Unfiltered Surface Water Research Articles

Salinity and total suspended solids control mercury speciation in a tidal river: Comparisons with a photochemical mercury model

Daytime volatilization of gaseous elemental mercury (Hg(0)aq) is a significant mechanism for mercury removal from aquatic systems and potentially limits the production and bioaccumulation of methylmercury. Changes in incoming solar radiation (in the ultraviolet range), dissolved organic matter, salinity, and total suspended particles were investigated concurrently with several mercury species (Hg(0)aq, dissolved total mercury (THg), easily reducible mercury (ERM), and mercury associated with total suspended solids (THgTSS)) during daylight hours near the mouth of a hypertidal river. There were no predictable temporal changes observed for Hg(0)aq in unfiltered surface water. Hg(0)aq ranged from 0 to 12 pg L−1, THg ranged from 0 to 492 pg L−1, ERM ranged from 13 to 381 pg L−1, and THgTSS ranged from <1.58 ng g−1 to 261.32 ng g−1. The range of Hg(0)aq predicted by the empirical model was similar to measured ERM concentrations, but it was shown that ERM did not significantly predict in-situ photoreducible Hg(II) (Hg(II)RED). Production of Hg(0)aq appears to largely be suppressed by suspended solids, which limits ultraviolet radiation transmission through surface water. Comparison of these results to an empirical model developed for this site to predict Hg(0)aq indicates that significantly more mercury is available for photoreduction near the mouth of the tidal river, and that Hg(II) will likely photoreduce quickly when TSS levels decrease with ocean mixing.

Stability issues of microcystins, anabaenopeptins, anatoxins, and cylindrospermopsin during short-term and long-term storage of surface water and drinking water samples

Reproducible analytical procedures and rigorous quality control are imperative for an accurate monitoring of cyanobacterial toxins in environmental water samples. In this study, the short-term and long-term storage stability of diverse cyanotoxins (anatoxins, cylindrospermopsin, anabaenopeptins, and 12 microcystins) was evaluated in water samples, under different scenarios. Transport controls were performed at three monitoring sites in spiked ultrapure water and lake water to investigate short-term stability issues. Medium-term storage stability was evaluated for up to 14–28 days in ultrapure water, chlorine-treated drinking water (amended with reductant), and surface water (filtered and unfiltered) stored at different temperatures (20 °C, 4 °C, and –20 °C). Substantial decreases of cylindrospermopsin and anabaenopeptins were observed in tap water (20 °C) and unfiltered surface water (20 °C or 4 °C). Regardless of matrix type, cyanotoxin recoveries generally remained within an 80–120% range when the water samples were kept frozen. After a prolonged storage duration of 365 days at –20 °C, most cyanotoxins experienced decreases in the range of 10–20%. The notable exception was for the tryptophan-containing MC-LW and MC-WR, with more substantial variations (30% to 50% decrease) and conversion to N-formylkynurenine analogs. Reanalysis of field-collected surface waters after long-term storage at –20 °C also indicated significantly decreasing trends of cyanotoxins (between 6% and 23% decrease). In view of the above, short sample hold times should be favored as recommended in EPA methods.

Partition distribution of selected organochlorine pesticides in water, sediment pore water and surface sediment from uMngeni River, KwaZulu-Natal, South Africa

Organochlorine pesticides (OCPs) were analysed in surface water, pore water and surface sediment samples collected from the uMngeni River, which is one of the largest rivers in the province of KwaZulu-Natal, South Africa. Liquid-liquid extraction was used to extract the analytes from water and pore water samples and soxhlet extraction was used to extract sediment samples with subsequent florisil clean-up and gas chromatography-mass spectrometry (GC-MS) analysis. Twelve selected OCPs were analysed and their total concentrations were found to range from 8.04–21.06 ng/mL, 36.06–188.43 ng/mL and 148.17–554.73 ng/g in unfiltered surface water, unfiltered pore water and surface sediment (dry weight (dw)), respectively. The results indicated that the concentrations of these selected pesticides were far higher in sediment (72%) than in pore water (25%) and water (3%). The most polluted sites were Northern Wastewater Treatment influent (NWTI) for water (Σ12 OCP = 19.41 ± 1.43 ng/mL) and Northern Wastewater Treatment effluent (NWTE) for pore water (Σ12 OCP = 166.23 ± 7.16 ng/mL) and sediment (Σ12 OCP = 495.21 ± 32.38 ng/g). The most abundant individual OCPs and their average concentrations in general in the river were p,p′-DDE in unfiltered water (1.62 ±0.22 ng/mL) and unfiltered sediment pore water (17.09 ±7.96 ng/mL), and endrin in surface sediment (55.57 ± 19.01 ng/g, dw).

Sub-daily rates of degradation of fluvial carbon from a peat headwater stream

In-stream processing of allochthonous dissolved organic carbon (DOC) and particulate organic carbon (POC) in peat-sourced headwaters has been shown to be a significant part of the terrestrial carbon cycle, through photo- and bio-degradation, with both DOC and POC converted to carbon dioxide (CO2). This study reports a series of 70-h, in situ experiments investigating rates of degradation in unfiltered surface water from a headwater stream in the River Tees, North Pennines, UK. Half the samples were exposed to the normal day/night cycle (ambient); half were continuously dark. The study found that the DOC concentration of samples in the ambient treatment declined by 64 % over the 70 h, compared with 6 % decline for the samples kept in the dark. For POC, the loss in the ambient treatment was 13 %. The average initial rate of loss of DOC in the ambient treatment during the first day of the experiment was 3.36 mg C/l/h, and the average rate of photo-induced loss over the whole 70 h was 1.25 mg C/l/h. Scaling up these losses, the estimate of total organic carbon loss from UK rivers to the atmosphere is 9.4 Tg CO2/year which would be 0.94 % of the global estimate of CO2 emissions from streams and rivers from the 2013 IPCC report. Initial rate kinetics in the light were as high as 3rd order, but the study showed that no single rate law could describe the whole diurnal degradation cycle and that separate rate laws were required for night and day processes. The comparison of dark and ambient treatment processes showed no evidence of photo-stimulated bacterial degradation.

Occurrence and significance of polychlorinated biphenyls in water, sediment pore water and surface sediments of Umgeni River, KwaZulu-Natal, South Africa.

The Umgeni River is one of the main sources of water in KwaZulu-Natal, South Africa; however; there is currently a lack of information on the presence and distribution of polychlorinated biphenyls (PCBs) in its sediment, sediment pore water and surface water. This study aims to determine the occurrence and significance of selected PCBs in the surface water, sediment pore water and surface sediment samples from the Umgeni River. Liquid-liquid and soxhlet extractions were used for water or pore water, and sediments, respectively. Extracts were cleaned up using a florisil column and analysed by gas chromatography-mass spectrometry. The total concentrations of eight polychlorinated biphenyls were 6.91-21.69 ng/mL, 40.67-252.30 ng/mL and 102.60-427.80 ng/g (dry weight), in unfiltered surface water, unfiltered sediment pore water and surface sediments, respectively. The percentage contributions of various matrices were 4, 36 and 60 % for unfiltered surface water, unfiltered pore water and sediment, respectively. The highest concentrations of PCBs were found in water, pore water and sediment collected from sampling sites close to the Northern Wastewater Treatment Works. The highest chlorinated biphenyl, PCB 180, was the most abundant at almost all sampling sites. To our knowledge, this is the first report on occurrence of polychlorinated biphenyls in the Umgeni River water, pore water and sediment system and our results provide valuable information regarding the partitioning of the PCBs between the water and sediment systems as well as the organic chemical quality of the water.

Long-term monitoring of arsenic, copper, selenium, and other elements in Great Salt Lake (Utah, USA) surface water, brine shrimp, and brine flies.

This paper presents long-term monitoring data for 19 elements with a focus on arsenic (As), copper (Cu), and selenium (Se), in surface water (2002-2011), brine shrimp (2001-2011), and brine flies (1995-1996) collected from Great Salt Lake (GSL, Utah, USA). In open surface waters, mean (±standard deviation [SD]; range; n) As concentrations were 112 (±22.1; 54.0-169; 47) and 112 μg/L (±35.6; 5.1-175; 68) in filtered and unfiltered surface water samples, respectively, and 16.3 μg/g (±5.6; 5.1-35.2; 62) dry weight (dw) in brine shrimp. Mean (±SD; range; n) Cu concentrations were 4.2 (±2.1; 1.3-12.5; 47) and 6.9 μg/L (±6.6; 1.9-38.1; 68) in filtered and unfiltered surface water samples, respectively, and 20.6 μg/g (±18.4; 5.4-126; 62) dw in brine shrimp. Finally, mean (±SD; range; n) dissolved and total recoverable Se concentrations were 0.6 (±0.1; 0.4-1.2; 61) and 0.9 μg/L (±0.7; 0.5-3.6; 89), respectively, and 3.6 μg/g (±2.2; 1.1-14.9; 98) dw in brine shrimp. Thus, Se in open lake surface waters was most often in the range of 0.5-1 μg/L, and concentrations in both surface water and brine shrimp were comparable to concentrations measured in other monitoring programs for the GSL. Temporally, the statistical significance of differences in mean dissolved or total recoverable As, Cu, and Se concentrations between years was highly variable depending which test statistic was used, and there was no clear evidence of increasing or decreasing trends. In brine shrimp, significant differences in annual mean concentrations of As, Cu, and Se were observed using both parametric and nonparametric statistical approaches, but, as for water, there did not appear to be a consistent increase or decrease in concentrations of these elements over time.

Mercury cycling in agricultural and managed wetlands, Yolo Bypass, California: Spatial and seasonal variations in water quality

The seasonal and spatial variability of water quality, including mercury species, was evaluated in agricultural and managed, non-agricultural wetlands in the Yolo Bypass Wildlife Area, an area managed for multiple beneficial uses including bird habitat and rice farming. The study was conducted during an 11-month period (June 2007 to April 2008) that included a summer growing season and flooded conditions during winter. Methylmercury (MeHg) concentrations in surface water varied over a wide range (0.1 to 37ngL−1 unfiltered; 0.04 to 7.3ngL−1 filtered). Maximum MeHg values are among the highest ever recorded in wetlands. Highest MeHg concentrations in unfiltered surface water were observed in drainage from wild rice fields during harvest (September 2007), and in white rice fields with decomposing rice straw during regional flooding (February 2008). The ratio of MeHg to total mercury (MeHg/THg) increased about 20-fold in both unfiltered and filtered water during the growing season (June to August 2007) in the white and wild rice fields, and about 5-fold in fallow fields (July to August 2007), while there was little to no change in MeHg/THg in the permanent wetland. Sulfate-bearing fertilizer had no effect on Hg(II) methylation, as sulfate-reducing bacteria were not sulfate-limited in these agricultural wetlands. Concentrations of MeHg in filtered and unfiltered water correlated with filtered Fe, filtered Mn, DOC, and two indicators of sulfate reduction: the SO42−/Cl− ratio, and δ34S in aqueous sulfate. These relationships suggest that microbial reduction of SO42−, Fe(III), and possibly Mn(IV) may contribute to net Hg(II)-methylation in this setting.

Sunlight Inactivation of Human Viruses and Bacteriophages in Coastal Waters Containing Natural Photosensitizers

Sunlight inactivation of poliovirus type 3 (PV3), adenovirus type 2 (HAdV2), and two bacteriophage (MS2 and PRD1) was investigated in an array of coastal waters to better understand solar inactivation mechanisms and the effect of natural water constituents on observed inactivation rates (k(obs)). Reactor scale inactivation experiments were conducted using a solar simulator, and k(obs) for each virus was measured in a sensitizer-free control and five unfiltered surface water samples collected from different sources. k(obs) values varied between viruses in the same water matrix, and for each virus in different matrices, with PV3 having the fastest and MS2 the slowest k(obs) in all waters. When exposed to full-spectrum sunlight, the presence of photosensitizers increased k(obs) of HAdV2, PRD1 and MS2, but not PV3, which provides evidence that the exogenous sunlight inactivation mechanism, involving damage by exogenously produced reactive intermediates, played a greater role for these viruses. While PV3 inactivation was observed to be dominated by endogenous mechanisms, this may be due to a masking of exogenous k(obs) by significantly faster endogenous k(obs). Results illustrate that differences in water composition can shift absolute and relative inactivation rates of viruses, which has important implications for natural wastewater treatment systems, solar disinfection (SODIS), and the use of indicator organisms for monitoring water quality.

The importance of waterborne disease outbreak surveillance in the United States

Analyses of the causes of disease outbreaks associated with contaminated drinking water in the United States have helped inform prevention efforts at the national, state, and local levels. This article describes the changing nature of disease outbreaks in public water systems during 1971-2008 and discusses the importance of a collaborative waterborne outbreak surveillance system established in 1971. Increasing reports of outbreaks throughout the early 1980s emphasized that microbial contaminants remained a health-risk challenge for suppliers of drinking water. Outbreak investigations identified the responsible etiologic agents and deficiencies in the treatment and distribution of drinking water, especially the high risk associated with unfiltered surface water systems. Surveillance information was important in establishing an effective research program that guided government regulations and industry actions to improve drinking water quality. Recent surveillance statistics suggest that prevention efforts based on these research findings have been effective in reducing outbreak risks especially for surface water systems.

Case Study of Particle-Related UV Shielding of Microorganisms When Disinfecting Unfiltered Surface Water

Abstract The goal of this case study was to provide pilot-scale information about the ability of ultraviolet (UV) light to disinfect unfiltered surface water. A pilot-scale (0.25 L/s) UV reactor with low-pressure UV lamps was installed on raw water entering an aqueduct from the Pardee Reservoir at the East Bay Municipal District, California. A pilot monitoring system also collected hourly particle count (2 to 100 m), turbidity, and ultraviolet transmittance (UVT) measurements for 14 months. Grab microbial samples were collected and analyzed for indigenous total coliforms and total aerobic spores (TAS) both before and after UV disinfection, to correlate survival of the organisms across the UV reactor to water quality characteristics. Concentrations of indigenous coliforms and TAS ranged up to 163 and 1,383 per 100 mL respectively, before UV exposure. The data showed that the ability of UV to disinfect coliforms was essentially unaffected by the presence of particles (up to 703&amp;gt;10 m per mL and 1.3 nephelometric turbidity unit [NTU]) in the unfiltered surface water. In 13 of 14 samples, no coliforms were detected in the UV treated water. Log-linear inactivation of TAS up to 2.5-log suggests that at least 99.6% of the TAS were not protected from UV disinfection by particles.

Determination of the Sum of Short Chain Polychlorinated n‐Alkanes with a Chlorine Content of Between 49 and 67% in Water by GC‐ECNI‐MS and Quantification by Multiple Linear Regression

AbstractThis new approach for the quantification of the sum of short chain polychlorinated alkanes in mixtures (commonly abbreviated as SCCPs, where P stands for paraffin) with average chlorine contents between 49 and 67% and different C‐number distributions is the background for ISO CD 12010, allowing standardized analysis according to the European water framework directive in unfiltered surface water, ground water, drinking water and waste water. A quantification method for the sum of short chain polychlorinated n‐alkanes by GC‐ECNI‐MS (gas chromatography coupled with electron capture chemical ionization mass spectrometry), independent of the chlorine content and robust against changes in C‐number distribution, was developed. This means that the very different response factors in GC‐ECNI‐MS, depending on the chlorine content, are no longer a problem for the analysis of SCCPs. Furthermore, problems with the selection of analytical standards due to batch dependent technical mixtures and different environmentally occurring mixtures can be overcome by using this robust method. The calibration was performed using synthetic mixtures mimicking a variety of different technically produced SCCP mixtures with average chlorine contents between 49 and 67% and different C‐number distributions. By this means, the calibration is also traceable. The method was based on the integration of selected m/z value (mass/charge relation value) signals over the full retention range of the compounds. Quantification was performed using multiple linear regression calibration with two selected m/z signals, and verified with two other mass combinations. The selection of the m/z values used for quantification was carried out by means of an orthogonal calibration design. A stepwise procedure was utilized to produce the best quantification results, with the minimum number of quantification m/z signals, together with the minimization of potential interferences to be encountered in water analysis. The weighted sum of the peak areas of two m/z values, m/z 327 and 423, led to a sum determination which was applicable to the different SCCP mixtures most likely to be found in the aquatic environment. The problem with the very different response factors of the selected m/z values in GC‐ECNI‐MS, caused by the differing chlorine content of the SCCP mixtures, was overcome as well by forming the weighted sum of the two selected peak areas. The method was especially suitable for water analysis because of the low detection limit achieved by GC‐ECNI‐MS for chlorinated compounds.

Sources and seasonal patterns of dissolved organic matter (DOM) in the hyporheic zone

The hyporheic zone is a region underneath streambeds that integrates surface and groundwater. Although its location is central to biogeochemical linkages between the riparian zone, dissolved nutrients, and benthic biota, the seasonal quality and likely sources of dissolved organic matter (DOM) in the hyporheic zone are not well understood. To investigate DOM characteristics in the hyporheic zone, water from the surface and subsurface (at depths 20, 60, and 100 cm below the streambed) was sampled every 4 weeks from 2007 to 2008 in a third-order stream in southern Ontario. Using UV spectroscopy, measures of spectral slopes, aromaticity, and A 254/A 365 ratios (molecular weight) were obtained. Temporal changes in these measures were consistent with watershed processes such as shedding of leaf litter in the fall, and photochemical and biofilm influence in the spring and summer. The fluorescence index (a measure of relative DOM source) suggested that at the surface and in the downwelling zone, DOM microbial sources increased with depth in the sediment, regardless of the season. Excitation–emission matrices (EEMs) showed seasonally distinct, protein-like DOM components of bacterial origin that were stronger in the fall. Leachates from specific allochthonous DOM sources—leaf litter from Betula papyrifera (white birch) and Thuja occidentalis (white cedar)—and an autochthonous source, biofilm, were isolated and incubated with unfiltered surface water. EEMs from these leachates indicated that these sources could indeed help explain observed patterns of DOM in surface and subsurface waters. These results suggest that although DOM sources were relatively constant, biogeochemical processing within the hyporheic zone resulted a DOM pool that was temporally dynamic and altered the nature of organic matter transported downstream into lakes and rivers.

A comparison of mercury cycling in Lakes Michigan and Superior

Mercury cycling in Lake Superior and Lake Michigan was evaluated based on measurements of mercury levels, modeling of evasional fluxes, and development of first-order mass balance models. Total mercury, methylmercury, and dissolved gaseous mercury were measured on sampling cruises in Lake Michigan (2005) and Lake Superior (2006). Average total mercury concentrations in unfiltered surface water were higher in Lake Michigan (420 ± 40 pg/L) compared to Lake Superior (210 ± 20 pg/L). Methylmercury levels were below the detection limit in Lake Michigan. Larger sample volumes were collected to lower detection limits in Lake Superior in 2006 and methylmercury levels averaged 7 ± 6 pg/L. Dissolved gaseous mercury concentrations were also higher in Lake Michigan (27 ± 7 pg/L) compared to Lake Superior (14 ± 8 pg/L). Evasional fluxes were estimated using a two-film model for air–water exchange. The annual evasional flux in Lake Michigan was determined to be ~ 380 kg/yr from Lake Michigan and ~ 160 kg/yr from Lake Superior. Total mercury burdens in each lake were estimated to be ~ 2500 kg in Superior and ~ 2100 kg in Lake Michigan demonstrating that evasional fluxes play an important role in the mass balance of each lake, particularly Lake Michigan. A simple first-order mass balance model demonstrates the importance of air–water exchange and sedimentation as primary removal processes for Hg in each lake. Uncertainties in the mass balance model are highlighted due to lack of key data, particularly in Lake Superior.

Submarine Groundwater Discharge of Total Mercury and Monomethylmercury to Central California Coastal Waters

Fluxes of total mercury (Hg(T)) and monomethylmercury (MMHg) associated with submarine groundwater discharge (SGD) at two sites onthe central California coast were estimated by combining measurements of Hg(T) and MMHg in groundwater with the use of short-lived, naturally occurring radium isotopes as tracers of groundwater inputs. Concentrations of Hg(T) were relatively low, ranging from 1.2 to 28.3 pM in filtered groundwater, 0.8 to 11.6 pM in filtered surface waters, and 2.5 to 12.9 pM in unfiltered surface waters. Concentrations of MMHg ranged from < 0.04 to 3.1 pM in filtered groundwater, < 0.04 to 0.53 pM in filtered surface waters, and 0.07 to 1.2 pM in unfiltered surface waters. Multiple linear regression analysis identified significant (p < 0.05) positive correlations between dissolved groundwater concentrations of Hg(T) and those of NH4+ and SiO2, and between dissolved groundwater concentrations of MMHg and those of Hg(T) and NH4+. However, such relationships did not account for the majority of the variability in concentration data for either mercury species in groundwater. Fluxes of Hg(T) via SGD were estimated to be 250 +/- 160 nmol day m(-1) of shoreline at Stinson Beach and 3.0 +/- 2.0 nmol m(-2) day(-1) at Elkhorn Slough. These Hg(T) fluxes are substantially greater than net atmospheric inputs of Hg(T) reported for waters in nearby San Francisco Bay. Calculated fluxes of MMHg to coastal waters via SGD were 10 +/- 12 nmol day(-1) m(-1) of shoreline at Stinson Beach and 0.24 +/- 0.21 nmol m(-2) day at Elkhorn Slough. These MMHg fluxes are similar to benthic fluxes of MMHg out of surface sediments commonly reported for estuarine and coastal environments. Consequently, this work demonstrates that SGD is an important source of both Hg(T) and MMHg to coastal waters along the central California coast.

Disinfection of Waterborne Coliform Bacteria by Neem Oil

Abstract This study assessed the ability of Neem oil to disinfect an Escherichia coli isolate in pure laboratory-grade water and total and fecal coliform bacteria in two unfiltered surface waters. Neem oil doses as low as 2.13 g·L−1 with 5 min of mixing time achieved high levels of inactivation of the E. coli in pure laboratory-grade water (>99% inactivation). However, the disinfection of total and fecal coliforms in the unfiltered surface waters was variable and limited to <70% inactivation typically. Increasing the Neem oil dose and mixing time generally resulted in an increase in the mean inactivation of total coliform bacteria but not always of fecal coliform bacteria. The inactivation of total coliforms was consistently greater than the inactivation of fecal coliforms, suggesting a potential range of sensitivities to Neem oil among coliform bacteria. Reduced effectiveness of the Neem oil in the unfiltered surface waters compared to the pure laboratory-grade water suggests a possible interference by n...

Inactivation of indigenous coliform bacteria in unfiltered surface water by ultraviolet light

This study examined the potential for naturally occurring particles to protect indigenous coliform from ultraviolet (UV) disinfection in four surface waters. Tailing in the UV dose–response curve of the bacteria was observed in 3 of the 4 water samples after 1.3–2.6-log of log-linear inactivation, implying particle-related protection. The impact of particles was confirmed by comparing coliform UV inactivation data for parallel filtered (11μm pore-size nylon filters) and unfiltered surface water. In samples from the Grand River (UVT: 65%/cm; 5.4 nephelometric turbidity units (NTU)) and the Rideau Canal (UVT: 60%/cm; 0.84NTU), a limit of ∼2.5log inactivation was achieved in the unfiltered samples for a UV dose of 20mJ/cm2 while both the filtered samples exhibited >3.4-log inactivation of indigenous coliform bacteria. The results suggest that particles as small as 11μm, naturally found in surface water with low turbidity (<3NTU), are able to harbor indigenous coliform bacteria and offer protection from low-pressure UV light.

Mercury distribution and transport in a contaminated river system in Kazakhstan and associated impacts on aquatic biota

Abstract The River Nura in Central Kazakhstan has been heavily polluted by Hg originating from an acetaldehyde plant. A number of studies were undertaken to investigate the transport, fate and bioavailability of Hg in this river system. The sediments within a 20 km section of the river downstream of the effluent outfall canal are highly polluted and are acting as a strong source of surface water contamination. Mercury transport in the river is dominated by the remobilization of contaminated bed sediments and river bank erosion during the annual spring flood. Peak Hg concentrations in unfiltered surface water samples during a larger than usual flood event in 2004 were in the order of 1600–4300 ng L−1. The majority of the particulate-bound Hg appears to be sedimented in the shallow Intumak reservoir ∼75 km downstream of the source of the pollution, leading to a drop in aqueous Hg concentrations by an order of magnitude. Nevertheless, background concentrations of Hg in surface water are not reached until at least 200 km downstream, and during the flood period Hg is also detected in the terminal wetlands of the river. Mercury concentrations in sediment cores taken from the river bed in the most contaminated section of the Nura ranged from 9.95 to 306 mg kg−1. Methylmercury (MeHg) levels in shallow sediment cores were highest in surface sediments and ranged between 4.9 and 39 μg kg−1, but were generally less than 0.1% of total Hg (THg). A significant inverse relationship was found between THg concentrations and the percentage of MeHg formed in the sediments, irrespective of the sampling depth. The observed relationship was confirmed by comparison with results from a different river system, indicating that it may be true also for other highly contaminated aquatic systems. It is hypothesized that at high THg levels in severely contaminated sediments, the accumulation of MeHg may be limited by increasingly efficient demethylation processes, and that this underlying trend in sediments is the reason why MeHg levels in surface water are often found to be higher at less contaminated sites compared to upstream sites. Mercury concentrations in biota in the most contaminated section of the river were 15–20 times higher than background levels. Fish were found to be impacted for more than 125 km downstream from the source, indicating significant transport of dissolved MeHg to downstream areas and/or in-situ MeHg production in less contaminated downstream reaches. There were also indications that impoundments may increase the bioavailability of Hg.

Estimates of endemic waterborne risks from community-intervention studies

The nature and magnitude of endemic waterborne disease are not well characterized in the United States. Epidemiologic studies of various designs can provide an estimate of the waterborne attributable risk along with other types of information. Community drinking water systems frequently improve their operations and may change drinking water treatment and their major source of water. In the United States, many of these treatment changes are the result of regulations promulgated under the Safe Drinking Water Act. A community-intervention study design takes advantage of these "natural" experiments to assess changes in health risks. In this paper, we review the community-intervention studies that have assessed changes in waterborne gastroenteritis risks among immunocompetent populations in industrialized countries. Published results are available from two studies in Australia, one study in the United Kingdom, and one study in the United States. Preliminary results from two other US studies are also available. Although the current information is limited, the risks reported in these community-intervention studies can help inform the national estimate of endemic waterborne gastroenteritis. Information is provided about endemic waterborne risks for unfiltered surface water sources and a groundwater under the influence of surface water. Community-intervention studies with recommended study modifications should be conducted to better estimate the benefits associated with improved drinking water treatment.

Risk of giardiasis associated with water supply in an endemic context

Consumption of untreated, inadequately treated or simply chlorinated water has been the source of a number of giardiasis epidemics. The aim of this study was to verify if water supply is associated with giardiasis in an endemic context. We conducted a case-control study that included 139 cases and 417 control subjects. To assess risk associated to water consumption, we calculated odds ratios (OR) using “source of water” and “presence and type of filtration” as criteria. A logistic regression model was used to control other risk factors. In children aged 1 – 13, the only significant risk factor was the consumption of at-risk tap water (i.e., filtered or unfiltered surface water or unfiltered shallow well water; OR = 6.13). In people aged 14 – 64, the consumption of this water was not a risk factor for the disease. The divergence in results between age groups could be explained by acquired immunity.

Variability of chromophoric organic matter in surface waters of the Atlantic Ocean

Abstract We report over 600 absorption spectra (250–800 nm) of unfiltered surface waters,