Types Of Insect Pests Research Articles

Pyriproxyfen enhances germline stem cell proliferation and reduces reproduction in Drosophila by up-regulating juvenile hormone signaling.

Pyriproxyfen is an insect growth regulator (IGR) that is effective against various types of insect pests. However, the molecular mechanism underlying pyriproxyfen effects on insect reproduction remains unclear. Thus, in this study, we attempted to uncover the mechanisms underlying the impact of pyriproxyfen on the reproductive system of the model organism Drosophila melanogaster. A significant decrease in Drosophila reproduction was observed after pyriproxyfen treatment. The juvenile hormone (JH) titer was significantly increased (120.4%) in the ovary samples of pyriproxyfen-treated flies. Likewise, the concentrations of key enzymes and the expression of key genes related to the JH signaling pathway were also increased in the pyriproxyfen-treated group compared with the control group. Furthermore, pyriproxyfen treatment significantly increased (15.6%) the number of germline stem cells (GSCs) and significantly decreased (17%) the number of cystoblasts (CBs). However, no significant differences were observed in the number of somatic cells. We performed RNA interference (RNAi) on five key genes (Met, Tai, gce, ftz-f1, and hairy) related to the JH signaling pathway in germ cells using the germ cell-specific Gal4 driver. Interestingly, RNAi of the selected genes significantly decreased the number of both GSCs and CBs in pyriproxyfen-treated transgenic flies. These results further validate that pyriproxyfen enhances GSC proliferation by up-regulating JH signaling. Our results indicate that pyriproxyfen significantly decreases reproduction by affecting germ cells in female adult ovaries. The effect of pyriproxyfen on germ cell proliferation and differentiation is mediated by an increase in JH signaling. This study has significant implications for optimizing pest control strategies, developing sustainable agriculture practices, and understanding the mechanism of insecticide action. © 2024 Society of Chemical Industry.

The effect of paraquat dichloride and carbosulfan on soil conditions and population dynamic of soil microbes in a cornfield: a case study in Sumedang, West Java

Paraquat dichloride (PC) is one of the herbicides active ingredients that is widely used by farmers to control various types of weeds, while carbosulfan (CS) is one of the insecticide’s active ingredients that is widely used to control various types of insect pests. The objective of this study is to analyze the impact of pesticides for PC and CS on the chemical and physical conditions of the soil and the dynamics of fungi and soil bacteria population in a corn field in Sumedang district, West Java. The experiment took place between August and October, during the dry season, concluding just as the rainy season began. The PC concentrations used were 0 mL/L, 3.33 mL/L, 4 mL/L, 4.66 mL/L and 5.33 mL/L, while the CS concentrations used were 0 mL/L, 0.5 mL/L, 1 mL/L, 1.5 mL/L and 2 mL/L. Soil sampling was conducted before and after the application of pesticides. The total abundance of fungi and bacteria was analyzed using the total plate count (TPC) method. The application of PC to the cornfield slightly decreased soil bulk density from 1.354 g/cm3 to 0.816 g/cm3, while the application of CS slightly increased soil bulk density. A decrease in bulk density is considered positive for the physical conditions of the soil for plant cultivation. In general, the result indicated that the application of PC and CS did not show a significant effect on the chemical characteristics of the soil, and also it did not affect the population of fungi and bacteria. Weather conditions, i.e. dry season (June-September) and rainy season (started from October) seem to affect more on soil conditions as well soil microbial population than the application of the pesticides.

Usage of Jatropha curcas as Botanical Insecticide in Controlling Insect Pest

Insect pests can cause damage to plants and cause losses. Insect pests on plants need to be controlled both preventively and curatively. Integrated control is an integration of several control methods that can be implemented in a compatible and comprehensive. Insect pest control using bio-active plant materials as an alternative to synthetic chemicals. Plants produce secondary metabolite compounds that can affect insects, including Jatropha curcas (Euphorbiaceae). Studies on several insect pests both in the laboratory and in the field against insect pests that attack the field and agricultural product storage warehouses have been carried out to determine their effectiveness. Jatropha curcas plants/parts of plant were applied after extraction treatment with various solvents (water, ethanol, methanol, acetone, petroleum ether, n-hexane and chloroform) or direct use. The results showed that J. curcas exhibited control effects against several types of insect pests from various insect pest. Laboratory study showed the insecticidal effect of J. curcas against Helicoverpa armigera, Spodoptera frugiperda, Plutella xylostella, Earias insulata, Spilarctia oblicua, Tuta absulata, Nezara viridula, Corcyra cephalonica, Maruca vitrata. Field study showed population reduction of Putella xylostella and Aphis craccivora. Whilst, on stored product study showed mortality of insect pest (Sitophilus zeamais, Rhyzopertha dominica, Callosobruchus chinensis and C. maculatus) and reduction of weight loss of stored products. Jatropha curcas has the potential as a pest insect control to be integrated into the IPM component. Keywords: Jatropha curcas, Botanical insecticide, Insect pest

Insect Pest Diversity of Corn Plants (Zea mays) in Baringeng Village, Soppeng Regency, South Sulawesi Province

Background: Baringeng is a corn-producing village in Soppeng Regency, South Sulawesi. The main problem for corn farmers in the town is insect pests. Insect pests damage the plant, which has an impact on decreasing yields. Control of insect pests must be by the type of insect pests. This study aims to calculate the diversity of insect pests of corn in Baringeng Village and the damage they cause. Methods: Data was collected in Takku Hamlet, Baringeng Hamlet, and Tanjonge Hamlet. with a systematic plot sampling method with the help of light traps, sweep nets, and direct collection of pests. Observations included the number of individuals and types of insect pests. Data were analyzed by the Shannon-Wiener diversity index (H'), evenness index (E), dominance index (C), and similarity index. Results: There were three types of insect pests with a total of 153 individuals that attacked corn in Baringeng Village, namely grasshoppers (Oxya sp.), planthoppers (Peregrinus maidis), and armyworm (Spodoptera frugiperda). The diversity index is included in the low category (H'=0.89), the evenness index (E) is in the high sort (E=0.81), the dominance index is in the low sort (C=0.44), and the lowest similarity index shown between Dusun Baringeng and Dusun Takku (IS=50%). Conclusions: The types of insect pests found in Baringeng Village come from three different orders, namely Orthoptera, Lepidoptera, and Hemiptera. Each problem shows other attack characteristics, but all attack the leaves. So that pest control can use natural materials by spraying on leaves, polyculture, crop rotation, or taking pests directly.

Bioactivity of extracts from Syzygium aromaticum, Annona muricata, and Piper retrofractum against Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae)

Fall armyworm (FAW) Spodoptera frugiperda is an important pest on maize. One of the environmentally friendly control strategies of FAW is by utilizing botanical insecticides. Extracts of Syzygium aromaticum, Annona muricata, and Piper retrofractum are known to be able to control various types of insect pests and ensure sustainability in farming. The objective of this research was to study the effective control of three plant extracts against FAW, including the toxicity against larvae using leaf residual method, the feeding inhibition against larvae, and the oviposition deterrence against female. Probit analysis was used to estimate LC50 and LC95 values of the extracts. The extract of P. retrofractrum exhibited highest mortality compared to the other extracts with LC95 values of 0.230%, followed by S. aromaticum 0.349%, then A. muricata 1.387% against first-instar larvae at 96 hours. P. retrofractum extract showed the highest feeding inhibition of 90.14% at LC95 value, which gradually decreased on S. aromaticum and A. muricata extracts were 80.33% and 78.44%, respectively. Three plant extracts at LC99 and 2 x LC99 levels deterred female oviposition by 100%. This results indicate that P. retrofractum extract has great potential for further development as a botanical insecticide to controlling FAW.

The Diversity of Warehouse Pests on Areca Nuts in the West Sumatera, Indonesia

Areca nut is one of Indonesia’s leading export commodities that must adhere to certain phytosanitary requirements, including being pest-free. It is infested by several types of insect pests during the storage process. Therefore, this study aims to determine the diversity of warehouse pests that infest areca nuts. It was conducted using a survey method at three warehouses of large collectors and exporters of areca nut in the Padang city and the Padang Pariaman regency. The purposive sampling method was adopted to collect samples from three traders' warehouses. Furthermore, the nut was taken diagonally in a pile, namely the corner and center of 1 kg each, and repeated 4 times. The identification key was used to identify 15 insect types from 10 families and 3 orders. The Cryptolestes pusillus, C. ferugineus, and Carpophilus sp. were found with the highest number of individuals in each warehouse. The diversity index of the three warehouses is 1.78, 2.14, and 1.90 indicating a moderate level. Additionally, the similarity index between warehouses shows a high and very high level of species similarity.

Abundance and Dynamics of the Main Heteroptera Pests of Cocoa Tree in the Orchards of the Department of Méagui (South-West, Côte d’Ivoire)

The capsids Sahlbergella singularis and Distantiella theobromae, the cocoa mosquito Helopeltis sp., and the green bug Bathycoelia thalassina are the main Heteroptera pests causing immense damage in cocoa fields in the department of Méagui, the main cocoa producing area of Côte d’Ivoire. The actual study was conducted to assess the spatial and temporal distribution of these pests in this department. From May 2020 to April 2021, the pest abundance and population dynamics were recorded once a month in seven selected cocoa farms in the localities of Yaodankro and Sérigbangan. Tarping and systematic search methods were used. The results indicated that the three types of insect pests are present and the abundance rates ranging from 20.83% to 42.22% from 15 910 individuals recorded. Capsids were more abundant in the Sérigbangan orchards than in those of Yaodankro, while the cocoa mosquito and the green bug were more abundant in Yaodankro than in Sérigbangan. The number of individuals remained relatively high throughout the year except in May-June (months of intense rainfall) where capsid and cocoa mosquito populations were less abundant. Peak populations occurred during the dry season (July, August, February, and March-April) and during the low/medium rainfall season (September and November). The cocoa mosquito and the green bug once considered minor pests were shown to be major pests.

The Effect of Color Type and Light Intensity of Light Emitting Diode (LED) Light Traps on the Types and Number of Pest Insect Catches in Rice Plantations

The purpose of this study was to examine the effect of the type of color and light intensity of the light emitting diode (LED) Light traps on the type and number of insect pests in rice plantations. The research was conducted from November 2017 to March 2018, in Kencong Village, Jember Regency. The data collection method used Scan sampling, for 11 weeks, one week interval, starting at 07.00 p.m. to 04.00.a.m. The number of plots was 4 pieces, each 100 m2. The distance between the plots is 100 meters. Light traps in each plot, red, yellow, blue and white. Each color consists of 4 light intensities, namely 3 watts, 5 watts, 7 watts and 9 watts. Observation parameters: types and number of insect pests catches. The different types and numbers of insect pests caught were using the Kruskal-Wallis test. The normality of the data used the Shapiro-Wilt test. Statistical significance p < 0.05. Correlation between lamp color and light intensity, using the Spearman correlation test. Data were analyzed using SPSS version 23.0. The conclusions of the study were: The types of insect pests at various colors and intensity of LED light traps showed similarities, consisting of 5 orders, 6 families and 6 species. The red LED trap light indicates the highest number of pest catches. The number of insect pest catches of red LED was 475 individuals. A light intensity of 9 Watts indicates the highest number of insect pest catcheswere 568 individuals. The spearman correlation coefficient between the type of LED color and the light intensity (r) = - 0.415.

The influence of cropping pattern of pepper with citronella and lemongrass to the insect diversity and insect pests population

Lemongrass and Citronella contain essential oils which are repellent against insect pests. Research on the cropping pattern of pepper with lemongrass and citronella was conducted to determine the influence of two essential oil plants on the diversity and population of insect pests of pepper plants. The research was conducted at the Sukamulya Experimental Garden, from 2018 to 2020 on a 3000 m2 planting plot. The treatments of the cropping patterns tested included: 1) Climbing pepper, 2) Climbing pepper + Shrub pepper, 3) Climbing pepper + Citronella, 4) Climbing pepper + Lemongrass, 5) Climbing pepper + Shrub pepper + Citronella, 6) Climbing pepper + Shrub pepper + Lemongrass. Each treatment was repeated twice. Observation parameters were insect diversity and insect pests, the level of pest attack, and the number of plants attacked. The results obtained about 66 species of insects from 46 families and 9 orders. The insects associated with the pepper cropping pattern ecosystem were relatively evenly distributed in all treatments, except for treatment 3, where only 4 types of insect pests were found. Pests, especially Thrips sp., were distributed in all treatments, but in the combination treatment with citronella and lemongrass, the population of Thrips sp. relatively low.

Effectiveness Test of Tagetes erecta L Powder and Cattle Urine Waste that Potentially Repelent ont Boring Pests of Nezara viridula Pods in Soybean (Glycine max L) in Patumbak Subdistrict, Deli Serdang, North Sumatra

Soybean plants (Glycine max) are the third most important food commodity crop after rice and corn. Soybean production continues to be improved in meeting community needs, but efforts to increase soybean production still face problems, namely green ladybird pest (N. viridula L.). One alternative to control soybean pod sucking insects that are relatively safe, inexpensive, and easily obtained is the use of vegetable insecticides. Vegetable insecticides do not quickly cause pest resistance, are synergistic, and their use can be combined with other pest control techniques. One of them is by using Tagetes erecta plants combined with cow urine as a vegetable insecticide. The use of repellents generally does not directly kill insects, but rather serves to reject the presence of insects, mainly due to the pungent smell. The use of cow urine as a mixture of biopesticides contains growth stimulants and contains repellents for several types of insect pests. This study aims to determine the effectiveness of Tagetes erecta in reducing the population of N.viridula pests in soybean plants (Glycine max L.Merr). Non Factorial RAK with 5 levels of treatment and 5 replications. Hopefully with this study farmers can take advantage of tagetes erecta and cow urine waste that can be used as natural pesticides and can be used as a control for Hama Nezara viridula which is environmentally friendly and economic.

English

The survey was carried out in 2016 in the production of citrus fruits at Adiha and Sheka Tekli irrigation schemes of Kolla Tembien and Tanqua Abergelle districts, to assess the type of diseases, the causes and the characteristic symptoms of the diseases and to identify the types of insect pests that inflict serious damage. The laboratory analysis result depicted that bacteria and fungi caused most of the diseases of citrus at Adiha and Sheka Tekli irrigation schemes. Exceptionally cyst nematode and climbing weed were problems in production of citrus at the irrigation schemes. Diseases like citrus melanose, brown rot, root rot, blue mold, sooty mold, leaf spot and fruit spot were among the fungal diseases identified at the irrigation schemes. Moreover, citrus greening, and citrus canker were also some of the bacterial diseases of citrus at Adiha and Sheka Tekli irrigation schemes. Conversely, the type of insect pest that predominantly constrained the production of citrus in both areas were woolly whitefly, citrus mealybugs, orange dogs, cottony cushion scale insects, brown scale insect, citrus leafminer, citrus psyllid, fruit fly, adult flatid planthoppers, citrus aphid, red scale and root weevil respectively. Most of these insect pests belong to the Hemipteran order followed by lepidopteron insect group. Milk vine had also been observed as one of the weeds that pose serious problems to some of the citrus trees at Adiha and Sheka Tekli irrigation schemes. Therefore, further research intervention should focus on management of these insect and disease pests at Adiha and Sheka Tekli irrigation schemes. Key words: Citrus disease, insect pest, symptoms and identification.

Optimizing methods for rearing mated queens and establishing new colony of Oecophylla longinoda (Hymenoptera: Formicidae)

Abstract Oecophylla spp. are used as biocontrol agents for many types of insect pests. A large and stable population is essential for effective control of pests. Colonies of Oecophylla spp. can be transplanted from wild habitats into orchards. Transplanted colonies can only survive in the presence of egg laying queens. It is difficult to locate nests with egg laying queens in large colonies that may sometimes contain more than 100 nests. Therefore, the need to explore and develop methods for rearing newly mated queens in nurseries may not be over emphasized, hence the current study. In the first experiment, we tested three rearing methods on queen survival and colony establishment. In the second experiment, we compared feeding techniques of different weaver ants on young colony growth. We observed that queens were best reared under continuous, indirect access to water. The first workers emerged earlier (32 days on average) in indirect and direct continuous access to water methods than on limited access to water (sprinkled) (38 days on average). Moreover, rearing mated queens under continuous indirect access and continuous direct access to water methods saved labour and time, because of limited attendance to the colonies. Availability of water, sugar solution and different sources of protein throughout improved the growth of young colonies. Likewise, the number of workers increased rapidly. Therefore rearing mated queens in nurseries is possible and would minimize hustles in hunting for the colonies and their queens in the wilderness.

Solar Energy-Based Insect Pest Trap

Agriculture is the main occupation of Thai people. Farmers encounter the problems of various types of insect pests that harm crops and result in loss of productivity each year. Therefore, it is necessary for farmers to use pesticides to prevent crop damage. However, when pesticides are used in large quantity, they cause adverse impacts on people, animals and the environment. Instead of using pesticides, the government has to support other ways to prevent insect pests, including the use of biological agents and some insects etc. A previous study has shown that the ultraviolet light of light emitting diode tube could be used to lure “Coconut Hispine Beetle”(Plesispa reichei Chapuis), a damaging pest of coconut and a range of palm species. This study aimed to develop Solar Energy-Based Insect Pests Trap by using ultraviolet light emitting diode tube to lure the insect pests and 12 volt battery as power supply to light emitting diode tube. The battery charging system derives electrical energy from 20 watts of solar cell for use at night. This proposed Solar Energy-Based Insect Pests Trap has an automatic control system to lure insect pests when there is no sunlight and the system will be stop when the sun shines. The results of the system installation test showed that this proposed Solar Energy-Based Insect Pests Trap could lure several types of insect pests in vegetable and coconut plantations including Brotispa, Elephus beetles, and Aphis, etc.