Universidade Federal De Research Articles

First report of Kosakonia cowanii causing bacterial blight on Coffea arabica.

From 2012 to 2019, 6-month to 2-year-old coffee (Coffea arabica) plants showing leaf blight, stem blackening, brownish, necrotic, irregular leaf spots and shoot tip dieback (Figure 1A, B, C, and D) symptoms were observed at the municipalities of Araguari, Indianópolis, Monte Carmelo, Nova Ponte and Romaria, Minas Gerais State, Brazil. Bacterial exudates from infected tissues, mainly from the shoot tip, were observed under microscopy; no bacterial exudates were found in leaf tissues. Bacterial colonies were isolated from symptomatic disinfested tissues (leaves and shoot tip) using medium 523 (Figure 1E). The isolated bacteria were facultative anaerobe, Gram-negative, cream-colored on YDC medium, growth at 37 oC, arginine and oxidase negative, catalase and asparagine positive (Schaad et al. 2002), and positive hypersensitivity reaction in tobacco leaves. Pathogenicity was confirmed by spraying coffee plants (at the three-leaf stage) until runoff, with a 1 × 108 CFU/mL bacterial suspension inoculate. The plants were kept in the moist chamber for 24 hours, before and after inoculation under greenhouse conditions. No symptoms were observed under the mock-inoculated plants. Disease symptoms on inoculated plants were observed 13 days after inoculation. The bacteria were then reisolated to satisfy Koch's postulates, the colony phenotypes were identical to the original ones. Bacterial genomic DNA of four isolates (UFU D1, UFU G119, UFU H1, and UFU I87) was extracted, and the 16S rRNA gene region was amplified using the 8F and 1492R universal primers, and then compared with sequences deposited in the GenBank. The results aligned closely with those of Kosakonia cowanii (GenBank NR_025566.1), with 99.4% similarity and 96% query coverage for the sequence. In addition to sequencing the 16S rRNA gene, which is considered the barcode for bacteria, other single-copy genes are necessary for species confirmation. In this study, to confirm the species and generate more data on these new isolates, 150 bp paired-end libraries were constructed and sequenced using DNBSEQ. A minimum of 1 gigabase was obtained for each isolate, and the genomes were assembled using the SPAdes genome assembler v3.15.4. Subsequently, the in-house developed algorithm SpM (Species Matching) was used to identify the species. The phylogenetic tree for the 16S gene was constructed using the maximum likelihood algorithm available in PhyML (v3.1/3.0 aLRT). The Phylogeny.fr platform was used to perform the phylogenetic reconstruction. This analysis revealed that our K. cowanii isolates (H1, D1, G119, and I87) from the coffee tree clustered with other K. cowanii strains isolated from different locations (Figure 2A). The Average Nucleotide Identity (ANI) analysis was performed using the fastANI program on all complete genomes of the Kosakonia genus obtained from the NCBI database. The results indicated that our K. cowanii isolates from the coffee tree clustered closely with other strains of the K. cowanii species (Figure 2B). Genomic analyses confirmed that all isolates belong to the species K. cowanii, corroborating the 16S rRNA gene analyses. The genomes have been deposited in NCBI with the accession numbers CP160410 (K. cowanii strain UFU H1, size 4,749,805 bp and coverage 36.21x), CP160412 (strain UFU G119, size 4,509,735 bp and coverage 31.26x), CP162577 (strain UFU D1, size 4,867,107 bp and coverage 19.0x), and CP162576 (strain UFU I87, size 4,552,151 bp and coverage 18.23x). These results are of great importance for better understanding the role of K. cowanii as a disease-causing pathogen of coffee plants. K. cowanii has already been described infecting Eucalyptus with symptoms of bacterial blight in Uruguay (Brady et al. 2009), causing disease in soybeans (Krawczyk and Borodynko-Filas 2020), also associated as endophytic in plants (Thomas et al. 2007), and as human pathogens (Yang et al. 2018). Thus, to our knowledge, this is the first report of bacterial blight caused by K. cowanii on coffee plants. The isolates are deposited in the phytopathogenic bacteria collection at Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Brazil, under the codes UFU D1, UFU G119, UFU H1 and UFU I87.

The dung beetles of Venezuela (Coleoptera: Scarabaeidae: Scarabaeinae): catalogue and updated distribution

Venezuela’s diverse land ecosystems are grouped into four major regions (coast-islands, low plains, hills and mountains), ranging from sea level up to 4978 m. The Scarabaeinae (Scarabaeidae, Coleoptera) currently encompass 278 genera and 6837 species worldwide, but are poorly inventoried in Venezuela. We reviewed the literature and the material housed at the entomological collection of the Universidade Federal de Mato Grosso (CEMT), Cuiabá, Brazil, and found 32 genera and 149 species of dung beetles as certainly present in Venezuela. Twenty-four of these species are, as far as current knowledge goes, endemic to the country, while another 34 are restricted to Venezuela and the neighbouring countries of Colombia, Brazil, Trinidad and Tobago, and Guyana. Additionally, 36 species are deemed potential inhabitants of the country, whilst 14 others previously recorded in the literature as part of the Venezuelan fauna are here concluded not to be actually present there. Complete literature is listed for each genus and species, and information on type material, material examined, worldwide distribution, and Venezuelan records is also presented.

The Passo das Tropas outcrop complex and the Dicroidium Flora in southern Brazil

The Dicroidium flora, composed of different genera of fossil plants, has its first records dating back to the Permian, expanded significantly in diversity in the Triassic, occupying several regions of the Gondwanan territory, becoming a biostratigraphic landmark for the Triassic. In Brazil, this paleoflora is found in the sedimentary rocks of the Santa Maria region, state of Rio Grande do Sul, in locations close to the Passo das Tropas Creek, an informal type section of the Passo das Tropas Member, of the Santa Maria Formation. Pioneering studies on the subject in the region began in 1952, at the Passo das Tropas outcrop, which described the first records of the Dicroidium flora. Over the years, different outcrops were found in this region, which also revealed this paleoflora, such as: “Dom Antônio Reis” outcrop, “Zenir Aita” outcrop and “Espuma” outcrop, where several paleontological collections were carried out. In addition to plant fossils, fish scales and insect wings were recorded in these outcrops. However, these outcrops were totally or partially buried due to the urbanization process in the city of Santa Maria and its surroundings, making further studies impossible. This article presents a historical and stratigraphic review, compiling the work carried out in the last 70 years on the outcrops of the Passo das Tropas Member and presents a new fossil site, the outcrop called Estância dos Montes. A total of 125 specimens were recovered from the Estância dos Montes outcrop, belonging to Dicroidium odontopteroides, D. zuberi, D. lancifolium, Umkomasia sp., Pteruchus sp., Neocalamites sp. and seeds. The fossils presented in this work come from a paleontological rescue carried out at the Estância dos Montes outcrop and are currently deposited in the Paleontological Collection of the Laboratório de Estratigrafia e Paleobiologia of the Universidade Federal de Santa Maria. Not only the importance of preserving and protecting fossil sites and their contents is highlighted, but also the uniqueness of these fossils for the valorization of the regional heritage of Santa Maria and the global relevance of adding more data to understand the biota during the Gondwanan Triassic.

Aspergillus capibaribensis (Eurotiales, Trichocomaceae), a new species in section Cremei

In this study we describe a new species of Aspergillus in section Cremei deposited in the URM culture collection (Micoteca URM Profa. Maria Auxiliadora Cavalcanti) of the Departamento de Micologia, Universidade Federal de Pernambuco (Recife, Pernambuco, Brazil). Another strain was obtained from samples taken from pollen pots inside nests of Melipona scutellaris (stingless bee). Morphological and phylogenetic evidence based on sequencing of ITS rDNA, BenA, CaM and rpb2 markers supported the description of taxonomic novelty. Aspergillus capibaribensis sp. nov. is morphologically similar and phylogenetically close to A. flaschentraegeri - the distinctive features are uniseriate and hyaline conidiophores, short and smooth conidia and smaller vesicles compared to the micromorphological structures of A. flaschentraegeri. This record also expanded the knowledge of species in Aspergillus section Cremei in a tropical country such as Brazil.

Machine Learning Approach to Support Taxonomic Discrimination of Mayflies Species Based on Morphologic Data.

Artificial intelligence (AI) and machine learning (ML) offer objective solutions in the elaboration of taxonomic keys, such as the processing of large numbers of samples, aiding in the species identification, and optimizing the time required for this process. We utilized ML to study the morphological data of eight species of Americabaetis Kluge 1992, a diverse genus in South American freshwater environments. Decision trees were employed, examining specimens from the Museu de Entomologia da Universidade Federal de Viçosa (UFVB/Brazil) and literature data. Eleven morphological traits of taxonomic importance from the literature, including frontal keel, shape of the mouthparts, and abdominal color pattern, were analyzed. The decision tree obtained with the Gini algorithm effectively differentiates eight species (40% of the known species), using only eight morphological characters. Our analysis revealed distinct groups within Americabaetis alphus Lugo-Ortiz and McCafferty 1996a, based on variations in abdominal tracheae pigmentation. This study introduces a novel approach, integrating AI techniques, biological collections, and literature data for aid in the Americabaetis species identification. It provides a valuable tool for taxonomic research on contemporary and extinct mayflies.

Ataxias in Brazil: 17 years of experience in an ataxia center.

Cerebellar ataxias comprise sporadic and genetic etiologies. Ataxia may also be a presenting feature in hereditary spastic paraplegias (HSPs). To report a descriptive analysis of the frequency of different forms of cerebellar ataxia evaluated over 17 years in the Ataxia Unit of Universidade Federal de São Paulo, Brazil. Charts of patients who were being followed from January 2007 to December 2023 were reviewed. We used descriptive statistics to present our results as frequencies and percentages of the overall analysis. Diagnosed patients were classified according to the following 9 groups: sporadic ataxia, spinocerebellar ataxias (SCAs), other autosomal dominant cerebellar ataxias, autosomal recessive cerebellar ataxias (ARCAs), mitochondrial ataxias, congenital ataxias, X-linked ataxias, HSPs, and others. There were 1,332 patients with ataxias or spastic paraplegias. Overall, 744 (55.85%) of all cases were successfully diagnosed: 101 sporadic ataxia, 326 SCAs, 20 of other autosomal dominant cerebellar ataxias, 186 ARCAs, 6 X-linked ataxias, 2 mitochondrial ataxias, 4 congenital ataxias, and 51 HSPs. This study describes the frequency of cerebellar ataxias in a large group of patients followed for the past 17 years, of whom 55% obtained a definitive clinical or molecular diagnosis. Future demographic surveys in Brazil or Latin American remain necessary.

The use of project pedagogy in teletandem sessions

This article describes an online language learning context in which teletandem sessions (Telles, 2009) have been integrated into the syllabus of a Modern Languages Degree Course at the Universidade Federal de Goiás (UFG), Brazil, as an internationalization at home action. In the second semester of 2021, the subject “Aprendizaje de Portugués y Español en Teletandem” was offered with the participation of ten Brazilian students who were majoring in Spanish at the UFG, nine Argentine students from different undergraduate courses from the Universidad de Cuyo, and one undergraduate student from the Universidad de Buenos Aires. In this virtual learning context, the Brazilians could teach Portuguese to the Argentinians and improve their fluency in Spanish through interactions with them. They also had to develop a project about a chosen theme and work on it, considering the Brazilian and Argentine contexts. The data for this case study (Johnson, 1992) was obtained through video recordings of the interviews of two pairs of students. The results show that the participants could learn an additional language, improve their intercultural skills, and talk about real-world problems in Brazil and Argentina, like the lack of water in some cities, the devaluation of women’s work, etc. The students’ reflections on these topics made them more socially aware of living in a more harmonious world where diversity is highly valued.

From laboratories to Museum: the trajectory of the mineralogy collection of the Escola de Minas de Ouro Preto

The paper presented is the result of the author's doctoral research, which included an excerpt on the formation of the mineralogy collection of the former Escola de Minas de Ouro Preto, currently the Escola de Minas da Universidade Federal de Ouro Preto. This analysis aims to map the trajectory of the collection from its entry to the museumization of the samples. As a methodological path, the exploratory research collected information in online repositories and in the archives of the Escola de Minas da UFOP and the Museu de Ciência e Técnica da Escola de Minas da UFOP. This study sought to answer how the set of minerals present in the Mineralogy Office contributed to the training of mining engineers in the 19th and early 20th centuries. The results show a movement of collections from other national and foreign institutions and their didactic use even after their museumization in 1930 1. Introdução .

Audiences of visits scheduled to the Museu de História Natural e Jardim Botânico of the Universidade Federal de Minas Gerais: contributions from records to museum management

The text presents an analysis of records of scheduling group visits to the Museu de História Natural e Jardim Botânico da Universidade Federal de Minas Gerais - Natural History Museum and Botanical Garden of the Federal University of Minas Gerais (MHNJB-UFMG) in the years 2018 and 2019. As a research source, records from the area of scheduling of the Educational Sector. From the analysis undertaken, it was possible to highlight the profile of these groups, requested educational itineraries and visit objectives. The data collected was categorized, organized, processed and interpreted. The results indicated that 86.8% of the institutions that requested the visit carried it out. The greatest demand for the MHNJB-UFMG is from schoolchildren, 86.1%, with emphasis on public education institutions and students enrolled in the initial years of Elementary School. Most scheduled groups come from the Metropolitan Region of Belo Horizonte and the most requested educational itinerary was the Circuito História Natural - Natural History Circuit. Within the scope of non-school institutions, the private sector segment was the one that most attended. School visits are concentrated from Tuesdays to Fridays. The data collected is useful for the management of the MHNJB-UFMG, as it makes it possible to review registration processes and highlight possible demands to propose actions aimed at these visitors, after knowing their motivations and expectations when making the appointment.

From laboratories to Museum: the trajectory of the mineralogy collection of the Escola de Minas de Ouro Preto

The paper presented is the result of the author's doctoral research, which included an excerpt on the formation of the mineralogy collection of the former Escola de Minas de Ouro Preto, currently the Escola de Minas da Universidade Federal de Ouro Preto. This analysis aims to map the trajectory of the collection from its entry to the museumization of the samples. As a methodological path, the exploratory research collected information in online repositories and in the archives of the Escola de Minas da UFOP and the Museu de Ciência e Técnica da Escola de Minas da UFOP. This study sought to answer how the set of minerals present in the Mineralogy Office contributed to the training of mining engineers in the 19th and early 20th centuries. The results show a movement of collections from other national and foreign institutions and their didactic use even after their museumization in 1930.

The education of fishing workers about accidents caused by marine animals: a study in the Região dos Lagos of Rio de Janeiro

Fishing exposes workers to various occupational risks, including accidents caused by poisonous, venomous and traumatizing marine animals. With this in mind, the aim of this study is to present educational activities for people working in the fishing activities in the Região dos Lagos (Lakes Region) of Rio de Janeiro, southeastern Brazil, with a focus on accidents caused by marine animals (ACMA). The protocol was approved by the Human Research Ethics Committee of the Universidade Federal de Viçosa (CAAE: 53959421.8.0000.5153). The activities were carried out in the municipalities of Armação dos Búzios, Arraial do Cabo and Cabo Frio and included a workshop on the subject, lasting an average of four hours, preceded (pre-test) and followed (post-test) by an assessment consisting of objective questions on the ACMA. A total of 47 workers (with an average working time of 15.2±12.8 years) took part in the activity. Each professional suffered an average of three ACMA. A comparison of the pre-test and post-test results showed a higher percentage of correct answers to almost all the questions. Further studies are needed to estimate the risks of ACMA among fishing workers and to improve educational actions to minimize the impact of these injuries on this population.

Photobiomodulation therapy for chronic knee pain in obese patients in pre-rehabilitation for bariatric surgery: randomised, placebo-controlled, double-blinded, clinical trial protocol

IntroductionObesity has become a worldwide public health problem and is directly linked to loss of quality of life, complications and comorbidities. One of them is chronic pain, especially in the...

Editorial

The Latin American Journal of European Studies (ISSN 2763-8685) is a publication of the Latin American Center of European Studies, currently supported by the Jean Monnet Network Policy Debate “BRIDGE Watch” project with funding from the Erasmus + Programme of the European Commission. With a focus on the editorial lines on European Union Law and Policies and International Relations of the European Union with third countries and Latin America, the seventh edition of the Journal also has a thematic dossier on “Rule of Law in Latin America and Europe” organized by Aline Beltrame de Moura of the Universidade Federal de Santa Catarina (Brazil). The seventh edition of the Journal is open for submissions until May 1st, 2024.

The Axiidea in the Museum of Oceanography Petrônio Alves Coelho, Recife, Brazil, with Some Remarks on the Biology of the Species

Axiidea housed in the collection of the Museu de Oceanografia Prof. Petrônio Alves Coelho, Universidade Federal de Pernambuco (MOUFPE) were studied. This collection contains 66% of the total diversity of axiideans recorded from the continental shelf of the Brazilian coast. The species are listed by family and genus as follows: Axiidea: Axiidae (Axiopsis, Axiorygma, Calaxius, Coralaxius, Manaxius, Paraxiopsis), Callianassidae (Cheramoides), Callichiridae (Callichirus, Corallianassa, Lepidophthalmus, Neocallichirus), Ctenochelidae (Ctenocheles, Ctenocheloides, Dawsonius, Gourretia), Micheleidae (Marcusiaxius, Meticonaxius). Details on the biology and taxonomy of some of these species are given. In addition, certain poorly documented species are now better understood.

Economic analysis of banana peel and sweet potato vines in diets for rabbits.

This study aimed to evaluate the costs of using banana peel (BP) and sweet potato vine (SPV) in rabbit diets. BP and SPV were chosen to replace maize and alfalfa hay, because, in addition to the ingredients having similar nutritional characteristics, they are among the most expensive ingredients in rabbit diets. Data were obtained through a biological assay carried out in the Cuniculture Laboratory of the Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Fifty New Zealand white rabbits, weaned at 35days of age, were fed during the growth phase (35 to 84days) with increasing levels of BP and SPV, replacing maize and alfalfa hay (T0, T25, T50, T75 and T100% replacement). A diet containing 100% replacement (T100) had the lowest cost per kilogram, which was R$ 1.18/kg, while the controlled diet was 57% more expensive, costing R$ 2.08/kg. The total operating cost to produce a rabbit with control treatment was R$10.93/head and at T100, it was R$6.51/head. The animal income for a live rabbit was R$ 24.08 and R$ 23.95 in treatments T0 and T100, respectively. Regarding the gain margin per animal (GMA), in TO it was R$ 13.16 while in T100 it was R$ 17.44, therefore the GMA was 32.55% higher when using the T100 diet. In conclusion, it is more economical and feasible to feed rabbits with diets containing 100% BP and SPV, replacing maize and alfalfa hay.

Aspectos bioecológicos de Cynea (Cynea) diluta (Herrich-Schäffer, 1869) (Lepidoptera: Hesperiidae: Hesperiinae)

In Hesperiidae (Lepidoptera), many species build shelters during their ontogeny which may be serve as a scape strategy to reduce attacks from natural enemies and dislodgment from the host plant. This paper provides bioecological information regarding host plant, natural parasitism, and distribution of Cynea (Cynea) diluta (Herrich-Schäffer) (Hesperiidae: Hesperiinae) collected near remnants of the Atlantic Forest “Centro de Endemismo Pernambuco” (CEPE), Northeast Region of Brazil. Larvae and pupae, the later showing signs of parasitism, were collected from a garden at the Universidade Federal de Alagoas, Maceió, State of Alagoas, Brazil. They were brought to the laboratory to record emergences and possible parasitoids. Data from distribution were obtained from labels of hesperiid deposited from the Coleção Entomológica Padre Jesus Santiago Moure,Curitiba, State of Paraná. The host plant was identified as Alpinia purpurata (Vieill.) K. Schum. (Zingiberaceae). From the 13 larvae and two pupae collected in the environment, 20 specimens of Brachymeria sp. (Hymenoptera: Chalcididae) emerged from the pupae. This is the first report of parasitism on pupae of Cynea (C.) diluta.

Azospirilla and Nitrogen Fertilization to Promote Maize Seed Development and Yield

Aims: This study evaluated the interaction of Azospirillum brasilense (Ab) strains and nitrogen fertilizer doses on maize.
 Study Design: Laboratory experiment evaluated Ab strains (PCR and PTF) on maize seed germination and vigor (completely randomized design). Field experiment evaluated Ab strains and nitrogen fertilization on maize yield parameters (randomized block design).
 Place and Duration of Study: The germination and vigor experiment (10 days in April 2019) was implemented in a controlled environment at the Seed Analysis Laboratory of the Universidade Federal de Uberlândia, Brazil. The field experiment (137 days in winter 2019) was implemented at the Fisioplant Research Station (Uberlândia, Brazil) under commercial maize crop management.
 Methodology: In controlled conditions (laboratory), 200 seeds of maize (K9105VIP3, Riber KWS Seeds) were incubated (25 °C) with the Ab strain solutions for germination and vigor evaluations. In the field, 60,000 plants ha-1 (0.5 m space between sowing lines) of maize were cropped under combinations of nitrogen fertilizer and volumes of Ab strains (treatments) for leaf nitrogen, SPAD index, leaf count, fresh and dry maize shoot biomass, one-thousand grains weight, grain rows per maize ear, productivity (kg ha-1).
 Results: Treatment with 100 mL ha-1 of Ab PFT strain increased maize seed germination and vigor more than Ab PCR strain and control (no-inoculation or fertilizer). The number of grain rows of the 100% N + 150 mL ha-1 of Ab PFT strain and the maize shoot dry mass of the 100% N (no-inoculation) were higher than the control. The treatments did not affect the SPAD index, weight of one thousand grains, fresh maize shoot biomass, or productivity.
 Conclusion: Using Ab with nitrogen fertilizer is a promising strategy to improve maize crop responses; however, more studies are needed to detail the ideal management of Ab strains for maize crops and yield construction.

Overview of Fourth Generation Reactors Studied at the Universidade Federal de Minas Gerais (Brazil)

This paper presents the results of the studies about the Fourth Generation Reactors developed at DENU-UFMG, such as the Very High Temperature Reactor (VHTR), Molten Salt Breeder Reactor (MSBR), Sodium Fast Reactor (SFR) and Gas-cooled Fast Reactor (GFR). These studies evaluate neutronic parameters of the nuclear system at steady state and during the burnup using computer models developed at DENU-UFMG using SCALE 6.0 (KENO-VI/ORIGENS), MCNPX 2.6.0 and MCNP5. The results show that the adopted methodologies confirm the capabilities of the codes to simulate specific situations in steady state or transient operating conditions.

OSMOPRIMING AND ITS RELATIONSHIP WITH THE QUALITY OF Urochloa decumbens SEEDS UNDER WATER DEFICIT

The use of high-quality Urochloa decumbens seeds is an essential factor for the establishment of pastures, and osmopriming under stress conditions may improve seed performance. This study was conducted at the Universidade Federal de Viçosa in a completely randomized design and aimed to evaluate the effects of osmopriming and its relationship with physical and physiological attributes of U. decumbens seeds subjected to water deficit. Seeds from ten lots were primed in H2O (0 MPa) and PEG (−0.8 MPa), KNO3 (0.2%), and SNP (0.10 mmol L−1) solutions for 24 hours. Unprimed seeds were used as control. The seeds were evaluated in two trials after osmopriming. Trial I consisted of the X-ray test to evaluate physical attributes and germination and vigor tests. In Trial II, the seeds were placed to germinate under favorable (0 MPa) and water deficit conditions (−0.2 MPa) to evaluate physiological attributes. In general, osmopriming of U. decumbens seeds assisted in increasing physical attributes such as area, gray scale, perimeter, and tissue density. Osmopriming does not contribute to the best physiological performance of U. decumbens seeds under ideal germination conditions (0 MPa). However, osmopriming with SNP (0.10 mmol L−1) and PEG (−0.8 MPa) for 24 hours contributes to the better physiological performance of seeds under water deficit.

A retrospective cohort study of pulp prognosis in luxated permanent teeth: a competing risk analysis.

The present retrospective cohort study aimed to evaluate luxated permanent teeth for pulp prognosis and risk factors. Case records and radiographs of 224 patients, involving 427 luxated permanent teeth, referred for treatment at the Dental Trauma Center-School of Dentistry, Universidade Federal de Minas Gerais, (DTC-SD-UFMG) from 2014 to 2022, were assessed for pulp prognosis classified as vitality, necrosis and pulp canal obliteration (PCO). A competing risk survival analysis estimated the hazards of the three outcomes, and the effect of demographic, clinical and treatment variables was tested using a cause specific Cox regression model. Pulp vitality was found in163 teeth (38.2%), pulp necrosis in 120 teeth (28.1%) and 55 teeth (12.9%) developed PCO. Pulp vitality decreased in the presence of concomitant crown fractures (HR 0.38 95% CI [0.2-0.8] p = 0.006). The risk of pulp necrosis (HR 0.62 95% CI [0.4-0.96] p = 0.03) was lower in cases with open apices but increased with concomitant crown fractures (HR 4.0 95% CI [2.6-6.1] p = 0.001) and intrusions (HR 2.3 95% CI [1.2-4.1] p = 0.007). Lateral or extrusive luxations (HR 3.0 95% CI [1.3-6.9] p = 0.001) and open apices (HR 2.4 95% CI [1.2-4.7] p = 0.01) showed higher rates of PCO. Type of luxation, the diameter of the apical foramen, and the presence of concomitant crown fractures were main determinants of pulp prognosis after luxation injuries in permanent teeth. Pulp prognosis after tooth luxation is dependent on the presence and direction of tooth displacement together with infection control. Not applicable.