Ultraviolet Germicidal Irradiation System Research Articles

A data mining-based framework to define optimal operation strategies for in-duct ultraviolet germicidal irradiation systems for different climatic conditions

This paper presents a novel data mining framework for optimizing the operation of in-duct Ultraviolet germicidal irradiation (UVGI) systems employed for air disinfection within air handling units. The performance of the UVGI system is influenced by the operating conditions of air handling units. As a result, under favourable operating conditions, the system produces excessive Ultraviolet C energy beyond the required disinfection levels, causing energy waste and accelerating the degradation of heating, ventilation, and air conditioning (HVAC) components. The framework aims to improve energy efficiency and mitigate material degradation within HVAC components by systematically adjusting UVGI system output based on changing operating conditions of air handling units throughout the year using advanced data mining techniques. The methodology involves several steps: Firstly, data pre-processing is performed to improve data quality. Then, the K-means clustering algorithm is applied to discover patterns in operational data. Subsequently, an effective performance index and baseline are defined to identify energy-saving opportunities within each cluster. Finally, the optimal operation strategy is defined based on the determined baseline. The framework is extended to five distinct climate zones to derive region-specific operation strategies. Results from a case study in a small office building in Montreal and an extension to five distinct climate zones in Canada demonstrate energy savings from 8% to 37%. This study offers a promising approach to optimize UVGI system operation, with potential applications in various energy systems in buildings requiring adaptive operational conditions for optimal performance.

Effects of environmental factors, measurement and modeling for germicidal efficiency of in-duct ultraviolet germicidal irradiation system

In-duct ultraviolet germicidal irradiation (UVGI) systems effectively decontaminate airborne microorganisms that are present in duct air. In this study, the main objectives were to determine the effects of temperature, relative humidity (RH), air velocity, and measurement on the germicidal efficiency of in-duct UVGI and to analyze three germicidal kinetic models. Staphylococcus albus and Escherichia coli were selected as the test bacteria. The maximum UV disinfection efficacy was observed in the temperature range of 26–28 °C and RH range of 30–40 %. The germicidal efficiency of the in-duct UVGI was negatively corelated with air velocity. When the UV lamps were arranged either parallel or vertical to the airflow, there were no obvious differences in-duct UVGI germicidal efficiency. In addition, three mathematical prediction models (C-W model, C-S model, Hom model) were chosen to predict the efficacy of in-duct UVGI in specific applications. The C-S model was fitted to predict the efficacy of E. coli, and the Hom model was fitted to predict that of Staphylococcus albus.

Rib-induced eddies increase inactivation efficiency and air handling capacity of in-duct ultraviolet germicidal irradiation systems with low energy cost

Attaching ribs on duct walls is an effective way to improve the inactivation efficiency of in-duct UVGI systems (ID-UVs), and this study explored the optimal rib design. The Critical Survival Fraction Probability (CSFP) and Maximal Bearable UV Dose (MBUD) methods were employed as indicators. Results showed that ID-UVs with ribs could be optimized by balancing the positive effect of rib-induced eddies and the negative effect of rib-induced high airflow velocity. The optimal rib spacing was equal to the duct height (namely, 1.0 H), the optimal shape was two rows of trapezoidal ribs aligned the same direction. The best inactivation performance was achieved by attaching 0.15 H ribs on all walls alone downstream of lamps. Compared to the condition without ribs, the optimal ID-UVs increased inactivation efficiencies by 31.4 % (CSFP, 10.5 times greater than that achieved by optimizing lamp array) and 68.6 % (MBUD) for the highly UV-susceptible MS2 Bacteriophage, and, up to 146.4 % (MBUD) for the low UV-susceptible MS2 Bacteriophage. The optimal ID-UVs additionally allowed a 10–20 % increase in air handling capacity and an 85.3 % reduction in annual energy cost, when compared to conventional conditions with the same air-cleaning level. These findings suggest promising prospects for implementing the ID-UVs with ribs.

UVC-Mirror for effective pathogens inactivation in air ducts

Improving the air quality of indoor environments (IAQ) is of utmost importance to safeguard public health as people spend about 80–90% of their time indoor. Efficient Ultraviolet germicidal irradiation (UVGI) system represents a strategic and sustainable solution to protect from recurrent and new airborne pathogens. Here, we present a new approach to design highly efficient UVGI systems, which can be installed in existing Air Treatment Units (ATU) plants with minimal effort. The increased efficiency relies on the concept of an optical cavity, thanks to its shape and source position. The internal volume consists of a highly reflective cavity illuminated with UV-C lamps. Optical simulations permitted the variation of the parameters to maximize the internal irradiance and, thus, the performance. The sanitation efficacy of the system was assessed on a full-scale pilot system. Tests were carried out under normal operating conditions against various microorganisms showed an inactivation rate of > 99%. The benefits of such systems are triple and encompass economic, environmental, and societal aspects. Since the system requires little energy to operate, its application for air disinfection may yield significant energy savings and ensure a balance between energy sustainability and good IAQ.

Ultraviolet germicidal irradiation: A prediction model to estimate UV-C-induced infectivity loss in single-strand RNA viruses

Ultraviolet germicidal irradiation (UVGI) disinfection technology is effective in inactivating microorganisms. However, its performance can vary against different microorganisms due to their diverse structural and genomic features. Thus, rapid predictions of UV (254 nm) inactivation kinetics are essential, particularly for highly infectious emerging pathogens, such as SARS-CoV-2, during the extemporary COVID-19 pandemic. In this study, aiming at single-strand RNA (ssRNA) viruses, an improved genomic model was introduced to predict the UV inactivation kinetics of viral genomes using genome sequence data. First, the overall virus infectivity loss in an aqueous matrix was estimated as the sum of damage to both the entire genome and the protein capsid. Then, the “UV rate constant ratio of aerosol and liquid” was used to convert the UV rate constant for viruses in a liquid-based matrix to an airborne state. The prediction model underwent both quantitative and qualitative validation using experimental data from this study and the literature. Finally, with the goal of mitigating potential airborne transmission of ssRNA viruses in indoor environments, this paper summarizes existing in-duct UVGI system designs and evaluates their germicidal performance. The prediction model may serve as a preliminary tool to assess the effectiveness of a UVGI system for emerging or unculturable viruses or to estimate the required UV dose when designing such a system.

Reducing airborne transmission of SARS-CoV-2 by an upper-room ultraviolet germicidal irradiation system in a hospital isolation environment

Upper-room ultraviolet germicidal irradiation (UVGI) technology can potentially inhibit the transmission of airborne disease pathogens. There is a lack of quantitative evaluation of the performance of the upper-room UVGI for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) airborne transmission under the combined effects of ventilation and UV irradiation. Therefore, this study aimed to explore the performance of the upper-room UVGI system for reducing SARS-CoV-2 virus transmission in a hospital isolation environment. Computational fluid dynamics and virological data on SARS-CoV-2 were integrated to obtain virus aerosol exposure in the hospital isolation environment containing buffer rooms, wards and bathrooms. The UV inactivation model was applied to investigate the effects of ventilation rate, irradiation flux and irradiation height on the upper-room UVGI performance. The results showed that increasing ventilation rate from 8 to 16 air changes per hour (ACH) without UVGI obtained 54.32% and 45.63% virus reduction in the wards and bathrooms, respectively. However, the upper-room UVGI could achieve 90.43% and 99.09% virus disinfection, respectively, with the ventilation rate of 8 ACH and the irradiation flux of 10 μW cm−2. Higher percentage of virus could be inactivated by the upper-room UVGI at a lower ventilation rate; the rate of improvement of UVGI elimination effect slowed down with the increase of irradiation flux. Increase irradiation height at lower ventilation rate was more effective in improving the UVGI performance than the increase in irradiation flux at smaller irradiation height. These results could provide theoretical support for the practical application of UVGI in hospital isolation environments.

CFD modeling of room airflow effects on inactivation of aerosol SARS-CoV-2 by an upper-room ultraviolet germicidal irradiation (UVGI) system

Ultraviolet germicidal irradiation (UVGI) systems inactivate microorganisms indoors. Upper-room UVGI systems use wall- or ceiling-mounted fixtures to create an air disinfection zone above the occupied zone. The performance of upper-room UVGI systems varies with indoor airflow patterns induced by mechanical ventilation and thermal plumes from indoor heat sources. Little information is available on the effects of ventilation strategies on upper-room UVGI system performance for the control of viral aerosols in occupied spaces. This study simulated the effects of ventilation system characteristics in an office space on the ability of an upper-room UVGI system to inactivate viral aerosols with UV-C susceptibility representative of coronaviruses. UVGI reduced viral aerosol concentration by two orders of magnitude relative to the concentration without UVGI. Air change rates and air distribution strategy (mixing vs. displacement) had notable effects on the effectiveness of the UVGI system. For mixing ventilation, as the recirculation airflow rate increased from 0 to 5.3 h−1 for a room volume of 108 m3 with a fixed outdoor air change rate of 0.7 h−1, UVGI inactivation increased by 96.7%. Mixing ventilation with 100% outdoor air of 0.7 h−1 yielded airborne virus inactivation that was double that of displacement ventilation, due to enhanced air mixing.

Effect of ventilation strategy on performance of upper-room ultraviolet germicidal irradiation (UVGI) system in a learning environment

Upper-room ultraviolet germicidal irradiation (UVGI) system is recently in the limelight as a potentially effective method to mitigate the risk of airborne virus infection in indoor environments. However, few studies quantitatively evaluated the relationship between ventilation effectiveness and virus disinfection performance of a UVGI system. The objective of this study is to investigate the effects of ventilation strategy on detailed airflow distributions and UVGI disinfection performance in an occupied classroom. Three-dimensional computational fluid dynamics (CFD) simulations were performed for representative cooling, heating, and ventilation scenarios. The results show that when the ventilation rate is 1.1 h−1 (the minimum ventilation rate based on ASHRAE 62.1), enhancing indoor air circulation with the mixing fan notably improves the UVGI disinfection performance, especially for cooling with displacement ventilation and all-air-heating conditions. However, increasing indoor air mixing yields negligible effect on the disinfection performance for forced-convection cooling condition. The results also reveal that regardless of indoor thermal condition, disinfection effectiveness of a UVGI system increases as ventilation effectiveness is close to unity. Moreover, when the room average air speed is >0.1 m/s, upper-room UVGI system could yield about 90% disinfection effect for the aerosol size of 1 μm–10 μm. The findings of this study imply that upper-room UVGI systems in indoor environments (i.e., classrooms, hospitals) should be designed considering ventilation strategy and occupancy conditions, especially for occupied buildings with insufficient air mixing throughout the space.

Development and experimental validation of an improved mathematical irradiance model for in-duct ultraviolet germicidal irradiation (UVGI) applications

The application of ultraviolet germicidal irradiation (UVGI) technology inside the heating, ventilation, and air-conditioning (HVAC) air ducts to purify circulating air and improve indoor air quality has attracted extensive interest during the COVID-19 pandemic. In this study, a new view-factor-based mathematical model was developed to calculate the irradiation distribution for a typical twin-tube UV lamp placed at the center of a square duct, in which the contributions from direct emissive irradiance, specular reflection irradiance, and diffuse reflection irradiance were quantified. Furthermore, the “projection area” method was introduced to mathematically estimate the shadowing effects between the two lamps by considering multiple-lamp scenarios in real in-duct UVGI system designs. Subsequently, a computational fluid dynamics (CFD) simulation was employed to compute the average received UV dose and disinfection efficiency of the system. The mathematical model combined with the CFD simulation was validated using the experimental data. It is concluded that by increasing the UV lamps, UV lamp power, and using more reflective duct wall materials, the in-duct UVGI disinfection performance can be improved. For the multiple-lamp arrangements, placing lamps perpendicular to the airflow in the same row results in a more uniform irradiance distribution and higher overall irradiation than placing them in different rows along the duct, thus increasing the disinfection efficiency. In addition, the duct wall with highly diffusive reflection provides a more uniform irradiance distribution and overall higher average radiation, thus providing better disinfection performance for an in-duct UVGI reactor.

Performance of upper-room ultraviolet germicidal irradiation (UVGI) system in learning environments: Effects of ventilation rate, UV fluence rate, and UV radiating volume

• We study performance of upper-room ultraviolet germicidal irradiation (UVGI) systems. • Performance of upper-room UVGI system in a classroom varies with ventilation rate. • Non-uniform airflow pattern meaningfully influences the UVGI system performance. • UV radiating volume is more crucial for virus disinfection than UV fluence rate. • Doubling UV fluence rate from 25 to 50 μW∙cm-2 yields only moderate benefit. Previous studies show that upper-room ultraviolet germicidal irradiation (UVGI) systems can help contain infectious airborne viruses indoors. However, there has been a lack of research on the performance of an upper-room UVGI system in learning environments such as a school classroom. Since classrooms are more vulnerable to airborne transmission of diseases due to high occupancy for long hours, airborne infection characteristics are different from other occupied indoor environments (e.g., offices and residences). The objective of this study is to investigate UVGI system performance in a classroom considering detailed effects of ventilation rate, UV fluence rate, and UV radiating volume. Two analytical models, a one-zone and a two-zone material balance model, along with computational fluid dynamics (CFD) simulations, were employed to analyze viral aerosol concentrations under a representative range of classroom operating conditions. The CFD results show that increasing ventilation rate from 1.1 h –1 to 5 h –1 yields about 85% of airborne disinfection while doubling UV radiating volume results in a 60% disinfection. However, increasing UV fluence rate from 25 μW∙cm –2 to 50 μW∙cm –2 yields a moderate additional disinfection of 18%. Overall, the study results reveal that operating a UVGI system in an occupied classroom can markedly disinfect airborne viruses up to 96%, which is as effective as increasing ventilation rate more than five times. Furthermore, the results suggest that the one-zone and two-zone analytical models used in several previous studies could result in notably meaningful errors in analyzing viral aerosol concentrations, especially in occupied rooms with a highly non-uniform airflow distribution.

The impact of heating, ventilation, and air conditioning design features on the transmission of viruses, including the 2019 novel coronavirus: A systematic review of ultraviolet radiation.

Respiratory viruses are capable of transmitting via an aerosol route. Emerging evidence suggests that SARS-CoV-2 which causes COVID-19 can be spread through airborne transmission, particularly in indoor environments with poor ventilation. Heating, ventilation, and air conditioning (HVAC) systems can play a role in mitigating airborne virus transmission. Ultraviolet germicidal irradiation (UVGI), a feature that can be incorporated into HVAC systems, can be used to impede the ability of viruses to replicate and infect a host. We conducted a systematic review of the scientific literature examining the effectiveness of HVAC design features in reducing virus transmission—here we report results for ultraviolet (UV) radiation. We followed international standards for conducting systematic reviews and developed an a priori protocol. We conducted a comprehensive search to January 2021 of published and grey literature using Ovid MEDLINE, Compendex, and Web of Science Core. Two reviewers were involved in study selection, data extraction, and risk of bias assessments. We presented study characteristics and results in evidence tables, and synthesized results across studies narratively. We identified 32 relevant studies published between 1936 and 2020. Research demonstrates that: viruses and bacteriophages are inactivated by UV radiation; increasing UV dose is associated with decreasing survival fraction of viruses and bacteriophages; increasing relative humidity is associated with decreasing susceptibility to UV radiation; UV dose and corresponding survival fraction are affected by airflow pattern, air changes per hour, and UV device location; and UV radiation is associated with decreased transmission in both animal and human studies. While UV radiation has been shown to be effective in inactivating viruses and reducing disease transmission, practical implementation of UVGI in HVAC systems needs to consider airflow patterns, air changes per hour, and UV device location. The majority of the scientific literature is comprised of experimental, laboratory-based studies. Further, a variety of viruses have been examined; however, there are few studies of coronaviruses and none to date of SARS-CoV-2. Future field studies of UVGI systems could address an existing research gap and provide important information on system performance in real-world situations, particularly in the context of the current COVID-19 pandemic. This comprehensive synthesis of the scientific evidence examining the impact of UV radiation on virus transmission can be used to guide implementation of systems to mitigate airborne spread and identify priorities for future research.Trial registration PROSPERO 2020 CRD42020193968.

Construction and validation of an ultraviolet germicidal irradiation system using locally available components.

Coronavirus disease (COVID-19), the disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, is responsible for a global pandemic characterized by high transmissibility and morbidity. Healthcare workers (HCWs) are at risk of contracting COVID-19, but this risk has been mitigated through the use of personal protective equipment such as N95 Filtering Facepiece Respirators (FFRs). At times the high demand for FFRs has exceeded supply, placing HCWs at increased exposure risk. Effective FFR decontamination of many FFR models using ultraviolet-C germicidal irradiation (UVGI) has been well-described, and could maintain respiratory protection for HCWs in the face of supply line shortages. Here, we detail the construction of an ultraviolet-C germicidal irradiation (UVGI) device using previously existing components available at our institution. We provide data on UV-C dosage delivered with our version of this device, provide information on how users can validate the UV-C dose delivered in similarly constructed systems, and describe a simple, novel methodology to test its germicidal effectiveness using in-house reagents and equipment. As similar components are readily available in many hospitals and industrial facilities, we provide recommendations on the local construction of these systems, as well as guidance and strategies towards successful institutional implementation of FFR decontamination.

Ultraviolet germicidal irradiation (UVGI) for in-duct airborne bioaerosol disinfection: Review and analysis of design factors

The rapid increase in global cases of COVID-19 illness and death requires the implementation of appropriate and efficient engineering controls to improve indoor air quality. This paper focuses on the use of the ultraviolet germicidal irradiation (UVGI) air purification technology in HVAC ducts, which is particularly applicable to buildings where fully shutting down air recirculation is not feasible. Given the poor understanding of the in-duct UVGI system regarding its working mechanisms, designs, and applications, this review has the following key research objectives:•Identifying the critical parameters for designing a UVGI system, including the characterization of lamp output, behavior of the target microbial UV dose-response, and evaluation of the inactivation performance and energy consumption.•Elucidating the effects of environmental factors (air velocity, air temperature, and humidity) on the UVGI system design parameters and optimization of the in-duct UVGI design.•Summarizing existing UVGI system designs in the literature and illustrating their germicidal and energy performance in light of COVID-19 mitigation.

The antimicrobial efficacy of shielded ultraviolet germicidal irradiation in CT rooms with intense human circulation.

Computed tomography (CT) premises are one of the strategic points in the spread of hospital-acquired infections. Ultraviolet germicidal irradiation (UVGI) is an effective method that could potentially be used to purify the ambient air in them. However, it cannot be directly used in the presence of humans and, therefore, it is not operationally suitable in such units with continuous human circulation. Newer devices have been developed to purify air with more efficient and shielded UV-C sources. This study aims to assess the microbial air contamination in CT scanning rooms and investigates the efficacy and technical considerations of shielded UV-C arrays. Two shielded UVGI systems, each equipped with 15 Watt UV-C LED arrays, were tested in a very busy CT unit. Initially, a pilot study was performed to determine ambient microorganisms under routine conditions before UVGI installation, followed by three basic scenarios of UVGI use under normal and abnormal conditions: A, UVGI, with both air-conditioning (AC) and ventilation on; B, UVGI, with AC on and ventilation off; C, UVGI, with both AC and ventilation off. Ambient air was sampled in various time points before and after the initialization of UV irradiation and analyzed for colony formation. The mean total colony count in the pilot study was 1360±450 CFU/m3. Pre-UVGI colony count was 3510 CFU/m3 for Scenario A, ~10000 CFU/m3 for Scenario B and 990 CFU/m3 for Scenario C. Thirty minutes after UVGI, total colony counts in all three scenarios dropped to 30 to 70 CFU/m3. Under normal operating conditions and UVGI, the mean colony count was found as 21.4±13.5 CFU/m3 and the average efficacy of the UVGI was found as 99.39%. This study identified substantial microbial air contamination in CT scanning rooms during normal and abnormal operating conditions. UV-C LED arrays effectively eliminate these microbiological contaminants. This effect is also observed under abnormal operating conditions where no other means of ventilation or air conditioning exists.

Effect of ultraviolet germicidal irradiation of the air on the incidence of upper respiratory tract infections in kittens in a nursery.

To evaluate the effect of UV germicidal irradiation of the air on the incidence of upper respiratory tract infections (URIs) in kittens in a nursery. 4- to 8-week-old kittens admitted to a kitten nursery in 2016 and 2018. 2 UV germicidal irradiation systems (1 within the heating, ventilation, and air conditioning system and 1 attached to the ceiling) were installed in a kitten nursery. Data were collected on the number of kittens in which a URI was diagnosed by means of a physical examination. The incidence of URIs was compared between 2016, when no UV systems were used, and 2018, when the UV systems were used. The overall incidence of URIs in 2016 was 12.4 cases/100 kitten admissions and in 2018 was 1.6 cases/100 kitten admissions, a significant decrease of 87.1% between the years. A significant reduction in the incidence of URIs in kittens in a nursery was noted when the UV germicidal irradiation systems were used. Therefore, airborne transmission of feline respiratory pathogens may be more important than has been previously recognized. Ultraviolet germicidal irradiation systems that disinfect the air may be an effective adjunct to standard infection prevention and control protocols in reducing the risk of the transmission of respiratory pathogens among kittens in nurseries and shelters. However, additional studies are needed to confirm the findings reported here.

Application of the Ultraviolet Germicidal Irradiation system can reduce microbial surface contamination in ambulance compartments.

Emergency responders play a vital role in the treatment of critically ill or injured patients, as they are often the first point of contact for the patient in the healthcare setting. Emergency medical services also play a vital role in the prevention and control of the transmission of communicable diseases and ensuring best practice infection control is enacted. Lindsley et al. (2018) recently examined the efficacy and impact of an ultraviolet germicidal irradiation (UVGI) system to disinfect the interior of an ambulance. A critique of this article is presented in the following.
  

Sterilization effectiveness of in-duct ultraviolet germicidal irradiation system in liquid desiccant and indirect/direct evaporative cooling-assisted 100% outdoor air system

Microorganism contamination in air conditioning systems can cause respiratory diseases in building occupants. This study aims to evaluate and predict the performance of an in-duct ultraviolet germicidal irradiation (ID-UVGI) system to reduce the microorganisms that can grow in the liquid desiccant and indirect/direct evaporative cooling-assisted 100% outdoor air system (LD-IDECOAS). Bacteria and mold on the direct evaporative cooling (DEC) element and in the outdoor and supply air were monitored using a swab and Petrifilm for surfaces and impact sampler for air during the operation of the LD-IDECOAS. Microbial contamination was first verified in the air before performing the sterilization experiment. The experimental results showed that the airborne bacteria were reduced by 78.3% after the operation of the ID-UVGI system but there was almost no reduction in the airborne mold. The bacteria and mold concentration on the DEC surface were decreased by 99.7% and 96.7% respectively 3 h after the ID-UVGI operation. Even after the ID-UVGI system was operated for 6 days, the microbial contamination in the downstream water was not decreased significantly; the inside of the DEC element was suspected to be improperly sterilized. The operating times of the ID-UVGI system needed to inactivate the microorganisms were predicted from the UV–C intensity distribution by irradiation analysis. Legionella sp. and Penicillium sp. were predicted to be inactivated by 99.9% with UV–C exposures of 6 s and 15 min 57 s or less respectively when the LD-IDECOAS is operated at the highest air flow rate.

Evaluation of in-duct UV-C lamp array on air disinfection: A numerical analysis

In-duct ultraviolet germicidal irradiation systems are effective systems that are used in disinfection of pathogenic particles transported by air that moving through the ventilation systems. Proper design of these systems is quite important for energy consumption and disinfection performance. In this study, effect of four different lamp arrays within a square cross-section ventilation duct on the average UV dose, UV dose distribution and disinfection rate are examined by using computational fluid dynamics. The standard k-ε turbulence model and the discrete phase modeling are used to analyze airflow and particle distribution within the duct, respectively. Also, Discrete Ordinates (DO) radiation model is employed to obtain the irradiation field formed by each UV lamp. It is disclosed that UV lamp arrangement within duct significantly affects UV dose distribution.

Investigation of the effects of an ultraviolet germicidal irradiation system on concentrations of aerosolized surrogates for common veterinary pathogens.

To determine whether exposure to UV germicidal irradiation (UVGI) reduces concentrations of viable aerosolized microorganisms (attenuated strains of common veterinary pathogens) in a simulated heating, ventilation, and air conditioning (HVAC) system. 42 air samples seeded with bacteriophage MS2 or attenuated strains of Bordetella bronchiseptica, feline calicivirus, feline herpesvirus-1, canine parvovirus, or canine distemper virus (6/microorganism) or with no microorganisms added (6). A simulated HVAC unit was built that included a nebulizer to aerosolize microorganisms suspended in phosphate-buffered water, a fan to produce airflow, 2 UVGI bulb systems, and an impinger for air sampling. Ten-minute trials (3 with UVGI, 3 without UVGI, and 1 negative control) were conducted for each microorganism. Impingers collected microorganisms into phosphate-buffered water for subsequent quantification with culture-based assays. Results for samples yielding no target microorganisms were recorded as the assay's lower limit of detection. Statistical analysis was not performed. The UVGI treatment resulted in subjectively lower concentrations of viable MS2, B bronchiseptica, and canine distemper virus (arithmetic mean ± SD log10 microorganism reduction, 2.57 ± 0.47, ≥ 3.45 ± 0.24, and ≥ 1.50 ± 0.25, respectively) collected from air. Feline herpesvirus-1 was detected in only 1 sample without and no samples with UVGI treatment. Feline calicivirus and canine parvovirus were not detectable in any collected samples. Results for some surrogates of veterinary pathogens suggested a potential benefit to supplementing manual disinfection practices with UVGI-based air cleaning systems in animal care environments. Further research is needed to investigate the utility of UVGI in operating HVAC systems.

A study on the prediction of surface sterilization performance of ultraviolet germicidal irradiation system using radiance analysis

A study on the prediction of surface sterilization performance of ultraviolet germicidal irradiation system using radiance analysis