Enteromorpha Intestinalis Research Articles

Fabrication and optimization of ultra-long stable microencapsulated chlorophyll using combinations of wall material via response surface methodology

Nowadays, the need to use natural pigments instead of artificial colorants in food has increased due to health risks. Chlorophyll is a natural colorant with antioxidant properties. Chlorophyll is a natural pigment with superior antioxidant properties. However, this pigment is unstable in the different conditions of food processing. To increase the stability of chlorophyll extracted from Ulva intestinalis algae, encapsulation of chlorophyll with maltodextrin (MD) and whey protein isolate (WPI) carriers by two drying methods including spray drying and freeze drying was achieved. The optimum combination of wall and core materials to achieve the highest response including encapsulation efficiency (EE) and chlorophyll content (CC) was obtained by response surface methodology and central composite design. The optimal chlorophyll microcapsule (Chl-M) obtained was chosen for subsequent tests containing solubility, moisture content, and antioxidant properties. The results showed that the highest EE and CC were 90.27 ± 0.21%, 55.36 ± 0.36 μg/mL, and 90.46 ± 0.62%, 85.85 ± 0.43 μg/mL, respectively in SD and FD. The microcapsules produced by the freeze dryer (FD) had higher antioxidant activity (79.1 ± 0.24) than the microcapsules produced by the spray dryer (SD) (67.5 ± 0.16). The highest solubility (95.32%) and the lowest moisture content (3.7 ± 0.05) were related to the SD. Freeze drying method (FDM) had the highest EE (91.2%), CC (89.67 μg/mL), and antioxidant properties (79.1%). It is hoped that the encapsulated chlorophyll can be used as a health-promoting food color additive in the food industry.

Ulva species from the Romanian Black Sea Coast – between green blooms and nature’s contribution to people

Ulva species have a worldwide distribution and remarkable ability to develop under various conditions, being considered an important natural resource that is under-exploited along the Romanian Black Sea coast. These green algae, involved in green tides observed worldwide, have also a remarkable potential as prolific sources of bioactive compounds with potential health benefits and a wide range of applications in food, feed, pharmaceutical, nutraceutical, cosmetic, or biofuel industries. The study aims to analyze the quantitative variability of Ulva spp. during 2019 - 2023, in order to highlight the areas with algal agglomerations. These are also areas where potential algal deposits can develop during summer season. This fresh biomass can be selectively collected and valorized, thus supporting the circular economy. The sampling method was the classic one (square method/20x20 cm) from 14 stations along Pescarie towards Vama Veche, at depths between 0 to 3 m deep. Among all Ulva spp., Ulva rigida and Ulva intestinalis quantitatively distinguished during the study period, being also the main components of algal deposits. The highest mean wet biomasses were recorded in the southern part, as follows: U. rigida – a maximum value of 2200 g/m2 at 2 Mai; U. intestinalis – max. value of 700 g/m2 at Eforie South. Furthermore, a review of species’ main bioactive compounds, emphasizing their potential health benefits, will also be provided.

Heavy Metal Distributions of Macroalgal Species From the Mersin Inner Bay, Türkiye

The bio-accumulation of heavy metals in macroalgae poses significant risks to marine ecosystems and human health. In this study, heavy metal distributions of macroalgae and potential health risk assessment in the Mersin inner Bay were determined from macroalgae (Stypopodium schimperi, Padina pavonica, Laurencia obtusa, Dictyota sp., Sargassum vulgare, Jania rubens, Halopithys sp., Osmundea sp., Halopithys incurve, Ulva intestinalis, Entromorpha flexuosa, Ulva linza) samples collected between February and September 2021. The levels of heavy metals were found as 1.24-5.93 g/kg for aluminum, 0.61-5.26 g/kg for iron, 1.17-7.61 mg/kg for cobalt, 0.03-0.21 mg/kg for cadmium, 4.26-43.10 mg/kg for chromium, 0.28-437.52 mg/kg for cupper, 17.90-139.08 mg/kg for manganese, 2.78-79.25 mg/kg for nickel, 0.75-3.49 mg/kg for lead and 1.71-70.34 mg/kg for zinc, respectively. The potential risk assessment of macroalgal species using the calculated total cancer risk (TCR) suggested high carcinogenic risk from the carcinogenic chromium, copper and nickel (TCR>1.00E-04).

Optimization and prediction of biogas yield from pretreated Ulva Intestinalis Linnaeus applying statistical-based regression approach and machine learning algorithms

A statistical-based regression approach and machine learning (ML) algorithms (response surface methodology (RSM), feed-forward backpropagation artificial neural network (ANN) and multi-layer adaptive neuro-fuzzy inference system (ANFIS)) were explored for the optimization and prediction of biogas resulting from the anaerobic digestion (AD) of pretreated Ulva Intestinalis Linnaeus (UIL). ANFIS model was found to better predict and model the process of biogas production from the AD of pretreated UIL owing to low mean square error (MSE) and RMSE values (0.8841 and 0.9402-US, 0.9628 and 0.9812-O3, 0.1387 and 0.3724-MW and 0.3018 and 1.1410-Fe3O4) and highest values of R2 (0.9998-US, 0.9996-O3, 0.9996-MW and 0.9995-Fe3O4). Optimum conditions for biogas yield as a result of the various pretreatment processes based on the ANFIS model were US-15 power/time, time-40 min and the biogas yield-181.0 mL.gVS−1, O3-15.0 mg/min, time-40.0 min and biogas yield of 164.0 mL.gVS−1, MW-2.3 power/time, time-40.0 min and biogas yield-81.7 mL.gVS−1 and Fe3O4-20.0 mg L−1, time-40.0 min and biogas yield-154 mL.gVS−1. The results obtained show that the ML and statistical-based models were effective in approximating the biogas yield with high precision and low error and could be beneficial for the biogas production scale-up process.

Potential anti-obesity impact of green seaweed; Enteromorpha intestinalis

Potential anti-obesity impact of green seaweed; Enteromorpha intestinalis

Evaluation of Antioxidant Activity of Residue from Bioethanol Production Using Seaweed Biomass.

This study explores the potential of producing bioethanol from seaweed biomass and reusing the residues as antioxidant compounds. Various types of seaweed, including red (Gelidium amansii, Gloiopeltis furcata, Pyropia tenera), brown (Saccharina japonica, Undaria pinnatifida, Ascophyllum nodosum), and green species (Ulva intestinalis, Ulva prolifera, Codium fragile), were pretreated with dilute acid and enzymes and subsequently processed to produce bioethanol with Saccharomyces cerevisiae BY4741. Ethanol production followed the utilization of sugars, resulting in the highest yields from red algae > brown algae > green algae due to their high carbohydrate content. The residual biomass was extracted with water, ethanol, or methanol to evaluate its antioxidant activity. Among the nine seaweeds, the A. nodosum bioethanol residue extract (BRE) showed the highest antioxidant activity regarding the 2,2-diphenyl-1-picrylhydrazyl (DPPH) activity, ferric reducing antioxidant power (FRAP), and reactive oxygen species (ROS) inhibition of H2O2-treated RAW 264.7 cells. These by-products can be valorized, contributing to a more sustainable and economically viable biorefinery process. This dual approach not only enhances the utilization of marine resources but also supports the development of high-value bioproducts.

Effect of distinct drying approach on bioactive compounds and nutritional profiling of Ulva intestinalis and Padina tetrastromatica from the Bay of Bengal, Bangladesh

Marine algae have been visualized as fostering a prevalent actuation of bioactive substances that have noteworthy therapeutic effects. The current investigations deployed a trio of separate drying methods, i.e., oven drying, freeze drying, and sun drying, to quantify the total phenolic content (TPC), total flavonoid content (TFC), and dietary vitality of Ulva intestinalis and Padina tetrastromatica. By mining and scrutinizing it to the quercetin and gallic acid standards, TPC and TFC were derived. Among three drying techniques, freeze drying asserted higher TPC (12.59 ± 1.07 in U. intestinalis and 68.74 ± 1.95 mg of GA/g in P. tetrastromatica) and TFC (7.92 ± 0.97 in U. intestinalis and 58.15 ± 2.05 mg of quercetin/g in P. tetrastromatica) values. Ulva intestinalis and P. tetrastromatica also contain a high amount of protein (18.34% and 8.7%, respectively). The findings demonstrated that green seaweed possessed higher levels of potassium (2.20%) and molybdenum (2.41%) and brown seaweed showed higher amounts of potassium (2.41%) and calcium (1.11%). Where rapid drying methods are recommended to enhance constituent levels, freeze-drying could be an attractive alternative to oven and sun drying, as it addresses the drawbacks associated with prolonged drying periods.

Dietary administration of green macroalgae (Ulva intestinalis) on growth performance, serum immune parameters, and gene expression in Common carp (Cyprinus carpio)

Abstract The purpose of this experiment was to assess the effects of the green macroalgae (Ulva intestinalis) on growth performance, immune parameters of serum and mucus, liver enzymes and the expression of genes related to growth (GH and IGF-1), and immunity (IL1-β and Lyz) in common carp (Cyprinus carpio). The test was carried out in eight weeks with the treatments supplemented with 0 (control), 0.25%, 0.5%, and 1% macroalgae and three replications for each group. At the end of the course, growth indices, serum and mucus total protein, total immunoglobulin, lysozyme, and liver enzymes including alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase were evaluated. Additionally, the expression of growth (GH and IGF-1) and Immunity (IL1-β and Lyz) genes was evaluated using real-time PCR in the brain, liver, and intestine. Based on the results, a significant difference was observed in the levels of total protein, total immunoglobulin, and mucus lysozyme in the fish that received 1% U. intestinalis compared to the control group (P<0.05). In addition, the amount of total protein, total immunoglobulin, and lysozyme in the control group and the other treatments represented a significant difference (P<0.05). Although the initial weight did not show a significant difference among the treatments (P>0.05), the final growth parameters significantly varied in different treatments, with the highest final weight observed in fish fed with 0.25% algae (P<0.05). The results of the expression of immune genes lysozyme (Lyz) and interleukin-1 (IL-1) indicated a significant difference with the control group (P<0.05), representing an increasing pattern with the rising percentage of macroalgae (P<0.05). The supplement was also found effective in promoting the expression of growth hormone (GH) and insulin-like growth factor (IGF-1) compared to the control fish (P<0.05), revealing an increasing pattern for the IGF-1 gene with the elevated level of the supplement. In general, according to the results of the present study, U.intestinalis can be recommended in carp aquaculture, most preferably at the level of 1% of daily feed, as it could significantly enhance the growth and innate immunity of fish.

Phenolic compound derived from Enteromorpha intestinalis and their bioactivity against bacterial pathogens

The purpose of the study was to assess a phenolic compound that was extracted from the brown seaweed Enteromorpha intestinalis (Linn.), Nees (1820) for its antibacterial activities on different bacterial pathogens of fish illnesses. Hexane and methanol were utilized to extract bioactive compounds from the seaweed. Phytochemical analysis of the methnaolic extracts have revealed the presence of carbohydrates and glycosides, saponins, alkaloids, amino acids, flavonoids, fixed oil and fat, phenol compounds, tannins, and steroids. The bacterial pathogens of fish, including Providencia vermicola, Aeromonas hydrophila, Vibrio harveyi, and Aeromonas caviae, were targeted for antibacterial assessment. Among them, the methanolic crude extract and fraction-1 of E. intestinalis have shown maximum activity against A. hydrophila (22 ± 0.13 and 33 ± 0.56 µg/mL). Column chromatography was employed to isolate effective seaweed fraction, while FT-IR and HPLC were utilized for characterization. The phytochemical analysis confirmed the presence of various compounds in both extracts. The crude methanolic extract of E. intestinalis have demonstrated the maximum efficacy beside the tested bacterial pathogens. The FT-IR analysis revealed functional groups associated with polymeric structures in the seaweed extracts, including alcohols, phenols, aromatics, alkyl halides, aliphatic amines, alkynes, and alkenes. Overall, the methanolic extract of E. intestinalis exhibited significant antibacterial activity as a result of the phenolic chemicals that are present. The study provides valuable baseline data, emphasizing the need for further research towards developing antibacterial drug formulations.

Sustainable and eco-friendly production of biodiesel from Chlorella vulgaris supplemented with biogenic calcium oxide nanoparticles derived from Ulva intestinalis and eggshell as precursors

Biodiesel derived from microalgae has the potential to be a sustainable fuel. Eggshell waste is rich in vital compounds, but still creates pollution problems, due to being discarded in the landfills without any treatment. This study aims to convert eggshells to CaCl2 and use CaCl2 derived from eggshells to biogenic CaO-NPs. Tested the impact of CaCl2, CaCl2 derived from eggshells, and biogenic CaO-NPs, as supplementation to the BG11 media on the growth and lipids yield of Chlorella vulgaris green alga. This study used Ulva intestinalis green alga to synthesize (Ui-CaO-NPs). The characterizations of Ui-Ca-ONPs were analyzed by EDS, XRD, zeta potential, FT-IR, and TEM. Chlorella vulgaris cultivated in modified BG11 media that was supplemented by different concentrations of CaCl2, CaCl2 derived from eggshell, and Ui-CaO-NPs as eggshell precursors (0.009, 0.018, and 0.036 g/L). The low concentrations of CaCl2 as eggshell precursors possessed high optical density, dry weight, and lipids contents of C. vulgaris. The best Chlorophyll a and b contents were obtained with Ui-CaO-NPs (0.018 g/L). The highest carotenoid content was obtained with alga grown in a medium containing 0.036 g/L CaCL2 the GC.Ms. analysis possessed the high-quality lipids profile obtained with C. vulgaris cultivated in media containing 0.018 g/L Ui-CaO-NPs. Eggshells can be used as sources of CaCl2 and CaO-NPs for different applications as eco-friendly and economical materials.

Nano iron loaded algal biomass: For better yield, amino acid and iron content in rice – A ‘nano-phycofertilizer’

The iron nano particles (INPs) loaded algal biomass can stimulate plant growth, leading to improved crop yields and quality. In this article, we have explored the efficacy of INPs-loaded biomass of Ulva intestinalis as ‘Nano-Phyco-Fertilizer (NPF)’ for rice cultivation. In situ INPs synthesis by Ulva biomass was spontaneous, turning the green thallus to brown due to the deposition of a large amount of intracellular INP within 24 h in dark conditions. The synthesized INPs was characterized as spindle-shaped with ferromagnetic properties by TEM analysis. The INPs-loaded algal biomass was dried, dusted, and applied to soil as NPF. Additionally, algal biomass without iron and free INPs were also tested separately and compared to NPF. The synergistic combination of algae and INPs in fertilizer formulations is a novel work and it offers several advantages over conventional fertilizers. As a result, after the application of NPF, the heights and grain yield of rice plants increased by 40 % and 2.5 times respectively. Moreover, the essential amino acid and iron content in rice grains showed a significant increase by 20–40 % and 45 % respectively upon NPF application, as compared to that of rice crops with conventional agricultural practice. The integration of algal and INP-based fertilizers represents a sustainable and innovative approach to meet the growing demands of global agriculture. As a result, it can be concluded that NPF shows significant enhancement in the growth and productivity of rice plant cultivation and holds immense promise in ensuring food security and mitigating the environmental impacts associated with conventional fertilization malpractices.

Quality Characteristics of Nutritional Bar with Varying Concentrations of Enteromorpha intenstinalis Powder

This study analyzed the quality characteristics of nutritional bars with varying concentrations (0, 0.5, 1.5, 2.5%) of Enteromorpha intenstinalis powder added. To achieve this, color analysis, an assessment of DPPH radical scavenging activities, and sensory evaluations were performed. The results revealed that as the amount of Enteromorpha intestinalis powder (EIP) increased, pH and lightness values decreased. DPPH radical scavenging activities of 75.26%, 56.11%, 71.59%, and 62.49% were exhibited for the 0%, 0.5%, 1.5%, and 2.5% concentrations, respectively at 50 mg/mL. Significant differences were observed between the samples with respect to the color, appearance, texture, taste, and overall preference for the nutritional bars. Overall preference was highest for the nutritional bar with 1.5% Enteromorpha intenstinalis powder added. Therefore, adding 1.5% of Enteromorpha intenstinalis powder to a nutritional bar is most suitable for enhancing preference and quality characteristics.

Nutritional composition and phenolic contents of Gracilariopsis longissima, Padina tetrastromatica and Ulva intestinalis from the Bay of Bengal, Bangladesh coast

Seaweeds have become the focus of experts in recent years due to their biological characteristics and the variety of uses they have for both humans and animals. Biochemical composition, amino acids, fatty acids, and phenolic components were analyzed to explore the nutritive value of Gracilariopsis longissima, Padina tetrastromatica, and Ulva intestinalis from the Bay of Bengal, Bangladesh coast. Proximate composition and mineral content were determined according to the AOAC method. The high-performance liquid chromatography amino acid analysis system was used for the amino acid analysis and the fatty acid profile of the extracted oils was assessed as their methyl esters. The Folin–Ciocalteu technique was used to estimate the phenolic content and the aluminum chloride colorimetric technique was used to calculate the total flavonoid content. The three different species of seaweed had significantly different proximate compositions (P < 0.05), with G. longissima having the highest protein content. Except for sulfur, the mineral contents were likewise considerably higher (P < 0.05) in G. longissima. Although the amounts of the essential amino acids were greater than 50 % of the total amino acids in the three studied seaweed species, the total amino acid composition of these three species differed significantly (P < 0.05). The findings indicated that lipid levels were low in all the assessed species, but unsaturated fatty acid levels were high, with G. longissima exhibiting the highest amounts. The results showed that, compared to the other species, G. longissima had a substantially higher (P < 0.05) level of total phenolic and flavonoid content. The three studied seaweed appear to be excellent for nutrition based on their overall nutritional profiles. However, due to high protein, unsaturated fatty acid, essential amino acid, and total phenolic and flavonoid content, G. longissima is the most promising seaweed that will be helpful for pharmaceutical and multifunctional food applications.

Efficacy and effects of three desiccation intervals on biofouling and Eastern oysters (Crassostrea virginica) at three commercial oyster farms in the Chesapeake Bay, MD

Oyster aquaculture is a growing industry both globally and nationally. Productive coastal waters support high-density containerized culture of oysters, but also sustain many other macrofaunal species which can settle on oysters and cage materials (biofouling), causing problems for oyster farms. Desiccation, or periodic air drying, of farm infrastructure and oysters has been used to control colonization and growth of biofouling organisms on oyster farms worldwide, but the minimum necessary interval of desiccation has yet to be thoroughly assessed. Given the known stresses to oysters associated with periods of emersion, an understanding of the effects of different weekly desiccation intervals is needed. This study investigated the use of desiccation in controlling biofouling and the associated impacts to oyster growth and mortality at three oyster farms within Maryland's portion of the Chesapeake Bay, including one low salinity site in an upper Chesapeake Bay tributary and two sites in the middle portion of the Chesapeake Bay. Three desiccation treatments were applied at each site: control (no desiccation), 8 consecutive hours of desiccation per week, and 24 consecutive hours of desiccation per week. Oysters were deployed between July–December 2018 and the percent valve coverage by individual biofouling species was monitored, along with worm abundance, oyster growth, and oyster mortality. Total biofouling was significantly greater on non-desiccated oysters but minimal differences in biofouling coverage were observed among oysters desiccated for 8 or 24 h weekly. Seven biofouling species were observed among the sites, and all except for a single macroalgal species (Ulva intestinalis) were controlled by the 8- and 24-h desiccation treatments. No clear treatment-derived differences in oyster growth or mortality were observed. Overall, results suggest desiccating weekly for either 8 or 24 consecutive hours may be a suitable biofouling management strategy for gross biofouling reduction on oyster farms.

Potential use of Ulva intestinalis-derived biochar adsorbing phosphate ions in the cultivation of winter wheat Tristicum aestivum

In this work, the properties of biochar produced from green macroalga Ulva intestinalis by pyrolysis were studied at temperatures of 300, 500, and 700 °C. This biochar was characterized in terms of multielemental composition, BET surface area, total pore volume, and biosorption properties toward phosphate ions. Biochar produced at 700 °C–25 m2/g had the highest surface area. The kinetics and isotherms of sorption processes of phosphate ions as sorbate by these sorbents were investigated. Modified biochar was able to remove 84.3% of phosphate ions from wastewater, whereas non-modified biochar—only 40.6%. Hence, biochar enriched with phosphate ions can serve as a valuable soil amendment. Pot experiments performed on winter wheat (Triticum aestivum) with a 3% addition of dry Ulva intestinalis, pristine biochar, and Mg-modified biochar enriched with phosphate ions showed that these amendments stimulated plant growth (length and fresh weight of plants) as well as enlarging the chlorophyll content in leaves. Our results indicate that the production of biochar (pristine and Mg-impregnated) is a sustainable option to valorize the biomass of seaweeds, and to recycle phosphorus from wastewater.Graphical

Biosynthesis of Silver Nanoparticles Using Ethanolic Extract of the Marine Alga Enteromorpha intestinalis and Evaluation of their Antibacterial Activity

Biosynthesis of Silver Nanoparticles Using Ethanolic Extract of the Marine Alga Enteromorpha intestinalis and Evaluation of their Antibacterial Activity

Dual-function macroalgae biochar: Catalyst for hydrogen production and electrocatalyst

In the current study, Enteromorpha intestinalis, a green macroalgae, has been utilized as a substrate to synthesise a new environmentally friendly and cheap dual-functional (catalyst and electrocatalyst) material. The catalyst was used for efficient hydrogen production from alcoholises sodium borohydride and as an anode catalyst for direct methanol fuel cell applications. At the catalyst synthesis stage, orthogonal arrays of Taguchi is used to find the optimum levels of independent variables for the superior catalyst performance that bears the modest kinetics. The experiments performed in accordance with the L16(45) type orthogonal array. The samples treated at relatively moderate acid, impregnation temperature, impregnation time, burning temperature and burning time showed higher catalytic activities with Exp(5) presenting the optimal catalytic activity followed by Exp(1), Exp(15), Exp(8) and Exp(12), respectively. The experimental levels of coded variables (acid molarity, impregnation temperature and time, burning temperature and time) for Exp(5) were 3 M, 50 °C, 24 h, 500 °C and 2 h., respectively. These finding suggest that providing maximum levels of each of independent variable will not provide high catalytic activity. Taking into account the binary and ternary interactions is an efficient way to determine optimal level of each parameter with regards to maximum hydrogen production rate. The morphological and structural characterization of the optimal catalyst was finally carried out with SEM- EDS, XRD and FT-IR. CV and EIS measurements were taken to determine the electrocatalytic activity of final biochar. Experimental studies revealed that the utilization of E. intestinalis-based electrocatalyst as an anode catalyst for direct fuel cell applications is promising. By employing advanced Taguchi-based experimentation, this research establishes the optimum levels of independent variables, enhancing the catalyst's performance, and highlighting a novel approach to modest kinetics improvement.

Effect of Seaweed Liquid Fertilizer on Sorghum bicolor and Pennisetum glaucum

Millets are small-seeded grasses belonging to the family Poaceae that help in human and animal nourishment. They are mostly grown by using chemical fertilizers which are depleting soil fertility. Organic fertilizers are eco-friendly and help to enhance the growth and development of plants thereby maintaining soil health. Seaweed liquid fertilizer (SLF) are organic fertilizers that can be used to control the side effects caused by harmful chemical fertilizers. The present study was carried out to check the effects of seaweed liquid fertilizer on the growth and development of millets. Different concentrations of seaweed liquid fertilizer (0.25%, 0.50%, and 0.75%) were prepared from Sargassum cinerum, Ulva intestinalis, and Padina tetrastromatica, which were tested on Sorghum bicolor and Pennisetum glaucum. Growth parameters like root length, shoot height, flowering-fruiting, and biochemical analysis like proteins were analyzed by using seaweed liquid fertilizer and chemical fertilizer. Plants treated with 0.50% concentration of seaweed liquid fertilizer showed higher protein content. Total chlorophyll was found to be higher in treated plants than in control plants. Flowering and fruiting were observed earlier in seaweed liquid fertilizer treated plants. This concludes that seaweed extracts can be used as a promising alternative to chemical fertilizers, which plays a significant role in the holistic growth enhancement of plants.

Antibacterial activity of flavonoid extracts from Enteromorpha intestinalis and Caulerpa prolifera against multidrug-resistant foodborne bacterial isolates.

Food poisoning caused by bacterial agents is a worldwide problem, usually accompanied by unpleasant symptoms and may be severe leading to death. Natural compounds from marine algae namely flavonoids may play a role in the remedy of this condition. This research aims to assess the potency of flavonoids extracted from Enteromorpha intestinalis and Caulerpa prolifera as antibacterial agents. Enteromorpha intestinalis was collected from Western Libyan Coast and C. prolifera was collected from Farwa Island. The antimicrobial activity and determination of minimum inhibitory concentration of algal flavonoid-containing extracts was performed in vitro against some positive and negative Gram bacteria. Crude extract containing flavonoids from E. intestinalis was more effective than C. prolifera extract against Staphylococcus aureus with antimicrobial essay (25-28 + 1 and 14.5-37.5 + 0.5-1.5), MIC (50 and 50-250 µg/ml), MBC (75 and 75-250 µg/ml). In Bacillus cereus, the antimicrobial assay (19-24.5 + 0.5-1.5: 24 + 1), MIC (50-250 + 100 µg/ml), and MBC (250 and 125 µg/ml). On the other hand, flavonoids containing extract from C. prolifera were more effective than E. intestinalis against Enterohemorrhagic Escherichia coli O157 EHEC O157 (25-28 + 1: 14-18.5 + 0.5-1.5), MIC (100-250:100-500 µg/ml), and MBC (150-250 and 250-500 µg/ml). Salmonella enterica qualitatively combat by flavonoid from E. intestinalis (13.5-14 + 0.5-1: 10.5-13.5 + 0.5-1.5), MIC (100-250: 250 µg/ml), and MBC (100-250: 250 µg/ml). Flavonoids from C. prolifera (4 strains: 2 strains) were effective against S. enterica. Crude flavonoids from both algae were not effective against Bacillus pumilus. Data from this study could conclude that flavonoid extracts from E. intestinalis and C. prolifera could be used against foodborne bacterial agents.

Marine macroalgae (Enteromorpha intestinalis) for improving the growth performance, meat quality traits, and serum biochemical parameters in broilers.

The purpose of the current study was to examine the effectiveness of green macroalgae as a novel, natural feed additive for broilers that have a greater concentration of active ingredients. Four experimental groups of 180-day-old male broiler chicks (Cobb-500) were randomly assigned, with three replicates in each group: the control group [T0, maintained only with basal diet] and three treatment groups supplemented with macroalgae for 35 days along with basal diet [T1 = 0.05% (w/w); T2 = 0.1% (w/w); T3 = 0.2% (w/w) macroalgae]. Live weight, carcass weight, and organs' weight were noted at the conclusion of the experiment. The meat quality was examined using the muscles of the thighs and breasts, and blood serum was obtained for biochemical assessment. The results revealed that dietary supplementation of green macroalgae (0.1%) in broiler rations significantly (p < 0.05) improved the growth performance compared to other treated groups and controls. With increasing weight, it enhanced meat quality traits assessed by increased water holding capacity, ultimate pH, redness and yellowness, and decreased lightness of muscles in the thighs and breasts. Both the levels of serum cholesterol and abdominal fat decreased and showed no unwholesome effects on liver and kidney functions. For the production of safe and high-quality poultry meat, marine green macroalgae (Enteromorpha intestinalis) could be used as a potential feed additive. It enhanced the growth rate in broilers and improved meat quality and serum biochemical parameters for supplying healthy meat in the human food chain.