Underutilized Species Research Articles

Antioxidant Activity and Total Phenolic Content of Underutilized Edible Tree Species of the Philippines

Recently, neglected and underutilized species (NUS) have deservedly come to the forefront of scientific interest because they can contribute to the human nutrition, due to the content of bioactive substances such as antioxidants. Despite the great diversity and rich tradition in the use of Philippine NUS, the nutritional properties of many edible plants remain unexplored. The main objective of this study was to evaluate various parts of eleven NUS fruits and vegetables traditionally consumed in the Philippines, namely Allaeanthus luzonicus, Canarium ovatum, Dillenia philippinensis, Ficus pseudopalma, Flacourtia indica, Flacourtia inermis, Garcinia intermedia, Heliotropium arboreum, Posoqueria latifolia, Stelechocarpus burahol, and Sterculia quadrifida for their total phenolic content (TPC) and in vitro antioxidant activity (DPPH and ORAC assays). Inflorescence of A. luzonicus (DPPH IC50 = 91.0 μg/mL, ORAC IC50 = 37.9 μg/mL) and fruit of S. burahol (DPPH IC50 = 253.7 μg/mL, ORAC IC50 = 32.2 μg/mL) showed the strongest antioxidant activity in both assays. These two species also had the highest TPC (202.1 and 133.0 µg GAE/mg extract, respectively). For all samples tested, a strong correlation was found between TPC and antioxidant activity. Based on our results, A. luzonicus and S. burahol have promising potential as novel antioxidant rich food.

Evaluation of Lipid Damage, Microbial Spoilage and Sensory Acceptance of Chilled Pouting (Trisopterus luscus), an Underutilized Lean Fish Species

The present study focused on the use of pouting (Trisopterus luscus), an underutilized gadoid fish species, as a fresh product of potential commercial interest. Accordingly, non-degutted pouting specimens (145–195 g and 15–22 cm) were stored under chilling conditions (0 °C) for microbial, chemical and sensory analyses to evaluate their commercial quality and shelf life. A progressive quality loss (p < 0.05) was detected for this lean species (5.58 g lipids·kg−1 muscle) as the storage time increased, as determined through microbial (aerobes, psychrotrophs and Enterobacteriaceae counts), lipid hydrolysis (free fatty acid value), lipid oxidation (conjugated diene and triene, thiobarbituric acid reactive substance, and fluorescence values) and sensory acceptance assessment. A detailed comparison to related lean fish species revealed that the pouting exhibited a fast quality breakdown under refrigeration conditions. Thus, after 9 d of chilled storage, the psychrotroph counts exceeded the acceptable limits (8.54 log CFU·g−1), and the fish specimens were found to be rejectable, with the sensory panel, external odor and eye appearance being the limiting factors. In contrast, the pouting specimens exhibited high quality after 3 d of storage, with the quality being still acceptable after 6 d. According to the current search for novel, underutilized species, pouting is proposed as a promising source.

Storable, neglected, and underutilized species of Southern Africa for greater agricultural resilience.

The Southern African region suffers from drought and food system uncertainty with increased risks due to climate change, natural disasters, and global catastrophes. Increasing crop diversity with more appropriate and resilient crops is an effective way of increasing food system resilience. We focus on crop species that are native or naturalized to an area because of their increased resilience than those that are not naturally occurring. Additionally, crops that are easily stored are more useful in times of drought and disaster. In this systematic review, we use scientific interest in neglected and underutilized species (NUS) from Southern Africa to help define next steps toward their cultivation and development as a marketable crop. We found that although scientific interest is minimal for storable Southern African NUS, these crops are worth scaling up due to their economic and nutritional value. We outline next actionable steps and specific NUS for production in a more agrobiodiverse and resilient agriculture system.

NUS so fast: the social and ecological implications of a rapidly developing indigenous food economy in the Cape Town area

The modern global food system is highly unsustainable, shaped by industrialisation and corporate consolidation, with negative repercussions on the environment and biodiversity as well as human health. This paper looks at the burgeoning economy in neglected and underutilised species (NUS) in the Western Cape, South Africa, as a potential innovation that could make the local food system more socially and ecologically resilient. Although, at present, NUS are only marginally included in the local food system and policy discussions, NUS increasingly appear in the high-end food industry, driven by international gastronomic trends. These species hold potential as climate resilient, nutritionally dense, and socially and culturally significant foods in the region, but they also carry ecological and social risks. We critically examine the fledgling NUS economy in the Cape Town area to unpack the motivations and challenges associated with the potentially transformative inclusion of these foods into the local food system. We demonstrate that the main risks associated with NUS are negative ecological repercussions, privatisation of the NUS economy, and the reproduction and further entrenchment of unequal power and race dynamics in the region. To mitigate these risks and actualise the related benefits associated with NUS, engagement with the ecological, social, and political context of NUS needs to be significantly deepened. This is particularly true for those working in the high-end food industry, who appear to be driving the NUS economy, and will require education around sustainability and Traditional Ecological Knowledge (TEK), as well as a foregrounding awareness of power dynamics.

From 'Farm to Fork': Exploring the Potential of Nutrient-Rich and Stress-Resilient Emergent Crops for Sustainable and Healthy Food in the Mediterranean Region in the Face of Climate Change Challenges.

In the dynamic landscape of agriculture and food science, incorporating emergent crops appears as a pioneering solution for diversifying agriculture, unlocking possibilities for sustainable cultivation and nutritional bolstering food security, and creating economic prospects amid evolving environmental and market conditions with positive impacts on human health. This review explores the potential of utilizing emergent crops in Mediterranean environments under current climate scenarios, emphasizing the manifold benefits of agricultural and food system diversification and assessing the impact of environmental factors on their quality and consumer health. Through a deep exploration of the resilience, nutritional value, and health impacts of neglected and underutilized species (NUS) such as quinoa, amaranth, chia, moringa, buckwheat, millet, teff, hemp, or desert truffles, their capacity to thrive in the changing Mediterranean climate is highlighted, offering novel opportunities for agriculture and functional food development. By analysing how promoting agricultural diversification can enhance food system adaptability to evolving environmental conditions, fostering sustainability and resilience, we discuss recent findings that underscore the main benefits and limitations of these crops from agricultural, food science, and health perspectives, all crucial for responsible and sustainable adoption. Thus, by using a sustainable and holistic approach, this revision analyses how the integration of NUS crops into Mediterranean agrifood systems can enhance agriculture resilience and food quality addressing environmental, nutritional, biomedical, economic, and cultural dimensions, thereby mitigating the risks associated with monoculture practices and bolstering local economies and livelihoods under new climate scenarios.

The effectiveness of semi-wild Sumatran mango (Mangifera spp.) leaves as a phytotherapy agent for breast cancer

Background Breast cancer ranks first in the world, standing at a mortality rate of 24.5% per year and is the leading cause of cancer death in Indonesia. The current management of breast cancer therapy is considered less effective because of its careful use due to side effects that are detrimental to the patient. The semi-wild species from Sumatra are neglected and underutilized species but have the potential as a therapeutic agent. Previous research has revealed that this species of mango is high in antioxidant compounds. Objective This study was carried out to discover the anticancer activities of the semi-wild mango species via inhibitory activities and morphological changes in Michigan Cancer Foundation-7 (MCF-7) cells. Materials and methods The IC50 value of Mangifera sumatrana, Mangifera foetida, and Mangifera laurina leaves in n-hexane, ethyl-acetate, and methanol extracts was determined using the reagent 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Data were analyzed by two-way analysis of variance using IBM SPSS Statistics 21. Results and conclusion M. laurina n-hexane extracts exhibited anticancer activity (IC50 13.25 ppm). Nonpolar solutions were chosen as the most effective extraction solvent in anticancer tests because lipids in nonpolar solvents can hit the lipid bilayer. A hexane fraction was created by separating the majority of nonpolar fatty acid esters, and this fraction had a considerable impact on cytotoxic and apoptotic effects on MCF-7 cells. Therefore, all treatments can transform MCF-7 cells’ morphology into blackened dead cells that are degraded into small parts, such as apoptotic bodies in cells undergoing apoptotic processes. MTT assays against MCF-7 on three species of semi-wild Sumatran mango in different extraction solvents showed that n-hexane extracts of M. laurina had stronger anticancer activity than other samples. This study provides new information to support the development of standardized herbal medicines and phytopharmaca in the future.

Unveiling awareness and knowledge of orphan plants: catalysts for sustainable transformation in Al-Zarqa Basin, Jordan

The awareness of orphan plants and underutilized species that have agricultural, nutritional, and medicinal importance is critical to the sustainable civilization of people. The lack of knowledge of the orphan plant’s importance stems from insufficient outreach and educational efforts and this may increase the underappreciation of these plants’ ecological relevance which will affect the sustainable environment in the determined region. In this study, Al Zarqa basin region located in the northern part of Jordan was selected as a study case. This is due to it having rich plant biodiversity with many endangered plant species. This study aimed to assess the awareness levels of the civil people in the Al Zarqa basin regarding orphan plants and identify key factors influencing the community’s understanding of these underutilized species. In this study, local youth volunteers distributed an e-questionnaire, and 1000 participants from seven villages were engaged. Different parameters that measure people’s awareness regarding orphan plants were evaluated. Data were collected and statistically analyzed to show the range of the people’s awareness. Data showed the predominant of the samples used were male representation (66%), aged 18–45, engaged in agricultural and handicraft activities, with diverse educational backgrounds, and monthly incomes of 300–500 JD. Limited awareness (23.3%) of ‘orphan plants’ was linked to lower academic levels in the studied areas. Perceived benefits varied, with 36.1% acknowledging moderate advantages and 25.6% perceiving high benefits. Despite recognizing utility, participants lacked specificity in benefits or classification. The study concludes that the region exhibits a dearth of knowledge about orphan plants, necessitating educational initiatives to raise awareness and advocate for the agricultural and medicinal potential of underutilized plant species, contributing to the socioeconomic advancement of residents in the agriculturally endowed Zarqa Basin. This investigation is essential for highlighting the current state of awareness and informing potential outreach and education strategies. Addressing the root cause of underappreciation through targeted initiatives can play a pivotal role in fostering a better understanding of the ecological significance of rare and endangered plants, ultimately contributing to their conservation and sustainable use.

Exploring potentials to enhance fish sauce industry in Sri Lanka: A review

Fish sauce, an amber-colored liquid derived from the fermentation of fish with sea salt, is a vital condiment in Southeast Asian and East Asian cuisines. Despite its culinary importance, fish sauce remains underutilized in Sri Lanka, where it is predominantly imported, resulting in high costs and substantial foreign exchange outflows. The technology for fish sauce production is relatively simple, presenting an opportunity for local production using abundant fish resources. Various types of fish, including marine, freshwater, and shellfish, are used globally in fish sauce production, and it could harness the nutritional benefits of fish, transforming underutilized species into a valuable condiment. The production process of fish sauce involves salting, fermenting, maturing, filtering, and blending, resulting in a product rich in hydrolyzed proteins and essential minerals. Quality standards are regulated to ensure safety and purity, with a focus on maintaining the distinctive fishy odor and taste characteristic of pure fish sauce. Establishing a local fish sauce industry in Sri Lanka promises economic growth and culinary enrichment. By leveraging local fish resources and employing cost-effective production methods, Sri Lanka can reduce imports of fish sauce, and can utilize fish in a sustainable manner.

Secondary metabolite fingerprinting, anti-pathogenic activity, elite chemotype selection and conservation of Curcuma caesia- an ethnomedicinally underutilized species.

Curcuma caesia Roxb. is an ethnomedicinally important, essential oil (EO) yielding aromatic plant. A total of twelve accessions of this plant rhizome were collected from six different agro-climatic zones of West Bengal, India and evaluated for their antimicrobial activities against eight disease-causing, multi-drug-resistant pathogenic strains of urinary-tract infection and respiratory-tract infection. The EO and extracts demonstrated antibacterial activity, with the highest inhibition zone of 18.00 ± 0.08 and 17.50 ± 0.14mm against Klebsiella pneumoniae by accession 06, even where all the broad-spectrum antibiotics failed to respond. In this study, we employed high-performance thin-layer chromatography (HPTLC) to quantify curcumin, the primary secondary metabolite of C. caesia, and the highest 0.228mg/gm of curcumin resulted from accession 06. Hence, on the basis of all aspects, accession 06 was identified as the elite chemotype among all twelve accessions. The chemical profiling of EO from accession 06 was done using gas chromatography-mass spectroscopy (GC-MS). Conceivably, about 13 medicinally significant compounds were detected. As this plant species is seasonal and has difficulties in conventional breeding due to dormancy, it must be conserved through in vitro tissue culture for a steady supply throughout the year in massive amounts for agricultural demand. A maximum number of 19.28 ± 0.37 shoots has been obtained in MS medium fortified with 6-Benzylaminopurine, Kinetin, and Naphthalene acetic acid. The genetic uniformity of the plants has been studied through Start Codon Targeted Polymorphism. Therefore, this study must help meet the need for essential phytoactive compounds through a simple, validated, and reproducible plant tissue culture protocol throughout the year.

Joint Action of Trichoderma atroviride and a Vegetal Derived-Protein Hydrolysate Improves Performances of Woodland Strawberry in Italy

Woodland strawberry (Fragaria vesca L.) is an underutilized plant species that could benefit from the use of biostimulants to improve crop productivity and fruit quality. The scope of the present study was to appraise the influence of two biostimulants (Trichoderma atroviride and plant protein hydrolysate)—used either alone or combined—on the plant performance and economic profitability of two woodland strawberry genotypes (‘Alpine’ and ‘Regina delle Valli’). Overall, data showed that ‘Alpine’ had the highest productive performances, whereas ‘Regina delle Valli’ revealed the highest fruit qualitative traits. T. atroviride inoculation and V-PH application significantly boosted plant marketable yield (+20.5% for T. atroviride and +12.9% for V-PH), total sugars (+1.9% for Trichoderma and +1.4% for V-PH) and anthocyanins (+14.1% for T. atroviride and +9.8% for V-PH) compared to non-treated plants. Plants supplied with both biostimulants had a higher marketable yield (+34.8%), mean fruit weight (+6.0%), fruit dry matter (+13.8%), total sugars (+3.5%), ascorbic acid (+12.7%), flavonoid (+26.3%) and anthocyanins (+29.9%) compared to non-treated plants. Furthermore, our study revealed that the highest fruit polyphenol concentration was recorded in both genotypes treated with the combination of biostimulants and in ‘Regina delle Valli’ sprayed with V-PH, whereas the highest antioxidant activity was found in ‘Regina delle Valli’ fruit when plants were supplied with both biostimulants. Our study pointed out that the application of microbial and non-microbial biostimulants, especially in combination, might be a useful strategy for improving the performances of underutilized species and, therefore, encouraging their cultivation, valorization and economic profitability (+6208.3 EUR /ha when plants were exposed to both biostimulants).

Assessing the Genetic Diversity of Wild and Commercial Feijoa sellowiana Accessions Using AFLPs

Feijoa sellowiana (O. Berg) is a broadly widespread fruit tree species at a very early stage of domestication. Although appreciated for its flavored berries rich in nutrients and nutraceuticals, and as an ornamental plant, feijoa is still considered an underutilized species and little information is available about its genetic background, cultivar traceability and divergence. This study aimed to investigate, for the first time, the genetic diversity of feijoa through the application of AFLPs. Specifically, twenty cultivars from different countries and six wild types (WTs) from their area of origin (Misiones, Argentina) were analyzed. The AFLPs proved to be informative, revealing the values of the percentage of polymorphic loci (PPB), Nei’s genetic diversity (h), and the Shannon index (I) at 69.36%, 0.27, and 0.43, respectively, consistent with the average of long-lived perennial and outcrossing species. However, despite the limited number of WTs examined, the genetic variability (h) was higher (approximately 37%) within the six samples compared to cultivars. The population structure analysis identified three clusters, with WTs forming a separated cluster (III) as expected. Cultivars were divided into two clusters (I and II), with cluster I exhibiting a closer genetic proximity to WTs compared to cluster II. This finding was further confirmed using the UPGMA dendrogram based on Provesti distances. This work raised awareness of the genetic variability among the feijoa’s widespread cultivars and demonstrated that the limited genetic breeding programs over the last decades resulted in low diversity among them. Moreover, these results confirm the hypothesis that all varieties are derived from a single narrow ancestral population. The potential of this species is considerable and needs to be further investigated to exploit its peculiarities.

Evaluation of the nutritional properties and the antioxidant activity of rice bean (<i>Vigna umbellata</i>) seeds collected in Sơn La province

Vigna umbellata is an underutilized species in Vietnam, and more information about its nutritional values needs to be provided. This study investigated the nutritional composition and antioxidant activity of the seeds of 10 rice bean varieties collected from different districts of Son La province. Moisture content, ash and nutritional composition, including total protein, lipid, and carbohydrate were estimated. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, total phenolic content (TPC), and total flavonoid content (TFC) were carried out to assess the antioxidant activity. The results showed as follows: moisture content ranged from 0.44% to 1.23%, total ash values were obtained in the range between 38.46 and 45.72 mg/g DW, total protein content spread from 106.65 to 156.77 mg/g; total lipid varied from 7.37 to 11.90 mg/g, and total carbohydrate dominated the nutritional profile with 786.10 to 834.73 mg/g. Remarkably, there were significant differences (p < 0.05 using ANOVA) among the rice bean species from various districts of Son La province regarding moisture and protein content. Still, similar values were observed for other parameters (p > 0.05). The IC50 of the DPPH assay ranged from 259.06 µg/mL to 479.99 µg/mL, while the TPC varied from 2.85 to 4.98 mg GAE/g, and TFC values were in the range between 6.46 and 13.73 mg QE/g DW. Both TPC and TFC were significantly negatively correlated to antioxidant activity. This study is the first report on the nutritional compositions and antioxidant activity in the seeds of 10 rice bean varieties collected in Son La province. The findings in this study contribute to valorizing the V. umbellata species, which might be a promising plant as a low-priced staple food for ethnic people in mountainous regions.

Formulation of children's nutrient-dense recipes from Adansonia digitata pulp and Ocimum gratissimum leaves in North Benin

Neglected and underutilized species (NUS) can play an important role in nutrition and food security. This study aimed to promote the consumption of two NUS, Ocimum gratissimum and Adansonia digitata, by improving recipes that are traditionally consumed by children aged 6–23 months in the Atacora department. Two (02) improved recipes were formulated: baobab pulp-enriched porridge (BPP) and egusi sauce with african basil leaves and red palm oil (ESBR). Formulation was initially carried out using MINITAB 19 mixing software. For each recipe, two factors (two main ingredients of the recipe) were considered for the formulation. The optimized recipes were then tested through a hedonic test with a sample of 66 children in three (03) communes. The nutritional densities of accepted recipes were then determined. Results showed that most of the children liked both recipes formulated. The energy densities of both recipes (1.24 ± 0.03 kcal and 1.89 ± 0.05 kcal/g respectively for BPP and ESBR) met the recommended standards. As for nutritional densities, only iron recommended density was achieved for BPP (4.86 ± 0.12 mg/100 kcal) while for ESBR, in addition to iron density, zinc and vitamin A densities were also achieved. The BPP recipe covered good levels of daily nutritional requirements for one single feed for iron (46.42%) and energy (13.49%) considering the amount consumed by children, whereas the ESBR recipe covered levels of 26.61% for energy, 399.32% for vitamin A, 94.11% for iron and 17.44% for zinc. A better valorization of these species through cooking demonstration sessions addressed to mothers is necessary to ensure the consumption of recipes formulated by communities in general and children in particular.

Study on production and utilization of minor millets in Madhya Pradesh

Neglected and underutilized species (NUS) worldwide are gaining attention as they are suited to sustainable food systems offering food and nutritional security to people in marginal regions. Minor millets are one such group of crop species that can be utilized to supplement the cereal-centric diet. But they are largely neglected in terms of research and development, policy support, and consumer demand. Present study was carried out during 2020 in selected districts of Madhya Pradesh to explore the production, consumption and utilization of minor millets and to identify the problem faced by the farmers at the field level. Farmers grow local varieties of millets with poor management under rainfed conditions. The price received by the farmers vary widely, although it is economically viable. Most sample farmers were unaware of the possibility of processing the grain into various products that suit consumer demand and fetch better prices. They perceive the unavailability of high-yielding variety as the major production constraint. The revival of millets requires a multidimensional approach involving government support, research and development, awareness campaigns, and consumer demand.

Shaping the Future of Coffee: Climate Resilience, Liberica's Rise, and By-Product Innovation-Highlights from the International Coffee Convention 2023 (ICC2023).

The International Coffee Convention 2023 comprehensively addressed the contemporary challenges and advancements in the coffee industry, emphasizing sustainability, health, and innovation. This convention gathered experts and stakeholders to explore diverse aspects of coffee, ranging from the potential of underutilized species like Coffea liberica in terms of climate resilience to the innovative use of coffee by-products. The convention featured presentations and discussions, employing both empirical research and analytical reviews to explore various topics, including the health benefits of coffee, the advancements in traceability and authentication methods, and the impact of global regulatory changes on coffee production and trade. The key findings highlighted the importance of biodiversity in coffee production as a response to climate change, the significant health benefits and sustainability potential of coffee by-products, and the evolving landscape of coffee consumption patterns driven by technological innovations. The convention also stressed the need for alignment in global coffee trade regulations, particularly concerning deforestation and traceability. The 2023 convention underscored the complexity and interconnectivity of the coffee industry's challenges and opportunities. It concluded with a forward-looking perspective, emphasizing the need for continued research, sustainable practices, and collaborative efforts to shape the future of the coffee industry. The community is looking forward to furthering these discussions at the next International Coffee Convention in 2024.

Traditional uses, Phytochemistry, Pharmacology, and Toxicology of the Genus Artemisia L. (Asteraceae): A High-value Medicinal Plant.

Biologically active secondary metabolites, essential oils, and volatile compounds derived from medicinal and aromatic plants play a crucial role in promoting human health. Within the large family Asteraceae, the genus Artemisia consists of approximately 500 species. Artemisia species have a rich history in traditional medicine worldwide, offering remedies for a wide range of ailments, such as malaria, jaundice, toothache, gastrointestinal problems, wounds, inflammatory diseases, diarrhoea, menstrual pains, skin disorders, headache, and intestinal parasites. The therapeutic potential of Artemisia species is derived from a multitude of phytoconstituents, including terpenoids, phenols, flavonoids, coumarins, sesquiterpene lactones, lignans, and alkaloids that serve as active pharmaceutical ingredients (API). The remarkable antimalarial, antimicrobial, anthelmintic, antidiabetic, anti-inflammatory, anticancer, antispasmodic, antioxidative and insecticidal properties possessed by the species are attributed to these APIs. Interestingly, several commercially utilized pharmaceutical drugs, including arglabin, artemisinin, artemether, artesunate, santonin, and tarralin have also been derived from different Artemisia species. However, despite the vast medicinal potential, only a limited number of Artemisia species have been exploited commercially. Further, the available literature on traditional and pharmacological uses of Artemisia lacks comprehensive reviews. Therefore, there is an urgent need to bridge the existing knowledge gaps and provide a scientific foundation for future Artemisia research endeavours. It is in this context, the present review aims to provide a comprehensive account of the traditional uses, phytochemistry, documented biological properties and toxicity of all the species of Artemisia and offers useful insights for practitioners and researchers into underutilized species and their potential applications. This review aims to stimulate further exploration, experimentation and collaboration to fully realize the therapeutic potential of Artemisia in augmenting human health and well-being.

Livelihood & Climate Change Implication of In-situ Conservation Strategies for Punica granantum and Syzygium cumini in Sunni Tehsil, Shimla H. P. India

Sustainably harvesting and selling wild fruits can offer rural communities an economical source of income while concurrently safeguarding ecosystems. This study aims for the global promotion of wild edible fruits (WEFs) in the rural areas of Sunni Nagar Panchayat, located in the Shimla district of Himachal Pradesh. This study primarily focuses on comprehensively documenting the utilization of wild edible fruits by local inhabitants through a structured survey and in-depth interviews. Employing a multistage random sampling technique, five villages—Ambari, Kadog, Kamla dugri, Kalwi, and Basantpur—were chosen based on the prevalence of Punica granatum and Syzygium cumini. This research underscores the economic advantages of collecting WEFs for rural communities, providing them with affordable and easily accessible food sources. Additionally, it promotes public awareness, community-based management, and research on indigenous fruits to diversify diets and contribute to the overall well-being of these communities.
 Pomegranate and Jamun (local names referring to the botanical species of Punica granatum and Syzygium cumini, respectively) trees play crucial roles in ecological conservation by offering habitat, shelter, and promoting biodiversity, while also combating soil erosion. However, escalating threats such as population growth, forest fires, urbanization, and timber extraction endanger this rich biodiversity. To counter plant extinction risks, a strategic approach to forest conservation is imperative, emphasizing sustainable collection and trade of underutilized species beneficial for food, medicine, and income. Climate change poses challenges to pomegranate production, impacting orchards with temperature fluctuations, altered precipitation, extreme weather, and pests. Adaptation measures, including irrigation, pest management, and diversification, are crucial. Preserving wild pomegranate and Jamun demands habitat protection, restoration, and sustainable agriculture through agroforestry research, community engagement, and supportive policies. Integrating traditional knowledge with modern conservation ensures a comprehensive approach, actively preserving genetic diversity, ecological balance, and cultural relevance through multi-dimensional conservation efforts for Pomegranate and Jamun.

Contribution of pollinators to production and reproduction of neglected and underutilized food species in family farming systems in Patagonia

ABSTRACT Many of the species used in family farming systems (FFS) in Patagonia belong to the group known as neglected and underutilized species (NUS), which promote biocultural diversity and contribute to food sovereignty. Due to the scarce attention paid to NUS by agronomic sciences, little is known about their biotic pollination, although it is a well-studied aspect for commercially important plants. We evaluate the contribution of animal pollination to NUS managed by Patagonian FFS. We present a literature review of the NUS used and aspects related to their pollination and floral visitors, for which 725 papers were reviewed. FFS use 426 NUS (414 angiosperms, 10 gymnosperms and 2 ferns). Most NUS (86.7%) depend to some extent on animal pollination, either for the production of fruits or seeds that are harvested for consumption (direct dependence, 35%) or for obtaining seeds (indirect dependence, 65%), which in many cases are necessary for reseeding. Gathering and cultivation are the most common management practices for these species. Hymenoptera and Diptera are the animals that contribute most to NUS biotic pollination. Our results show that animal pollination is a key contribution of nature to sustain cultural aspects and food sovereignty of FFS in Patagonia.

Supercritical CO2 for efficient extraction of high-quality starfish (Asterias rubens) oil

The growing demand for healthy marine lipids and the unmatched supply demand required diversification of their sources. Characterization of starfish oil has shown that it is a new possible source of marine lipids. However, optimized extraction conditions are needed to extract high quality oil. Hence, this study aimed to extract starfish oil using supercritical carbon dioxide (ScCO2) and optimize the extraction process using response surface methodology (RSM). Temperature, pressure, and ethanol flow rate were the most influential parameters for oil extraction. The RSM model predicted 19.40% and 25.40% for extraction yield and phospholipids content respectively at 40 °C, 350 bar, and 2.61 mL/min of ethanol flow rate. The result of validation extraction showed close agreement with the model. Moreover, the oils were characterized by fatty acid composition, peroxide value, free fatty acid, and tocopherol contents. The current study showed that high-quality lipids could be extracted from underutilized species using ScCO2 extraction.

Assessing the economic, social and environmental potential of wild and domesticated olive growing options in a far Western District of Nepal

CONTEXTClimate change and extreme weather events undermine smallholder household food and income security in many rural areas of developing countries. Nature-based solutions and the use of climate resilient and nutrient dense Neglected and Underutilized Species into the local farming system have been claimed to sustainably help farmers adapt to climate change and to manage risk more effectively. OBJECTIVEThis paper investigated the potential implementation of three options based on the collective cultivation and management of olive groves and wild forests for production purposes in a mountain region of Nepal. The analysis was aimed at assessing the potential benefits of such strategy in comparison with the status-quo. METHODSThe study conducted an ex-ante simulation exercise for evaluating three options: 1) 1500 ha of new and restored wild forests; 2) 500 ha of olive groves; and 3) A combination of option 1 and 2. We calculated the financial, economic, social, and environmental costs and benefits of such options using agricultural and economic data collected from representative households identified from a sample of 31 small, 9 medium, and 3 large farm households. To conduct the economic, social and environmental analysis (ESE), we calculated the Social Return on Investment and we integrated it into the economic analysis. Then we compared the results to assess which was the most profitable option. RESULTS AND CONCLUSIONSThe results of the analysis showed that all three proposed options are stable with an Internal Rate of Return always above 27% with values over 100%. The greatest increase in terms of NPV is observed when shifting from the financial to the ESE analysis in options 1, 2, and 3 (+397.5%, +132.0%, +187.2% respectively) as the benefits increase more than the costs. The results further suggested that the integrated option is the most profitable one also in terms of labour generation. Indeed, we observed a substantial increase in the Return to Labour indicator especially in the ESE analysis (from 1.9 in the financial analysis to 2.8 in the economic analysis to 6.0 in the ESE analysis). SIGNIFICANCEThis study provides evidence of the potential that similar strategies based on the use of local resources and species, if properly designed, implemented and managed, may have to restore the ecosystem and enhance the livelihoods of local communities. The most innovative element of this study is the proposed ESE analysis, which takes into account the social and environmental dimension besides the economic one.