Ultra-high Performance Liquid Chromatography-quadrupole Mass Research Articles

Microalgae produced during phycoremediation of swine wastewater contains effective bacteriostatic compounds against antibiotic-resistant bacteria

Studies on the antimicrobial effects of microalgae extracts are commonly reported using algae biomass grown in sterile synthetic mineral medium and controlled laboratory conditions. However, variations in environmental conditions and culture medium composition are known to alter microalgae biochemical structure possibly affecting the type and concentrations of bioactive compounds with antimicrobial properties. In this work, solvent extracts of the microalgae Chlorella spp. were tested for antimicrobial effects against gram-positive and multidrug resistant pathogenic bacteria Staphylococcus hyicus, Enterococcus faecalis and Streptococcus suis. Microalgae was cultivated at field scale open pond reactor using raw swine wastewater as growth substrate. Dichloromethane or methanol were used to obtain the microalgae extracts. Characterization of the extracts by ultra-high performance liquid chromatography-quadrupole mass spectrometry revealed the presence of 23 phytochemicals with recognized antimicrobial properties. Bacteriostatic activity was observed in plating assays by formation of inhibition zones ranging from 7 to 18 mm in diameter. Only dichloromethane extracts were inhibitory to all three model bacteria. The minimum inhibitory concentration assessed for dichloromethane extracts were 0.5 mg mL−1 for Staphylococcus hyicus and Enterococcus faecalis and 0.2 mg mL−1 for Streptococcus suis. Bactericidal effects were not observed using solvent-extracts at 2 or 5 mg L−1. To the best of authors knowledge, this is the first report on the antimicrobial effects of Chlorella spp. extracts against Staphylococcus hyicus and Streptococcus suis. Overall, Chlorella spp. grown on swine wastewater contains several phytochemicals that could be further explored for the treatment of infections caused by antibiotic-resistant bacteria pathogens.

Simultaneous quantification of ten constituents of Xanthoceras sorbifolia Bunge using UHPLC-MS methods and evaluation of their radical scavenging, DNA scission protective, and α-glucosidase inhibitory activities

The present study was designed to investigate the bioactive constituents of Xanthoceras sorbifolia in terms of amounts and their antioxidant, DNA scission protection, and α-glucosidase inhibitory activities. Simultaneous quantification of 10 X. sorbifolia constituents was carried out by a newly established ultra-high performance liquid chromatography-quadrupole mass spectrometry method (UHPLC-MS). The antioxidant activities were evaluated by measuring DPPH radical scavenging and DNA scission protective activities. The α-glucosidase inhibitory activities were investigated by using an assay with α-glucosidase from Bacillus Stearothermophilus and disaccharidases from mouse intestine. We found that the wood of X. sorbifolia was rich in phenolic compounds with the contents of catechin, epicatechin, myricetin, and dihydromyricetin being 0.12–0.19, 1.94–2.16, 0.77–0.91, and 6.76–7.89 mg·g−1, respectively. The four constituents strongly scavenged DPPH radicals (with EC50 being 4.2, 3.8 and 5.7 μg·mL−1, respectively) and remarkably protected peroxyl radical-induced DNA strand scission (92.10%, 94.66%, 75.44% and 89.95% of protection, respectively, at a concentration of 10 μmol·L−1). A dimeric flavan 3-ol, epigallocatechin-(4β→8, 2β→O-7)-epicatechin potently inhibited α-glucosidase with an IC50 value being as low as 1.2 μg·mL−1. The established UHPLC-MS method could serve as a quality control tool for X. sorbifolia. In conclusion, the high contents of antioxidant and α-glucosidase inhibitory constituents in X. sorbifolia support its use as complementation of other therapeutic agents for metabolic disorders, such as diabetes and hypertension.