We conduct test particle simulations to examine the acceleration mechanism of relativistic electrons through interaction with multi-subpacket chorus waves. As the analysis of recent observations reveals, amplitude of a rising tone element of chorus wave consists of many short wave packets. We call this single rising tone chorus element with the collective structure of multiple short wave packets as a multi-subpacket chorus wave. In this simulation, we develop the wave model with rapidly fluctuating amplitude and phase discontinuities across each subpacket, in order to examine how these features of multi-subpacket chorus wave influence the nonlinear trapping processes in efficient acceleration of relativistic electrons such as relativistic turning acceleration (RTA) and ultra-relativistic acceleration (URA). To conduct comprehensive examinations, we test more than nine million particles with various initial conditions covering the energy range from 100 to 6 MeV, and the equatorial pitch angles from 10° to 89°. The test particles interact with a single rising tone element of multi-subpacket chorus wave set up with the maximum amplitude of about 2 nT and the frequency rise from about 1.3 kHz to 3.8 kHz over 0.25 s. Relativistic electrons are accelerated by about 160 keV under preferable conditions. The energy increase verifies the high efficiency of acceleration by the wave–particle interactions, based on the fact that it is achieved by a short time interaction less than 1 s with a single element of chorus wave. By analyzing the detailed behavior of the accelerated electrons, we find successive trapping of the resonant electrons resulting in the efficient accelerations from the consecutive multiple subpackets of a chorus wave element.