Umbrella Model Research Articles

Competence-Based Trainings for Psychological Treatments - A Transtheoretical Perspective.

Although in most countries psychotherapy trainings focus on one treatment orientation, such an approach is associated with systematic shortcomings. The priorities from teaching one theoretical framework should be moved to a more rigorous orientation in science and evidence-based practice, and to the needs of patients, even if strategies of different theoretical approaches need to be combined. We discuss whether competence-based trainings in psychological treatments offer a better framework to facilitate the progress of psychological treatments to a professional academic discipline with transtheoretical exchange, and we provide an example of a transtheoretical education in the basic competences of psychological treatments. A transtheoretical education program requires an umbrella model for case formulation and a transtheoretical definition of intervention goals. We provide an adaptation of the traditional model distinguishing vulnerability/resilience, exacerbation, and maintenance of clinical problems for case conceptualization. Dynamic network models offer a further perspective for developing modern, transtheoretical case formulations. Treatment methods should be better classified according to their transtheoretical goals, which offers opportunities to better compare or combine them. We report a case example of how to transform a general competence-based approach in the training of psychological treatments in the academic education system, which found exceptional acceptance from participating students. Thus, a rigorous competence-based approach to training early clinicians in applying psychological treatments helps to bridge the artificial divide between psychotherapeutic traditions. It also supports the evolution of psychological treatments into an academically robust and highly professional, integrative discipline.

Hepatic cholesterol biosynthesis and dioxin-induced dysregulation: A multiscale computational approach

Humans are constantly exposed to lipophilic persistent organic pollutants (POPs) that accumulate in fatty foods. Among the numerous POPs, dioxins, in particular 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), can impact several organ systems. While the hazard is clearly recognized, it is still difficult to develop a comprehensive understanding of the overall health impacts of dioxins. As chemical toxicity testing is steadily adopting new approach methodologies (NAMs), it becomes imperative to develop computational models that can bridge the data gaps between in vitro testing and in vivo outcomes. As an effort to address this challenge, we propose a multiscale computational approach using a “template-and-anchor” (T&A) structure. A template is a high-level umbrella model that permits the integration of information from various, detailed anchor models. In the present study, we use this T&A approach to describe the effect of TCDD on cholesterol dynamics. Specifically, we represent hepatic cholesterol biosynthesis as an anchor model that is perturbed by TCDD, leading to steatosis, along with alterations of plasma cholesterol. In the future, incorporating pertinent information from all anchor models into the template model will allow the characterization of the global effects of dioxin, which can subsequently be translated into overall – and ultimately personalized – human health risk assessment.

A major conformational change of N-terminal helices of Bacillus thuringiensis Cry1Ab insecticidal protein is necessary for membrane insertion and toxicity.

Pore forming toxins rely on oligomerization for membrane insertion to kill their targets. Bacillus thuringiensis produces insecticidal Cry-proteins composed of three domains that form pores that kill the insect larvae. Domain I is involved in oligomerization and membrane insertion, whereas Domains II and III participate in receptor binding and specificity. However, the structural changes involved in membrane insertion of these proteins remain unsolved. The most widely accepted model for membrane insertion, the 'umbrella model', proposed that the α-4/α-5 hairpin of Domain I swings away and is inserted into the membrane. To determine the topology of Cry1Ab in the membrane, disulfide bonds linking α-helices of Domain I were introduced to restrict their movement. Disulfide bonds between helices α-2/α-3 or α-3/α-4 lost oligomerization and toxicity, indicating that movement of these helices is needed for insecticidal activity. By contrast, disulfide bonds linking helices α-5/α-6 did not affect toxicity, which contradicts the 'umbrella model'. Additionally, Föster resonance energy transfer closest approach analyses measuring distances of different points in the toxin to the membrane plane and collisional quenching assays analysing the protection of specific fluorescent-labeled residues to the soluble potassium iodide quencher in the membrane inserted state were performed. Overall, the data show that Domain I from Cry1Ab may undergo a major conformational change during its membrane insertion, where the N-terminal region (helices α-1 to α-4) participates in oligomerization and toxicity, probably forming an extended helix. These data break a paradigm, showing a new 'folding white-cane model', which better explains the structural changes of Cry toxins during insertion into the membrane.

Theoretical analysis of principal components in an umbrella model of intraspecific evolution

Principal component analysis (PCA) is one of the most frequently-used approach to describe population structure from multilocus genotype data. Regarding geographic range expansions of modern humans, interpretations of PCA have, however, been questioned, as there is uncertainty about the wave-like patterns that have been observed in principal components. It has indeed been argued that wave-like patterns are mathematical artifacts that arise generally when PCA is applied to data in which genetic differentiation increases with geographic distance. Here, we present an alternative theory for the observation of wave-like patterns in PCA. We study a coalescent model – the umbrella model – for the diffusion of genetic variants. The model is based on genetic drift without any particular geographical structure. In the umbrella model, splits from an ancestral population occur almost continuously in time, giving birth to small daughter populations at a regular pace. Our results provide detailed mathematical descriptions of eigenvalues and eigenvectors for the PCA of sampled genomic sequences under the model. When variants uniquely represented in the sample are removed, the PCA eigenvectors are defined as cosine functions of increasing periodicity, reproducing wave-like patterns observed in equilibrium isolation-by-distance models. Including singleton variants in the analysis, the eigenvectors corresponding to the largest eigenvalues exhibit complex wave shapes. The accuracy of our predictions is further investigated with coalescent simulations. Our analysis supports the hypothesis that highly structured wave-like patterns could arise from genetic drift only, and may not always be artificial outcomes of spatially structured data. Genomic data related to the peopling of the Americas are reanalyzed in the light of our new theory.

ВПЛИВ ДЕМОГРАФІЧНОГО ФАКТОРУ НА СОЦІАЛЬНО–ЕКОНОМІЧНУ СТАБІЛЬНІСТЬ КИТАЮ

This research analyzes the demographic situation in China and its impact on socio-economic stability. Stable and balanced demographic reproduction is an important condition for the development of any economy, state and society. The implementation of effective social policy in China requires constant monitoring of the living standards of various social groups. Usually, in periods of deep and prolonged economic downturns, fiscal crisis, the state is forced to cut social spending, reduce the package of social services. Public services must justify budget expenditures in terms of costs and benefits. It is important that the monitoring and evaluation of budget expenditures is carried out efficiently and with minimal financial costs, so that the groups of the population who need it most can be identified. Scientific analysis of the Human Development Index and Population Reference Bureau data from 1950 to 2100 in China, Austria, Switzerland, the United States and the Netherlands shows a direct proportional economic dependence: the growth rate of gross domestic product (GDP) directly depends on the growth rate population and GDP growth per capita. Using the statistics recorded in the Seventh National Census of China, it is possible to trace the relationship between the level of socio-economic development of the country and its demographic situation. The author identifies 5 models of gender and age structure of the population: triangle, umbrella, helmet, spinning top, hat. Until 1979, China had an umbrella model with a high level of population growth. After the “one family – one child” policy, the situation has changed. The current demographic situation is reminiscent of the helmet model, the main characteristics of which show a decrease in the birth rate, a decrease in the working age population and an increase in the share of older people, which leads to excessive overload of the country's pension system. This pattern is described by the formula 4: 2: 1, which characterizes the situation when the Chinese government must, on the one hand, stimulate population growth, and on the other hand, be aimed at meeting the needs of older people and creating conditions for a new system of economic relations "longevity economy" or "silver economy".

The non-substitutability of local news?Advertising and the decline of journalism's umbrella market model

Abstract This article addresses the challenges facing local newspapers as the digital economy transitions to artificial intelligence (AI). We interview five CEOs of Scandinavian newspaper corporations, representing small, mid-sized, and large newspaper chains. The analysis focuses on three main factors emerging from the interviews – technological transformations, digital advertising markets, and corporate enrolment – and how they relate to business model disruption and the non-substitutability of local news. The analysis is set within the framework of the digital transformation, which, for the purpose of this study, we argue consists of two phases: getting online (until about 2014), and algorithmic adaptation (the introduction of programmatic advertising and audience metrics from about 2014). The analysis concludes that as non-substitutability is lost on the advertising side of the market, this challenges the umbrella model of newspaper publishing.

Two Coexisting Membrane Structures Are Defined by Lateral and Transbilayer Interactions between Sphingomyelin and Cholesterol.

The structure of fully hydrated bilayers composed of equimolar proportions of palmitoylsphingomyelin (PSM) and cholesterol has been examined by synchrotron X-ray powder diffraction and atomistic molecular dynamics (MD) simulations. Two coexisting bilayer structures, which are distinguished by the transbilayer phosphate-phosphate distance of coupled PSM molecules, are observed by diffraction at 37 °C. The MD simulations reveal that PSM molecules in the thicker membrane are characterized by more ordered, more extended, and less interdigitated hydrocarbon tails compared to those in the thinner membrane. Intermolecular hydrogen bonds further distinguish the two bilayer structures, and we observe the disruption of a sphingomyelin intermolecular hydrogen bond network induced by the proximity of cholesterol. Through an unsupervised clustering of interatomic distances, we show for the first time that the asymmetry of phospholipids is important in driving their interactions with cholesterol. We identify four distinct modes of interaction, two of which lead to the dehydration of cholesterol. These two modes of interaction provide the first description of precise physical mechanisms underlying the umbrella model, which itself explains how phospholipids may shield cholesterol from water. The most dehydrating mode of interaction is particular to the N-acylated fatty acid moiety of PSM and thus may explain the long-held observation that cholesterol preferentially mixes with sphingomyelins over glycerophospholipids.

Challenging the Cisgender/Transgender Binary: Nonbinary People and the Transgender Label

Interviews with 41 nonbinary individuals reveal a considerable amount of ambivalence among nonbinary people regarding transgender identification. There is also disagreement about which model of transgender legitimacy determines group membership: the binary and medicalized model or the umbrella model. Those who do not identify as transgender either do not consider themselves to be “trans enough” to claim group membership alongside trans men and trans women or otherwise consider their gender experience to be qualitatively different from the transgender experience. Meanwhile, those who do identify as transgender credit the umbrella model as authoritative while acknowledging that their claims to group membership are often resisted by those who uphold the binary transgender model. Finally, those who defy an easy yes/no transgender categorization schema qualify their transgender identification with admissions of doubt that they are really “trans enough” to claim the label or through linguistic practices that position themselves as tangentially transgender or as distinctly nonbinary transgender. This range of responses illuminates the diverse gender identities that coexist among nonbinary people that cannot be neatly sorted into a man/woman or cisgender/transgender binary. Research on the sociology of gender must expand beyond both of these binary frameworks to reflect the reality of gender diversity.

Development of integrated unit of agriculture and dietary education applying Drake's KDB model

이 연구는 Drake의 KDB 모형을 적용하여 농업과 식생활 영역의 통합적 접근 방법을 제안한 것이다. 최근의 학교 교육 경향은 교육과정의 충실도 관점에서 벗어나 교사가 교과서 없이 교육과정의 성취기준 중심으로 수업하는 창의적 접근 방법을 선호하고 있는데 그 과정 속에서 학문 영역 간 통합이 자연스럽게 일어나고 있다. 실과 역시도 통합을 지향하는 교과로 실과에서 최근 통합 대상으로 주목받고 있는 영역은 ‘농업’과 ‘식생활’이다. 이는 농업과 식생활이 ‘먹거리’를 매개로 하여 태생적으로 연결되어 있기 때문이다. 이에 이 연구에서는 KDB 모형을 ‘교육과정 분석’, ‘교육과정 조망’, ‘통합 단원 개발’의 3단계로 재구조화하여 농업과 식생활 영역의 통합적 접근 방법을 제안하였다. 교육과정 분석 단계에서는 성취기준을 ‘알 수 있는 것’ 과 ‘할 수 있는 것’으로 분석하여 계열화했다. 교육과정 조망 단계에서는 분석된 성취기준를 종횡으로 나누어 연결하는 내용 조망을 하였다. 통합 단원 개발 단계에서는 KDB 우산을 활용하여 간 학문적인 통합을 수행하였다. 이 연구의 결과로 기대되는 효과는 다음과 같다. 첫째, 농업과 식생활 영역 통합의 알고리즘을 제시함으로써 두 영역 간 통합 가능성을 촉진 시킬 것이다. 둘째, 농업과 식생활 영역 통합 수업의 예시를 제시함으로써 교육현장에서 농업과 식생활 영역 통합 수업의 실현 가능성을 높일 것이다. 셋째, 교육과정 중심의 통합 모형을 제시함으로써 실과교육 내 다른 영역 간 통합에 기여할 것이다.This study suggests an integrated approach to agricultural and dietary education applying Drake s KDB model. In recent years, the tendency of school education has shifted from the viewpoint of fidelity of curriculum, and teachers prefer creative approach to teaching curriculum achievement standards without textbooks. Recently, the areas of attention for integration are agriculture and dietary in practical arts. This is because agriculture and dietary are linked through eating . In this study, we proposed an integrated approach of agriculture and dietary education by restructuring KDB model into three steps of curriculum analysis, curriculum perspective, and integrated approach. First, in the curriculum analysis stage, the achievement standards were analyzed and sequenced. At the stage of the curriculum perspective, the analyzed achievement standards were divided into longitudinal and horizontal connections. In the integrated approach phase, the KDB umbrella model was used to perform interdisciplinary integration. The expected effects of this study are as follows. First, it will promote the integration possibility between the two areas by presenting the algorithm of integration of agriculture and dietary education. Second, it will increase the possibility of integrating agriculture and eating habits in the field by suggesting an example of integrated agriculture and eating habits. Third, by presenting an integrated model centered on curriculum, it can contribute to the integration of other areas within practical education.

SUCCESSFUL AGING A SELF-RELIANT UMBRELLA: DEFINING SUCCESSFUL AGING AMONG THE OLD-OLD (80+) IN SHANGHAI

The Chinese old-old (80+) population has steadily increased in recent years; however, limited studies have examined how they age. The purpose of this study is to explore how the old-old in urban China define successful aging. Guided by grounded theory, community-dwelling old-old individuals participated in semi-structured, in-depth interviews (N= 97). Participants identified self-reliance as the goal of successful aging, which was supported by four proactive behaviors, including physical activity, financial security, community connectedness, and willing acceptance of reality. These four proactive behaviors were conceptualized to constitute the ribs of an umbrella model offering a canopy to protect the pole of self-reliant successful aging. This study offers new insight in understanding dynamic and nuanced ways that the old-old in urban China age successfully and their valiant efforts to maintain an ideal status.

A Novel Convolutional Neural Network Architecture for SAR Target Recognition

Among many improved convolutional neural network (CNN) architectures in the optical image classification, only a few were applied in synthetic aperture radar (SAR) automatic target recognition (ATR). One main reason is that direct transfer of these advanced architectures for the optical images to the SAR images easily yields overfitting due to its limited data set and less features relative to the optical images. Thus, based on the characteristics of the SAR image, we proposed a novel deep convolutional neural network architecture named umbrella. Its framework consists of two alternate CNN-layer blocks. One block is a fusion of six 3-layer paths, which is used to extract diverse level features from different convolution layers. The other block is composed of convolution layers and pooling layers are mainly utilized to reduce dimensions and extract hierarchical feature information. The combination of the two blocks could extract rich features from different spatial scale and simultaneously alleviate overfitting. The performance of the umbrella model was validated by the Moving and Stationary Target Acquisition and Recognition (MSTAR) benchmark data set. This architecture could achieve higher than 99% accuracy for the classification of 10-class targets and higher than 96% accuracy for the classification of 8 variants of the T72 tank, even in the case of diverse positions located by targets. The accuracy of our umbrella is superior to the current networks applied in the classification of MSTAR. The result shows that the umbrella architecture possesses a very robust generalization capability and will be potential for SAR-ART.

Color brightness model and its imaging applications.

We have developed an empirical umbrella model for predicting the brightness of colors. It is based on a modified concept of the radiance factor. Instead of a single reference, i.e., white, for all colors, each color has its own reference color. Our model shows that, for near neutral colors, the boundary of the object-color solid accounts for most of the hue angles, except in the cyan-blue region, where the boundary has to be adjusted by experiments on color charts. We use an exponential function to extrapolate the umbrella to the colors of higher purity. The model is used to adjust the luminance of the Munsell colors to make them equally bright by compensating for the Helmholtz-Kohlrausch effect. It is also applied to real consumer images to reduce their brightness after boosting their color saturation. For both applications, the comparison images show that the model is quite effective.

A Self-Adaptive Umbrella Model for Vibration Analysis of Graphene.

The beam finite element and molecular dynamics models are two popular methods to represent the reaction of carbon-carbon bonds in graphene. However, the wrinkles and ripples in geometrical characteristics are difficult take into consideration. The out-planar mechanical properties are neglected in classical models of graphene. This paper proposes a self-adaptive umbrella model for vibration analysis of graphene. The parameters in the umbrella model are flexible to adapting the geometrical and material characteristics of graphene. The umbrella model consists of shell and beam elements. The honeycomb beam and planar shell model of graphene are included in the self-adaptive umbrella model as particular cases. The sensitivity analysis and results confirmed the rationality and feasibility of the self-adaptive umbrella model.

Spatially Resolving the Condensing Effect of Cholesterol in Lipid Bilayers

We study the effect of cholesterol on the structure of dipalmitoylphosphatidylcholine phospholipid bilayers. Using extensive molecular dynamics computer simulations at atomistic resolution, we observe and quantify several structural changes upon increasing cholesterol content that are collectively known as the condensing effect: a thickening of the bilayer, an increase in lipid tail order, and a decrease in lateral area. We also observe a change in leaflet interdigitation and a lack thereof in the distributions of dipalmitoylphosphatidylcholine headgroup orientations. These results, obtained over a wide range of cholesterol mole fractions, are then used to calibrate the analysis of phospholipid properties in bilayers containing a single cholesterol molecule per leaflet, which we perform in a spatially resolved way. We find that a single cholesterol molecule affects phospholipids in its first and second solvation shells, which puts the range of this effective interaction to be on the order of 1–2 nm. We also observe a tendency of phospholipids to orient their polar headgroups toward the cholesterol, which provides additional support for the umbrella model of bilayer organization.

Molecular Dynamics Investigation of the Ternary Bilayer Formed by Saturated Phosphotidylcholine, Sphingomyelin, and Cholesterol

Using the all-atom CHARMM36 force field, we have performed molecular dynamics simulations of the equimolar ternary dimyristoylphosphatidylcholine (DMPC)/ N-palmitoylsphingomyelin (PSM)/cholesterol bilayer. Analysis of its structural and kinetic properties has led us to the following conclusions. First, the DMPC/PSM/cholesterol bilayer features favorable interactions of cholesterol with DMPC and, particularly, PSM lipids, which are supported by hydrogen bonds. In contrast, the interactions between cholesterol molecules are strongly suppressed. Further analysis shows that about 60% of PSM molecules form hydrogen bonds with other PSM or cholesterol molecules and, to a lesser degree, with DMPC lipids. Second, local lipid packing around PSM molecules favors parallel or antiparallel alignments, resulting in the appearance of large anisotropic chain-like clusters formed by PSM lipids. In contrast, the distribution of DMPC lipids is closer to ideal, whereas the cholesterol distribution is sharply shifted toward small clusters or isolated molecules. Third, intermolecular interactions and mismatch in fatty acid tail lengths significantly slow down PSM rotational relaxation compared to that for DMPC, and PSM, but not DMPC lipids, tend to combine axial rotation with wobbling. Fourth, the analysis of cholesterol hydration indicates that lipid headgroups at most block about 30% of water molecules from reaching cholesterol, hinting at limited applicability of the umbrella model. Finally, we show that PSM and DMPC lipids predominantly exist in two equally probable conformational states, with and without a kink in their fatty acid tails. The appearance of these states is linked to the impact of cholesterol. Comparisons with previous studies allow us to delineate contributions of cholesterol, PSM, and DMPC to bilayer properties.

A theoretical prescription for Turkey higher education publicity: an umbrella model

The main objective of this study is to emphasize the importance of higher education publicity abroad. Data were obtained through a structured interview form with a qualitative research method, handled from the student’s perspective. The population of the study consisted of 325 students studying at the Centre of Ulutomer of Uludag University. One hundred students who had agreed to participate in the study were given an interview form, and 98 of them who returned the forms. Students at a rate of 70.58% stated that they had good conditions before arriving Turkey, where the 41.17% thought they had a good culture, while students at the rate of 11.76% stated that they thought they were conservative. After coming to Turkey, a large proportion of 80.76% of students stated that they are satisfied. As for education in the country, a rate of 30.46% indicated that education is not systematic. Of them, 59.18% said that they wanted to study in another country. By analyzing the definition, existing processes and models, it will be possible to develop a sub-structure for student development from abroad to public universities. With a model, based on educational philosophies and program development approaches, our country might be one that is preferred by students. It will be possible to achieve the desired goals such being a preferred country in higher education especially by those countries that have sent students abroad. By this way it will be possible to obtain all targets such as cultivation etc.

Resolving the 3D spatial orientation of helix I in the closed state of the colicin E1 channel domain by FRET. Insights into the integration mechanism

Current evidence suggests that the closed-state membrane model for the channel-forming domain of colicin E1 involves eight amphipathic α-helices (helices I–VII and X) that adopt a two-dimensional arrangement on the membrane surface. Two central hydrophobic α-helices in colicin E1 (VIII and IX) adopt a transmembrane location–the umbrella model. Helices I and II have been shown to participate in the channel by forming a transmembrane segment (TM1) in the voltage-induced open channel state. Consequently, it is paramount to determine the relative location and orientation of helix I in the two-dimensional arrangement of the membrane. A new, low-resolution, three-dimensional model of the closed state of the colicin E1 channel was constructed based on FRET measurements between three naturally occurring Trp residues and three sites in helix I, in addition to previously reported FRET distances for the channel domain. Furthermore, a new mechanism for the channel integration process involving the transition of the soluble to membrane-bound form is presented based on a plethora of kinetic data for this process.

Under the same umbrella: A model for knowledge and practice development

This article describes the development, organisation and significant elements of an Umbrella Model, which has been demonstrated to support knowledge and practice development in a large Danish hospital department. The aim is to inspire, support and guide implementation of the Umbrella Model in other settings. A shared theme overarching several minor projects is the core of the model and is exemplified in three completed programmes: Young people with cancer, Relatives and the cancer patient’s social network and Future cancer care – short but high quality. Commitment is achieved through close dialogue, supervision and programmes dealing with local needs and complex problems in clinical practice. The model unites clinical and academic competencies, has produced new knowledge and developed nursing care, and it has directed nurse leaders’ attention to the pivotal role they play in supporting a learning environment.

Effect of Phosphatidic Acid on Biomembrane: Experimental and Molecular Dynamics Simulations Study.

We consider the impact of phosphatidic acid (namely, 1,2-dioleoyl-sn-glycero-3-phosphate, DOPA) on the properties of a zwitterionic (1,2-dipalmitoyl-sn-glycero-3-phosphocholine, DPPC) bilayer used as a model system for protein-free cell membranes. For this purpose, experimental measurements were performed using differential scanning calorimetry and the Langmuir monolayer technique at physiological pH. Moreover, atomistic-scale molecular dynamics (MD) simulations were performed to gain information on the mixed bilayer's molecular organization. The results of the monolayer studies clearly showed that the DPPC/DOPA mixtures are nonideal and the interactions between lipid species change from attractive, at low contents of DOPA, to repulsive, at higher contents of that component. In accordance with these results, the MD simulations demonstrated that both monoanionic and dianionic forms of DOPA have an ordering and condensing effect on the mixed bilayer at low concentrations. For the DOPA monoanions, this is the result of both (i) strong electrostatic interactions between the negatively charged oxygen of DOPA and the positively charged choline groups of DPPC and (ii) conformational changes of the lipid acyl chains, leading to their tight packing according to the so-called "umbrella model", in which large headgroups of DPPC shield the hydrophobic part of DOPA (the conical shape lipid) from contact with water. In the case of the DOPA dianions, cation-mediated clustering was observed. Our results provide a detailed molecular-level description of the lipid organization inside the mixed zwitterionic/PA membranes, which is fully supported by the experimental data.

Structure of Cholesterol in Lipid Rafts.

Rafts, or functional domains, are transient nano-or mesoscopic structures in the plasma membrane and are thought to be essential for many cellular processes such as signal transduction, adhesion, trafficking, and lipid or protein sorting. Observations of these membrane heterogeneities have proven challenging, as they are thought to be both small and short lived. With a combination of coarse-grained molecular dynamics simulations and neutron diffraction using deuterium labeled cholesterol molecules, we observe raftlike structures and determine the ordering of the cholesterol molecules in binary cholesterol-containing lipid membranes. From coarse-grained computer simulations, heterogenous membranes structures were observed and characterized as small, ordered domains. Neutron diffraction was used to study the lateral structure of the cholesterol molecules. We find pairs of strongly bound cholesterol molecules in the liquid-disordered phase, in accordance with the umbrella model. Bragg peaks corresponding to ordering of the cholesterol molecules in the raftlike structures were observed and indexed by two different structures: a monoclinic structure of ordered cholesterol pairs of alternating direction in equilibrium with cholesterol plaques, i.e., triclinic cholesterol bilayers.