Universal Core Research Articles

The need to identify anatomy-related competencies in medical education.

A profound grasp of anatomy is indispensable for shaping competent and safe medical practitioners. This knowledge acquisition is pivotal in the early stages of medical education and remains crucial throughout clinical training. However, the evolving landscape of medical education has ushered in changes to the anatomy curriculum, marked by a reduction in contact hours and a streamlined content structure to accommodate novel subjects and teaching methodologies. This transformation has precipitated a quandary in defining the essential scope and depth of anatomical knowledge to be imparted. Traditionally, surgeons assumed the role of anatomy instructors until Flexner's recommendations catalyzed the integration of trained anatomists. Nevertheless, the varied backgrounds of anatomists and the heterogeneity in anatomy curricula across institutions have introduced potential disparities in the quality of graduates. Addressing these challenges mandates the identification of key anatomy competencies tailored for undergraduate medical students. The imperative lies in ensuring that these competencies span cognitive, psychomotor, and affective domains, offering not only comprehensiveness but also direct applicability to clinical practice. Hence, this viewpoint highlights the necessity of adopting a systematic approach that includes gathering input from various stakeholders in developing and implementing a universal anatomy core competency framework, ensuring graduates are equipped for the multifaceted demands of clinical practice. Overall, the manuscript provides a comprehensive overview of the challenges and opportunities in anatomy education, with a clear call to action for a transformative approach to meet the evolving needs of medical practice.

Highly universal sensitive star core photonic crystal fiber (S-PCF)-based sensor for both chemical and biomedical applications

Highly universal sensitive star core photonic crystal fiber (S-PCF)-based sensor for both chemical and biomedical applications

An unusually large genome from an unusually large stonefly: a chromosome-length genome assembly for the giant salmonfly, Pteronarcys californica (Plecoptera: Pteronarcyidae).

Pteronarcys californica (Newport 1848) is commonly referred to as the giant salmonfly and is the largest species of stonefly (Insecta: Plecoptera) in the western United States. Historically, it was widespread and abundant in western rivers, but populations have experienced a substantial decline in the past few decades, becoming locally extirpated in numerous rivers in Utah, Colorado, and Montana. Although previous research has explored the ecological variables conducive to the survivability of populations of the giant salmonfly, a lack of genomic resources hampers exploration of how genetic variation is spread across extant populations. To accelerate research on this imperiled species, we present a de novo chromosomal-length genome assembly of P. californica generated from PacBio HiFi sequencing and Hi-C chromosome conformation capture. Our assembly includes 14 predicted pseudo chromosomes and 98.8% of Insecta universal core orthologs. At 2.40 gigabases, the P. californica assembly is the largest of available stonefly assemblies, highlighting at least 9.5-fold variation in assembly size across the order. Repetitive elements (REs) account for much of the genome size increase in P. californica relative to other stonefly species, with the content of Class I retroelements alone exceeding the entire assembly size of all but two other species studied. We also observed preliminary suborder-specific trends in genome size that merit testing with more robust taxon sampling.

Developing Learning Innovation of Digital Open Badge in Social Studies to Enhance Citizenship Characteristics of Secondary School Students

This study aims to synthesize theories and concepts related to educational management, personality traits, and citizenship characteristics evident in the education systems of the United Kingdom, Canada, the United States, Singapore, and Thailand; to develop an innovative model of open-badge digital social education to enhance the cultivation of citizenship characteristics among high school students in Chiang Mai; and to study the results of developing digital badge innovations to enhance the development of students’ personality traits and citizenship characteristics in the educational innovation area of Chiang Mai province. A mixed-method approach is employed in order to synthesize the universal core values that ensure societal peace within diverse communities from the curricula of the five countries. When comparing the results with the Thai context, it becomes apparent that the Thai curriculum still lacks precise definitions of “universal core values” and activities that instill these core values. Through purposive sampling, this study extracts the necessary values from the opinions of involved personnel and pilot schools in the Education Sandbox program in Chiang Mai. The results suggest that ten core values need to be addressed firmly in the Digital Open Badge learning innovation and guidelines for both in-class and out-of-class activities. The citizenship characteristics platform and guidelines in social studies are designed based on the 5Ts Action Learning model with 40 hours of observation. The outcomes demonstrate that overall satisfaction with the curriculum is high. Notable changes from interviews and observations indicate that responsibility and cooperation are the most significant changes in students’ character traits. These traits are expressed mostly at the individual level (35.02%), followed by the family level (29.39%), the community level (22.62%), and lastly, the global level (12.97%).

Engineering Modular DNA Reaction Networks for Signal Processing.

Diversified molecular information-processing methods have significant implications for nanoscale manipulation and control, monitoring and disease diagnosis of organisms, and direct intervention in biological activities. However, as an effective approach for implementing multifunctional molecular information processing, DNA reaction networks (DRNs) with numerous functionally specialized molecular structures have challenged them on scale design, leading to increased network complexity, further causing problems such as signal leakage, attenuation, and cross-talk in network reactions. Our study developed a strategy for performing various signal-processing tasks through engineering modular DRNs. This strategy is based on a universal core unit with signal selection capability, and a time-adjustable signal self-resetting module is achieved by combing the core unit and self-resetting unit, which improves the time controllability of modular DRNs. In addition, multi-input and -output signal cross-catalytic and continuously adjustable signal delay modules were realized by combining core and threshold units, providing a flexible, precise method for modular DRNs to process the signal. The strategy simplifies the design of DRNs, helps generate design ideas for large-scale integrated DRNs with multiple functions, and provides prospects in biocomputing, gene regulation, and biosensing.

Abstract PO5-16-04: Tissue-informed personalized MRD detection assay may outperform tumor-informed fixed panel strategy in Triple Negative Breast Cancer (TNBC)

Abstract Background: Minimal residual disease (MRD) detection by ctDNA analysis enables identifying patients with high recurrence risk and refining risk stratification to guide treatment selection precisely, which may improve patient survival or avoid overtreatment. We previously developed a 1021-gene fixed panel MRD detection assay, MNavigator V1, demonstrating high prognostic evaluation value in various solid tumors, including lung cancer, colorectal cancer, and esophageal carcinoma, and relevant research results have been published. Recently, the assay upgraded to MNavigator V2, a customized patient-specific panel strategy to ultra-deep sequencing, with higher clinical performance and more economical. Here, we head-to-head compared the performance of MNavigator V1 with V2, using the same collection of blood samples from TNBC patients. Methods: The retrospective study recruited 48 patients diagnosed with stage II~III TNBC with adequate samples (NCT04501523/NCT04803539). Five patients in the cohort received neoadjuvant therapy (Neoad-group), and the standard treatment regimen of the remaining 43 patients was adjuvant therapy (ad-group) after surgery. The surgical tumor tissue of all these patients was collected. Peripheral blood samples were collected at various time points, baseline samples before neoadjuvant therapy, 7-day after neoadjuvant therapy, 1-month postoperative, 7-day after adjuvant therapy, and subsequent every 3~6 months follow-up. The personalized, tumor-informed MRD detection approach MNavigator V2 started by identifying somatic mutation of the resected tumor with a 1021-gene panel covering 1.6Mb of the human genome. Then, a customized panel consisting of up to 20 top-ranked patient-specific somatic variants was designed. After that, unique molecular identifiers (UMI) based 100,000X ultra-deep next-generation sequencing on plasma samples was conducted by adopting the mix panel of the patient-specific panel and a 5kb breast cancer universal core panel. MNavigator V1 assay, a fixed panel assay also applied the same off-the-shelf 1021-genes panel with a sequencing depth of 10,000X to detect MRD of blood samples. Results: Until May 2023, the median follow-up time of 660 days, and 8 patients were recurrence confirmed by CT imaging. The median tracked tissue-informed mutations was 7 (2~18). In this study, the screening time point was defined as the combined MRD status of the blood samples collected before and after neoadjuvant therapy, and 1-month post-surgery for the Neoad-group, as for the ad-group, the time point referred to 1-month post-surgery and post-adjuvant therapy. If any one of these time nodes' MRD was positive, the screening period MRD was positive. During the screening period, MNavigator V1 and V2 accurately predicted 6/8 (75.0%) recurrence. MRD-positive status was associated with a worse prognosis, compared to the MRD-negative population, with hazard ratios of 9.0 and 19.9 for V1 and V2 assay, respectively. The longitudinal MRD evaluation yield a higher sensitivity of 7/8 (87.5%) for recurrence prediction with MNavigator V2 at a median lead time of 104 days, whereas the V1 assay predicted 6/8 (75.0%) at 94 days, and the hazard ratio was 40.4 vs 8.5. In one brain-only metastasis patient, MRD was undetectable, which may be due to the limitation of the blood-brain barrier. For recurrence-free patients with 1 year of follow-up, 92.5% (37/40) were MRD-negative with MNavigator V2 assay. These three patients who detected MRD-positive after surgery, ctDNA cleared after adjuvant therapy, and persistently negative during follow-up, may benefit from treatment. Conclusion: Based on the patient-specific tumor mutation profile, the personalized MRD detection assay achieved better clinical performance than the fixed panel strategy. Citation Format: Shunying Li, Wei Gao, Ning Fu, Wuqiang Cao, Xiaoling Zeng, Xinhua Du, Qiang Liu. Tissue-informed personalized MRD detection assay may outperform tumor-informed fixed panel strategy in Triple Negative Breast Cancer (TNBC) [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO5-16-04.

Development of a standardised framework with universal core indicators for flood resilience assessment

Development of a standardised framework with universal core indicators for flood resilience assessment

The Effect of Mindfulness on the Promotion of Graduate Students' Scientific Research Creativity: The Chain Mediating Role of Flow Experience and Creative Self-Efficacy.

Creativity is a universal core higher-order cognitive ability in the 21st century, which reflects a country's core competitiveness and soft power. Mindfulness, as an important concept in positive psychology, has also received attention for its potential effect on research creativity. Using structural equation modeling and bootstrap methods, this study investigated the relationship between mindfulness and research creativity among 1210 Chinese graduate students. Additionally, we explored the mediating effect of flow experience and creative self-efficacy in this relationship. The research results showed that mindfulness had a direct positive effect on graduate students' scientific research creativity. The mediating effect of flow experience and creative self-efficacy, as well as the chain mediating effect, were established, with the mediating ratio being 13.1% and 30.0%, respectively, and the indirect effect of chain mediating accounting for 34.1%. Interestingly, the effect mechanism at the gender level was various, with the mediating effect of mindfulness on scientific research creativity being higher among male graduate students. The results of this study revealed the mechanism of mindfulness on graduate students' scientific research creativity, offering valuable insights for enhancing their creative capabilities in the realm of scientific research.

ИССЛЕДОВАНИЕ СЫПУЧЕСТИ ЗЕРНА И РАЗРАБОТКА УНИВЕРСАЛЬНОГО АЭРАТОРА КОЛОНКОВОГО ТИПА

The article studies physical property of grain flowability at free or natural grain flow when pouring through a small hole onto a flat horizontal plane, as well as when grain is in cramped conditions. The study classifies grains into 5 classes according to their flowability depending on their natural slope of repose. It also determines limiting values of slope of repose of basic grains at humidity of 14–16 % and with free flow on the designed device. The research detects penetration of grain into the partition space when grain was cramped into the designed device. It figures out that with increasing distance between the partitions, depth of grain penetration increases regardless of the partition grade of slope. Optimal values of partition grade of slope are within 20º, at which the penetration of grain between partitions is minimal. So, to solve various problems of grain aeration, it is possible to select the optimal values of partitions grade of slope and distances between them, at which grain does not flow into partitions internal cavity, connected to air supply channel. Based on the results of this experiment, the Department of Technological Systems of the Agro-Industrial Complex (Federal State Budgetary Educational Institution of Higher Education Arctic State Technical University) developed a technical solution for universal core type aerator, which was recognized as an invention (Patent N 2777902). Preliminary tests of the aerator showed its versatility and cost-effectiveness.

Multi-variate coding for possession: methodology and preliminary results.

In this work we are presenting a database structure to encode the phenomenon of differential possession across languages, considering noun possession classes and possessive constructions as independent but linked. We show how this structure can be used to study different dimensions of possession: semantics, noun valence, and possessive constructions. We present preliminary survey results from a global sample of 120 languages and show that there is a universal semantic core in both inalienable and non-possessible noun classes. Inalienables are centered on body parts and kinship. Non-possessibles are centered on animals, humans, and natural elements.

A horizontal view of primary metabolomes in vegetative desiccation tolerance.

Vegetative desiccation tolerance (VDT), the ability of such tissues to survive the near complete loss of cellular water, is a rare but polyphyletic phenotype. It is a complex multifactorial trait, typified by universal (core) factors but with many and varied adaptations due to plant architecture, biochemistry and biotic/abiotic dynamics of particular ecological niches. The ability to enter into a quiescent biophysically stable state is what ultimately determines desiccation tolerance. Thus, understanding the metabolomic complement of plants with VDT gives insight into the nature of survival as well as evolutionary aspects of VDT. In this study, we measured the soluble carbohydrate profiles and the polar, TMS-derivatisable metabolomes of 7 phylogenetically diverse species with VDT, in contrast with two desiccation sensitive (DS) species, under conditions of full hydration, severe water deficit stress, and desiccation. Our study confirmed the existence of core mechanisms of VDT systems associated with either constitutively abundant trehalose or the accumulation of raffinose family oligosaccharides and sucrose, with threshold ratios conditioned by other features of the metabolome. DS systems did not meet these ratios. Considerable chemical variations among VDT species suggest that co-occurring but distinct stresses (e.g., photooxidative stress) are dealt with using different chemical regimes. Furthermore, differences in the timing of metabolic shifts suggest there is not a single "desiccation programme" but that subprocesses are coordinated differently at different drying phases. There are likely to be constraints on the composition of a viable dry state and how different adaptive strategies interact with the biophysical constraints of VDT.

A global core outcome measurement set for snakebite clinical trials

Snakebite clinical trials have often used heterogeneous outcome measures and there is an urgent need for standardisation. A globally representative group of key stakeholders came together to reach consensus on a globally relevant set of core outcome measurements. Outcome domains and outcome measurement instruments were identified through searching the literature and a systematic review of snakebite clinical trials. Outcome domains were shortlisted by use of a questionnaire and consensus was reached among stakeholders and the patient group through facilitated discussions and voting. Five universal core outcome measures should be included in all future snakebite clinical trials: mortality, WHO disability assessment scale, patient-specific functional scale, acute allergic reaction by Brown criteria, and serum sickness by formal criteria. Additional syndrome-specific core outcome measures should be used depending on the biting species. This core outcome measurement set provides global standardisation, supports the priorities of patients and clinicians, enables meta-analysis, and is appropriate for use in low-income and middle-income settings.

Ribosomal Protein Cluster Organization in Asgard Archaea.

It has been proposed that the superphylum of Asgard Archaea may represent a historical link between the Archaea and Eukarya. Following the discovery of the Archaea, it was soon appreciated that archaeal ribosomes were more similar to those of Eukarya rather than Bacteria. Coupled with other eukaryotic-like features, it has been suggested that the Asgard Archaea may be directly linked to eukaryotes. However, the genomes of Bacteria and non-Asgard Archaea generally organize ribosome-related genes into clusters that likely function as operons. In contrast, eukaryotes typically do not employ an operon strategy. To gain further insight into conservation of the r-protein genes, the genome order of conserved ribosomal protein (r-protein) coding genes was identified in 17 Asgard genomes (thirteen complete genomes and four genomes with less than 20 contigs) and compared with those found previously in non-Asgard archaeal and bacterial genomes. A universal core of two clusters of 14 and 4 cooccurring r-proteins, respectively, was identified in both the Asgard and non-Asgard Archaea. The equivalent genes in the E. coli version of the cluster are found in the S10 and spc operons. The large cluster of 14 r-protein genes (uS19-uL22-uS3-uL29-uS17 from the S10 operon and uL14-uL24-uL5-uS14-uS8-uL6-uL18-uS5-uL30-uL15 from the spc operon) occurs as a complete set in the genomes of thirteen Asgard genomes (five Lokiarchaeotes, three Heimdallarchaeotes, one Odinarchaeote, and four Thorarchaeotes). Four less conserved clusters with partial bacterial equivalents were found in the Asgard. These were the L30e (str operon in Bacteria) cluster, the L18e (alpha operon in Bacteria) cluster, the S24e-S27ae-rpoE1 cluster, and the L31e, L12..L1 cluster. Finally, a new cluster referred to as L7ae was identified. In many cases, r-protein gene clusters/operons are less conserved in their organization in the Asgard group than in other Archaea. If this is generally true for nonribosomal gene clusters, the results may have implications for the history of genome organization. In particular, there may have been an early transition to or from the operon approach to genome organization. Other nonribosomal cellular features may support different relationships. For this reason, it may be important to consider ribosome features separately.

How to Avoid the Cultural Distortion Risk at Heritage Sites? A Configuration Analysis Perspective

Cultural distortion risk (CDR) threatens and challenges the cultural sustainability of heritage sites. This study adopted a sequential exploratory mixed-method design comprising grounded theory and fuzzy-set qualitative comparative analysis (fsQCA) to analyze the influencing factors, formation process, and avoidance paths of cultural distortion risk. The results indicate that CDR is caused by the “institution-market-production” framework, which involves eight influencing factors and 21 indicators. There are no necessary conditions for CDR formation, but weak cultural production plays a universal core role in forming high CDR. Moreover, our study highlights that the generation logic of CDR consists of four configurations and that the avoidance paths cover three configurations. These findings clarify the generation mechanism of CDR and expand the theoretical system of cultural sustainability research from a risk perspective. Heritage sites can use 21 indicators to reduce the cultural distortion risks by establishing institutional guarantee mechanisms and strengthening multiparty collaborative governance.

Life as a Guide to Its Own Origins

The origin of life entails a continuum from simple prebiotic chemistry to cells with genes and molecular machines. Using life as a guide to this continuum, we consider how selection could promote increased complexity before the emergence of genes. Structured, far-from-equilibrium environments such as hydrothermal systems drive the reaction between CO2 and H2 to form organics that self-organize into protocells. CO2 fixation within protocells generates a reaction network with a topology that prefigures the universal core of metabolism. Positive feedback loops amplify flux through this network, giving a metabolic heredity that promotes growth. Patterns in the genetic code show that genes and proteins arose through direct biophysical interactions between amino acids and nucleotides in this protometabolic network. Random genetic sequences template nonrandom peptides, producing selectable function in growing protocells. This context-dependent emergence of information gives rise seamlessly to an autotrophic last universal common ancestor.

СМИ как отражение современных требований общества к универсальным компетенциям выпускников университетов

The article examines the modern requirements of society to the universal competencies of university graduates through the prism of publications in the leading media. Employers expect professionals from universities with a set of necessary professional and supra-professional competencies. Modern Russian media reflect discussions in academic, scientific, and professional circles that affect the requirements of employers and society for professional knowledge and universal core competencies imposed on young employees.

Selective translational control of cellular and viral mRNAs by RPS3 mRNA binding.

RPS3, a universal core component of the 40S ribosomal subunit, interacts with mRNA at the entry channel. Whether RPS3 mRNA-binding contributes to specific mRNA translation and ribosome specialization in mammalian cells is unknown. Here we mutated RPS3 mRNA-contacting residues R116, R146 and K148 and report their impact on cellular and viral translation. R116D weakened cap-proximal initiation and promoted leaky scanning, while R146D had the opposite effect. Additionally, R146D and K148D displayed contrasting effects on start-codon fidelity. Translatome analysis uncovered common differentially translated genes of which the downregulated set bears long 5'UTR and weak AUG context, suggesting a stabilizing role during scanning and AUG selection. We identified an RPS3-dependent regulatory sequence (RPS3RS) in the sub-genomic 5'UTR of SARS-CoV-2 consisting of a CUG initiation codon and a downstream element that is also the viral transcription regulatory sequence (TRS). Furthermore, RPS3 mRNA-binding residues are essential for SARS-CoV-2 NSP1-mediated inhibition of host translation and for its ribosomal binding. Intriguingly, NSP1-induced mRNA degradation was also reduced in R116D cells, indicating that mRNA decay occurs in the ribosome context. Thus, RPS3 mRNA-binding residues have multiple translation regulatory functions and are exploited by SARS-CoV-2 in various ways to influence host and viral mRNA translation and stability.

Linguistic Relativism and the Expression of Basic Theoretical Rationality in Inuktitut

This paper begins by stressing the importance of distinguishing between linguistic relativity, whose reality is hardly debatable, and linguistic relativism, which ultimately holds that language communities are locked within their own worldview, hence their own notions of truth. It then rejects linguistic relativism by asserting the existence of a universal core of theoretical rationality comprised at the very least of the logical principles of identity, noncontradiction, and excluded middle. It goes on to show how this theoretical rationality manifests itself in the lexicon of a language that differs greatly from English: Inuktitut spoken by Inuit of Nunavik (Arctic Quebec). The definitions provided by Taamusi Qumaq for three words relating to critical thought are translated for the first time. Other quotes give examples of theoretical rationality in actual usage. Finally, the paper asks why logic as such is accorded little value in the culture Inuktitut expresses. The suggested answer follows Jack Goody, who holds that a long written tradition is required for the rules of formal logic to take hold in language practices. Writing is not just a transcription of the spoken word; it is an intellectual technology that impacts the way speakers use their language.

Tumor-informed patient-specific MRD monitoring analysis in colorectal cancer.

48 Background: Growing studies have suggested that personalized tumor-informed circulating tumor DNA (ctDNA) based minimal residual disease (MRD) detection can precisely predict recurrence in resectable patients (pts) with colorectal cancer (CRC). We developed an improved approach, a Tumor-Informed Patient-specific panel integrating with a tiny tumor-specific Core panel (TIPC) to monitor MRD by ultra-deep sequencing of plasma samples. The combination with a core panel confers TIPC overcoming the tumor temporal and spatial heterogeneity to some extent and providing more comprehensive information to guide individualized management. Here, we investigated the clinical utility of this assay and performed a head-to-head comparison with a Tumor-Informed Off-the-shelf panel approach (TIO). Methods: The retrospective study enrolled 34 I-IV CRC patients undergoing curative intent surgery from multiple centers and a median follow-up of 482 days. Tissue samples were collected at surgery. Peripheral blood samples collected postoperative and before relapse were analyzed. For all patients, at least one sample was available within 6 months before progression, defined as surveillance time point. TIPC is a whole exome sequencing (WES) based approach to identify somatic mutations from tissue samples, followed by customizing a patient-specific capture panel covering up to 50 top-ranked variants, and then combined with a CRC universal core panel containing 10 actionable or high prevalence cancer-related genes to conduct ultra-deep sequencing above 100,000X of follow-up blood samples. TIO assay was performed in serial plasma samples by using an off-the-shelf 338 gene panel at a sequencing depth of 30,000X. Results: As of August 31, 2022, 25 pts had confirmed recurrence. For the surveillance time point, 24/25 (96.0%) disease recurrence pts were detected MRD positive with TIPC assay, meanwhile, all disease-free pts were MRD negative (100%, 9/9). MRD detection preceded radiographic progression (median 5.6 months). TIO approach showed the same MRD detection specificity performance as TIPC, whereas the sensitivity was 88.0% (22/25). The ctDNA fraction of these two TIO undetectable pts was quantified using TIPC were 0.0168% and 0.0177%, while the positive mutations were beyond the 338 gene panel. The detectable postoperative ctDNA was associated with a poor prognosis for patients by either TIPC or TIO assay, with hazard ratios 20.8 (p＜0.0001) and 7.8 (p＜0.0001) respectively. To further verify TIPC may possess higher sensitivity, another cohort of 4 radiographic progressed CRC pts with TIO longitudinal undetectable MRD was analyzed, 3/4 (75.0%) was detectable by TIPC (ctDNA fraction range 0.0006%-0.0075%). Conclusions: WES-based patient-specific MRD detection assay demonstrated encouraging recurrence risk stratification value in CRC pts and may pave the way for the management of individual treatment.

Универсализм наскального искусства: обращение к первоосновам культуры

The cultural comprehension rock art monuments are of gains special relevance. Due to the need to evaluate this cultural text in the context of the changed socio-cultural peculiarities of life. In rock paintings depicted the oldest strata of human consciousness, which influence to the culture of the nations, historically divided by time and space, and that indicates on generally anthropological quality of archaic art. In terms of methodology, the rock art is considered based on the principle of unity of the world historical process and the existence of a common universal core of human nature. With the help of image is designated by invisible and not perceived supernatural world of spirits, totemic ancestors, gods and other creatures. The contemporary complicating situation in the world forces us to look for answers to the most significant problems of life, turning, among other things, to the fundamental to the fundamentals culture: universalism, archaic and traditional syncretism, nature-centrism.