Unconventional Patterns Research Articles

Direct patterning of liquid materials on flat and curved substrates using flexible molds with through-hole and post arrays.

Liquids undergo continuous deformation in the presence of external shear stresses; however, they are pinned between structures owing to their viscosity. Therefore, reshaping the liquids using their intrinsic material properties and structural interfaces is possible. In this study, we used the template-guided forming (TGF) method to reshape and produce oil patterns on flat and curved substrates. To produce oil patterns, we developed two oil patterning methods: direct heating-based oil patterning (DHOP) and solvent evaporation-based oil patterning (SEOP), which were characterized using various oils and solvents. To overcome the limitation of relying solely on liquid patterning that undergoes complete evaporation, we successfully fabricated liquid films using oil and nonpolar organic solvents that exhibit long-term stability. Therefore, achieving durability and control over the film thickness using nonpolar organic solvents has great potential for future applications in microfluidics. Furthermore, we demonstrated that the SEOP method in conjunction with TGF can produce various and unconventional patterns of an organic photoresist (SU-8), which cannot be produced through standard photolithography. Hence, we conclude that the proposed TFG-based oil pattering methods could be highly useful for producing unconventional and unprecedented patterns on flat and curved substrates for various applications, including microelectronics, optics, filtration and separation, biomedical engineering, and nanotechnology.

Rethinking citations: proposing a new model for analyzing stance and rhetorical functions of citations in interdisciplinary discourse

ABSTRACT The current study investigates citation practices in publications published in the journal Global Environmental Change. Twenty papers were selected to cover the period from 1990 to 2010. The model was applied to the selected papers, which included hundreds of in-block citations, to identify categories at different levels and compare clause- and block-level findings for each citation. The proposed model is considered a triangular system that integrates as well as aligns the chosen categories of the functions, stance, and form of citation. A comparison between the block-level analysis of citation functions and the clause-level analysis of stance categories demonstrates how citations work and their role in citation texts. By combining functional categories and stance, we gain an understanding of both conventional and unconventional patterns of citation. The article argues that starting with the block-level analysis, as a recently discovered level of analysis, is most useful for understanding students’ awareness of the block and how it affects the execution of citation practices by researchers or writers—specifically, the position of each citation. The suggested system can be extended to interdisciplinary discourse to investigate heterogeneity in citation practices.

Novel insight into miRNA biology and its role in the pathogenesis of systemic lupus erythematosus.

MicroRNAs(miRNAs) have emerged as key regulators that control and influence gene expression as well as multiple biological processes depending on their potential binding sites in human-protein coding genes and other unconventional patterns, including coding for peptides, activating Toll-like receptors as a ligand, and other manners. Accumulating evidence has demonstrated that microRNA expression is tightly regulated during phases of development, differentiation, and effector functions of immune cells, immunological disorders of systemic lupus erythematosus (SLE). This review outlines the biogenesis of miRNAs and their unconventional functions as well as underlying cellular and molecular mechanisms. It then summarizes our current knowledge about how the biogenesis of miRNAs is regulated. Moreover, an overview was provided concerning the role of abnormal expression of miRNAs in lupus immune cells. In particular, we will shed some light on the recent advances in the role of miRNAs and exosome-derived miRNAs in immunological and epigenetic pathways in the pathogenesis of SLE.

Unconventional patterns on surfaces

Unconventional patterns on surfaces

Unconventional patterns on surfaces

We present a unified method to meshing surfaces with unconventional patterns, both periodic and aperiodic. These patterns, which have so far been studied on the plane, are patterns comprising a small number of tiles, that do not necessarily exhibit translational periodicity. Our method generalizes the de Bruijn multigrid method to the discrete setting, and thus reduces the problem to the computation of N -Directional fields on triangle meshes. We work with all cases of directional symmetries that have been little studied, including odd and high N. We address the properties of such patterns on surfaces and the challenges in their construction, including order-preservation, seamlessness, duality, and singularities. We show how our method allows for the design of original and unconventional meshes that can be applied to architectural, industrial, and recreational design.

Effect of zinc ion concentration on crosslinking of carboxymethyl cellulose sodium-fumaric acid composite hydrogel

Anionic polysaccharides are known to react in unconventional patterns with di-valent metal ions to form 3D-structured hydrogels. However, little is known about the influence of divalent ions concentration on hydrogel formation. Herein, carboxymethyl cellulose based hydrogels crosslinked with zinc ions (Zn2+) and fumaric acid are synthesized and characterized for swelling, structure, morphology and thermal properties, and the effect of Zn2+ concentration on hydrogel properties is investigated. It was found that increasing Zn2+ to 0.5 M enhances gel fraction and water absorbency, at which super-absorbent yet stable, crystalline (79% crystallinity index) FA-0.5 exhibiting water-absorbency (2259%) and stability toward water (51%) and heat (−3.17 mW and 0.9% degradation at 50 °C) is formed. The results reveal that crosslinking is influenced by Zn2+ concentration and interaction with other hydrogel components. The hydrolytic and thermal properties of FA-0.5 shows potential as a stable biomaterial.

Kinetochore-independent mechanisms of sister chromosome separation.

Although kinetochores normally play a key role in sister chromatid separation and segregation, chromosome fragments lacking kinetochores (acentrics) can in some cases separate and segregate successfully. In Drosophila neuroblasts, acentric chromosomes undergo delayed, but otherwise normal sister separation, revealing the existence of kinetochore- independent mechanisms driving sister chromosome separation. Bulk cohesin removal from the acentric is not delayed, suggesting factors other than cohesin are responsible for the delay in acentric sister separation. In contrast to intact kinetochore-bearing chromosomes, we discovered that acentrics align parallel as well as perpendicular to the mitotic spindle. In addition, sister acentrics undergo unconventional patterns of separation. For example, rather than the simultaneous separation of sisters, acentrics oriented parallel to the spindle often slide past one another toward opposing poles. To identify the mechanisms driving acentric separation, we screened 117 RNAi gene knockdowns for synthetic lethality with acentric chromosome fragments. In addition to well-established DNA repair and checkpoint mutants, this candidate screen identified synthetic lethality with X-chromosome-derived acentric fragments in knockdowns of Greatwall (cell cycle kinase), EB1 (microtubule plus-end tracking protein), and Map205 (microtubule-stabilizing protein). Additional image-based screening revealed that reductions in Topoisomerase II levels disrupted sister acentric separation. Intriguingly, live imaging revealed that knockdowns of EB1, Map205, and Greatwall preferentially disrupted the sliding mode of sister acentric separation. Based on our analysis of EB1 localization and knockdown phenotypes, we propose that in the absence of a kinetochore, microtubule plus-end dynamics provide the force to resolve DNA catenations required for sister separation.

Influences of Printing Pattern on Mechanical Performance of Three-Dimensional-Printed Fiber-Reinforced Concrete.

Underperformed interfacial bond and anisotropic properties are often observed in three-dimensional-printed concrete, where the printing pattern is unidirectional. Such issues could be potentially alleviated by replicating microstructures of natural materials or applying different architectures, where printed layers are arranged into unique and unconventional patterns. Furthermore, the quest to develop printing methods for highly complex or self-support concrete architecture could benefit from these nature-inspired patterns. In this work, the influences of different architectural arrangements of layers on mechanical properties of hardened concrete on compressive and flexural strengths are investigated. Specifically, unidirectional (0°), cross-ply (0°/90°), quasi-isotropic (0°/ ± 45°/90°), and helicoidal patterns (with pitch angles of 10°, 20°, and 30°) are used to create unidirectional, bidirectional, and multidirectional layers in printed objects without and with 0.75% by volume of 6 mm-long steel fibers. The experimental results demonstrate considerable improvements in the flexural strengths of nontraditional specimens without steel fibers over the unidirectional control with a few exceptions. Among investigated patterns, the quasi-isotropic demonstrates significant influences in both compressive and flexural responses of printed concrete samples without steel fibers. The addition of steel fibers leads to noticeable improvement on both compressive and flexural strengths of samples in any pattern compared with their counterparts without fibers. Besides, the inclusion of steel fibers into unconventional layups (cross-ply, quasi-isotropic, and helicoidal patterns) leads to the alleviation of directional dependence of mechanical properties, which is a limitation of the unidirectional samples with fibers. Of all helicoidal patterns, the one with a 10°-angle layup is shown to be more beneficial to the flexural strength enhancement and damage resistance in bending. X-ray microcomputed tomography measurements are performed to visualize the direction and distribution of fibers.

Improvement of Trip Attraction Model in Surabaya by Considering Geographical Weighting of City Centre Activity Function

It is challenging to have a trip attraction model that fits Surabaya's surveyed data due to unclear city centre structure. These include the centrum of concentric, corridors of sectoral, or several centres of multiple-nuclei structures. Also, the layout of residential areas has unconventional patterns. This is because the planned housing development area is wrongly inserted on kampong and sometimes lies in city centre. This paper examines the influence of single-centre districts, corridors, or multiple suburb centre structures on trip attraction. The analysis was conducted using origin-destination data from the household interview survey by The Transportation Board of Surabaya and several houses digitized from a relevant year's satellite image. The distance and position information was taken from the Google Earth application. The zonal analysis trip attraction model based on the sub-district zoning system was analysed using fixed trip production data and simulated independent variables. The independent variables included the zonal activity areas such as shops, offices, and industries in sub-district, while the dependent variables consisted of the straight distances from the sub-district to city centres. Several models were tested based on the dependent and independent variables. The results show that the combined zonal activity area and spatial variables have a stronger influence on zonal trip attraction than the conventional model using zonal labor and student variable, mainly based on the urban geographical pattern.

Listeners' adaptation to unreliable intonation is speaker-sensitive

Variable linguistic environments require the ability to quickly update expectations and behavior including speech comprehension. This adaptive capacity is key to understanding how listeners successfully recognize speaker intentions in light of the ubiquitous variability in speech. The present study investigates how listeners' real-time sentence comprehension adapts to speaker-specific prosodic variability. In two forced choice mouse tracking experiments, listeners had to identify a visual referent guided by pre-recorded instructions. When exposed to a speaker that uses unconventional pitch accent placement, listeners discard intonational information for that speaker, but keep using intonation to resolve the referential ambiguity for another speaker that places pitch accents conventionally. These results show for the first time that intonationally guided sentence comprehension adapts in a speaker-sensitive way. The study further provides valuable first insights into the temporal unfolding of this adaptation process. Listeners first attribute unconventional patterns to the context, thus discarding the informational value of intonation for both speakers. After sufficient evidence, however, listeners start attributing unexpected patterns to only the unconventional speaker. Materials, data, and scripts can be retrieved here: http://osf.io/fdpg4

Imaging the Cancer Immune Environment and Its Response to Pharmacologic Intervention, Part 1: The Role of 18F-FDG PET/CT.

Immunotherapy agents are now entering the clinic in a wide array of malignancies and have provided a valuable addition to the therapeutic armamentarium. These agents enhance the global immune response by modulating the tumor microenvironment but can lead to unconventional patterns of response, challenging the conceptual framework that imaging is a robust surrogate for therapeutic efficacy. There is also increasing evidence that an effective antitumor response requires a systemic immune response in primary and secondary lymphoid tissues. However, an enhanced systemic immune response can lead to disruption of immunologic hemostasis in healthy tissues, causing adverse events. Better understanding of the complex interplay between tumoral and systemic immune response has been provided through tissue and liquid biopsy. However, the applicability of these methods is constrained by the biologic, spatial, and temporal heterogeneity of the processes involved. There is a growing interest in molecular imaging of cell-specific lineage markers of the immune system using biomolecules. However, the ongoing role of the more widely available 18F-FDG PET/CT for response assessment is being recognized through ongoing refinement of interpretative guidelines and emerging evidence. These noninvasive methods provide insights into the biologic basis of the global immune response to maximize potential therapeutic benefit. In this review, we aim to provide an overview of the current status of 18F-FDG PET/CT in the monitoring of tumoral and systemic immune response. In a companion review, the role of other imaging probes that might complement 18F-FDG PET/CT will be discussed.

Analysis of Temporary Employment Company Business Environment

Analysis of Temporary Employment Company Business Environment

New insights into oyster high-resolution hinge growth patterns

While oyster shells are one of the most common mollusks used for the analysis of (paleo)environmental and (paleo)climatic records based on geochemical proxies, high-resolution growth rate changes still need to be determined. Promising previous works are restricted to small portions of shell sections due to difficulties in continuous growth increment revelation. Based on a mark and recapture experiment of Magallana gigas specimens reared in an intertidal area of Normandy (France) for 22 months, and a sclerochronological approach using cathodoluminescence microscopy, this study provides the longest high-resolution record of growth increments in oyster shells to date. Different growth patterns were identified likely related to the oyster age. After age 1 year, the formation of growth increments follows an expected tide-related model, leading to the mineralization of ~ 2 calcitic increments per day, together with growth rate changes at lunar and semi-lunar periodicities, and a seasonal trend with occasional growth breaks during winter when temperatures fall below ~ 6 °C. However, for oysters younger than 1 year, i.e., before reaching their sexual maturity, the growth increment analysis reveals unconventional patterns. In this case, oysters’ growth is associated with either a large number (~ 5) or less than one increment per day depending on the period. This pattern is also associated with frequent growth cessations, although the growth rate of the shell is high at this period. Our results illustrate that the high-resolution sclerochronological approach is required for accurate paleoenvironmental reconstructions based on oyster shells.

Voronoi-Like Grid Systems for Tall Buildings

In the context of innovative patterns for tall buildings, Voronoi tessellation is certainly worthy of interest. It is an irregular biomimetic pattern based on the Voronoi diagram, which derives from the direct observation of natural structures. The paper is mainly focused on the application of this nature-inspired typology to load-resisting systems for tall buildings, investigating the potential of non-regular grids on the global mechanical response of the structure. In particular, the study concentrates on the periodic and non-periodic Voronoi tessellation, describing the procedure for generating irregular patterns through parametric modelling and illustrates the homogenization-based approach proposed in the literature for dealing with unconventional patterns. To appreciate the consistency of preliminary design equations, numerical and analytical results are compared. Moreover, since the mechanical response of the building strongly depends on the parameters of the microstructure, the paper focuses on the influence of the grid arrangement on the global lateral stiffness, therefore on the displacement constraint, which is an essential requirement in the design of tall buildings. To this end, five case studies, accounting for different levels of irregularity and relative density, are generated and analyzed through static and modal analysis in the elastic field. In addition, the paper focuses on the mechanical response of a pattern with gradual rarefying density to evaluate its applicability to tall buildings. Displacement‐based optimizations are carried out to assess the adequate member cross sections that provide the maximum contribution in restraining deflection with the minimum material weight. The results obtained for all the models generated are compared and discussed to outline a final evaluation of the Voronoi structures. In addition to the wind loading scenario, the efficiency of the building model with varying density Voronoi pattern, is tested for seismic ground motion through a response spectrum analysis. The potential applications of Voronoi tessellation for tall buildings is demonstrated for both regions with high wind load conditions and areas of high seismicity.

Surface structures with unconventional patterns and shapes generated by femtosecond structured light fields

We present an investigation on ultrashort laser surface structuring with structured light fields generated by various q-plates. In particular, q-plates with topological charges q = 1, 3/2, 2, 5/2 are used to generate femtosecond (fs) vector vortex beams, and form complex periodic surface structures through multi-pulse ablation of a solid crystalline silicon target. We show how optical retardation tuning of the q-plate offers a feasible way to vary the fluence transverse distribution of the beam, thus allowing the production of structures with peculiar shapes, which depend on the value of q. The features of the generated surface structures are compared with the vector vortex beam characteristics at the focal plane, by rationalizing their relationship with the local state of the laser light. Our experimental findings demonstrate how irradiation with fs complex light beams can offer a valuable route to design unconventional surface structures.

Evaluation criteria for tumor efficacy in the times of immunotherapy

With the advent of the times of immunotherapy and the emergence of a variety of unconventional response patterns such as delayed response and pseudo-progressive disease, a variety of immune-related efficacy evaluation criteria such as immune-related response criteria, immune-related response evaluation criteria in solid tumor and immune response evaluation criteria in solid tumor have been proposed successively. The evaluation principles and judgment results of these criteria are distinctly different from the traditional response evaluation criteria. In particular, the newly proposed immune response evaluation criteria in solid tumor introduces two new concepts of unconfirmed progressive disease and confirmed progressive disease and provides a new response evaluation model for solid tumors, which is expected to provide solutions to some of the most urgent clinical problems under the times of cancer immunotherapy. Key words: Neoplasms; Immunotherapy; Response evaluation; Immune response evaluation criteria in solid tumors

Post- progression treatment patterns in advanced lung cancer patients treated with nivolumab.

e21070Background: In lung cancer patients receiving immunotherapy, radiographic disease progression (PD) has poor correlation with overall survival (OS) due to unconventional response patterns. The...

Do immune checkpoint inhibitors need new studies methodology?

Immune checkpoint inhibitors (ICI) have widely reshaped the treatment paradigm of advanced cancer patients. Although multiple studies are currently evaluating these drugs as monotherapies or in combination, the choice of the most accurate statistical methods, endpoints and clinical trial designs to estimate the benefit of ICI remains an unsolved methodological issue. Considering the unconventional patterns of response or progression [i.e., pseudoprogression, hyperprogression (HPD)] observed with ICI, the application in clinical trials of novel response assessment tools (i.e., iRECIST) able to capture delayed benefit of immunotherapies and/or to quantify tumor dynamics and kinetics over time is an unmet clinical need. In addition, the proportional hazard model and the conventional measures of survival [i.e., median overall or progression free survival (PFS) and hazard ratios (HR)] might usually result inadequate in the estimation of the long-term benefit observed with ICI. For this reason, innovative methodologies such as milestone analysis, restricted mean survival time (RMST), parametric models (i.e., Weibull distribution, weighted log rank test), should be systematically investigated in clinical trials in order to adequately quantify the fraction of patients who are "cured", represented by the tails of the survival curves. Regarding predictive biomarkers, in particular PD-L1 expression, the integration and harmonization of the existing assays are urgently needed to provide clinicians with reliable diagnostic tests and to improve patient selection for immunotherapy. Finally, developing original and high-quality study designs, such as adaptive or basket biomarker enriched clinical trials, included in large collaborative platforms with multiple active sites and cross-sector collaboration, represents the successful strategy to optimally assess the benefit of ICI in the next future.

‘nearerandnearerandNEARER’: Foregrounding effects of the unconventional capitalization in the experimental poetry of E. E. Cummings

AbstractMarked linguistic structures in E. E. Cummings’ poetry have long been an issue within literary criticism and stylistics. In this sense, critical approaches to Cummings’ style have dealt mainly with grammar, lexis and morphology, while only few works have examined his graphology extensively. Departing from these trends, in this paper I analyse the use of unorthodox capital letters in 96 of his experimental poems. My aim is twofold: to identify the processes present in Cummings’ unconventional use of capital letters and to determine the effects observable in such unusual patterns. The analysis reveals that the foregrounding of capitals is materialized by the insertion of initial caps, middle caps, final caps, all caps or mixed caps where these are not expected or required. It also suggests that these unconventional patterns mainly emphasize certain elements within the poems, produce iconic effects, generate wordplay and create chaotic scenes. To a lesser degree, they also schematize words, lines or a whole poem, and reproduce differences in the tone of some poetic voices or depict elements that are capitalized in real life.

Unconventional patterns in the experimental poetry of E. E. Cummings: A stylistic approach to punctuation marks

For some time scholars have examined unconventional linguistic patterns in E. E. Cummings’ poetic style. Of all the aspects under consideration, it is grammar, lexis and morphology that have been most widely researched, while only a small number of studies have looked at graphology or, least of all, punctuation. This article is a contribution to the latter research field, and is aimed at developing a systematic approach to the use of punctuation marks in E. E. Cummings’ experimental poetry. I deal with two fundamental research questions: What foregrounding patterns are present in E. E. Cummings’ unorthodox use of punctuation marks, and what effects derive from his singular use of marks? Using 157 experimental poems as a corpus, I identify any instances of unconventional punctuation and classify the different devices that break with convention, determining the meaning implications (if any) that derive from these particular uses. An in-depth analysis of these poems reveals that there are three basic unconventional devices in Cummings’ use of punctuation marks (substitution, omission and insertion) and that these help Cummings to achieve a variety of purposes: emphasize certain elements within the poem, shift the tempo of the lines, create chaotic scenes, produce iconic effects, schematize any unit within the poem, omit letters and words, signal heteroglossia, indicate imperative voice, articulate the poem into different layers, create plays on words, and reproduce features of spoken language.