Understanding Of PD Research Articles

Mass spectrometry‐based proteomics for biomarker discovery in the Drosophila model of Parkinson's disease

AbstractParkinson's disease (PD) is the second most prevalent neurodegenerative disorder worldwide. Despite extensive research, the etiology of both familial and sporadic PD remains unclear. While most PD cases are sporadic, a significant minority are linked to genetic mutations, notably in the synuclein‐alpha (SNCA) and leucine‐rich repeat kinase 2 (LRRK2) genes. Animal models, such as Drosophila melanogaster (D. melanogaster), enable detailed study of these genetic mutations and their neurotoxic effects. Recent advancements in mass spectrometry‐based proteomics have enhanced our understanding of PD by facilitating comprehensive analysis of protein expression and interactions in mutant and wild‐type organisms, potentially revealing novel therapeutic targets. This review highlights the pivotal role of mass spectrometry‐based proteomics in advancing PD research, emphasizing the contributions of D. melanogaster models in identifying potential biomarkers.

Evaluation of Knowledge and Attitudes of the Population of Tabuk City Regarding Parkinson's Disease: A Cross-Sectional Study.

The lack of awareness and information about PD may be a barrier to early diagnosis and the delivery of the best care to patients with the condition, given its rising prevalence. In order to determine the variables that are connected to these parameters, this study sought to ascertain the general public's knowledge and awareness of PD in Tabuk City. In Tabuk City, a cross-sectional demographic survey was carried out. A validated structured questionnaire was used to interview adult respondents by random sampling regarding specific knowledge, attitudes, and awareness related to Parkinson's disease. According to the density of the city, a total of 426 members of the general population were chosen at random and interviewed by skilled interviewers. Age and educational attainment were independently linked to PD awareness. Bachelor's degree subjects and those between the ages of 18 and 45 displayed a greater awareness of PD. Those above 60 and those between the ages of 46 and 60 lacked sufficient knowledge. The majority of participants demonstrated adequate understanding and awareness of PD in their respective occupations. Age, gender, occupation, and level of education were all adequately covered by knowledge and understanding of PD. To increase public knowledge, attitudes, and awareness of PD, however, suitable educational tactics and approaches targeting particular subgroups are required.

Parkinson Detection using Machine Learning Algorithms

Abstract: Parkinson’s Disease (PD is an irreversible, chronic, and progressive disorder, estimated to affect about ten million people worldwide affecting patient movement and feelings of motivation and reward. Motor symptoms of PD include tremor or shaking, which usually begins in one arm or hand, muscle rigidity or stiffness, and slowness, also referred to as bradykinesia. Signs and symptoms are unique to each person and the treatment should be tailored to each person’s individual needs. A recent survey of 492 people with PD found most believed self-monitoring would improve their understanding of PD, well-being, ability to cope, and communication with their health-care team. The overlap of symptoms with other neurological disorders leads to difficulties, delays, and errors in diagnosis, negatively impacting patients. A recent study of 1775 patients’ experiences of PD diagnosis identified the importance of early diagnosis. and found misdiagnosis occurred at first consultation for 15% of participants. This fallibility of clinical diagnosis is recognized and requires periodic diagnosis reviews. Participants are presented with three activities evaluating Patients Call Response, Fine Motor Movements and Memory. To evaluated Patients, Name Call Response, the application uses a Chatbot to communicate with the patient, and tries to ask questions by calling his name. On successful response a series of other questions are asked on patients likes and dislikes. Fine Motor Deficit is evaluated based on a Finger Tapping Task, the patient as to tap on two buttons shown on the phone screen alternatively. The data is collected after 2 minutes on Tapping and compared with threshold set by the Normal Group. The Memory Activity measures the time taken by the patient to solve the puzzle by finding the corresponding images. Patients are shown images of fruits that are illuminated one at a time and then asked to find the same in the grid.

LINC00667 regulates MPP+ -induced neuronal injury in Parkinson's disease.

ObjectiveParkinson’s disease (PD), also known as paralysis tremor, is a chronic disease of the central nervous system. It has been reported that hepatocyte nuclear factor 4 alpha (HNF4A) is upregulated in PD, but its specific function has not been well explored.MethodsWe established an in vitro PD model in SH‐SY5Y cells stimulated with 1‐methyl‐4‐phenylpyridinium (MPP+). Meanwhile, the effect of HNF4A on MPP+‐treated SH‐SY5Y cell behavior was monitored by functional assays. Mechanism assays were conducted to verify the relationship among LINC00667/miR‐34c‐5p/HNF4A. Rescue experiments validated the regulatory mechanism in PD model.ResultsThe results revealed that depletion of HNF4A suppressed cell cytotoxicity and apoptosis caused by MPP+. Knockdown of HNF4A recovered MPP+‐stimulated oxidative stress and neuroinflammation. Mechanically, HNF4A was targeted and inhibited by miR‐34c‐5p. Furthermore, we found that LINC00667 positively modulated HNF4A expression via sequestering miR‐34c‐5p in MPP+‐stimulated SH‐SY5Y cells. Interestingly, the data indicated that HNF4A could transcriptionally activate LINC00667 expression. Rescue experiments presented that miR‐34c‐5p interference or HNF4A overexpression could mitigate the effects of LINC00667 knockdown on cell viability, cytotoxicity, cell apoptosis, oxidative stress, and neuroinflammation in MPP+‐treated SH‐SY5Y cells.ConclusionOur study first proved LINC00667, miR‐34c‐5p, and HNF4A constructed a positive feedback loop in MPP+‐treated SH‐SY5Y cells, enriching our understanding of PD.

Caring for persons with Parkinson’s disease in care homes: Perceptions of residents and their close relatives, and an associated review of residents’ care plans

Abstract Through qualitative in-depth interviews, we collected the views of persons with Parkinson’s disease (pwPD) and their close relatives in care homes to establish their collective views of the effectiveness of care. We also reviewed the corresponding care plans. Drawing on these two forms of data collection, we compared similarities and differences between the qualitative interview data and the care plan analysis to elaborate on the experience of residential care for pwPD. Close relatives of care home residents can be a fruitful source of information for care home staff, throughout the care planning process, especially in relation to the specific needs of a pwPD. Although health and social policy advocate active collaboration between people with long-term conditions, their families, and their formal carers, there is limited evidence of such collaboration in the data examined here. There is an apparent shortfall in the knowledge and understanding of PD among care home staff. There are important pragmatic (e.g. drug administration) as well as psycho-social reasons for flexibility in routine care provision to meet the dynamic needs of pwPD. The findings here support the need for further, larger scale research into the quality of care for pwPD who are care home residents.

4.003 Towards the molecular understanding of PD by applying proteomic approaches

4.003 Towards the molecular understanding of PD by applying proteomic approaches

Production of light mesons in P + D reactions

Recent progress in our understanding of pd → 3 Heπ 0 up to 2.5 GeV is reviewed. The main contributions are known but the calculations do not reproduce details of the observables even in the Δ peak. The experimental data on neutral meson production at threshold up to the Φ(1020) is discussed. The measured high counting rate of η's provides a nice opportunity to study rare and forbidden η decays by the easy 3He tagging.