Typical Karst Region Research Articles

Exploring the geochemical characteristics, sources, influencing factors, and potential remediation strategies of Cd in a typical karst region

Exploring the geochemical characteristics, sources, influencing factors, and potential remediation strategies of Cd in a typical karst region

The past and future dynamics of ecological resilience and its spatial response analysis to natural and anthropogenic factors in Southwest China with typical Karst

With the global land use/land cover (LULC) and climate change, the ecological resilience (ER) in typical Karst areas has become the focus of attention. Its future development trend and its spatial response to natural and anthropogenic factors are crucial for understanding the changes of ecologically fragile areas to human behavior. However, there is still a lack of relevant quantitative research. The study systematically analyzed the characteristics of LULC changes in Southwest China with typical Karst over the past 20 years. Drawing on the landscape ecology research paradigm, a potential-elasticity-stability ER assessment model was constructed. Revealing the characteristics and heterogeneity of the spatial distribution, annual evolution, and development trend of ER in the past and under different scenarios of shared socioeconomic pathways and representative concentration pathways (SSP-RCP) in the future. In addition, the spatial econometric model was utilized to reveal the spatial effect response mechanism of ER, and adaptive development strategies were proposed to promote the sustainable development of Southwest China. The study found that : (1) In the past 20 years, the LULC in Southwest China showed an accelerated change trend, the ER decreased declined in general, and there was significant spatial heterogeneity, showing the spatial distribution pattern of “west is larger than east, south is larger than north, and reduction in the west was slower than that in the east.” (2) Under the same SSP scenario, with the increase of RCP emission concentration, the area of the lowest-resilience increased significantly, and the area of the highest-resilience decreased. (3) The woodland was the largest contributor to ER per unit area in the Southwest China, and grassland was the main LULC type, which had a prominent impact on the ER of the study area. (4) The average precipitation and the normalized difference vegetation index (NDVI) were significant natural drivers of ER in the study area, and the economic growth, innovation, and optimization of industrial structure contributed to the ER of Southwest China. Overall, the integration of quantitative assessment and multi-scenario-based modeling not only provides new perspectives for understanding the pattern of change and response mechanisms, but also provides valuable references for other typical Karst regions around the world to achieve sustainable development.

The Characteristics and Application of Deuterium and Oxygen Isotopes to Karst Groundwater, Southwest China

Stable isotopes in natural water bodies serve as hydrologic tracers, with a history of extensive application in karst groundwater research. The present paper is a systematic review of previous research results, with the objective of sorting out and summarizing the stable isotopic characteristics of karst groundwater in southwest China, which is the most typical karst region worldwide. Comprehensive analysis reveals that the deuterium and oxygen isotope values of most karst groundwater are characterized temporally as ‘enriched in the rainy season and depleted in the dry season’, which is the opposite of the pattern of precipitation. While the spatial distribution feature is basically consistent with the spatial variation pattern of precipitation, which is characterized as ‘depleted from the coast to the interior’. Additionally, the main applications of stable isotopes to karst groundwater are discussed, including karst groundwater recharge source and recharge elevation identification, research on karst groundwater in the hydrological cycle, the hydrological process of karst groundwater, and karst groundwater contamination tracking. Finally, we looked forward to future research on karst groundwater based on deuterium and oxygen isotopes. It is our hope that this review may provide insight into the study of karst groundwater.

The Spatiotemporal Variability of Soil Available Phosphorus and Potassium in Karst Region: The Crucial Role of Socio-Geographical Factors

The contents of soil available phosphorus (AVP) and potassium (AVK) in karstic mountainous agricultural areas have changed rapidly in recent decades. This temporal variation displays strong spatial heterogeneity due to these areas’ complex topography and anthropogenic activities. Socio-geographical factors can reflect the changes in the natural environment caused by human beings, and our objective is to enhance understanding of their role in explaining the changes of AVP and AVK. In a typical karst region (611.5 km2) with uniform soil parent material and low climatic variability, 255 topsoil samples (138 in 2012 and 117 in 2021) were collected to quantify the temporal AVP and AVK changes. Random forest (RF) and partial dependence plot analyses were conducted to investigate the responses of these changes to socio-geographical factors (distance from the nearest town center [DFT] and village density [VD]), topography, biology, and landscape pattern indexes. The mean values of AVP (48.25 mg kg−1) and AVK (357.67 mg kg−1) in 2021 were significantly (p < 0.01) higher than those in 2012 (28.84 mg kg−1 and 131.67 mg kg−1, respectively). Semi-variance analysis showed strong spatial autocorrelation for AVP and AVK, ranging from 7.29% to 10.95% and 13.31% to 10.33% from 2012 to 2021, respectively. Adding socio-geographical factors can greatly improve the explanatory power of RF modeling for AVP and AVK changes by 19% and 27%, respectively. DFT and VD emerged as the two most important variables affecting these changes, followed by elevation. These three variables all demonstrated clear nonlinear threshold effects on AVP and AVK changes. A strong accumulation of AVP and AVK was observed at DFT < 5 km and VD > 20. The AVP changes increased dramatically when the elevation ranged between 1298 m and 1390 m, while the AVK changes decreased rapidly when the elevation ranged between 1350 m and 1466 m. The interaction effects of DFT and VD with elevation on these changes were also demonstrated. Overall, this study examined the important role of socio-geographical factors and their nonlinear threshold and interaction effects on AVP and AVK changes. The findings help unravel the complex causes of these changes and thus contribute to the design of optimal soil phosphorus and potassium management strategies.

Integrated analysis of distribution characteristics, controlling factors, source apportionment, and potential remediation requirements of Pb in a typical karst region

Integrated analysis of distribution characteristics, controlling factors, source apportionment, and potential remediation requirements of Pb in a typical karst region

Geochemical characteristics, source analysis, influencing factors, and reserves of soil Selenium in Wuming, Guangxi, China.

Although selenium (Se) reserves are crucial for the development and exploitation of Se-rich resources in karst soil areas, research on these reserves is still limited. A comprehensive study was conducted in a typical karst region known for its Se richness. A total of 12,547 surface soil samples, 134 deep soil samples, and 60 soil profiles from various locations were systematically collected. The findings showed that the Se content in the surface soil ranged from 0.073 to 9.04mg/kg, with a baseline level of 0.84mg/kg. This underscores the high background level and moderate variability in the region. Surface soil Se exhibited a notable positive correlation with deep soil Se, and an inverse correlation with pH (p < 0.01). One-way analysis of variance indicated that land formations and soil structure were the primary determinants affecting the concentration of Se in the topsoil (p = 0.000), with parent rock type and land-use type following closely (p = 0.003). In addition, the study included an investigation of soil Se variations with depth using 60 soil profiles. Through this analysis, it was revealed that Se content exhibited an exponential change with depth. Multiple integrations were employed to derive formulas for calculating Se reserves in the 0-200cm depth range. Following these calculations, the estimations of Se stockpile across diverse types of source materials, varieties of soils, and land management methods were determined, highlighting the findings using a passive construction. This paper lays the groundwork for advancing the extraction and application of Se.

Quantifying the water use efficiency of karst ecosystems and response to environmental factors

Study regionThe study area is located in Guizhou province, the centre of the karst region in southwest China. Study focusAs a typical karst region, Guizhou has long faced challenges with water scarcity. Quantifying water use efficiency (WUE) is crucial for the efficient use of water resources and sustainable management. This study examined the spatio-temporal evolution patterns and its driving factors on WUE in Guizhou using Theil-Sen's slope, partial correlation methods, and the geographic detector model. New hydrological insights for the region(1) The multi-annual mean WUE of non-karst region (NKR, 2.75 gC kg−1 H2O yr−1) is higher than that of karst region (KR, 2.10 gC kg−1 H2O yr−1) during 2001–2020. (2) The rate of increase of WUE was three times higher in KR than in NKR. (3) Precipitation and temperature were identified as the key factors influencing WUE. Interactions between each environmental factors were observed to have both bi-variate and non-linear enhancement effects. Notably, in the NKR, soil moisture, vegetation type and leaf area index were the dominant factors influencing WUE, with interactions between environmental factors had both enhanced and weakened effect on WUE. (4) The synergistic effects of these interactions exceeded the individual or cumulative effects of the two environmental factors. These findings contribute to our understanding of the environmental factors driving changes in WUE in the KR.

River–Spring Connectivity and Hydrogeochemical Processes in a Karst Water System of Northern China: A Case Study of Jinan Spring Catchment

Frequent surface water–groundwater interactions and prevalent anthropogenic inputs make karst water systems vulnerable to human disturbance. As a typical karst region in North China, the Jinan Spring Catchment has become increasingly threatened due to rapid population growth and urban expansion. In this study, the local river–spring interaction and its interference with the hydrogeochemical evolution of groundwater are evaluated based on water stable isotopes and hydrochemistry. Twenty-two karst groundwater, eleven Quaternary pore water, sixteen spring water, and thirty-two surface water samples were collected during low- and high-flow conditions over the course of a year. The isotopic signatures of four different water types display significant differences, reflecting the recharge–discharge relationship of the karst water system. Mountainous springs feature lighter isotopes, whereas urban springs have significantly heavier isotopes. The result of end-member mixing analysis shows that the surface–groundwater interaction varies spatially and temporally within the spring catchment. Urban springs receive considerable replenishment from the surface water, especially after rainy episodes (up to 50%), while mountainous springs show little hydraulic dependence on surface water leakage (4~6%). Local mineral dissolution (including calcite, dolomite, gypsum, and halite), CO2 dissolution/exsolution, and cation exchange are the main hydrogeochemical processes constraining water chemistry in the spring catchment. The deterioration of water quality can be attributed to anthropogenic influences involving the discharge of domestic effluents, agricultural activities, and irrigation return flow. The findings of this work can improve our understanding of the complex karst water system and serve as a reference for sustainable groundwater management in other karst areas of northern China.

Terrestrial algae: pioneer organisms of carbonate rock solutional weathering in South China karst.

The formation of soil in karst ecosystem has always been a scientific problem of great concern to human beings. Algae can grow on the exposed and non-nutrition carbonate surface, inducing and accelerating weathering of rock substrates, thus promoting soil formation. Yet the actual contribution of algae to solutional weathering intensity remains unclear. In this study, we performed weathering simulation experiment on two algae species (Klebsormidium dissectum (F.Gay) H.Ettl & G.Gärtner and Chlorella vulgaris Beijerinck), which were screened from carbonated rock surfaces from a typical karst region in South China. The results showed: (1) both algae have solutional weathering effect on carbonate rock, (2) there is no difference of solutional intensity observed, yet the solutional modes are different, suggesting different ecological adaptative strategies, (3) algae on carbonate rocks have higher carbonic anhydrase activity (CAA) and secrete more extracellular polysaccharide (EPS), accelerating rock weathering. (4) The absolute dissolution amount of carbonate rock with algae participation is 3 times of that of without algae. These results indicate the significant impact of terrestrial algae on carbonate rock solutional weathering and provides quantitative evidence that terrestrial algae are pioneer species. It also contributes to our further understanding of soil formation in karst ecosystems in South China.

Effect of Vegetation Restoration on Soil Humus and Aggregate Stability within the Karst Region of Southwest China

This study aims to investigate the impact of vegetation restoration on soil humus and aggregate stability within the karst region of Southwest China. This study focused on soils at five vegetation succession stages (abandoned land, grassland, shrub rangeland, shrubland, and secondary forest) in the typical karst region, and the aggregate stability was determined using wet sieving and the Le Bissonnais method. Simultaneously, the Pallo method and separation extraction were used to determine the humus composition, aiming to analyze the distribution of humus content in the soil aggregates and its effect on aggregate stability. The results revealed the following: (1) The mean weight diameter of soil aggregates significantly increased with vegetation restoration stages. Soil water-stable aggregates at each vegetation stage mainly included particles over 2 mm in size. (2) The humic acid and fulvic acid contents consistently increased with vegetation restoration, and the precipitation quotient value of the humification degree showed an increasing trend. At each vegetation restoration stage, the percentage of each humus component was, from highest to lowest, as follows: insoluble HM, fulvic acid, humic acid, clay-bound HM, and iron-bound HM. (3) Through stepwise regression analysis, humic acid content in &gt;2 mm aggregates, fulvic acid and clay-bound HM contents in 1–2 mm aggregates, and insoluble HM content in &lt;0.25 mm aggregates were the dominant factors affecting soil aggregate stability in the karst region. These results aim to provide novel insights for a more in-depth comprehension of the restoration and rehabilitation of vegetation within the karst region of Southwest China, thereby laying a robust foundation for scientific theories and further investigations.

Nature's contributions to people responding to landscape stability in a typical karst region, southwest China

Understanding the response of nature's contributions to people (NCP) to change in landscape stability may constrain and guide human modification of nature. However, the effects of landscape stability on NCP, in the context of active human modification, remain unclear. To address this issue, we chose the Wujiang River Basin in the Yangtze River Economic Belt of China as a study area and quantified landscape stability in 2000, 2010, and 2020 under different scenarios. Environmental protection and comprehensive development scenarios were simulated for 2030 using the patch-generating land use simulation (PLUS) model. For current and future scenarios, NCP were assessed in terms of indicators, which included habitat creation and maintenance (NCP1), regulation of freshwater (NCP6), and protection of soils (NCP8). Under the current status quo, urban expansion was 491.547 km2, and landscape stability showed a decreasing trend, with the mean value decreasing from 0.049 in 2000 to 0.015 in 2020. In future scenarios, landscape stability was highest under the comprehensive development scenario. In addition, in terms of changes in NCP, the high-value contributions declined by 16%–48% from 2000 to 2020. Our results clarified how NCP respond to landscape stability. These results can help conservation managers develop more targeted ecological strategies that will constrain human modification activities to enhance well-being.

Pollution characteristics of peroxyacetyl nitrate in karst areas in Southwest China

In recent years, the issue of photochemical pollution in the Guangxi region of China has escalated considerably. However, there remains a notable dearth of related research in this area. Peroxyacetyl nitrate (PAN), recognized as a reliable indicator of photochemical pollution, was the focus of our study. This study marks the inaugural observation of PAN levels in Guilin, a renowned world tourist destination situated in a typical karst region, during 1–31 October 2021. Throughout this observation period, the average volume concentration of PAN ranged from 0.087 to 2.559 ppb, which was 3.61 times higher than the South China background site of the Nanling. Combined with meteorological factors and potential source analysis, the causes of a typical high-value PAN process were explored during 24–29 October. The results showed that, during this high-value PAN event, pollution primarily originated from the horizontal transport of polluted air masses and the descent of high-altitude air masses from Hunan Province in the northeast direction. Additionally, the meteorological conditions, including high temperatures, intense radiation, and low humidity, fostered local PAN formation. Notably, traffic emissions emerged as the primary source of PAN's locally generated precursor volatile organic compounds. Furthermore, we estimated the background concentration of O3 to be approximately 20.347 ppb based on PAN monitoring data, constituting 44.4% of the total O3 levels in Guilin City. This study offers valuable insights for addressing and mitigating photochemical pollution in southern Chinese cities, while providing a theoretical foundation for regional pollution control efforts.摘要桂林是世界著名的地处喀斯特地区的旅游胜地, 其光化学污染问题日益严重.过氧乙酰硝酸酯(PAN)被认为是光化学污染的可靠指标, 也是本研究的重点. 本研究于2021年10月首次观测了桂林的PAN的浓度为0.087–2.559 ppb, 同时探讨了PAN典型高值过程的成因. 此次污染主要来源于东北方向污染气团的水平和高空输送, 同时, 高温, 强辐射和低湿度等气象条件也促进了本地PAN的形成. 本研究同时估算了桂林市的O3背景浓度为20.347 ppb.这项研究为城市的光化学污染控制工作提供了理论基础.

Regarding reference state to identify priority areas for ecological restoration in a karst region

Widespread degradation of natural ecosystems around the globe has resulted in several ecological problems. Ecological restoration is considered a global priority as an important means of mitigating ecosystem degradation and enhancing ecosystem services provision. Regarding ecosystem reference state is a prerequisite for ecological restoration. However, there were few studies focusing on how to regard reference state for ecological restoration, especially under a changing climate. Taking Guizhou Province, a typical karst region in China, as a case study area, in this study we firstly assessed ecosystem services under homogeneous climate conditions. Secondly, we defined the optimal ecosystem services as ecosystem reference state, and then evaluated restoration suitability under a comprehensive framework. Finally, ecological restoration priority areas (EPRAs), which included ecological reconstruction areas, assisted regeneration areas and conservation priority areas needing restoration, were identified by integrating restoration suitability and conservation priority areas. The results showed that the services of water conservation and habitat maintenance only increased less than 10% from 2001 to 2018. Identified ecological reconstruction areas and assisted regeneration areas covered 1078 km2 and 1159 km2 respectively. Additionally, 15 conservation priority areas with the total area of 18,507 km2 were identified as conservation priority areas needing restoration. Accounting for 11.78% of the total area, ERPAs were mostly located in the eastern part of Guizhou, including Qiandongnan, Tongren, and Zunyi. The approach proposed here for regarding ecosystem reference state after controlling climate variables and the framework for identifying ERPAs can provide a scientific reference for large-scale ecological restoration planning.

The distribution, sources and health risk of N-nitrosamines in karst dominated water of Liujiang River, Southwest China

N-nitrosamines are a new class of disinfection byproducts with significant toxicity and carcinogenicity that have attracted extensive attention worldwide in recent years. N-nitrosamines in natural and drinking water would have adverse effects on people. The occurrence of N-nitrosamines in surface water, groundwater, and tap water of the Liujiang River Basin was investigated to disclose the source, fate, and cancer risk of N-nitrosamines in a typical karstic river. The origins of N-nitrosamines contamination were investigated using principal component analysis and multiple linear regression (PCA-MLRA). The results indicated that the concentrations of nine N-nitrosamines species were NDMA (1.7–342 ng/L), NMEA (ND, ND represents not detected), NPYR (2.5–53 ng/L), NDBA (ND-87 ng/L), NDEA (0.2–5.7 ng/L), NDPA (ND-34 ng/L), NPIP (ND-32 ng/L), NMOR (0.1–5.2 ng/L), and NDPhA (ND-0.3 ng/L). 71% of N-nitrosamines in the surface water samples of the Liujiang River's mainstream could be attributed to industrial and aquaculture effluents. Furthermore, 29% of N-nitrosamines could originate from domestic sewage and agricultural operations. In addition, we estimated the cancer risks associated with N-nitrosamines in groundwater and drinking water. For lifetime exposure (from birth to age 70), the average estimated carcinogenic risks of groundwater and tap water from oral intake were 7.4 × 10−5 (7.4 extra cancer cases per 100,000 persons exposed) and 9.7 × 10−5, respectively, which were greater than the US EPA standard (1 × 10−5). N-nitrosamines posed the highest cancer risk to children aged one to six. The experimental results would serve as a data foundation for environmental governance in typical karst regions.

An integrated approach combining LISA, BI-LISA, and the modified COPK method to improve groundwater management in large-scale karst areas

Groundwater protection and sustainable utilization of wells in large-scale karst areas face many challenges due to the complexity of numerous groundwater basins and aquifers. Therefore, scientific evidence is needed for groundwater management in these areas. A method combining source vulnerability, spatial autocorrelation, and bivariate spatial autocorrelation is proposed for groundwater management research. Based on the COP method (composed of flow Concentration, Overlying layers, and Precipitation regime), the COPK method is modified by replacing the sinkhole distance with sinkhole density, and incorporating land use and karst network development. The modified COPK method is used to evaluate the vulnerability of nitrate in groundwater in large-scale karst areas. Guizhou Province in China, which is a typical karst region, was evaluated as a study area. Comparatively to the classical COP method, the revised vulnerability map shows more areas of high vulnerability, mainly in the middle of the study area. According to the receiver operating characteristic curve (ROC) analysis, the modified method had a higher accuracy than the classical COP algorithm. Based on the accurate vulnerability index, vulnerability was classified using the natural break method, and the spatial autocorrelation of vulnerability clustering characteristics was studied. The source vulnerability was divided into High-High (HH), High-Low (HL), Low-High (LH), Low-Low (LL), and Not Significant (NS) clusters. This result can clarify the vulnerability clustering of groundwater in specific areas, thereby helping to improve groundwater management accuracy and efficiency. In addition, bivariate spatial autocorrelation was used to study the nitrate content of wells and source vulnerability. The bivariate local Moran's index showed a spatial negative correlation between the two. Similarly, the BI-LISA map can be used to characterize the spatial aggregation of nitrate content and well vulnerability. This result can help groundwater managers develop reasonable measures to protect water wells.

Influencing Factors of Elevated Levels of Potentially Toxic Elements in Agricultural Soils from Typical Karst Regions of China

Agricultural soils originating from carbonate rocks within karst regions exhibit inherent high concentrations of potentially toxic elements (PTEs) due to geogenic processes. However, the influencing factors of the elevated levels of PTEs in the naturally contaminated karst regions remain inadequately comprehended. This research investigates the chemical compositions of 278 soils derived from carbonate rocks. Descriptive statistics, stepwise multiple regression, and the random forest (RF) method were applied to screen the significant factors that affect the distribution, migration, and enrichment of the PTEs in soils. Cadmium (Cd) and arsenic (As) are the most highly contaminated PTEs in the soils of the study area, and the average contents of Cd and As in soils are 11.5 and 2.92 times the national soil background value, respectively. The pollution risk ranking of PTEs is Cd &gt; As &gt; Cr &gt; Zn &gt; Pb &gt; Cu &gt; Ni &gt; Hg, using the proportion exceeding the risk screening thresholds of agricultural land as the standard. Soil Fe2O3 and Mn contents, soil pH and total organic carbon (TOC) values, and weathering intensity (characterized using the chemical index of alternation, CIA) are the most important factors influencing the PTE levels in agricultural soils.

Ecological differentiation and assembly processes of abundant and rare bacterial subcommunities in karst groundwater.

The ecological health of karst groundwater has been of global concern due to increasing anthropogenic activities. Bacteria comprising a few abundant taxa (AT) and plentiful rare taxa (RT) play essential roles in maintaining ecosystem stability, yet limited information is known about their ecological differentiation and assembly processes in karst groundwater. Based on a metabarcoding analysis of 64 groundwater samples from typical karst regions in southwest China, we revealed the environmental drivers, ecological roles, and assembly mechanisms of abundant and rare bacterial communities. We found a relatively high abundance of potential functional groups associated with parasites and pathogens in karst groundwater, which might be linked to the frequent regional anthropogenic activities. Our study confirmed that AT was dominated by Proteobacteria and Campilobacterota, while Patescibacteria and Chloroflexi flourished more in the RT subcommunity. The node-level topological features of the co-occurrence network indicated that AT might share similar niches and play more important roles in maintaining bacterial community stability. RT in karst groundwater was less environmentally constrained and showed a wider environmental threshold response to various environmental factors than AT. Deterministic processes, especially homogeneous selection, tended to be more important in the community assembly of AT, whereas the community assembly of RT was mainly controlled by stochastic processes. This study expanded our knowledge of the karst groundwater microbiome and was of great significance to the assessment of ecological stability and drinking water safety in karst regions.

Impact of Future Climate and Land Use Changes on Runoff in a Typical Karst Basin, Southwest China

Climate change and land use change are the two main factors affecting the regional water cycle and water resources management. However, runoff studies in the karst basin based on future scenario projections are still lacking. To fill this gap, this study proposes a framework consisting of a future land use simulation model (FLUS), an automated statistical downscaling model (ASD), a soil and water assessment tool (SWAT) and a multi-point calibration strategy. This frameword was used to investigate runoff changes under future climate and land use changes in karst watersheds. The Chengbi River basin, a typical karst region in southwest China, was selected as the study area. The ASD method was developed for climate change projections based on the CanESM5 climate model. Future land use scenarios were projected using the FLUS model and historical land use data. Finally, the SWAT model was calibrated using a multi-site calibration strategy and was used to predict future runoff from 2025–2100. The results show that: (1) the developed SWAT model obtained a Nash efficiency coefficient of 0.83, which can adequately capture the spatial heterogeneity characteristics of karst hydro-climate; (2) land use changes significantly in all three future scenarios, with the main phenomena being the interconversion of farmland and grassland in SSPs1-2.6, the interconversion of grassland, farmland and artificial surfaces in SSPs2-4.5 and the interconversion of woodland, grassland and artificial surfaces in SSPs5-8.5; (3) the average annual temperature will show an upward trend in the future, and the average annual precipitation will increase by 11.53–14.43% and (4) the future annual runoff will show a significant upward trend, with monthly runoff mainly concentrated in July–September. The variability and uncertainty of future runoff during the main-flood period may increase compared to the historical situation. The findings will benefit future water resources management and water security in the karst basin.

Variations in litter-soil properties between planted and naturally restored forests drive microbial community structure and function

How forest restoration can affect soil microbial composition and functionality remains unclear, limiting our ability to evaluate restoration approaches. Here, we used metagenomes, 16S rRNA, and ITS gene sequencing to distinguish the composition and functional attributes of soil microbial communities between plantation forest of Pinus massoniana (PF) and naturally restored secondary forest (SF) in their organic horizon and mineral soil from a typical karst region in Southwest China. Our results showed that fungal communities differed more than bacterial communities between PF and SF, while the differences in archaeal communities were not significant. The most abundant fungal phylum in both mineral soil and organic horizon was Ascomycota at SF and Basidiomycota at PF, respectively. The PF soil had lower nitrogen (N) cycling gene abundance and lower inorganic N availability compared to SF soil probably because of the poor soil and lower litter quality. The SF soil had higher abundances of genes associated with labile carbon (C) (i.e., starch, hemicellulose, cellulose, pectin, and chitin) degradation compared with PF soil, but lower abundances of genes responsible for recalcitrant C (i.e., lignin and aromatics) degradation, which was strongly correlated with the litter properties. Simultaneous effects of soil and litter variables explained >50 % of the variation in microbial community structure and functional gene composition; soil nitrate-N had the strongest effect on the soil bacterial and fungal community structure. Our results highlight the superiority of naturally restored secondary forests for improving both soil fertility and soil microbial-mediated C and N cycling functions, which can support forest managers in selecting forest restoration approaches that optimize C and N storage in forest stands.

Zn isotope signatures in soil Fe[sbnd]Mn nodules with karst high geochemical background

Zn isotope has the potential to be used as an environmental tracer, due to its role in fingerprinting specific sources and processes. However, few studies have focused on Zn isotope system in terrestrial ferromanganese (FeMn) nodules, which is important on understanding the behaviors of Zn in soils. In this study, we analyse the isotopic composition in soil FeMn nodules and surrounding materials from a typical karst region in Guangxi Province, southwestern China and use advanced synchrotron-based methods to characterize Zn speciation. The Zn isotope compositions of the FeMn nodules range from 0.09 to 0.66 ‰, with an average value of 0.24 ‰. Pb isotope fingerprinting reveals that the major material sources contributing to the FeMn nodules are the surrounding soil (δ66Zn: ~0.36 ‰) and partly weathered carbonate bedrock (δ66Zn: ~0.58 ‰), which contain heavier Zn isotopes than the nodules. Synchrotron-based X-ray fluorescence (μ-SXRF) shows that Zn is well correlated with both Fe and Mn. X-ray absorption near edge spectroscopy (XANES) measurements reveal that Zn is associated with both goethite and birnessite phases, with goethite-sorbed Zn accounting for ~76 % of the total Zn and birnessite-sorbed Zn accounting for ~24 %. By combining these new results, the isotopically light Zn in the FeMn nodules compared to their sources can be explained by equilibrium sorption of Zn on goethite and birnessite, during which light Zn is preferentially sorbed. Our study provides important new data on Zn isotope compositions in terrestrial soil FeMn nodules and constrains associated mechanisms, and have implications for using Zn isotopes as environmental tracers.