Food Types Research Articles

Substituting potatoes with other food groups and type 2 diabetes risk: findings from the Diet, Cancer, and Health Study

Substituting potatoes with other food groups and type 2 diabetes risk: findings from the Diet, Cancer, and Health Study

Rapid Convolutional Algorithm for the Discovery of Blueberry Honey Authenticity Markers via Nontargeted LC-MS Analysis.

Bees produce honey through the collection and transformation of nectar, whose botanical origin impacts the taste, nutritional value, and, therefore, the market price of the resulting honey. This phenomenon has led some to mislabel their honey so that it can be sold at a higher price. Metabolomics has been gaining popularity in food authentication, but rapid data mining algorithms are needed to facilitate the discovery of new authenticity markers. A nontargeted high-resolution liquid chromatography-mass spectrometry (HR/LC-MS) analysis of 262 monofloral honey samples, of which 50 were blueberry honey, was performed. Data mining methods were demonstrated for the discovery of binary single-markers (compound was only detected in blueberry honey), threshold single-markers (compound had the highest concentration in blueberry honey), and interval ratio-markers (the ratio of two compounds was within a unique interval in blueberry honey). A novel convolutional algorithm was developed for the discovery of interval ratio-markers, which trained 14× faster and achieved a 0.2 Matthews correlation coefficient (MCC) units higher classification score than existing open-source implementations. The convolutional algorithm also had classification performance similar to that of a brute-force search but trained 1521× faster. A pipeline for shortlisting candidate authenticity markers from the LC-MS spectra that may be suitable for chemical structure identification was also demonstrated and led to the identification of niacin as a blueberry honey threshold single-marker. This work demonstrates an end-to-end approach to mine the honey metabolome for novel authenticity markers and can readily be applied to other types of food and analytical chemistry instruments.

A polymerase chain reaction (PCR) method to detect emerging multidrug-resistant Salmonella Infantis harbouring pESI plasmid in seafood.

Salmonella Infantis is an emerging multidrug-resistant pathogen worldwide due to the acquisition of a megaplasmid pESI (Plasmid of Emerging Salmonella Infantis). Reported initially from poultry, the distribution of pESI-harbouring S. Infantis in other food types, including seafood, is unknown. This study aimed to develop and optimize a PCR assay for detecting pESI plasmid in Salmonella and non-Salmonella Enterobacterales. A duplex PCR targeting the hilA gene and a pESI-associated gene of S. Infantis was designed, and the PCR conditions were optimized. The specificity and sensitivity of the assay were established using 119 Salmonella serovars and 51 non-Salmonella bacterial strains. All Salmonella isolates yielded hilA PCR product, while only pESI S. Infantis was positive for both hilA and pESI genes. No amplification product was obtained with the DNA of 51 non-Salmonella bacterial strains. The detection limit of the duplex PCR was 104 CFU/ml of pure culture of pESI S. Infantis. The sensitivity of detection in artificially spiked shrimp meat was 1 CFU/g after 6 h of enrichment in lactose broth, followed by 12 h of selective enrichment in the Rappaport-Vassiliadis medium. The duplex assay will help screen seafood for Salmonella in general and pESI S. Infantis in particular. Given its high sensitivity, the PCR will be a valuable tool for seafood quality assurance. This approach decreases the typical 3-6 days' identification time of Salmonella to less than 24 hours. S. Infantis carrying the highly transmissible megaplasmid (pESI) is a significant food safety concern. Given its rapid geographical spread and high antimicrobial resistant traits, it is necessary to have a molecular tool that detects pESI-harbouring Salmonella. This study successfully developed a duplex PCR assay that simultaneously detects Salmonella enterica and pESI S. Infantis. This molecular tool will help understand the distribution, sources, and spread of MDR plasmid in the food environment.

Exposure to food additive mixtures and type 2 diabetes risk in the NutriNet-Santé cohort

Abstract Background So far, the safety evaluation of food additives has been performed substance by substance, while in real life, millions of individuals are exposed to mixtures of food additives daily. According to preliminary experimental data, some of these chemicals, alone and/or in synergy are suspected to impact metabolic health. The objective of this study was to investigate for the first time the associations between exposure to mixtures of food additives and type 2 diabetes (T2D) risk in a large prospective cohort of French adults. Methods Participants (n = 108,783, 79.2% women, mean age=42.4 years, SD = 14.6) from the NutriNet-Santé cohort (2009-2024) completed repeated 24h-dietary records, detailing industrial product brands. Additive exposure was assessed using composition databases and laboratory assays. Five mixtures of additives were identified through non-negative matrix factorisation (NMF). Associations between these mixtures and the risk of T2D were characterised using multivariable proportional hazards Cox models adjusted for known risk factors. Findings 1115 participants were identified with T2D during follow-up. Two additive mixtures were associated with increased T2D risk. The first mixture included higher exposure to modified starches E14xx, pectins E440, guar gum E412, carrageenan E407, and polyphosphates E452 (HRper 1SD increment=1.11 [1.05-1.18]), p < 0.0001). The second mixture comprised citric acid E330, sodium citrates E331, phosphoric acid E338, sulphite ammonia caramel E150d, acesulfame-K E950, aspartame E951, sucralose E955 and arabic gum E414 (HR1SD=1.13 [1.08-1.18]), p < 0.0001). Interpretation This large prospective cohort study revealed direct associations between T2D risk and exposure to two food additive mixtures. Molecular epidemiology and experimental studies will be necessary to depict underlying mechanisms and potential cocktail effects. Key messages • Consumed together, widely used food additives may contribute to T2D aetiology, shedding light on the detrimental impact of highly processed foods on metabolic health. • This study highlights positives associations between T2D risk and exposure to two food additive mixtures.

Ultra-processed food intake and type 2 diabetes: A dose-response meta-analyses of prospective studies

Abstract Studies suggested a positive association between ultra-processed food (UPF) intake and risk of type 2 diabetes (T2DM). However, the magnitude of association varies across studies due to differences in population characteristics and methodological issues. It is also unclear about the exact shape of the association and whether the association is independent of diet quality and adiposity. We conducted a dose-response meta-analysis of prospective studies to summarize the association between UPF intake and T2DM risk. PubMed/MEDLINE, Web of Science, and Embase were searched through Jan. 2024 to identify relevant studies. Using random-effects models, summary relative risks (RRs) and 95% confidence intervals (CIs) were estimated for each of the following unit increment in UPF intake: 10% (of total g/d), 100-g/d, and 1-serving/d. A non-linear dose-response meta-analysis was conducted using restricted cubic spline analysis. After screening 569 publications, 12 prospective cohort studies were included in this study. The summary RRs associated with every 10%, 100-g, and 1-serving increment in UPF intake were 1.13 (95% CI = 1.10-1.16), 1.05 (95% CI = 1.03-1.06), and 1.04 (95% CI = 1.03-1.05), respectively. The positive associations remained statistically significant after additional adjustment for diet quality, BMI, and total energy intake. The dose-response curve for g/d of UPF intake suggested evidence of nonlinearity (p-nonlinearity=0.0005), showing a steeper increase in risk at UPF intake >300 g/d. However, evidence for non-linearity was not observed with % and serving/d of UPF intake. The association did not significantly vary by method and timing of UPF assessment. Higher summary RRs were observed among studies from Europe and North America compared with those from other countries (p-heterogeneity=0.048). Our data suggest that higher UPF intake may increase T2DM risk, independent of diet quality and adiposity. Key messages • Higher intake of ultra-processed food is positively associated with the risk of type 2 diabetes. • Public health strategies should encourage healthier dietary choices by emphasizing reduction of UPF intake in dietary guidelines and implementing regulations on manufacturing and labeling of UPF.

Focus on food instead of nutrients improves consumers’ understanding of meals’ nutrition quality

Abstract Background Nowadays, most of the information directed to consumers focuses on nutrients, ignoring the impact of the interactions between different foods, origin or degree of processing. The methodologies currently applied have led consumers to confuse foods with nutrients, meat and fish are often referred to as “proteins”, grains and potatoes are referred to as “carbohydrates, forgetting that these foods are source of other nutrients, such as fat, proteins or fibre. Furthermore, its efficacy is compromised by the difficulties of reading labels among consumers. Objectives This study aimed to evaluate the understanding about food and nutritional information presented in menus to consumers, comparing the nutrient-focused approach (Nutrition Declaration) and the new food-focused approach (infographic - based on the Portuguese food Guide, from previous research). Methods 4 different meals were created, 2 balanced and complete in terms of food and nutrient content and 2 unbalanced and uncomplete. A questionnaire was applied, evaluating the understanding of the balance and healthiness of the meals, using the two approaches (nutrient-focused and food-focused). The order of presentation of the approaches and the meals was randomized for all participants. The questionnaire was distributed online through snowball sampling. Results 221 individuals participated, mostly female (67%), aged 25 to 54 years old. Evaluation of meals (“how balanced” and “how complete” the meal is) using the Nutrition Declaration did not allow to distinguish the quality of meals (Mb = 3,8;Mc=4,0), in opposite the Infographic returned significant differences between M1/M2 (Mb = 4,3;Mc=4,4) and M3/M4 (Mb = 2,5;Mc=2,6) (p < 0.01) allowing consumers to perceive differences between meals. The percentage of consumers that respond “I don’t know” to these questions decreased using the food-focused tool (13% vs 2%). Conclusions The food-focused approach allows for better understanding of meals’ nutritional balance. Key messages • The infographic provides more information about the daily food portions and how different meals and types of food contribute for complying food and nutritional recommendations. • The use of the infographic in menus, recipes, cookbooks and clinical advice may improve better food choices and a healthier lifestyle.

The influence of front-of-pack labelling on consumer decision-making: a comprehensive study using electroencephalography and FaceReader

Front-of-pack labels (FOPL) can influence consumer behaviour in a variety of ways, and companies that prioritise accurate and transparent nutrition labels can see benefits in increased sales and brand loyalty. However, there are several types of FOPL on the market that differ in visual appeal and consumer clarity. The aim of the study was to reveal the impact of the nutrition label Nutri-Score as well as other factors on consumer decision-making when choosing healthier alternatives for selected food types. In addition to traditional research methods such as questionnaires, in-depth interviews, also emerging methods such as eye tracking, FaceReader and electroencephalography were used to collect implicit feedback. Research suggests that there are significant differences between implicit and explicit feedback when examining the impact of Nutri-Score on the decision-making process. Last but not least, the study presents ideas for future research in real-world settings using emerging methods.

Edible Insects: Consumption, Perceptions, Culture and Tradition Among Adult Citizens from 14 Countries

Although edible insects (EIs) are encouraged as a sustainable source of protein, their consumption is not as generalised as other types of food that are internationally accepted. While in some regions of the world, EIs are part of the gastronomic and cultural traditions, in other regions, people are not so receptive to this type of food, and some people even express some disgust towards it. Hence, this research focused on the habits of the participants regarding the consumption of insects as well as their perceptions about EIs being or not a part of the local culture or gastronomic patrimony. A questionnaire survey was implemented in fourteen countries (Brazil, Croatia, Greece, Latvia, Lebanon, Lithuania, Mexico, Poland, Portugal, Romania, Serbia, Slovenia, Spain, and Turkey), and globally, 7222 adult participants responded to the questionnaire. SPSS software (version 28) was used to process the data and carry out chi-square tests and Factor Analyses (FA). The obtained results showed significant differences between countries for all the questions included in the survey, either those regarding the habits of the participants or their opinions about the facts linked with EI tradition or cultural aspects. It was found that participants from Mexico consume EIs more than in all other countries and that strong motivations that would lead to consumption among those who do not consume include curiosity and food shortage. The solution obtained with FA considering the ten statements of the scale consisted of two factors: F1—Culture and Tradition of EIs (α = 0.675) and F2—Acceptance of EIs (α = 0.614). In conclusion, the consumption of EIs and the perceptions of people are highly variable according to geographic location and cultural environment.

Performance Characteristics of the Quantitative Method for Staphylococcus aureus in Food Products Correspond to ISO 16140-3: 2021

Staphylococcus aureus is a pathogenic bacterium that poses significant health risks when present in food products. High levels of Staphylococcus aureus in food can indicate poor hygiene and handling practices during food production, processing, or storage. In order to maintain food safety and quality, verification of the quantitative method of Staphylococcus aureus is very important. Method verification represents a crucial stage in guaranteeing that all employed procedures conform to the necessary criteria for dependable and precise data. Following the guidelines established in ISO 16140-3:2021, method verification is accomplished through the calculation of the Intralaboratory Reproducibility Standard Deviation (SIR) for implementation validation and the eBias value for verifying the suitability of the method for assessing specific food items. This research was conducted with the objective of confirming the ISO 6888-1:2021 as the designated reference method for quantifying Staphylococcus aureus in food products. For the purpose of confirming the implementation of the method, Cheese was utilized as the test food item. In the context of verifying the method's applicability to different food products, a range of items including condensed milk, margarine, baby porridge, cassava chips and ready-to-eat sausage were examined as challenging foods. Every chosen food item was artificially contaminated with Staphylococcus aureus WDCM 00034. The SIR value obtained was 0.04 ≤ 2× 0.11 (the lowest mean of SR value from ISO 6888-1:2021) which indicated that the National Quality Control Laboratory of Drug and Food (NQCLDF), Indonesian FDA was able to implement the method very well. In addition, the eBias value for all types of food tested was below 0.5log10, which showed that the quantitative method for coagulase-positive Staphylococci (Staphylococcus aureus and other types) could be applied in the NQCLDF laboratory for the extensive scope of food.

Fibre & fermented foods: differential effects on the microbiota-gut-brain axis.

The ability to manipulate brain function through the communication between the microorganisms in the gastrointestinal tract and the brain along the gut-brain axis has emerged as a potential option to improve cognitive and emotional health. Dietary composition and patterns have demonstrated a robust capacity to modulate the microbiota-gut-brain axis. With their potential to possess pre-, pro-, post-, and synbiotic properties, dietary fibre and fermented foods stand out as potent shapers of the gut microbiota and subsequent signalling to the brain. Despite this potential, few studies have directly examined the mechanisms that might explain the beneficial action of dietary fibre and fermented foods on the microbiota-gut-brain axis, thus limiting insight and treatments for brain dysfunction. Herein, we evaluate the differential effects of dietary fibre and fermented foods from whole food sources on cognitive and emotional functioning. Potential mediating effects of dietary fibre and fermented foods on brain health via the microbiota-gut-brain axis are described. Although more multimodal research that combines psychological assessments and biological sampling to compare each food type is needed, the evidence accumulated to date suggests that dietary fibre, fermented foods, and/or their combination within a psychobiotic diet can be a cost-effective and convenient approach to improve cognitive and emotional functioning across the lifespan.

Restoring historical moose densities results in fewer wolves killed for woodland caribou conservation

AbstractWoodland caribou (Rangifer tarandus caribou) are declining across much of their distribution in Canada in response to habitat alteration, leading to unsustainable predation, particularly by wolves (Canis lupus). Habitat alteration can benefit the primary prey species of wolves (moose [Alces alces] and deer [Odocoileus spp.]) by creating early seral conditions that contain more of their preferred food types. This increase in primary prey populations results in elevated wolf abundance and heightened predation pressure on caribou. In response to the elevated wolf populations and the risks to caribou, managers have reduced wolf abundance in key areas. Ecological theory suggests that reducing wolf abundance would release moose from the top‐down effects of wolf predation, potentially allowing moose populations to grow. Elevated moose abundance thus has the potential to cause wolf populations to rebound quickly each year following reductions, suggesting a possible link between moose abundance and the number of wolves killed for caribou conservation. To test this idea we used a unique management situation in British Columbia and Alberta, Canada, where lethal wolf removals were annually conducted across specific southern mountain caribou population ranges and, in some places, moose populations were concurrently reduced via liberalized hunting. We used indices of moose abundance and wolf removal data to test the hypothesis that reducing moose populations to a historical abundance target by hunting leads to fewer wolves killed for caribou conservation. After controlling for habitat quality, wolves removed per km2 was 3.2 times lower in areas with reduced moose density ( = 1.55 wolves/1,000 km2 ± 0.33 [SE]) than in those without reduced moose density ( = 5.02 wolves/1,000 km2 ± 0.52). However, the average number of wolves removed per year decreased under both conditions. After 9 years, there was a 35% reduction in the predicted difference in the annual removal between areas with and without moose reduction. Our results suggest that policies that do not reduce or stabilize moose abundance will result in the removal of more wolves to increase caribou abundance. Like wolf reductions, moose reductions can also be controversial and affect local harvesters. Thus, understanding the consequences of actions that support caribou recovery is essential to supporting evidence‐based policy discussions.

Positive Deviance in Nutritional Practices for Two-Year-Olds in Poor Fisherman Families in the Belawan Health Center Area, 2022

The problem of malnutrition is still the main problem faced in human resource development. The problem of growth and development of Baduta is a serious problem because at this age the process of physical and intellectual growth and development is critical. Malnutrition problems mostly occur in poor families but are not caused by poor families who have well-nourished children. The Belawan Community Health Center working area is one of the community health centers with the highest number of cases of malnutrition in Medan City. To overcome this, a program is needed that is believed to be effective and sustainable. One of the existing programs to overcome the problem of malnutrition and malnutrition is positive deviation. This research aims to identify positive behavior in feeding (type of food, processing of food ingredients, mealtimes and method of feeding) of toddlers who are well-nourished in poor fishing families in the Belawan Community Health Center Working Area in 2022. This research method is qualitative research with a narrative approach. Researchers studied individual lives by asking one or several individuals to tell stories about their lives related to positive behavior in feeding well-nourished animals. This information is then retold by the researcher in a narrative chronology. The findings of this research indicate that addressing nutritional challenges in children can be effectively achieved by leveraging the potential and resources within the community. This is done through the identification of positive deviant behaviors, particularly those related to beneficial feeding practices. These practices encompass aspects such as the types of food provided, the methods of food preparation, the timing of meals, and the approach to food administration. Keep up the great work! The way you’re thinking about integrating community strengths is spot-on for creating sustainable solutions.

A systematic review and quality assessment of estimated daily intake of microplastics through food.

Plastic waste enters the oceans and soil and is consumed by organisms and humans. Some of the ingested microplastics may remain in the human body and cause toxicity. We conducted a systematic review to estimate the extent to which humans are exposed to microplastics through consumption and performed a quality assessment of research results. We searched for studies published up to December 2023 and included studies that reported on the characteristics and estimated intake of microplastics. The quality assessment tool reported in previous studies was used for food and drinking water studies. We included 76 studies in the analysis, and the types of foods were classified into seven categories: seafood, drinking water, table salt, fruits and vegetables, beverages, condiments, and meat. Theestimated daily intake of microplastics via food was 0.0002-1,531,524 MP/day, with the highest value in bottled water. The quality of food and drinking water studies was evaluated using a quantitative tool to assess reliability. The quality of food studies was 11.50 out of 20 points and the quality of drinking water studies was 11.16 out of 19 points. These results indicate that the closer the score is to the maximum, the more reliable the research findings. The quantitative assessment can be used as an indicator for evaluating the risks of microplastics and can help reduce biases that may occur during the research process. This study confirmed microplastics in foods and human exposure to up to one million microplastics daily. Our study emphasizes the potential for microplastic exposure through food intake and subsequent accumulation in the human body; therefore, efforts are needed to reduce exposure to microplastics in daily life.

Orthorexia Nervosa - unhealthy obsession on healthy lifestyle

Introduction: Eating disorders have expanded rapidly in recent years, with social media platforms like Instagram and TikTok playing a significant role. These platforms often showcase idealized images of healthy eating and body image, exposing young people to an overwhelming amount of information. This overstimulation can contribute to the development of various eating disorders. In our study, we focus particularly on orthorexia nervosa. Review methods: Our study was conducted as a literature review, with data gathered from PubMed and Embase. The state of knowledge: Orthorexia Nervosa (ON), introduced by Steven Bratman in 1997, could be defined as an unhealthy obsession with healthy eating. This obsession leads to strict dietary restrictions, where individuals often exclude certain food types, such as preservatives and GMOs, and experience significant stress and anxiety over food choices, planning, and preparation methods. The fixation on food purity can result in social isolation, anxiety, and major disruptions to daily life. In comparison to other eating disorders like anorexia nervosa and obsessive-compulsive disorder (OCD), ON is characterized by its focus on food quality rather than body image or weight. Conclusions: The classification of orthorexia as a mental disorder and the need for its recognition in DSM-5 are still debated. However, growing concern about its impact on mental and physical health has led to increased research and awareness.

Mode of action exploration for prostate epithelial cell injury caused by bisphenol A

Bisphenol A (BPA) is a typical food chemical contaminant with various detrimental effects, especially on reproductive system. Male prostate damage is also one of its major adverse health effects, of which mode of action (MOA) remains unclear. This study aims to explore the MOA for prostate toxicity of BPA using human normal prostate epithelial cell RWPE-1 for 28-day human-relevant-level exposure. A physiological based pharmacokinetic model was used to determine the concentration of BPA based on the actual oral exposure in China. The possible key events were identified by high-throughput transcriptome sequencing and validated by qPCR, Western blot and cell cycle assay, and the benchmark concentration analysis were conducted. The enriched KEGG pathways include the endocytosis, cell cycle, cellular senescence, MAPK and TNF signaling pathways. With increasing BPA concentrations, the increased mRNA and/or protein expressions of MAPKAPK2, c-JUN and c-fos in the MAPK signaling pathway, the increased mRNA expressions of CCND1 and CDKN1A, the decreased mRNA expression of CDC25C, the increased proportion of G0/G1 phase and S phase, as well as the decreased proportion of G2/M phase, were observed. The lowest value of benchmark concentration lower confidence limit (BMCL) was retrieved from G2/M phase ratio, with 110.580 and 175.862 nM for BMCL5 and BMCL10, respectively, much higher than the male gonad maximum concentration of 0.019 nM of BPA at the current exposure level of adult Chinese males. In conclusion, the MOA of BPA induced male prostatic toxicity at human-relevant levels may include: key event (KE)1-MAPK signaling pathway activation, KE2-disorder of cell cycle regulatory gene expression (increased expression of CCND1 and CDKN1A, decreased expression of CDC25C), and KE3-disturbance of cell cycle (increased proportion of G0/G1 and S phases, decreased proportion of G2/M phases). However, more studies are needed to validate and complete the MOA.

ANALISIS MODEL PEMBELAJARAN PjBL DALAM MEMBANGUN PEMBELAJARAN MATEMATIKA DAN PENERAPAN P5 PADA KURIKULUM MERDEKA

The research, which focuses on Project Based Learning (PjBL), aims to foster character and learning independence in students and still refers to the Pancasila Student Profile. This study uses a qualitative descriptive methodology. The sample in this study was taken using non-probability sampling techniques. In accordance with the findings of the research using a project-based learning model, the initiative to improve the profile of Pancasila students at SMP Negeri 3 Sungai Pinyuh was carried out with a project-based learning model. This can be seen from the activities in the P5 activity with the theme of Local Wisdom, namely the manufacture of West Kalimantan's typical food, Spicy Porridge. In addition, the Project Based Learning learning model can be applied to mathematics learning as a novelty in mathematics learning, namely the creation of posters for grade VII ratio materials

Nano-sized gold particles driven interfacial reaction for the enhanced electrochemical nitrite sensing

Nitrite is a typical food additive and chemical fertilizer, which could pose toxicity to human and aquatic organisms when it is excessive. Therefore, it is meaningful to develop reliable analytical methods for nitrite detection. In this work, an innovative electrochemical nitrite sensor has been fabricated using In2O3 decorated with high-activity nano-sized Au particles (Au/In2O3), which were synthesized through a two-step synthetic method combining hydrothermal reaction and deposition-precipitation, resulting in an excellent sensing performance for the nitrite. Specifically, the electrochemical nitrite sensor based on Au/In2O3 exhibits high sensitivity with a linear detection range from 10 μM to 5 mM at pH 7.38. Additionally, the Au/In2O3 based nitrite sensor shows a detection limit of 0.46 μM and presents impressive selectivity and stability. The extraordinary nitrite sensing performance can be attributed to the excellent catalytic activity of nano-sized Au particles and the strong interfacial reaction between Au/In2O3 and nitrite. Furthermore, theoretical calculations reveal the interfacial reactions mechanism driven by Au particles, which enhance the charge transfer between Au/In2O3 and nitrite and thus improve its electrochemical sensing performance. This work presents a novel approach towards fabricating high-performance electrochemical nitrite sensor and reveals the enhanced interfacial reaction mechanism of nano-sized Au particles.

Egg White Diet Induces Severe Allergic Enteritis in an Animal Model Driven by Caspase-3 and Gasdermin C-Mediated Mucosal Alarmin Secretion.

Allergic enteritis is an important phenotype of food allergies. However, there is not a suitable animal model for deeply exploring the natural progression and mechanism of allergic enteritis. In our study, we successfully developed an allergic enteritis animal model by feeding mice with an egg white diet. Following the dietary challenge, allergic mice displayed typical food allergy manifestations, including decreased core temperature, aversion to the allergenic diet, and elevated levels of serum sIgE and mMCP-1. Notably, these dietary challenged mice exhibited severe gut damage, characterized by disrupted intestinal microstructure, tissue inflammation, and edema that were evident morphologically. Moreover, upon exposure to food allergens, we observed a marked increase in caspase-3 and GSDMC levels in allergic mice. These two active proteins were found to be colocalized in damaged mucosal enterocytes and were associated with the secretion of epithelial sourced alarmins, such as IL25 and TSLP. Further data on the cellular and molecular levels suggest that such severe food-induced enteritis is mediated by the caspase-3-GSDMC pathway. We believe that this established animal model provides a valuable tool for advancing research on the mechanisms of food allergies.

Improving SME Performance Through the Utilization of Usable Digital Technology in the Gunung Kawi Area, Wonosari, Malang

The development of digital technology has changed the way society consumes information and makes decisions, including in the tourism sector and other related fields, including small and medium enterprises (SMEs) around the tourist areas. The Mount Kawi area, known for its religious tourism, natural beauty, and cultural heritage, seems to continue to attract visitors even though it has not fully recovered as it was before the Covid-19 pandemic. This also impacts the development of the performance of existing SMEs. This community service aims to introduce user-friendly digital marketing technology to small and medium enterprises (SMEs), so they do not have to rely solely on the local market. The approach used is the initiation model, which involves stages of introduction, demonstration, evaluation, improvement, and reinforcement. The participants of the event consisted of 20 SMEs, ranging from batik to various types of food. The instruments for success used are observation sheets and portfolios. The results of the activities show an increase in digital knowledge among SMEs, particularly in utilizing social media for product content that supports online marketing strategies, as well as the ability to create content and operate digital technology based on market feasibility. The recommendation from this community service activity is the need for assistance in certifying their products to enhance consumer trust.

Positive perceptions and purchase of sustainable foods is associated with higher diet quality in Australian university students

Purpose This study aims to understand university students' perception and engagement with sustainable food practices and the relationship with diet quality. Design/methodology/approach A cross-sectional survey assessed Australian university students' sustainable food perceptions and purchasing behaviours, diet quality using the validated Australian Recommended Food Score and attitudes towards on campus sustainable food options. Findings Of respondents (n = 197; 63% female), over half (58%) perceived it was important to purchase sustainable foods. These students were eight times more likely report purchasing sustainable foods (OR: 8.1; 95%CI 4.2–15.7; SE: 0.3; p < 0.001) and had significantly higher diet quality (Beta coefficient: 2.9; 95% Confidence Intervals 0.4–5.4; Standard Error: 1.3; p = 0.024). Students who reported frequently purchasing all types of sustainable foods, except organic foods, had significantly higher diet quality. Few students perceived there were sufficient sustainable food choices on campus (19%), but most supported the development of an edible campus (80%). Originality/value The results highlight the potential impact of promoting sustainable food options and creating a supportive campus food environment towards improving students’ diet quality.