% and 86.8%, respectively), while RA1-04216 was affiliated to Succinivibrio dextrinsolvens (96.9%). As RA1-01223 and RA1-00032 were found to possess enzymes responsible for lactate metabolism, they could potentially affect susceptibility to acidosis by acting as modulators of ruminal pH or by producing lactate from starch degradation. Distinguishing features between these two OTUS included the pathway for vitamin B5 synthesis, using uracil or L-aspartate as precursors (RA1-01223), and the ability to produce ethanol as an end product of ruminal fermentation (RA1-00032). Distinguishing metabolic activities of interest for RA1-04216 included synthesis of chorismate and prephenate, which are precursors for tryptophan and phenylalanine, respectively. Together, these results contribute to a better understanding of the metabolic roles of individual microorganisms in the rumen, providing information that is crucial for creating a healthy and proactive environment that benefits the host.

Valence-Dependent Implicit Action Generalization Among Group Members.

People implicitly generalize the actions of known individuals in a social group to unknown members. However, actions have social goals and evaluative valences, and the extent to which actions with different valences (helpful and harmful) are implicitly generalized among group members remains unclear. We used computer animations to simulate social group actions, where helping and hindering actions were represented by aiding and obstructing another's climb up a hill. Study 1 found that helpful actions are implicitly expected to be shared among members of the same group but not among members of different groups, but no such effect was found for harmful actions. This suggests that helpful actions are more likely than harmful actions to be implicitly generalized to group members. This finding was replicated in Study 2 by increasing the group size from three to five. Study 3 found that the null effect for generalizing harmful actions among group members is not due to the difficulty of detecting action generalization, as both helpful and harmful actions are similarly generalized within particular individuals. Moreover, Study 4 demonstrated that weakening social group information resulted in the absence of implicit generalization for helpful actions, suggesting the specificity of group membership. Study 5 revealed that the generalization of helping actions occurred when actions were performed by multiple group members rather than being repeated by one group member, showing group-based inductive generalization. Overall, these findings support valence-dependent implicit action generalization among group members. This implies that people may possess different knowledge regarding valenced actions on category-based generalization.

Establishment of a non-Westernized gut microbiota in men who have sex with men is associated with sexual practices

The human gut microbiota is influenced by various factors, including health status and environmental conditions, yet considerable inter-individual differences remain unexplained. Previous studies identified that the gut microbiota of men who have sex with men (MSM) is distinct from that of non-MSM. Here, we reveal through species-level microbiota analysis using shotgun metagenomics that the gut microbiota of many MSM with Western origin resembles gut microbial communities of non-Westernized populations. Specifically, MSM gut microbiomes are frequently dominated by members of the Prevotellaceae family, including co-colonization of species from the Segatella copri complex and unknown Prevotellaceae members. Questionnaire-based analysis exploring inter-individual differences in MSM links specific sexual practices to microbiota composition. Moreover, machine learning identifies microbial features associated with sexual activities in MSM. Together, this study shows associations of sexual activities with gut microbiome alterations in MSM, which may have a large impact on population-based microbiota studies.

The Microbial Diversity on the Surface of Smear-Ripened Cheeses and Its Impact on Cheese Quality and Safety.

Smear-ripened cheeses are characterized by a viscous, red-orange surface smear on their rind. It is the complex surface microbiota on the cheese rind that is responsible for the characteristic appearance of this cheese type, but also for the wide range of flavors and textures of the many varieties of smear-ripened cheeses. The surface smear microbiota also represents an important line of defense against the colonization with undesirable microorganisms through various types of interaction, such as competitive exclusion or production of antimicrobial substances. Predominant members of the surface smear microbiota are salt-tolerant yeast and bacteria of the phyla Actinobacteria, Firmicutes, and Proteobacteria. In the past, classical culture-based approaches already shed light on the composition and succession of microorganisms and their individual contribution to the typicity of this cheese type. However, during the last decade, the introduction and application of novel molecular approaches with high-resolution power provided further in-depth analysis and, thus, a much more detailed view of the composition, structure, and diversity of the cheese smear microbiota. This led to abundant novel knowledge, such as the identification of so far unknown community members. Hence, this review is summarizing the current knowledge of the diversity of the surface smear microbiota and its contribution to the quality and safety of smear-ripened cheese. If the succession or composition of the surface-smear microbiota is disturbed, cheese smear defects might occur, which may promote food safety issues. Hence, the discussion of cheese smear defects in the context of an increased understanding of the intricate surface smear ecosystem in this review may not only help in troubleshooting and quality control but also paves the way for innovations that can lead to safer, more consistent, and higher-quality smear-ripened cheeses.

Enrichment of different taxa of the enigmatic candidate phyla radiation bacteria using a novel picolitre droplet technique.

The candidate phyla radiation (CPR) represents a distinct monophyletic clade and constitutes a major portion of the tree of life. Extensive efforts have focused on deciphering the functional diversity of its members, primarily using sequencing-based techniques. However, cultivation success remains scarce, presenting a significant challenge, particularly in CPR-dominated groundwater microbiomes characterized by low biomass. Here, we employ an advanced high-throughput droplet microfluidics technique to enrich CPR taxa from groundwater. Utilizing a low-volume filtration approach, we successfully harvested a microbiome resembling the original groundwater microbial community. We assessed CPR enrichment in droplet and aqueous bulk cultivation for 30days using a novel CPR-specific primer to rapidly track the CPR fraction through the cultivation attempts. The combination of soil extract and microbial-derived necromass provided the most supportive conditions for CPR enrichment. Employing these supplemented conditions, droplet cultivation proved superior to bulk cultivation, resulting in up to a 13-fold CPR enrichment compared to a 1- to 2-fold increase in bulk cultivation. Amplicon sequencing revealed 10 significantly enriched CPR orders. The highest enrichment in CPRs was observed for some unknown members of the Parcubacteria order, Cand. Jorgensenbacteria, and unclassified UBA9983. Furthermore, we identified co-enriched putative host taxa, which may guide more targeted CPR isolation approaches in subsequent investigations.

Measuring the Impact of Ease of Use, Usefulness, Attitude, and Enjoyment toward Intention to Play PUBG Mobile

The impact of ease of use, usefulness, attitude, and enjoyment toward intention to play PUBG Mobile as it relates to Indonesians, is explicitly examined in this study’s examination of the mobile gaming era because of the pandemic. This paper investigates the factors that influence an individual’s intention to play PUBG Mobile and the outcome of those factors towards its individuals, as well as the effects of knowledge about gaming behavior to its users. The study’s recommendations are to increase the intention to play mobile games so that the need for enjoyable aspects and the need for outside assistance become more apparent. Through an online survey, 223 individuals provided information for this study. The survey asked for responses from people who had played a mobile game, particularly Player Unknown Battle Ground Mobile version (PUBGM), millennials or members of Generation Z having enrolled in college and who previously had resided in Indonesia, had at least played PUBG Mobile in the past, and had played it more than one year. In this work, statistical analysis is carried out. The outcome demonstrates that perceived ease of use, usefulness, attitude, and perceived enjoyment have an impact toward Intention to play PUBG Mobile. This study closes a gap in the literature by concentrating on the behavioral intention to play PUBG through the playful-consumption experiences of a well-known online multiplayer game.

Robinson, Rachel Elizabeth. Visual and Plastic Poetics: From Brazilian Concretism to the Chilean Neo-Avant-Garde

This book focuses on three relatively unknown members of the Chilean neo-avant-garde and their debt to Brazilian concrete poetry: Juan Luis Martínez, Rodrigo Lira and Cecilia Vicuña. Even though they do not appear to have been directly influenced by the Brazilians, as the author freely admits (p. 10), there were other ways they could have learned about this fascinating movement. Indeed, although the Brazilians wrote in Portuguese rather than Spanish, they were famous throughout South America. Via a series of detailed analyses, Robinson convincingly demonstrates that the Chilean poets employ a number of concrete principles such as lexical isolation, spatial distancing and syntactical dislocation. Reflecting life in an oppressive society, she concludes, their poetry deserves to be much better known. Concrete poetry itself, to be sure, is an offshoot of the current worldwide interest in visual poetry, which can be traced back to Stéphane Mallarmé’s Un Coup de dés n’abolira jamais le hasard (1898). Thirty-six years ago, unaware that visual poetry would soon encompass exciting new versions, I ventured to describe it as ‘poetry that is meant to be seen’ (The Aesthetics of Visual Poetry, 1914–1928 (1986), p. 2). Robinson proposes a modified version of this definition: visual poetry is ‘poetry that is made to be seen’ (p. 16). This reformulation nicely captures the concretist aesthetic, which she examines at considerable length. Since concrete poets treat the word on the page (or elsewhere) as an isolated construct with no history or origin, they force the reader/viewer to consider the written word solely as an object. Thus, as Robinson skilfully demonstrates, the three Chilean poets ‘emphasize the materiality of language, dismantle linear narrative, confuse traditional verbal and pictorial conventions, and engage in a meta-discourse on art’ (p. 13). Exactly how they implement this ambitious program is the subject of the book. Ignoring the concretist credo that critical analysis should originate in a vacuum, Robinson provides numerous biographical details that expand our understanding of the poetry.

The Korean War Identification Project: 30 years of expanding scope and complexity in the accounting of American war dead

AbstractThe Korean War claimed approximately 36,500 American service member casualties in its 3 years, of which nearly 7600 are still unaccounted for. The Korea 208 (K208) Project began in 2011 to more strategically address the highly commingled nature of 208 boxes of human remains that were repatriated from North Korea to the United Nations Command in the early 1990s and subsequently turned over to the Central Identification Laboratory, Hawaii (now Defense POW/MIA Accounting Agency [DPAA]) for analysis and identification. The K208 was further expanded by the recovery of remains from Joint Recovery Operations (JROs) in North Korea between 1996 and 2005, which revealed, among other issues, the presence of non‐U.S. casualties and crossover of elements between these two assemblages. More recently, 55 boxes of human remains (known as the K55) were repatriated from North Korea in 2018 with many of the same commingling and assemblage crossover issues originally presented by the K208. Additionally, there has been an increased push to disinter more than 800 unknown Korean War service members buried at the National Memorial Cemetery of the Pacific (also known as the Punchbowl) in Honolulu, which represent casualties recovered from both North and South Korea that had undergone prior analysis in the 1950s and 1960s. Subsequent examination has revealed that many Punchbowl remains are commingled despite being thought to have been originally buried as discrete individuals. Because of these developments, the K208 Project has grown in complexity and scope, thus changing its name to the Korean War Identification Project (KWIP) in 2017. Now under its purview are the original accessions from the K208 and JROs as well as the K55 and Punchbowl, representing the largest identification project within the DPAA. A robust and defensible identification process begins with an initial triage of the remains, followed by a systematic process of segregation, consolidation, and corroboration leading to identification. This unique case of highly commingled human remains, involving thousands of missing persons from varied proveniences, highlights the challenges and successes of a multi‐disciplinary approach involving historians, anthropologists, odontologists, and DNA and isotope specialists at the forefront, while also working with family members towards resolution. As of December 31, 2020, the KWIP has identified a total of 591 service members and continues to seek ways to innovate and improve both the quantity and quality of its work.This article is categorized under: Forensic Anthropology > Anthropology in Mass Disaster & War Crime Contexts Forensic Biology > Forensic DNA Technologies Forensic Medicine > Historical Perspectives

1046. SORBUS CAMBRENSIS: Rosaceae

SummarySorbus cambrensis, WELSH WHITEBEAM, is described and illustrated. It is a member of Sorbus subgenus Aria and is unusual in being a pentaploid. It is endemic to six sites in the eastern Brecon Beacons, Wales, and is ‘Endangered.’ It was first noted as a ‘large‐leaved S. porrigentiformis’ in the 1970s and was described in 2009. It is closely related to another pentaploid, S. stenophylla, both of which may be derived from hybridisation between S. rupicola and unknown members of Sorbus subgenus Aria.

Hereditary hematologic malignancies: a Canadian perspective

When a patient is newly diagnosed with a malignancy, two common questions are often asked: 1) why did I get this cancer and 2) are my children or other family members at risk? In the case of hematologic malignancies, the standard response has been that the cause is unknown and family members are not at increased risk. However, hereditary predisposition to hematologic malignancies, especially myeloid malignancies, is becoming increasingly recognized, necessitating a change to this dogma. Hereditary hematologic malignancies are not as rare as previously believed, with an ever-increasing number of predisposition genes and alleles being discovered. Since the initial discovery of familial platelet disorder with associated myeloid malignancy (FPDMM) due to deleterious germline variants in RUNX1 in 1999, the list of predisposition genes, such as CEBPA, DDX41, ETV6, GATA2, and others continues to grow.

Coupling methanotrophic denitrification to anammox in a moving bed biofilm reactor for nitrogen removal under hypoxic conditions

Simultaneous removal of ammonium and nitrate was achieved in a methane-fed moving bed biofilm reactor (MBBR). In the reactor, methanotrophic microorganisms oxidized methane under hypoxic conditions likely to methanol, hence providing an electron donor to denitrifiers to reduce nitrate to nitrite that then allowed anaerobic ammonium oxidizing bacteria (Anammox) to remove excess ammonium as N2. The ammonium and nitrate removal rates reached 72.09 ± 5.81 mgNH4+–N/L/d and 62.61 ± 4.17 mgNO3−–N/L/d when the MBBR was operated in continuous mode. Nitrate removal by the methane-fed mixed consortia was confirmed in a batch test revealing a CH4/NO3− molar removal ratio of 1.15. The functional populations were unveiled by FISH analysis and 16S rRNA gene sequencing, which showed that the biofilm was dominated by Anammox bacteria (Candidatus Kuenenia) and diverse taxa associated with the capacity for denitrification: aerobic methanotrophs (Methylobacter, Methylomonas, and unclassified Methylococcaceae), methylotrophic denitrifiers (Opitutaceae and Methylophilaceae), and other heterotrophic denitrifiers (Ignavibacteriaceae, Anaerolineaceae, Comamonadaceae, Rhodocyclaceae and Thauera). Neither DAMO archaea nor DAMO bacteria were found in the sequencing analysis, indicating that more unknown community members possess the metabolic capacity of methanotrophic denitrification.

The power of the Ingroup for promoting collective action: How distinctive treatment from fellow minority members motivates collective action

Around the world, protests tied to the Black Lives Matter movement are highlighting myriad forms of unjust treatment that racial and ethnic minorities face, and prompting countries to reckon with these injustices. When considering racial/ethnic minorities' motivation to engage in these collective actions (alongside allies), it is certainly spurred in part by witnessing and experiencing such unjust treatment. Yet because this intergroup mistreatment commands strong attention (rightly so), less attention has been given to another potential force behind minorities' collective action motivations – the (positive) treatment coming from members of their own racial/ethnic group. Bridging theory on intragroup relations and collective action, in four studies we demonstrate that when racial/ethnic minorities are shown appreciation for the ideas and insights they bring to their group – for instance, when fellow members seek them out for their ideas during conversation; expressions of distinctive treatment – it positively affects their sense of value to the group as a whole, and, in turn, their motivation to engage in collective action. Moreover, we demonstrate how these processes feed into other established explanations for collective action, outlined in the social identity model of collective action (SIMCA; e.g., perceived injustice). We also show that even a single expression of distinctive treatment from a few unknown ingroup members can have positive effects, especially when those members have high standing within the group. Overall, this illustrates the power of the ingroup – how taking opportunities to seek out a fellow member's ideas and perspectives can be a potent force for promoting collective action.

Review of optically active and nonlinear chiral metamaterials

Advanced photonic nanostructures have enabled the maximization of synthetic chiroptic activities. The unique structuring of these building blocks has empowered chiral selective interactions with electromagnetic waves in plasmonic structures and dielectric media. Given the repertoire of optimized chiral surfaces in the literature and the ubiquity of chirality in the organic realm, the natural direction to consider is the operation of these devices in larger optical systems much like their chiral organic counterparts. In this review, we recapitulate advances in active and nonlinear chiral metamaterials. Many of the results, such as the magneto-chiral anisotropy and third-harmonic Rayleigh scattering optical activity, are relatively unknown members of the more conventional family of tuning methodology and nonlinear processes. We believe they are poised to play an instrumental role in designing advanced chiroptic systems for applications in biochemistry, valleytronics, spintronics, and chiral quantum optics.

Tap water as a natural vehicle for microorganisms shaping the human gut microbiome.

Fresh potable water is an indispensable drink which humans consume daily in substantial amounts. Nonetheless, very little is known about the composition of the microbial community inhabiting drinking water or its impact on our gut microbiota. In the current study, an exhaustive shotgun metagenomics analysis of the tap water microbiome highlighted the occurrence of a highly genetic biodiversity of the microbial communities residing in fresh water and the existence of a conserved core tap water microbiota largely represented by novel microbial species, representing microbial dark matter. Furthermore, genome reconstruction of this microbial dark matter from water samples unveiled homologous sequences present in the faecal microbiome of humans from various geographical locations. Accordingly, investigation of the faecal microbiota content of a subject that daily consumed tap water for 3 years provides proof for horizontal transmission and colonization of water bacteria in the human gut.

Mining folded proteomes in the era of accurate structure prediction.

Protein structure fundamentally underpins the function and processes of numerous biological systems. Fold recognition algorithms offer a sensitive and robust tool to detect structural, and thereby functional, similarities between distantly related homologs. In the era of accurate structure prediction owing to advances in machine learning techniques and a wealth of experimentally determined structures, previously curated sequence databases have become a rich source of biological information. Here, we use bioinformatic fold recognition algorithms to scan the entire AlphaFold structure database to identify novel protein family members, infer function and group predicted protein structures. As an example of the utility of this approach, we identify novel, previously unknown members of various pore-forming protein families, including MACPFs, GSDMs and aerolysin-like proteins.

Ancient DNA Methods Improve Forensic DNA Profiling of Korean War and World War II Unknowns.

The integration of massively parallel sequencing (MPS) technology into forensic casework has been of particular benefit to the identification of unknown military service members. However, highly degraded or chemically treated skeletal remains often fail to provide usable DNA profiles, even with sensitive mitochondrial (mt) DNA capture and MPS methods. In parallel, the ancient DNA field has developed workflows specifically for degraded DNA, resulting in the successful recovery of nuclear DNA and mtDNA from skeletal remains as well as sediment over 100,000 years old. In this study we use a set of disinterred skeletal remains from the Korean War and World War II to test if ancient DNA extraction and library preparation methods improve forensic DNA profiling. We identified an ancient DNA extraction protocol that resulted in the recovery of significantly more human mtDNA fragments than protocols previously used in casework. In addition, utilizing single-stranded rather than double-stranded library preparation resulted in increased attainment of reportable mtDNA profiles. This study emphasizes that the combination of ancient DNA extraction and library preparation methods evaluated here increases the success rate of DNA profiling, and likelihood of identifying historical remains.

Metagenomic screening strategies for bioprospecting enzymes from environmental samples

Globally, there is a growing demand for biocatalysts because of the associated efficacy and efficiency. The applications of enzymes in food, paper, pulp, textile, and chemical industries have prompted enzyme exploration. Microbes, being the natural reservoirs of enzymes, have gained researchers’ attention, and the quest for microbial enzymes has increased in past years. This review provides insights about metagenomics techniques and their applicability in obtaining microbial-origin enzymes from diverse environmental samples besides highlighting their importance. The metagenomic approach has emerged as a promising way towards replacing conventional microbial techniques with culture-independent methods involving direct isolation of DNA environmental samples. There are two primary methodologies, i.e., functional-based and sequence-based, to identify and characterize industrially valuable biocatalysts from the environmental microcosms. Many of the obtained enzymes are successfully used in diverse food, cosmetics, and pharmaceutical industries. However, there are some complications associated with it that can be minimized only by further investigations. The paper focuses on the advancement of metagenomics for bioprospecting to stress on its significance in microbial characterization and exploration. This will also ensure the in-depth analysis of several unexplored and unknown microbial communities and/or members from complex niches.

Little-Known Pages of Ledokhovskhy Family’s Life. Architect Ignatiy Ledokhovskhy: Creativity and Fate

Ignacy-Władysław Ledóchowski worked in Kyiv in the first quarter of the twentieth century. He left the citizens of Kyiv unique buildings in the Art Nouveau style, which has become the decoration of the city for the last few centuries. Based on the analysis of newly discovered sources, the article highlights the little-known pages of the history of the Polish szlachta Ledokhovsky family (Szaława coat of arms) and presents biographies of Kyiv architect Ignatiy Ledokhovsky (1867–1932) and his family; brothers Karol (1871–1931) and Stanislaw (1874–1940). The scientific novelty of this article is highlighted by revealing new information relating to the biography of the architect Ledokhovsky and his family. It was discovered that the future architect Ignacy-Władysław Ledóchowski was born in the village of Strilnyki (pol. Strzelniki), Vinnytsia region, in 13 in old style (25 new style) May 1867, and died on the night of March 2 to 3 1932, in Warsaw, where he was buried. His parents were Kazimir, son of Vladyslav and Jozefa nee Danielevich, Ledokhovsky. The article traces the biographies of his two younger brothers Karol and Stanislav, clarifies the history of the estates of this branch of the Ledokhovsky family in Podillya, in the Right-bank Ukraine. This research on the life and work of the architect Ledokhovski will reveal unknown until now details of his biography, increased materials on his life and work in Ukraine and Poland, as well as some practically unknown members of the Ledokhovsky family.

Conservation Treatment on the Bamboo Sunblind from the No. 1 Catchment Site in Baesanseongji, Busan

In the present study, safe management and value improvement of bamboo sunblind, which is an item of cultural heritage, were performed by adopting stable conservation treatment methods. The bamboo sunblind used in the present study was excavated from No. 1 catchment site in Baesanseongji, Busan. It was determined that the main material used to make the sunblind was bamboo, and herbal plants were used to weave the bamboo using lacquer as an adhesive agent. All contaminants and soil adhered to the sunblind was removed. Thereafter, the sunblind, which was recovered in the form of blocks, was washed separately after fixing it to a temporary plaster frame and to avoid the blocks from breaking during washing. Then, polyethylene glycol (PEG) impregnation was utilized for the reinforcement treatment. Based on the preliminary test results, the shape of the sunblind was fixed using a stainless-steel frame to prevent physical damage that may occur during the drying process. Thereafter, the bamboo sunblind was vacuum freeze-dried. PEG 20% (in ethyl alcohol) was applied as a surface treatment agent for stabilization the sunblind. After the surface treatment, the bamboo sunblind were joined together to fit the maximum width, and the rectangular shape of the sunblind was restored—as best as possible—while filling in the missing parts by maximizing the use of unknown members such as in the disturbed layers below bamboo sunblind surface. The conservation treatment was completed by fixing the bamboo sunblind into the fabricated frame.