Insertion Type Research Articles

Influence of the retropreparation ultrasonic insert type on the adhesive union of fiberglass posts within the root canal

To assess the displacement resistance of cemented fiberglass posts within the root canal, based on the type of ultrasonic retropreparation insert used during endodontic surgery. Material and methods: Thirty primary bovine incisor roots, measuring 17 mm in length, underwent endodontic treatment. After a seven-day period, the teeth were debrided and prepared to receive 13 mm posts. The roots were randomly divided into three groups, depending on the type of ultrasonic retropreparation insert used: GC – no retropreparation (control); GL – smooth insert; GD – diamond insert. The posts were cemented using RelyX U200® within the root canal. Subsequently, the roots were immersed in distilled water for 15 days and then sectioned to create specimens with an average thickness of 1.87 mm in each of the root thirds (cervical and middle). These specimens were subjected to a push-out test. Following the test, the fractured samples were examined under a stereomicroscope to determine the fracture pattern. The data obtained were analyzed using a one-way Anova test and non-parametric t-test (α = 0.05). Results: There were no statistically significant differences in the cervical third when comparing the tested ultrasonic inserts. However, in the analysis of the middle third, the use of a smooth ultrasonic insert had a significant impact on the adhesion of the intraradicular post (P < 0.05). Conclusion: The application of a smooth ultrasonic insert during endodontic retropreparation significantly influenced the displacement resistance of intraradicular fiberglass posts cemented in the middle third of the canal. In the cervical third, the type of ultrasonic retropreparation insert used did not affect post retention.

Effect of gluing garment materials with adhesive inserts on their multidirectional drape and bending rigidity

Abstract The article concerns the study on multidirectional drape and bending rigidity of six wool (clothing) fabrics with two types of adhesive inserts. The aim of research was to investigate the effect of joining woolen fabrics with “Freudenberg Vilene” adhesive inserts on the bending rigidity and multidirectional drape. The adhesive inserts were differentiated according to mass per square meter, while the fabrics were differentiated according to thickness, weave, and mass per square meter. Based on the scientific literature, the issues related to the methods of testing the bending rigidity and multidirectional drape coefficient were discussed. All the conducted tests were aimed at the proper selection of adhesive inserts for the designed clothing products. The use of adhesive inserts affects the bending stiffness and multidirectional drapeability of clothing material. The fabric weave significantly affects the values of bending rigidity and drapeability. As the thickness of adhesive inserts increases, the overall bending rigidity increases. The greater the fabric mass per square meter, the stiffer the fabric. As the mass per square meter and thickness increase, the multidirectional rigidity of fabrics increases. The value of bending rigidity for the plain fabric is higher compared to twill fabric.

CRISPR/Cas9-Mediated BocPDSs Gene Editing in Chinese Kale Using the Endogenous tRNA-Processing System

Chinese kale is a native vegetable from the Brassicaceae family that is grown extensively in Southeast Asia and Southern China. Its low genetic transformation and gene editing efficiency hinder gene function research and molecular biology in Chinese kale. CRISPR/Cas9 is a useful tool for plant genome research due to its rapid development and optimization. This study targeted BocPDSs, (BocPDS1, BocPDS2) to establish an effective CRISPR/Cas9 system in Chinese kale. A tandemly arranged tRNA-sgRNA construct was used to express numerous sgRNAs to induce BocPDS1 and BocPDS2 double and single mutations, with a mutation rate of 61.11%. As predicted, several mutant plants showed an albino phenotype with a harbored mutation in an exon and intron region, highlighting the relevance of the intron. The presence of mutations in the intron region suggests that the cleavage process in Chinese kale, utilizing CRISPR/Cas9 shows a preference for AT-rich regions. The distinct and somewhat redundant functions of BocPDS1 and BocPDS2 are demonstrated by the complete albino phenotype of the double mutants and the mosaic albino phenotype of the individual BocPDS1 and BocPDS2 mutants. Specific gene editing modes, including base deletion, base substitution, and base insertion, were identified in the sequence of the target gene. Among them, short nucleotide insertions were the most common type of insertion, with base insertions having the highest frequency (61.54%). Furthermore, no instances of off-target gene editing were detected. The current work demonstrated that the CRISPR/Cas9 gene editing system, which relies on endogenous tRNA processing, can effectively induce mutagenesis in Chinese kale. This finding establishes a theoretical basis and technical backbone for the more effective implementation of CRISPR/Cas9 gene-editing technology in Chinese kale and Brassica plants.

Structure-based clustering and mutagenesis of bacterial tannases reveals the importance and diversity of active site-capping domains.

Tannins are critical plant defense metabolites, enriched in bark and leaves, that protect against microorganisms and insects by binding to and precipitating proteins. Hydrolyzable tannins contain ester bonds which can be cleaved by tannases-serine hydrolases containing so-called "cap" domains covering their active sites. However, comprehensive insights into the biochemical properties and structural diversity of tannases are limited, especially regarding their cap domains. We here present a code pipeline for structure prediction-based hierarchical clustering to categorize the whole family of bacterial tannases, and have used it to discover new types of cap domains and other structural insertions among these enzymes. Subsequently, we used two recently identified tannases from the gut/soil bacterium Clostridium butyricum as model systems to explore the biochemical and structural properties of the cap domains of tannases. We demonstrate using molecular dynamics and mutagenesis that the cap domain covering the active site plays a major role in enzyme substrate preference, inhibition, and activity-despite not directly interacting with smaller substrates. The present work provides deeper knowledge into the mechanism, structural dynamics, and diversity of tannases. The structure-based clustering approach presents a new way of classifying any other enzyme family, and will be of relevance for enzyme types where activity is influenced by variable loop or insert regions appended to a core protein fold.

Prevalence and Pattern of Labial Frenal Attachments in Adult Nigerians - a Pilot Study

Introduction The frenum is a band of mucous membrane which connects the lips and cheeks to the alveolar mucosa, underlying periosteum, or the gingiva with a primary function of stabilizing the lips and tongue. There are variations of frenal attachment and morphologies, some associated with health, others resulting to loss of periodontium. This study therefore aims to assess the various attachments and morphologies within our study population. Materials and Methods This was a descriptive cross-sectional study conducted at the Oral Diagnosis and Periodontology clinics of the Lagos University Teaching Hospital Idi-Araba among participants who reported to both clinics. Convenience sampling method was employed in participants’ selection, and interviewer-administered questionnaires were used for data collection and documentation of socio-demographic variables, and findings of intra-oral examination. Ethical approval was obtained from the institutional Health Research Ethics Committee and informed consent was obtained from each participant. Data was analyzed using the Statistical Product and Service Solutions (SPSS) version 27. P values < 0.05 were statistically significant. Results The study comprised 337 participants, 203 (60.2%) were females. Mean age was 34.6±15.2 years. The frenal attachment distribution in the maxilla was mucosal 204 (60.5%), gingival 105 (31.2%), papillary 23 (6.8%), and papillary penetrating 5 (1.5%);while in the mandible, 233 (69.1%), 92 (27.3%), 9 (2.7%) and 3 (0.9%) presented with mucosal, gingival, papillary, and papillary penetrating frenal attachment respectively. Simple frenum was the most prevalent frenum morphology in the maxilla [328 (97.3%)] and the mandible [321 (95.2%)], followed by trifid frenum in both maxilla [5 (1.5%)] and mandible [4 (1.2%)]. Diastema was present in 27.6% in maxilla and 11.0% in the mandible. Conclusion In this study, mucosal type was predominant among all age groups. Males presented more with gingival and mucosal type of frenal insertion, while females presented more with papillary type of frenal insertion. Papillary and/or papillary penetrating were significantly associated with the presence of diastema.

Comparing results of midline catheter and peripherally inserted central catheter in cancer patients under chemotherapy.

Peripherally inserted central catheters (PICC), and peripherally inserted midlines (MC) represent a possible less invasive alternative option to totally implantable vascular access devices (TIVAD) in cancer patients. This study aims to investigate the outcomes of PICC, and MC devices in patients undergoing chemotherapy. A prospective non-concurrent observational study was conducted at the IRCCS Ospedale Policlinico San Martino, Genova (Italy) on 559 cancer patients candidate to chemotherapy bearing PICC, and MC during the period 2019-2022. The primary endpoint was the comparative analysis for failure of the different types of peripheral insertion accesses requiring removal. Secondary outcome measures were age at diagnosis, gender, period of diagnosis, cancer site and type of chemotherapy. The negative binomial regression analysis was applied to estimate catheter removal rate ratios (RR), along with 95% confidence limits (95% CL), in each category of the risk factors. The median follow-up time was 2.6 months (IQR = 1.4-4.6; min-max = 0.03-12.7), and at the end of the study period, a total of 45 catheter failures were detected. A risk reduction of approximately 75% (RR = 0.24; 95% CL = 0.11-0.51) was emphasized for patients with PICC when compared with those with MLC. Type of chemotherapy and cancer site appeared to be important predictive factors for catheter removal risk. For cancer patients undergoing chemotherapy by a non TIVAD, PICC must be preferred to MC because of higher safety and efficacy.

Maximizing efficiency in C45 steel machining: an integrated AI-based approach to coated insert optimization

AbstractMachining involves the subtraction of the material from the sample workpiece to achieve the desired shape or surface. This versatile method is capable of producing a wide range of parts, varying from simple to intricate profiles. Coating materials are increasingly being utilized in tool inserts in the production industry owing to their superior thermal properties and wear resistance. The shielding of hard coatings, with thicknesses of only a few microns, enhances performance and durability. In this study, machining of C45 steel using distinct coated inserts was explored. The experimental trials employed PVD and CVD methods for coated tungsten carbide (WC) tools/inserts and PVD-coated cermet tools/inserts with different machining parameters. Performance metrics, such as the surface finish and reliability of the tool, were considered for the evaluation. The average tool life variation between the PVD-coated cermet and PVD-coated WC was 178.86%, and 30.11% between the PVD-coated cermet and CVD-coated WC at 1 mm DOC. ANOVA was performed using Response Surface Methodology to explore the influence of input parameters on output. The results indicate that the depth of machining and spindle speed significantly influence Ra, whereas spindle speed and type of tool insert have a considerable impact on the life span of the tool. The developed mathematical model for the prediction of tool life and Ra indicates its potential for performance forecasting during C45 steel machining. Grey relation analysis was employed to optimize the process parameters. Optimal results were achieved with a spindle speed of 400 m/min, 0.5 mm depth of cut, and cermet tool inserts. PVD-coated WC inserts performed better. ANFIS was applied for the prediction and optimization of the machining parameters.

Variants in the superior attachment of uncinated process and their corresponding frontal sinus drainage pattern: a computed tomography-based retrospective clinical study

Background: Understanding the morphology and spatial relationships of the uncinate process and its associated spaces is crucial for diagnosing and treating conditions affecting sinus drainage and ventilation. This consistent anatomical structure is an important surgical landmark for endoscopic sinus surgery. Methods: Computed tomography (CT) records of 100 patients over two years were extracted via the hospital's PACS system. The type of superior insertion of uncinate process (UP) was observed in coronal cuts of CT scan images, and classification was done based on the Friedmann and Landsberg classification system and tabulated. The corresponding variations in the frontal sinus outflow tract were noticed, and their effect on frontal sinusitis was recorded. Sinusitis of the frontal sinus was in guidance with EPOS 2020 (mucosal changes within the ostiomeatal complex or sinus). Results: Type 2 was the most common of uncinate attachments (39.5%). The least common variant was type 5 (4%). Medial-type frontal sinus drainage pathway was observed in 78.5% of patients. Sinusitis was present in 23.5% of our study population. Type 2 had a 25% contribution to the occurrence of sinusitis. The medial frontal sinus drainage pathway contributes 24% to sinusitis with a significant p value of 0.010. Conclusions: Uncinate process, with its varying superior attachment, influences the frontal sinus outflow tract and may contribute to the development of frontal sinusitis. Awareness of the possible variations in the attachment of the uncinate process is vital in the preoperative planning process, which will serve as a road map to surgeons and aid in preparedness for intraoperative complications.

Improving the Performance of the Machining Process by Using Ultra‐Advanced Tools in a Clean Turning of Inconel 686 Using the Minimum Quantity Lubrication Method

ABSTRACTThe high tensile strength and high resistance of nickel‐based superalloy 686 against high temperatures and corrosion rates have made it a widely used in important applications such as the aerospace industry, high pollution‐ and corrosion‐resistance equipment manufacturing and petrochemical industry. Therefore, the machining of this advanced alloy with its unique properties is extremely important and can be challenging. Significant increase in input parameters levels, reduction of machining costs, improvement of surface and subsurface properties and clean production are among the issues that should be considered in dealing with Inconel 686 turning operations. Simultaneous application of advanced tools such as polycrystalline diamond (PCD) and polycrystalline cubic boron nitride (PCBN) and optimised minimum quantity lubrication (MQL) method and evaluating the results obtained with a wide range of output parameters related to machining process performance and tribological properties can be proposed as an innovation and a solution to this problem in this article. This study analyses several output parameters with different speeds and feeds to evaluate the effect of cutting insert type on machining process performance and tribological properties. The output parameters include tool wear, residual stress, cutting zone temperature, surface smoothness, machining forces and workpiece surface defects. The results indicated that using the optimised MQL method reduces the size of lubricant droplets and increases the surface covered by cooling. With these changes, the performance of the machining process and the parameters related to the surface integrity increase significantly. Among the parameters associated with the performance of the machining process, the PCD tool reduces the cutting zone temperature by 23%, the tool wear by 19% and the machining forces by 18% compared to the PCBN tool. In the parameters related to surface integrity, this method reduces the residual stress by 19% and the surface roughness by 9% compared to the PCBN tool. From the production index perspective, the PCD tool can significantly increase the cutting speed and feed rate, reducing production time and costs.

Self-perception of oral health, gender and employment among Brazilian population.

Self-perception of oral health is a recognized indicator, based on a single question. Association among oral health and socioeconomic status has been well established, however in Brazil, no results were found on the relation with type of insertion in labor market. The aim of this study was to describe the prevalence of negative self-perception of oral health and associated factors, according to gender among Brazilian workers population, in 2019. The National Health Survey, nationwide household-based survey, carried out in 2019, was used as source of data. Absolute and relative frequencies were calculated to understand the distribution of variables of interest and characterize the sample (n = 54,343). Binary logistic regression was used in the analyses. Odds ratio (OR) was estimated using a 95% confidence interval in bivariate and multivariate analyses. The prevalence of negative self-perception of oral health was 29.3%; in that, 31.0% among men and 27.2% among women. Compared to formal employees, the chance of negative self-perception of oral health among the informal workers was almost 20% higher among men and 13% among women after adjusting for all confounding factors. Unemployed women were 33% more likely to have negative self-perception of oral health. Self-reported unemployment increased the chance of a negative self-perception of oral health among women; however, the same effect was not observed among men. Why is there a difference? The association between informal work and negative SEOH is relevant in the Brazilian context of work deregulation and growth in the number of people without access to formal employment. The results suggested addressing employment conditions in adult oral health promotion actions, with emphasis on gender differences.

Intra-Host Citrus Tristeza Virus Populations during Prolonged Infection Initiated by a Well-Defined Sequence Variant in Nicotiana benthamiana.

Due to the error-prone nature of viral RNA-dependent RNA polymerases, the replication of RNA viruses results in a diversity of viral genomes harboring point mutations, deletions, insertions, and genome rearrangements. Citrus tristeza virus (CTV), a causal agent of diseases of economically important citrus species, shows intrinsic genetic stability. While the virus appears to have some mechanism that limits the accumulation of single-nucleotide variants, the production of defective viral genomes (DVGs) during virus infection has been reported for certain variants of CTV. The intra-host diversity generated during plant infection with variant T36 (CTV-T36) remains unclear. To address this, we analyzed the RNA species accumulated in the initially infected and systemic leaves of Nicotiana benthamiana plants inoculated with an infectious cDNA clone of CTV-T36, which warranted that infection was initiated by a known, well-defined sequence variant of the virus. CTV-T36 limited the accumulation of single-nucleotide mutants during infection. With that, four types of DVGs-deletions, insertions, and copy- and snap-backs-were found in all the samples, with deletions and insertions being the most common types. Hot-spots across the genome for DVG recombination and short direct sequence repeats suggest that sequence complementarity could mediate DVG formation. In conclusion, our study illustrates the formation of diverse DVGs during CTV-T36 infection. To the best of our knowledge, this is the first study that has analyzed the genetic variability and recombination of a well-defined sequence variant of CTV in an herbaceous host.

A Review of Central Vein Catheter for Hemodialysis: Insertion Sites and Techniques

Chronic kidney disease (CKD) is a global epidemic, being increasingly prevalent in both developed and developing countries, including Indonesia. Hemodialysis (HD) is the primary renal replacement therapy, along with peritoneal dialysis and kidney transplantation. The effectiveness of HD relies on well-functioning vascular access, such as central vein catheters (CVCs). These catheters are classified by their duration of use: short-term, medium-term, and long-term. They are also distinguished by insertion type (central or peripheral), insertion site (jugular, subclavian, femoral), and number of lumens (single, double, triple). The preferred site for insertion is the internal jugular vein, followed by the femoral vein and the subclavian vein. Techniques for CVC insertion include the central approach, the posterior approach, and others. This article reviews the role of CVC for vascular access in HD. Specifically, various CVC insertion sites and techniques will be examined. The authors will also discuss the available research on the application of CVC as vascular access for HD in Indonesia.

Multivendor comparison of quantification accuracy of effective atomic number by Dual-Energy CT: A phantom study

PurposeOur study aimed to compare the accuracy of the effective atomic number (Zeff) of five dual-energy CT (DECT) from three vendors and different generations under different scanning parameters. MethodsZeff accuracy of five DECT scanners with twelve tube voltage configurations was evaluated by using the TomoTherapy cheese phantom. The potential dose dependence of the Zeff was investigated using three radiation dose (5, 15, and 25 mGy), and the robustness of Zeff was simulated for different organs of the body by placing the inserts at different positional depths. Bias and mean absolute percentage error (MAPE) were used to characterize the accuracy of Zeff. Data underwent analysis using one-way ANOVA, followed by the Turky and LSD post hoc tests, simple linear regression, and linear mixed models. ResultsAll tube voltage configurations had a bias of less than 1. Dual layer detector DECT (dl-DECT) −140 kV has the lowest MAPE (1.79 %±1.93 %). The third generation dual source DECT (ds-DECT) and the second generation rapid switch DECT (rs-DECT) have higher MAPE than their predecessor DECT. The results of the linear mixed model showed that tube voltage configuration (F=16.92, p < 0.001) and insert type (F=53.26, p < 0.001) significantly affect the MAPE. In contrast, radiation dose only has a significant effect on the MAPE of rs-DECT. The inserts position does not affect the final MAPE. ConclusionWhen scanning different inserts, Zeff accuracy varies by vendor and DECT generation. Of all the scanners, dl-DECT had the highest Zeff accuracy. Upgrading DECT generation doesn’t lead to higher accuracy, or even lower.

Survivorship of total knee arthroplasty in poliomyelitis patients: long-term results from the R.I.P.O. registry and single-institution retrospective study.

The survival of total knee arthroplasty (TKA) in patients with poliomyelitis remains a debated topic due to the high recurrence of postoperative genu recurvatum. This study aims to report the long-term survival of TKA in patients with poliomyelitis, using data from the Italian Register of Prosthetic Implantology. A registry-based population study was conducted, utilizing data from the Emilia Romagna orthopedic arthroplasty implants registry (RIPO - Registro Implantologia Protesica Ortopedica). The cohort consisted of 71 patients with poliomyelitis-related arthritis who underwent TKA. The study assessed and analyzed demographic data, implant type, fixation method, insert type, and level of constraint. Additionally, variations in preoperative and postoperative both clinical and functional Knee Society Scores (KSS) were collected. Eight implants required revision surgery (16%), and three patients died (6.1%), resulting in a 10-year survival rate of 86.6% and a 15-year survival rate of 53.9%. Aseptic loosening was the primary cause of revision, accounting for 37.5% of failures, followed by insert wear (25%). No statistically significant correlation was found between the level of constraint and implant survival (p=0.0887, log-rank). Both the clinical and functional KSS improved postoperatively. TKA is a viable alternative to knee arthrodesis and, in properly selected patients, might represent the first-choice treatment for articular degeneration due to its high survivorship. Despite the complexity of these cases, TKA can effectively alleviate articular pain, instability, and angular deviation, thereby preserving knee functionality.

The study of perturbation in magnetized Schwarzschild anti-de Sitter spacetime and dark energy profile

AbstractWe are going to investigate the geodesic motion of charged particles in the vicinity of Schwarzschild anti-de-Sitter (S-AdS) spacetime with topological defects that admit temporal perturbation. We used the approximate Noether symmetry equation to insert the time conformal factor in the black hole without losing its symmetry structure. This type of insertion is necessary because the black hole radiates its energy and momentum in the form of gravitational waves and Hawking radiation. Along with the temporal perturbation, the S-AdS black hole (BH) is immersed in an external magnetic field. We conduct an in-depth examination of the dynamics of charged particles near a weakly magnetized and time conformal S-AdS BH. Our analysis involves calculating the shift in the position of the innermost circular orbit (ISCO) caused by both temporal perturbation and the presence of a magnetic field. Furthermore, we explore the influence of dark energy (DE) and angular momentum on the stability of these orbits. Additionally, we determine the effective force and escape velocity for a charged particle orbiting around the perturbed magnetized S-AdS BH. The application of time-dependent perturbation theory can extend our understanding to investigate the quasinormal modes (QNMs) of BH mergers.

A highly stable insertion type V1-xTixP solid solution as an anode material for high-rate lithium-ion batteries

As electric vehicles (EVs) grow in popularity, the demand for lithium-ion batteries (LIBs) simultaneously grows, and the fast-charging capability is becoming increasingly important. However, it is generally believed that graphite anode still suffers from a poor rate capability for fast-charging applications, and other insertion-type oxide anodes with a high rate capability exhibit too high Li+-ion insertion/extraction potential, which cannot meet a requirement for high energy density anodes. In this work, the hexagonal structure of V1-xTixP (x = 0.0, 0.25, and 1.0) phases were introduced as insertion-type anode materials for LIBs with a low reaction potential of Li+-ion insertion/extraction. The crystal structure and the corresponding electrochemical reaction mechanism of insertion-type anodes of TiP, VP, and MoP were compared. A facile strategy forming a substitutional solid solution is suggested to improve the electrochemical properties of anode materials by combining the end members among insertion-type VP, TiP, and MoP as forming V1-xTixP and V1-xMoxP compounds. V1-xTixP (x = 0.25) electrode, substituting V atoms with Ti atoms in VP structure, shows excellent long-term cycle stability at 1 A/g with a cycle retention of 87.7 % during 2500 cycles, delivering a two times higher capacity of 211.1 mAh g−1 compared to that of the commercial graphite electrode.

Comparison of postural control and space perception outcomes between robotic and conventional cochlear implantation in adults.

The aim of the study is to capture the difference between the groups in direct relation to the type of electrode array insertion during cochlear implantation (CI). The robotic insertion is expected to be a more gently option. As recent studies have shown, there is a difference in perception of visual vertical (SVV) and postural control related to the CI. We assume that there can be differences in postural control and space perception outcomes depending on the type of the surgical method. In total, 37 (24 females, mean age ± SD was 42.9 ± 13.0) candidates for CI underwent an assessment. In 14 cases, the insertion of the electrode array was performed by a robotic system (RobOtol, Colin, France) and 23 were performed conventionally. In all of these patients, we performed the same examination before the surgery, the first day, and 3weeks after the surgery. The protocol consists of static posturography and perception of visual vertical. The both groups, RobOtol and conventional, responded to the procedure similarly despite the dissimilar electrode insertion. There was no difference between two groups in the dynamic of perception SVV and postural parameters. Patients in both groups were statistically significantly affected by the surgical procedure, SVV deviation appeared in the opposite direction from the implanted ear: 0.90° ± 1.25; -1.67° ± 3.05 and -0.19° ± 1.78 PRE and POST surgery (p < 0.001). And this deviation was spontaneously adjusted in FOLLOW-UP after 3weeks (p < 0.01) in the both groups. We did not find a significant difference in postural parameters between the RobOtol and conventional group, even over time. Although the robotic system RobOtol allows a substantial reduction in the speed of insertion of the electrode array into the inner ear, our data did not demonstrate a postoperative effect on vestibular functions (SVV and posturography), which have the same character and dynamics as in the group with standard manual insertion. The project is registered on clinicaltrials.gov (registration number: NCT05547113).

Molecular and Biological Characteristics of a Peach Latent Mosaic Viroid PC Isolate in Peach from China: Base Mutations in Hairpin Stems and Implications for Symptomatology.

Peach latent mosaic viroid (PLMVd) infects peach trees in China and induces a conspicuous albino phenotype (peach calico, PC) that is closely associated with variants containing a 12-to-14 nucleotide hairpin insertion capped by a U-rich loop. Initially, PC disease distribution was limited to parts of Italy, and it was first detected in the field in China in 2019. To explore the molecular and biological characteristics of PLMVd PC isolates in peach in China, we conducted a comprehensive analysis of disease phenotype development and investigated the data-associated pathogenicity and in vivo dynamics of the Chinese isolate PC-A2 using slash-inoculation into GF-305 peach seedlings. Inoculated seedlings displayed PC symptoms much earlier following topping treatment, and PLMVd infectivity was further assessed using bioassay and semiquantitative RT-PCR experiments. Evolutionary analysis showed that the PC isolate and its progeny variants clustered into a single phylogroup distinct from reference PC-C40 isolates from Italy and PC-K1 and PC-K2 from South Korea. Some PC-A2 progeny variants from green leaves of PC-expressing seedlings showed unbalanced point mutations in hairpin stems compared with the PC-C40 reference sequence and constituted a new stem insertion type. The results reveal associations between the recessive phenotypes of peach albino symptoms and base variation in hairpin stem insertions relative to the PC-C40/chloroplastic heat shock protein 90 reference sequence.

TEACHER’S CODE-MIXING IN AN ENGLISH CLASSROOM AT A PRIVATE VOCATIONAL SCHOOL

This study aims to analyse the type of code-mixing that occurs by English teachers when teaching English lessons, and find out the reasons for the use of code-mixing. This research uses descriptive qualitative research. The object of this research is one English teacher in Private Vocational School in Bogor. The process of collecting the data, the researcher recorded the teacher's voice audio when teaching in class and also when conducting interviews. The data analysis technique used Muysken's theory. In this study, it was found that there were three types of code mix used by the teacher when teaching, namely the type of insertion, alternation, and lexical congruence. The use of insertion type is about 55%, then alternation 44%, and lexical congruent 1%, from 158 total utterances obtained. The results found in this study are that teachers more often use code-mixing insertion type when teaching English. Then three reasons for the use of code-mixing by the teacher, namely because of the background of a major school, for students' understanding of the material, and the existence of limited student understanding in English.

Assessment of an AI-based tool for population-wide collection of placental morphological data

ObjectivesAutomated placental assessment could allow accurate and timely morphological/pathological measurements at scale. We undertook a pilot study using an artificial intelligence-based assessment system (AI-PLAX) to ascertain the potential of a state-wide rollout as part of Generation Victoria, assessing the impact of time post-delivery, user, and technology used for image capture, on a range of derived placental data. Study designTen placentas were imaged by three different users and imaging technologies (iPad, iPhone, Samsung) at (0 h), 24 h, and 48 h post-delivery. Using AI-PLAX, disc size (short and long length, perimeter, area), shape (normal, abnormal), cord insertion type (central, eccentric), cord coiling, abruption (retroplacental hematoma), and meconium staining were determined. ResultsWhen analysing the maternal surface of the placenta, time in cold storage post-delivery had modest effects on placental dimensions, with decreases in the short length (24–48 h: −3.7 %), disc area (0–24 h: 4.7 % and 0–48 h: −7.4 %), and perimeter (0–48 h: −3.8 %) observed. There was marginal impact on placental dimensions when the placenta was imaged by different users, including long length (+1.9 %), disc area (+2.9 %), and perimeter (+2.0 %). Measures of placental size were not impacted by the type of technology used to capture the images. When analysing the fetal surface of the placenta, more variance in placental size measures were observed between users. Abruption detection was not affected by any parameter. Time between delivery and imaging impacted apparent meconium staining – likely reflecting changes in fetal surface colour over time. Meconium staining was not affected by technology or user. ConclusionsThis study supports the feasibility of the collection of placenta images for later morphological analysis by AI-PLAX, with measures obtained minimally influenced by time in cold storage, user imaging the placenta, or technology to capture the images.